Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859854
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 6012nts
Open reading frame: 1 to 6012
ATGCAGCCCCCTTCACTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTATGTGTCTCAGTG
GTCAGACCCAGAGGGCTGCTGTGTGGGAGTTTCCCAGAACCCTGTGCCAATGGAGGCACC
TGCCTGAGCCTGTCTCTGGGACAAGGGACCTGCCAGTGTGCCCCTGGCTTCCTGGGTGAG
ACGTGCCAGTTTCCTGACCCCTGCCAGAACGCCCAGCTCTGCCAAAATGGAGGCAGCTGC
CAAGCCCTGCTTCCCGCTCCCCTAGGGCTCCCCAGCTCTCCCTCTCCATTGACACCCAGC
TTCTTGTGCACTTGCCTCCCTGGCTTCACTGGTGAGAGATGCCAGGCCAAGCTTGAAGAC
CCTTGTCCTCCCTCCTTCTGTTCCAAAAGGGGCCGCTGCCACATCCAGGCCTCGGGCCGC
CCACAGTGCTCCTGCATGCCTGGATGGACAGGTGAGCAGTGCCAGCTTCGGGACTTCTGT
TCAGCCAACCCATGTGTTAATGGAGGGGTGTGTCTGGCCACATACCCCCAGATCCAGTGC
CACTGCCCACCGGGCTTCGAGGGCCATGCCTGTGAACGTGATGTCAACGAGTGCTTCCAG
GACCCAGGACCCTGCCCCAAAGGCACCTCCTGCCATAACACCCTGGGCTCCTTCCAGTGC
CTCTGCCCTGTGGGGCAGGAGGGTCCACGTTGTGAGCTGCGGGCAGGACCCTGCCCTCCT
AGGGGCTGTTCGAATGGGGGCACCTGCCAGCTGATGCCAGAGAAAGACTCCACCTTTCAC
CTCTGCCTCTGTCCCCCAGGTTTCATAGGCCCAGACTGTGAGGTGAATCCAGACAACTGT
GTCAGCCACCAGTGTCAGAATGGGGGCACTTGCCAGGATGGGCTGGACACCTACACCTGC
CTCTGCCCAGAAACCTGGACAGGCTGGGACTGCTCCGAAGATGTGGATGAGTGTGAGACC
CAGGGTCCCCCTCACTGCAGAAACGGGGGCACCTGCCAGAACTCTGCTGGTAGCTTTCAC
TGCGTGTGTGTGAGTGGCTGGGGCGGCACAAGCTGTGAGGAGAACCTGGATGACTGTATT
GCTGCCACCTGTGCCCCGGGATCCACCTGCATTGACCGGGTGGGCTCTTTCTCCTGCCTC
TGCCCACCTGGACGCACAGGACTCCTGTGCCACTTGGAAGACATGTGTCTGAGCCAGCCG
TGCCATGGGGATGCCCAATGCAGCACCAACCCCCTCACAGGCTCCACACTCTGCCTGTGT
CAGCCTGGCTATTCGGGGCCCACCTGCCACCAGGACCTGGACGAGTGTCTGATGGCCCAG
CAAGGCCCAAGTCCCTGTGAACATGGCGGTTCCTGCCTCAACACTCCTGGCTCCTTCAAC
TGCCTCTGTCCACCTGGCTACACAGGCTCCCGTTGTGAGGCTGATCACAATGAGTGCCTC
TCCCAGCCCTGCCACCCAGGAAGCACCTGTCTGGACCTACTTGCCACCTTCCACTGCCTC
TGCCCGCCAGGCTTAGAAGGGCAGCTCTGTGAGGTGGAGACCAACGAGTGTGCCTCAGCT
CCCTGCCTGAACCACGCGGATTGCCATGACCTGCTCAACGGCTTCCAGTGCATCTGCCTG
CCTGGATTCTCCGGCACCCGATGTGAGGAGGATATCGATGAGTGCAGAAGCTCTCCCTGT
GCCAATGGTGGGCAGTGCCAGGACCAGCCTGGAGCCTTCCACTGCAAGTGTCTCCCAGGC
TTTGAAGGGCCACGCTGTCAAACAGAGGTGGATGAGTGCCTGAGTGACCCATGTCCCGTT
GGAGCCAGCTGCCTTGATCTTCCAGGAGCCTTCTTTTGCCTCTGCCCCTCTGGTTTCACA
GGCCAGCTCTGTGAGGTTCCCCTGTGTGCTCCCAACCTGTGCCAGCCCAAGCAGATATGT
AAGGACCAGAAAGACAAGGCCAACTGCCTCTGTCCTGATGGAAGCCCTGGCTGTGCCCCA
CCTGAGGACAACTGCACCTGCCACCACGGGCACTGCCAGAGATCCTCATGTGTGTGTGAC
GTGGGTTGGACGGGGCCAGAGTGTGAGGCAGAGCTAGGGGGCTGCATCTCTGCACCCTGT
GCCCATGGGGGGACCTGCTACCCCCAGCCCTCTGGCTACAACTGCACCTGCCCTACAGGC
TACACAGGACCCACCTGTAGTGAGGAGATGACAGCTTGTCACTCAGGGCCATGTCTCAAT
GGCGGCTCCTGCAACCCTAGCCCTGGAGGCTACTACTGCACCTGCCCTCCAAGCCACACA
GGGCCCCAGTGCCAAACCAGCACTGACTACTGTGTGTCTGCCCCGTGCTTCAATGGGGGT
ACCTGTGTGAACAGGCCTGGCACCTTCTCCTGCCTCTGTGCCATGGGCTTCCAGGGCCCG
CGCTGTGAGGGAAAGCTCCGCCCCAGCTGTGCAGACAGCCCCTGTAGGAATAGGGCAACC
TGCCAGGACAGCCCTCAGGGTCCCCGCTGCCTCTGCCCCACTGGCTACACCGGAGGCAGC
TGCCAGACTCTGATGGACTTATGTGCCCAGAAGCCCTGCCCACGCAATTCCCACTGCCTC
CAGACTGGGCCCTCCTTCCACTGCTTGTGCCTCCAGGGATGGACCGGGCCTCTCTGCAAC
CTTCCACTGTCCTCCTGCCAGAAGGCTGCACTGAGCCAAGGCATAGACGTCTCTTCCCTT
TGCCACAATGGAGGCCTCTGTGTCGACAGCGGCCCCTCCTATTTCTGCCACTGCCCCCCT
GGATTCCAAGGCAGCCTGTGCCAGGATCACGTGAACCCATGTGAGTCCAGGCCTTGCCAG
AACGGGGCCACCTGCATGGCCCAGCCCAGTGGGTATCTCTGCCAGTGTGCCCCAGGCTAC
GATGGACAGAACTGCTCAAAGGAACTCGATGCTTGTCAGTCCCAACCCTGTCACAACCAT
GGAACCTGTACTCCCAAACCTGGAGGATTCCACTGTGCCTGCCCTCCAGGCTTTGTGGGG
CTACGCTGTGAGGGAGACGTGGACGAGTGTCTGGACCAGCCCTGCCACCCCACAGGCACT
GCAGCCTGCCACTCTCTGGCCAATGCCTTCTACTGCCAGTGTCTGCCTGGACACACAGGC
CAGTGGTGTGAGGTGGAGATAGACCCCTGCCACAGCCAACCCTGCTTTCATGGAGGGACC
TGTGAGGCCACAGCAGGATCACCCCTGGGTTTCATCTGCCACTGCCCCAAGGGTTTTGAA
GGCCCCACCTGCAGCCACAGGGCCCCTTCCTGCGGCTTCCATCACTGCCACCACGGAGGC
CTGTGTCTGCCCTCCCCTAAGCCAGGCTTCCCACCACGCTGTGCCTGCCTCAGTGGCTAT
GGGGGTCCTGACTGCCTGACCCCACCAGCTCCTAAAGGCTGTGGCCCTCCCTCCCCATGC
CTATACAATGGCAGCTGCTCAGAGACCACGGGCTTGGGGGGCCCAGGCTTTCGATGCTCC
TGCCCTCACAGCTCTCCAGGGCCCCGGTGTCAGAAACCCGGAGCCAAGGGGTGTGAGGGC
AGAAGTGGAGATGGGGCCTGCGATGCTGGCTGCAGTGGCCCGGGAGGAAACTGGGATGGA
GGGGACTGCTCTCTGGGAGTCCCAGACCCCTGGAAGGGCTGCCCCTCCCACTCTCGGTGC
TGGCTTCTCTTCCGGGACGGGCAGTGCCACCCACAGTGTGACTCTGAAGAGTGTCTGTTT
GATGGCTACGACTGTGAGACCCCTCCAGCCTGCACTCCAGCCTATGACCAGTACTGCCAT
GATCACTTCCACAACGGGCACTGTGAGAAAGGCTGCAACACTGCAGAGTGTGGCTGGGAT
GGAGGTGACTGCAGGCCTGAAGATGGGGACCCAGAGTGGGGGCCCTCCCTGGCCCTGCTG
GTGGTACTGAGCCCCCCAGCCCTAGACCAGCAGCTGTTTGCCCTGGCCCGGGTGCTGTCC
CTGACTCTGAGGGTAGGACTCTGGGTAAGGAAGGATCGTGATGGCAGGGACATGGTGTAC
CCCTATCCTGGGGCCCGGGCTGAAGAAAAGCTAGGAGGAACTCGGGACCCCACCTATCAG
GAGAGAGCAGCCCCTCAAACGCAGCCCCTGGGCAAGGAGACCGACTCCCTCAGTGCTGGG
TTTGTGGTGGTCATGGGTGTGGATTTGTCCCGCTGTGGCCCTGACCACCCGGCATCCCGC
TGTCCCTGGGACCCTGGGCTTCTACTCCGCTTCCTTGCTGCGATGGCTGCAGTGGGAGCC
CTGGAGCCCCTGCTGCCTGGACCACTGCTGGCTGTCCACCCTCATGCAGGGACCGCACCC
CCTGCCAACCAGCTTCCCTGGCCTGTGCTGTGCTCCCCAGTGGCCGGGGTGATTCTCCTG
GCCCTAGGGGCTCTTCTCGTCCTCCAGCTCATCCGGCGTCGACGCCGAGAGCATGGAGCT
CTCTGGCTGCCCCCTGGTTTCACTCGACGGCCTCGGACTCAGTCAGCTCCCCACCGACGC
CGGCCCCCACTAGGCGAGGACAGCATTGGTCTCAAGGCACTGAAGCCAAAGGCAGAAGTT
GATGAGGATGGAGTTGTGATGTGCTCAGGCCCTGAGGAGGGAGAGGAGGTGGGCCAGGCT
GAAGAAACAGGCCCACCCTCCACGTGCCAGCTCTGGTCTCTGAGTGGTGGCTGTGGGGCG
CTCCCTCAGGCAGCCATGCTAACTCCTCCCCAGGAATCTGAGATGGAAGCCCCTGACCTG
GACACCCGTGGACCTGATGGGGTGACACCCCTGATGTCAGCAGTTTGCTGTGGGGAAGTA
CAGTCCGGGACCTTCCAAGGGGCATGGTTGGGATGTCCTGAGCCCTGGGAACCTCTGCTG
GATGGAGGGGCCTGTCCCCAGGCTCACACCGTGGGCACTGGGGAGACCCCCCTGCACCTG
GCTGCCCGATTCTCCCGGCCAACCGCTGCCCGCCGCCTCCTTGAGGCTGGAGCCAACCCC
AACCAGCCAGACCGGGCAGGGCGCACACCCCTTCATGCTGCTGTGGCTGCTGATGCTCGG
GAGGTCTGCCAGCTTCTGCTCCGTAGCAGACAAACTGCAGTGGACGCTCGCACAGAGGAC
GGGACCACACCCTTGATGCTGGCTGCCAGGCTGGCGGTGGAAGACCTGGTTGAAGAACTG
ATTGCAGCCCAAGCAGACGTGGGGGCCAGAGATAAATGGGGGAAAACTGCGCTGCACTGG
GCTGCTGCCGTGAACAACGCCCGAGCCGCCCGCTCGCTTCTCCAGGCCGGAGCCGATAAA
GATGCCCAGGACAACAGGGAGCAGACGCCGCTATTCCTGGCGGCGCGGGAAGGAGCGGTG
GAAGTAGCCCAGCTACTGCTGGGGCTGGGGGCAGCCCGAGAGCTGCGGGACCAGGCTGGG
CTAGCGCCGGCGGACGTCGCTCACCAACGTAACCACTGGGATCTGCTGACGCTGCTGGAA
GGGGCTGGGCCACCAGAGGCCCGTCACAAAGCCACGCCGGGCCGCGAGGCTGGGCCCTTC
CCGCGCGCACGGACGGTGTCAGTAAGCGTGCCCCCGCATGGGGGCGGGGCTCTGCCGCGC
TGCCGGACGCTGTCAGCCGGAGCAGGCCCTCGTGGGGGCGGAGCTTGTCTGCAGGCTCGG
ACTTGGTCCGTAGACTTGGCTGCGCGGGGGGGCGGGGCCTATTCTCATTGCCGGAGCCTC
TCGGGAGTAGGAGCAGGAGGAGGCCCGACCCCTCGCGGCCGTAGGTTTTCTGCAGGCATG
CGCGGGCCTCGGCCCAACCCTGCGATAATGCGAGGAAGATACGGAGTGGCTGCCGGGCGC
GGAGGCAGGGTCTCAACGGATGACTGGCCCTGTGATTGGGTGGCCCTGGGAGCTTGCGGT
TCTGCCTCCAACATTCCGATCCCGCCTCCTTGCCTTACTCCGTCCCCGGAGCGGGGATCA
CCTCAACTTGACTGTGGTCCCCCAGCCCTCCAAGAAATGCCCATAAACCAAGGAGGAGAG
GGTAAAAAATTG
Protein sequence predicted based on nucleotide sequence
MQPPSLLLLLLLLLLLCVSVVRPRGLLCGSFPEPCANGGTCLSLSLGQGTCQCAPGFLGE TCQFPDPCQNAQLCQNGGSCQALLPAPLGLPSSPSPLTPSFLCTCLPGFTGERCQAKLED PCPPSFCSKRGRCHIQASGRPQCSCMPGWTGEQCQLRDFCSANPCVNGGVCLATYPQIQC HCPPGFEGHACERDVNECFQDPGPCPKGTSCHNTLGSFQCLCPVGQEGPRCELRAGPCPP RGCSNGGTCQLMPEKDSTFHLCLCPPGFIGPDCEVNPDNCVSHQCQNGGTCQDGLDTYTC LCPETWTGWDCSEDVDECETQGPPHCRNGGTCQNSAGSFHCVCVSGWGGTSCEENLDDCI AATCAPGSTCIDRVGSFSCLCPPGRTGLLCHLEDMCLSQPCHGDAQCSTNPLTGSTLCLC QPGYSGPTCHQDLDECLMAQQGPSPCEHGGSCLNTPGSFNCLCPPGYTGSRCEADHNECL SQPCHPGSTCLDLLATFHCLCPPGLEGQLCEVETNECASAPCLNHADCHDLLNGFQCICL PGFSGTRCEEDIDECRSSPCANGGQCQDQPGAFHCKCLPGFEGPRCQTEVDECLSDPCPV GASCLDLPGAFFCLCPSGFTGQLCEVPLCAPNLCQPKQICKDQKDKANCLCPDGSPGCAP PEDNCTCHHGHCQRSSCVCDVGWTGPECEAELGGCISAPCAHGGTCYPQPSGYNCTCPTG YTGPTCSEEMTACHSGPCLNGGSCNPSPGGYYCTCPPSHTGPQCQTSTDYCVSAPCFNGG TCVNRPGTFSCLCAMGFQGPRCEGKLRPSCADSPCRNRATCQDSPQGPRCLCPTGYTGGS CQTLMDLCAQKPCPRNSHCLQTGPSFHCLCLQGWTGPLCNLPLSSCQKAALSQGIDVSSL CHNGGLCVDSGPSYFCHCPPGFQGSLCQDHVNPCESRPCQNGATCMAQPSGYLCQCAPGY DGQNCSKELDACQSQPCHNHGTCTPKPGGFHCACPPGFVGLRCEGDVDECLDQPCHPTGT AACHSLANAFYCQCLPGHTGQWCEVEIDPCHSQPCFHGGTCEATAGSPLGFICHCPKGFE GPTCSHRAPSCGFHHCHHGGLCLPSPKPGFPPRCACLSGYGGPDCLTPPAPKGCGPPSPC LYNGSCSETTGLGGPGFRCSCPHSSPGPRCQKPGAKGCEGRSGDGACDAGCSGPGGNWDG GDCSLGVPDPWKGCPSHSRCWLLFRDGQCHPQCDSEECLFDGYDCETPPACTPAYDQYCH DHFHNGHCEKGCNTAECGWDGGDCRPEDGDPEWGPSLALLVVLSPPALDQQLFALARVLS LTLRVGLWVRKDRDGRDMVYPYPGARAEEKLGGTRDPTYQERAAPQTQPLGKETDSLSAG FVVVMGVDLSRCGPDHPASRCPWDPGLLLRFLAAMAAVGALEPLLPGPLLAVHPHAGTAP PANQLPWPVLCSPVAGVILLALGALLVLQLIRRRRREHGALWLPPGFTRRPRTQSAPHRR RPPLGEDSIGLKALKPKAEVDEDGVVMCSGPEEGEEVGQAEETGPPSTCQLWSLSGGCGA LPQAAMLTPPQESEMEAPDLDTRGPDGVTPLMSAVCCGEVQSGTFQGAWLGCPEPWEPLL DGGACPQAHTVGTGETPLHLAARFSRPTAARRLLEAGANPNQPDRAGRTPLHAAVAADAR EVCQLLLRSRQTAVDARTEDGTTPLMLAARLAVEDLVEELIAAQADVGARDKWGKTALHW AAAVNNARAARSLLQAGADKDAQDNREQTPLFLAAREGAVEVAQLLLGLGAARELRDQAG LAPADVAHQRNHWDLLTLLEGAGPPEARHKATPGREAGPFPRARTVSVSVPPHGGGALPR CRTLSAGAGPRGGGACLQARTWSVDLAARGGGAYSHCRSLSGVGAGGGPTPRGRRFSAGM RGPRPNPAIMRGRYGVAAGRGGRVSTDDWPCDWVALGACGSASNIPIPPPCLTPSPERGS PQLDCGPPALQEMPINQGGEGKKL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
