Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859802
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4500nts
Open reading frame: 1 to 4500
ATGATGGCAAACTGGGCGGAAGCAAGACCTCTCCTCATTCTTATTGTTTTATTAGGGCAA
TTTGTCTCAATAAAAGCCCAGGAAGAAGACGAGGATGAAGGATATGGTGAAGAAATAGCC
TGCACTCAGAATGGCCAGATGTACTTAAACAGGGACATTTGGAAACCTGCCCCTTGTCAG
ATCTGTGTCTGTGACAATGGAGCCATTCTCTGTGACAAGATAGAATGCCAGGATGTGCTG
GACTGTGCCGACCCTGTAACGCCCCCTGGGGAATGCTGTCCTGTCTGTTCACAAACACCT
GGAGGTGGCAATACCAATTTTGGTAGAGGAAGAAAGGGACAAAAGGGAGAACCAGGATTA
GTGCCTGTTGTAACAGGCATACGTGGTCGTCCAGGACCGGCAGGACCTCCAGGATCACAG
GGACCAAGAGGAGAGCGAGGGCCAAAAGGAAGACCTGGCCCTCGTGGACCTCAGGGAATT
GATGGAGAACCAGGTGTTCCTGGTCAACCTGGTGCTCCAGGACCTCCTGGACATCCGTCC
CACCCAGGACCCGATGGCTTGAGCAGGCCGTTTTCAGCTCAAATGGCTGGGTTGGATGAA
AAATCTGGACTTGGGAGTCAAGTAGGACTAATGCCTGGCTCTGTGGGTCCTGTTGGCCCA
AGGGGACCACAGGGTTTACAAGGACAGCAAGGTGGTGCAGGACCTACAGGACCTCCTGGT
GAACCTGGTGATCCTGGACCAATGGGTCCGATTGGTTCACGTGGACCAGAGGGCCCTCCT
GGTAAACCTGGGGAAGATGGTGAACCTGGCAGAAATGGAAATCCTGGTGAAGTGGGATTT
GCAGGATCTCCGGGAGCTCGTGGATTTCCTGGGGCTCCTGGTCTTCCAGGTCTGAAGGGT
CACCGAGGACACAAAGGTCTTGAAGGCCCTAAAGGTGAAGTTGGAGCACCTGGTTCCAAG
GGTGAAGCTGGCCCCACTGGTCCAATGGGTGCCATGGGTCCTCTGGGTCCGAGGGGAATG
CCAGGAGAGAGAGGGAGACTTGGGCCACAGGGTGCTCCTGGACAACGAGGTGCACATGGT
ATGCCTGGAAAACCTGGACCAATGGGTCCTCTTGGGATACCAGGCTCTTCTGGTTTTCCA
GGAAATCCTGGAATGAAGGGAGAAGCAGGTCCTACAGGGGCGCGAGGCCCTGAAGGTCCT
CAGGGGCAGAGAGGTGAAACTGGGCCCCCAGGTCCAGTTGGCTCTCCAGGTCTTCCTGGT
GCAATAGGAACTGATGGTACTCCTGGTGCCAAAGGCCCAACGGGCTCTCCAGGTACCTCT
GGTCCTCCTGGCTCAGCAGGGCCTCCTGGATCTCCAGGACCTCAGGGTAGCACTGGTCCT
CAGGGAATTCGAGGCCAACCGGGTGATCCAGGAGTTCCAGGTTTCAAAGGAGAAGCTGGC
CCAAAAGGGGAACCAGGGCCACATGGTATTCAGGGTCCGATAGGCCCACCCGGTGAAGAA
GGCAAAAGAGGTCCCAGAGGTGACCCAGGAACAGTTGGTCCTCCAGGGCCAGTGGGAGAA
AGGGGTGCTCCTGGCAATCGTGGTTTTCCAGGCTCTGATGGTTTACCTGGGCCAAAGGGT
GCTCAAGGAGAACGGGGTCCTGTAGGTTCTTCAGGACCCAAAGGAAGCCAGGGGGATCCA
GGACGTCCAGGGGAACCTGGGCTTCCAGGTGCTCGGGGTTTGACAGGAAATCCTGGTGTT
CAAGGTCCTGAAGGAAAACTTGGACCTTTGGGTGCGCCAGGGGAAGATGGCCGTCCAGGT
CCTCCAGGCTCCATAGGAATCAGAGGGCAGCCCGGGAGCATGGGCCTTCCAGGCCCCAAA
GGTAGCAGTGGTGACCCTGGGAAACCTGGAGAAGCAGGAAATGCTGGAGTTCCTGGGCAG
AGGGGAGCTCCTGGAAAAGATGGTGAAGTTGGTCCTTCTGGTCCTGTGGGCCCGCCGGGT
CTAGCTGGTGAAAGAGGAGAACAAGGACCTCCAGGCCCCACAGGTTTTCAGGGGCTTCCT
GGTCCTCCAGGGCCTCCTGGAGAAGGTGGAAAACCAGGTGATCAAGGTGTTCCTGGAGAT
CCCGGAGCAGTTGGCCCGTTAGGACCTAGAGGAGAACGAGGAAATCCTGGGGAAAGAGGA
GAACCTGGGATAACTGGACTCCCTGGTGAGAAGGGAATGGCTGGAGGACATGGTCCTGAT
GGCCCAAAAGGCAGTCCAGGTCCATCTGGGACCCCTGGAGATACAGGCCCACCAGGTCTT
CAAGGTATGCCGGGAGAAAGAGGAATTGCAGGAACTCCTGGCCCCAAGGGTGACAGAGGT
GGCATAGGAGAAAAAGGTGCTGAAGGCACAGCTGGAAATGATGGTGCAAGAGGTCTTCCA
GGTCCTTTGGGCCCTCCAGGTCCGGCAGGTCCTACTGGAGAAAAGGGTGAACCTGGTCCT
CGAGGTTTAGTTGGCCCTCCTGGCTCCCGGGGCAATCCTGGTTCTCGAGGTGAAAATGGG
CCAACTGGAGCTGTTGGTTTTGCCGGACCCCAGGGTCCTGACGGACAGCCTGGAGTAAAA
GGTGAACCTGGAGAGCCAGGACAGAAGGGAGATGCTGGTTCTCCTGGACCACAAGGTTTA
GCAGGATCCCCTGGCCCTCATGGTCCTAATGGTGTTCCTGGACTAAAAGGTGGTCGAGGA
ACCCAAGGTCCGCCTGGTGCTACAGGATTTCCTGGTTCTGCGGGCAGAGTTGGACCTCCA
GGCCCTGCTGGAGCTCCAGGACCTGCGGGACCCCTAGGGGAACCCGGGAAGGAGGGACCT
CCAGGTCTTCGTGGGGACCCTGGCTCTCATGGGCGTGTGGGAGATCGAGGACCAGCTGGC
CCCCCTGGTGGCCCAGGAGACAAAGGGGACCCAGGAGAAGATGGGCAACCTGGTCCAGAT
GGCCCCCCTGGTCCAGCTGGAACGACCGGGCAGAGAGGAATTGTTGGCATGCCTGGGCAA
CGTGGAGAGAGAGGCATGCCCGGCCTACCAGGCCCAGCGGGAACACCAGGAAAAGTAGGA
CCAACTGGTGCAACAGGAGATAAAGGTCCACCTGGACCTGTGGGGCCCCCAGGCTCCAAT
GGTCCTGTAGGGGAACCTGGACCAGAAGGTCCAGCTGGCAATGATGGTACCCCAGGACGG
GATGGTGCTGTTGGAGAACGTGGTGATCGTGGAGACCCTGGGCCTGCAGGTCTGCCAGGC
TCTCAGGGTGCCCCTGGAACTCCTGGCCCTGTGGGTGCTCCAGGAGATGCAGGACAAAGA
GGAGATCCGGGTTCTCGGGGTCCTATAGGACCACCTGGTCGAGCTGGGAAACGTGGATTA
CCTGGACCCCAAGGACCTCGTGGTGACAAAGGTGATCATGGAGACCGAGGTGACAGAGGT
CAGAAGGGCCACAGAGGCTTTACTGGTCTTCAGGGTCTTCCTGGCCCTCCTGGTCCAAAT
GGTGAACAAGGAAGTGCTGGAATCCCTGGACCATTTGGCCCAAGAGGTCCTCCAGGCCCA
GTTGGTCCTTCAGGTAAAGAAGGAAACCCTGGGCCACTTGGGCCAATTGGACCTCCAGGT
GTACGAGGCAGTGTAGGAGAAGCAGGACCTGAGGGCCCTCCTGGTGAGCCTGGCCCACCT
GGCCCTCCGGGTCCCCCTGGCCACCTTACAGCTGCTCTTGGGGATATCATGGGGCACTAT
GATGAAAGCATGCCAGATCCACTTCCTGAGTTTACTGAAGATCAGGCGGCTCCTGATGAC
AAAAACAAAACGGACCCAGGGGTTCATGCTACCCTGAAGTCACTCAGTAGTCAGATTGAA
ACCATGCGCAGCCCCGATGGCTCGAAAAAGCACCCAGCCCGCACGTGTGATGACCTAAAG
CTTTGCCATTCCGCAAAGCAGAGTGGTGAATACTGGATTGATCCTAACCAAGGATCTGTT
GAAGATGCAATCAAAGTTTACTGCAACATGGAAACAGGAGAAACATGTATTTCAGCAAAC
CCATCCAGTGTACCACGTAAAACCTGGTGGGCCAGTAAATCTCCTGACAATAAACCTGTT
TGGTATGGTCTTGATATGAACAGAGGGTCTCAGTTCGCTTATGGAGACCACCAATCACCT
AATACAGCCATTACTCAGATGACTTTTTTGCGCCTTTTATCAAAAGAAGCCTCCCAGAAC
ATCACTTACATCTGTAAAAACAGTGTAGGATACATGGACGATCAAGCTAAGAACCTCAAA
AAAGCTGTGGTTCTCAAAGGGGCAAATGACTTAGATATCAAAGCAGAGGGAAATATTAGA
TTCCGGTATATCGTTCTTCAAGACACTTGCTCTAAGCGGAATGGAAATGTGGGCAAGACT
GTCTTTGAATATAGAACACAGAATGTGGCACGCTTGCCCATCATAGATCTTGCTCCTGTG
GATGTTGGCGGCACAGACCAGGAATTCGGCGTTGAAATTGGGCCAGTTTGTTTTGTGTTG
Protein sequence predicted based on nucleotide sequence
MMANWAEARPLLILIVLLGQFVSIKAQEEDEDEGYGEEIACTQNGQMYLNRDIWKPAPCQ ICVCDNGAILCDKIECQDVLDCADPVTPPGECCPVCSQTPGGGNTNFGRGRKGQKGEPGL VPVVTGIRGRPGPAGPPGSQGPRGERGPKGRPGPRGPQGIDGEPGVPGQPGAPGPPGHPS HPGPDGLSRPFSAQMAGLDEKSGLGSQVGLMPGSVGPVGPRGPQGLQGQQGGAGPTGPPG EPGDPGPMGPIGSRGPEGPPGKPGEDGEPGRNGNPGEVGFAGSPGARGFPGAPGLPGLKG HRGHKGLEGPKGEVGAPGSKGEAGPTGPMGAMGPLGPRGMPGERGRLGPQGAPGQRGAHG MPGKPGPMGPLGIPGSSGFPGNPGMKGEAGPTGARGPEGPQGQRGETGPPGPVGSPGLPG AIGTDGTPGAKGPTGSPGTSGPPGSAGPPGSPGPQGSTGPQGIRGQPGDPGVPGFKGEAG PKGEPGPHGIQGPIGPPGEEGKRGPRGDPGTVGPPGPVGERGAPGNRGFPGSDGLPGPKG AQGERGPVGSSGPKGSQGDPGRPGEPGLPGARGLTGNPGVQGPEGKLGPLGAPGEDGRPG PPGSIGIRGQPGSMGLPGPKGSSGDPGKPGEAGNAGVPGQRGAPGKDGEVGPSGPVGPPG LAGERGEQGPPGPTGFQGLPGPPGPPGEGGKPGDQGVPGDPGAVGPLGPRGERGNPGERG EPGITGLPGEKGMAGGHGPDGPKGSPGPSGTPGDTGPPGLQGMPGERGIAGTPGPKGDRG GIGEKGAEGTAGNDGARGLPGPLGPPGPAGPTGEKGEPGPRGLVGPPGSRGNPGSRGENG PTGAVGFAGPQGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPHGPNGVPGLKGGRG TQGPPGATGFPGSAGRVGPPGPAGAPGPAGPLGEPGKEGPPGLRGDPGSHGRVGDRGPAG PPGGPGDKGDPGEDGQPGPDGPPGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKVG PTGATGDKGPPGPVGPPGSNGPVGEPGPEGPAGNDGTPGRDGAVGERGDRGDPGPAGLPG SQGAPGTPGPVGAPGDAGQRGDPGSRGPIGPPGRAGKRGLPGPQGPRGDKGDHGDRGDRG QKGHRGFTGLQGLPGPPGPNGEQGSAGIPGPFGPRGPPGPVGPSGKEGNPGPLGPIGPPG VRGSVGEAGPEGPPGEPGPPGPPGPPGHLTAALGDIMGHYDESMPDPLPEFTEDQAAPDD KNKTDPGVHATLKSLSSQIETMRSPDGSKKHPARTCDDLKLCHSAKQSGEYWIDPNQGSV EDAIKVYCNMETGETCISANPSSVPRKTWWASKSPDNKPVWYGLDMNRGSQFAYGDHQSP NTAITQMTFLRLLSKEASQNITYICKNSVGYMDDQAKNLKKAVVLKGANDLDIKAEGNIR FRYIVLQDTCSKRNGNVGKTVFEYRTQNVARLPIIDLAPVDVGGTDQEFGVEIGPVCFVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
