Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859807
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5073nts
Open reading frame: 1 to 5073
ATGTGGTCTCTGCACATAGTACTAATGAGGTGCTCCTTCAGATTGACCAAGTCCTTGGCC
ACAGGTCCCTGGTCACTTATACTCATTCTCTTTTCTGTACAATATGTATATGGGAGTGGA
AAGAAATACATTGGTCCTTGTGGAGGAAGAGATTGCTCTGTTTGCCACTGTGTTCCTGAA
AAGGGGTCTCGGGGTCCACCAGGACCACCAGGGCCACAGGGTCCAATTGGACCCCTGGGA
GCCCCAGGACCCATTGGGCTTTCAGGAGAGAAAGGAATGAGAGGGGACCGCGGCCCTCCT
GGAGCAGCAGGGGACAAAGGAGATAAGGGTCCAACTGGTGTTCCTGGATTTCCAGGTTTA
GATGGCATACCTGGGCACCCAGGGCCTCCTGGACCCAGAGGCAAACCTGGTATGAGTGGC
CACAATGGCTCAAGAGGTGACCCAGGGTTTCCAGGAGGAAGAGGAGCTCTTGGCCCAGGA
GGCCCCCTAGGCCATCCTGGGGAAAAGGGAGAAAAAGGAAATTCAGTGTTCATTTTAGGT
GCCGTTAAAGGTATTCAGGGAGACAGAGGGGACCCAGGACTGCCTGGCTTACCAGGATCT
TGGGGTGCAGGAGGACCGGCAGGTCCCACAGGATATCCTGGAGAGCCAGGGTTAGTGGGA
CCTCCGGGCCAACCAGGGCGTCCAGGTTTGAAGGGAAATCCCGGTGTGGGAGTAAAGGGG
CAAATGGGAGACCCGGGTGAGGTTGGTCAGCAAGGTTCTCCTGGACCCACCCTGTTGGTA
GAGCCACCTGACTTTTGTCTCTATAAAGGAGAAAAGGGTATAAAAGGAATTCCTGGAATG
GTTGGACTGCCAGGACCACCAGGACGCAAGGGAGAATCTGGTATTGGGGCAAAAGGAGAA
AAAGGTATTCCTGGATTTCCAGGGCCTCGGGGGGATCCTGGTTCCTATGGATCTCCAGGT
TTTCCAGGATTAAAGGGAGAACTAGGACTGGTTGGAGATCCTGGGCTATTTGGATTAATT
GGCCCAAAGGGGGATCCTGGAAATCGAGGGCACCCAGGACCACCAGGTGTTTTGGTGACT
CCACCTCTTCCACTCAAAGGCCCACCAGGGGACCCAGGGTTCCCTGGCCGCTATGGAGAA
ACAGGGGATGTTGGACCACCTGGTCCCCCAGGTCTCTTGGGCAGACCAGGGGAAGCCTGT
GCAGGCATGATAGGACCCCCTGGGCCACAAGGATTTCCTGGTCTTCCTGGGCTTCCAGGA
GAAGCTGGTATTCCTGGGAGACCTGATTCTGCTCCAGGAAAACCAGGGAAGCCAGGATCA
CCTGGCTTGCCTGGAGCACCAGGCCTGCAGGGCCTCCCAGGATCAAGTGTGATATACTGT
AGTGTTGGGAACCCCGGACCACAAGGAATAAAAGGCAAAGTTGGTCCCCCAGGAGGAAGA
GGCCCAAAAGGAGAAAAAGGAAATGAAGGACTCTGTGCCTGTGAGCCTGGACCCATGGGC
CCCCCTGGCCCTCCAGGACTTCCTGGGAGGCAGGGGAGTAAGGGAGACTTGGGGCTCCCT
GGCTGGCTTGGAACAAAAGGTGACCCAGGACCTCCTGGTGCTGAAGGACCTCCAGGGCTA
CCAGGAAAGCATGGTGCCTCTGGACCACCTGGCAACAAAGGGGCGAAGGGTGACATGGTT
GTATCAAGAGTTAAAGGGCACAAAGGAGAAAGAGGTCCTGATGGGCCCCCAGGATTTCCA
GGGCAGCCAGGATCACATGGTCGGGATGGACATGCTGGAGAAAAAGGGGATCCAGGACCT
CCAGGGGATCATGAAGATGCGACCCCAGGTGGTAAAGGATTTCCTGGACCTCTGGGCCCC
CCAGGCAAAGCAGGACCTGTGGGGCCCCCAGGACTGGGATTTCCTGGTCCACCAGGAGAG
CGAGGCCACCCAGGAGTTCCAGGCCACCCAGGTGTGAGGGGCCCTGATGGCTTGAAGGGT
CAGAAAGGTGACACAATTTCTTGCAACGTAACCTACCCTGGGAGGCATGGCCCTCCAGGT
TTTGATGGACCTCCAGGTCCGAAGGGATTTCCAGGTCCCCAAGGTGCCCCTGGGCTGAGT
GGTTCAGATGGGCATAAAGGCAGACCTGGCACACCAGGAACAGCGGAAATACCAGGTCCA
CCTGGTTTTCGTGGTGACATGGGAGATCCGGGTTTTGGAGGTGAAAAGGGGTCCTCCCCT
GTTGGGCCCCCAGGCCCTCCCGGCTCACCAGGAGTGAATGGTCAGAAAGGAATCCCGGGA
GACCCTGCATTTGGTCACCTGGGACCCCCGGGAAAGAGGGGTCTTTCAGGAGTGCCAGGG
ATAAAAGGACCCAGAGGTGATCCGGGATGTCCAGGGGCTGAAGGGCCAGCTGGCATTCCT
GGATTCCTAGGTCTCAAAGGTCCCAAAGGCAGAGAGGGACATGCTGGGTTTCCAGGTGTC
CCAGGTCCACCTGGCCATTCCTGTGAAAGAGGTGCTCCAGGGATACCAGGGCAACCGGGA
CTCCCTGGGTATCCAGGTAGCCCAGGTGCTCCAGGTGGGAAAGGACAGCCGGGAGATGTG
GGGCCTCCCGGGCCAGCTGGAATGAAAGGCCTCCCCGGACTCCCAGGACGGCCTGGGGCA
CATGGTCCCCCAGGCCTCCCAGGAATCCCAGGTCCCTTTGGAGATGATGGGCTACCTGGT
CCTCCAGGTCCAAAGGGACCCCGGGGGCTGCCTGGTTTCCCAGGTTTTCCCGGAGAAAGA
GGAAAGCCTGGTGCAGAGGGATGTCCTGGCGCAAAGGGAGAACCTGGAGAGAAGGGCATG
TCTGGCCTTCCTGGAGACCGGGGACTGAGAGGGGCCAAAGGAGCCATAGGACCTCCCGGA
GATGAAGGAGAAATGGCTATCATTTCACAAAAGGGAACACCTGGGGAACCTGGACCTCCT
GGAGATGATGGATTCCCAGGAGAAAGAGGTGATAAAGGAACTCCCGGGATGCAAGGGAGA
AGAGGAGAGCCGGGAAGATACGGACCACCTGGATTTCACAGAGGGGAACCTGGTGAGAAA
GGTCAGCCAGGGCCTCCTGGACCCCCAGGCCCTCCAGGCTCAACTGGTCTAAGAGGGTTC
ATTGGTTTTCCAGGACTTCCAGGTGACCAGGGTGAGCCAGGTTCTCCAGGTCCCCCTGGA
TTTTCAGGAATTGATGGAGCAAGAGGACCTAAAGGAAACAAAGGTGACCCTGCCAGTCAC
TTTGGTCCACCTGGTCCAAAGGGTGAGCCAGGTAGCCCTGGATGTCCAGGGCATTTTGGA
GCATCCGGAGAGCAGGGCTTGCCTGGTATTCAAGGGCCCAGAGGATCACCTGGAAGGCCA
GGGCCACCTGGCTCCTCTGGACCACCAGGGTGCCCAGGTGATCACGGGATGCCTGGGCTG
AGGGGACAGCCAGGAGAAATGGGAGACCCTGGGCCAAGAGGCCTCCAGGGGGATCCAGGG
ATACCAGGTCCTCCGGGAATAAAAGGTCCCTCCGGATCACCTGGCCTGAACGGCTTGCAT
GGATTGAAAGGTCAGAAAGGAACTAAAGGTGCTTCAGGTTTGCATGATGTGGGGCCACCT
GGTCCAGTGGGAATACCTGGGCTAAAAGGGGAGAGAGGAGACCCTGGGAGCCCAGGAATC
TCTCCTCCAGGTCCTCGTGGAAAGAAAGGTCCCCCAGGACCCCCAGGGAGTTCAGGACCA
CCTGGTCCTGCAGGTGCCACAGGAAGAGCTCCTAAGGACATTCCTGACCCGGGTCCACCT
GGAGATCAGGGACCTCCTGGTCCTGATGGCCCAAGAGGAGCACCTGGGCCTCCAGGCCTC
CCTGGGAGTGTTGACCTTCTGAGAGGGGAGCCAGGTGACTGTGGTCTACCAGGGCCACCA
GGTCCCCCTGGCCCACCAGGCCCTCCAGGATACAAAGGCTTTCCAGGATGTGATGGAAAA
GATGGCCAGAAAGGACCAGTGGGATTCCCGGGACCGCAGGGACCACATGGATTTCCTGGG
CCACCTGGAGAGAAGGGTTTACCTGGACCTCCAGGGAGAAAAGGGCCCACTGGTCTTCCG
GGTCCCAGAGGTGAACCGGGGCCACCTGCAGATGTGGATGACTGTCCCCGAATCCCAGGC
CTTCCTGGGGCGCCAGGCATGAGAGGACCAGAAGGAGCCATGGGGCTCCCTGGAATGAGA
GGCCCCTCAGGACCAGGGTGCAAAGGAGAGCCTGGGCTGGATGGCAGGAGGGGTGTGGAT
GGCGTCCCTGGGTCTCCTGGGCCTCCCGGACGTAAAGGTGACACAGGAGAAGACGGCTAC
CCTGGAGGACCAGGGCCTCCTGGTCCCATTGGGGATCCTGGGCCCAAAGGGTTTGGCCCT
GGATACCTCGGTGGCTTCCTCCTGGTTCTCCACAGTCAGACGGACCAGGAGCCCACCTGC
CCCCTGGGCATGCCCAGGCTCTGGACTGGGTATAGTCTGTTATACCTGGAAGGGCAAGAG
AAAGCTCACAATCAAGACCTTGGTCTGGCAGGGTCTTGCCTTCCCGTATTTAGCACGCTG
CCCTTTGCCTACTGCAACATCCACCAGGTGTGCCACTATGCCCAGAGAAACGACAGATCC
TACTGGCTGGCCAGCGCTGCGCCCCTCCCCATGATGCCACTCTCTGAAGAGGCGATCCGC
CCCTATGTCAGCCGCTGTGCGGTATGCGAGGCCCCGGCCCAGGCGGTGGCGGTGCACAGC
CAGGACCAGTCCATCCCCCCATGTCCGCAGACCTGGAGGAGCCTCTGGATCGGGTATTCA
TTCCTGATGCACACAGGAGCTGGGGACCAAGGAGGAGGGCAGGCCCTTATGTCACCTGGC
AGCTGCCTGGAAGATTTCAGAGCAGCACCATTCCTTGAATGCCAGGGCCGGCAGGGAACT
TGCCACTTTTTCGCAAATAAGTATAGCTTCTGGCTCACAACGGTGAAAGCAGACTTGCAG
TTTTCCTCTGCTCCAGCACCAGACACCTTAAAAGAAAGCCAGGCCCAACGCCAGAAAATC
AGCCGGTGCCAGGTCTGCGTGAAGTATAGCTTG
Protein sequence predicted based on nucleotide sequence
MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPE KGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGL DGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILG AVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKG QMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGE KGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVT PPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPG EAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGR GPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGL PGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGP PGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKG QKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGP PGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPG IKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPG LPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPG PPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPG DEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEK GQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASH FGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGL RGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPP GPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPP GDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGK DGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPG LPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGY PGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQE KAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIR PYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPG SCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKI SRCQVCVKYSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
