Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859875
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3996nts
Open reading frame: 1 to 3996
ATGCAGGCGGCTCCGCGCGCCGGCTGCGGGGCAGCGCTCCTGCTGTGGATTGTCAGCAGC
TGCCTCTGCAGAGCCTGGACGGCTCCCTCCACGTCCCAAAAATGTGATGAGCCACTTGTC
TCTGGACTCCCCCATGTGGCTTTCAGCAGCTCCTCCTCCATCTCTGGTAGCTATTCTCCC
GGCTATGCCAAGATAAACAAGAGAGGAGGTGCTGGGGGATGGTCTCCATCAGACAGCGAC
CATTATCAATGGCTTCAGGTTGACTTTGGCAATCGGAAGCAGATCAGTGCCATTGCAACC
CAAGGAAGGTATAGCAGCTCAGATTGGGTGACCCAATACCGGATGCTCTACAGCGACACA
GGGAGAAACTGGAAACCCTATCATCAAGATGGGAATATCTGGGCATTTCCCGGAAACATT
AACTCTGACGGTGTGGTCCGGCACGAATTACAGCATCCGATTATTGCCCGCTATGTGCGC
ATAGTGCCTCTGGATTGGAATGGAGAAGGTCGCATTGGACTCAGAATTGAAGTTTATGGC
TGTTCTTACTGGGCTGATGTTATCAACTTTGATGGCCATGTTGTATTACCATATAGATTC
AGAAACAAGAAGATGAAAACACTGAAAGATGTCATTGCCTTGAACTTTAAGACGTCTGAA
AGTGAAGGAGTAATCCTGCACGGAGAAGGACAGCAAGGAGATTACATTACCTTGGAACTG
AAAAAAGCCAAGCTGGTCCTCAGTTTAAACTTAGGAAGCAACCAGCTTGGCCCCATATAT
GGCCACACATCAGTGATGACAGGAAGTTTGCTGGATGACCACCACTGGCACTCTGTGGTC
ATTGAGCGCCAGGGGCGGAGCATTAACCTCACTCTGGACAGGAGCATGCAGCACTTCCGT
ACCAATGGAGAGTTTGACTACCTGGACTTGGACTATGAGATAACCTTTGGAGGCATCCCT
TTCTCTGGCAAGCCCAGCTCCAGCAGTAGAAAGAATTTCAAAGGCTGCATGGAAAGCATC
AACTACAATGGCGTCAACATTACTGATCTTGCCAGAAGGAAGAAATTAGAGCCCTCAAAT
GTGGGAAATTTGAGCTTTTCTTGTGTGGAACCCTATACGGTGCCTGTCTTTTTCAACGCT
ACAAGTTACCTGGAGGTGCCCGGACGGCTTAACCAGGACCTGTTCTCAGTCAGTTTCCAG
TTTAGGACATGGAACCCCAATGGTCTCCTGGTCTTCAGTCACTTTGCGGATAATTTGGGC
AATGTGGAGATTGACCTCACTGAAAGCAAAGTGGGTGTTCACATCAACATCACACAGACC
AAGATGAGCCAAATCGATATTTCCTCAGGTTCTGGGTTGAATGATGGACAGTGGCACGAG
GTTCGCTTCCTAGCCAAGGAAAATTTTGCTATTCTCACCATCGATGGAGATGAAGCATCA
GCAGTTCGAACTAATAGTCCCCTTCAAGTTAAAACTGGCGAGAAGTACTTTTTTGGAGGT
TTTCTGAACCAGATGAATAACTCAAGTCACTCTGTCCTTCAGCCTTCATTCCAAGGATGC
ATGCAGCTCATTCAAGTGGACGATCAACTTGTAAATTTATACGAAGTGGCACAAAGGAAG
CCGGGAAGTTTCGCGAATGTCAGCATTGACATGTGTGCGATCATAGACAGATGTGTGCCC
AATCACTGTGAGCATGGTGGAAAGTGCTCGCAAACATGGGACAGCTTCAAATGCACTTGT
GATGAGACAGGATACAGTGGGGCCACCTGCCACAACTCTATCTACGAGCCTTCCTGTGAA
GCCTACAAACACCTAGGACAGACATCAAATTATTACTGGATAGATCCTGATGGCAGCGGA
CCTCTGGGGCCTCTGAAAGTTTACTGCAACATGACAGAGGACAAAGTGTGGACCATAGTG
TCTCATGACTTGCAGATGCAGACGCCTGTGGTCGGCTACAACCCAGAAAAATACTCAGTG
ACACAGCTCGTTTACAGCGCCTCCATGGACCAGATAAGTGCCATCACTGACAGTGCCGAG
TACTGCGAGCAGTATGTCTCCTATTTCTGCAAGATGTCAAGATTGTTGAACACCCCAGAT
GGAAGCCCTTACACTTGGTGGGTTGGCAAAGCCAACGAGAAGCACTACTACTGGGGAGGC
TCTGGGCCTGGAATCCAGAAATGTGCCTGCGGCATCGAACGCAACTGCACAGATCCCAAG
TACTACTGTAACTGCGACGCGGACTACAAGCAATGGAGGAAGGATGCTGGTTTCTTATCA
TACAAAGATCACCTGCCAGTGAGCCAAGTGGTGGTTGGAGATACTGACCGTCAAGGCTCA
GAAGCCAAATTGAGCGTAGGTCCTCTGCGCTGCCAAGGAGACAGGAATTATTGGAATGCC
GCCTCTTTCCCAAACCCATCCTCCTACCTGCACTTCTCTACTTTCCAAGGGGAAACTAGC
GCTGACATTTCTTTCTACTTCAAAACATTAACCCCCTGGGGAGTGTTTCTTGAAAATATG
GGAAAGGAAGATTTCATCAAGCTGGAGCTGAAGTCTGCCACAGAAGTGTCCTTTTCATTT
GATGTGGGAAATGGGCCAGTAGAGATTGTAGTGAGGTCACCAACCCCTCTCAACGATGAC
CAGTGGCACCGGGTCACTGCAGAGAGGAATGTCAAGCAGGCCAGCCTACAGGTGGACCGG
CTACCGCAGCAGATCCGCAAGGCCCCAACAGAAGGCCACACCCGCCTGGAGCTCTACAGC
CAGTTATTTGTGGGTGGTGCTGGGGGCCAGCAGGGCTTCCTGGGCTGCATCCGCTCCTTG
AGGATGAATGGGGTGACACTTGACCTGGAGGAAAGAGCAAAGGTCACATCTGGGTTCATA
TCCGGATGCTCGGGCCATTGCACCAGCTATGGAACAAACTGTGAAAATGGAGGCAAATGC
CTAGAGAGATACCACGGTTACTCCTGCGATTGCTCTAATACTGCATATGATGGAACATTT
TGCAACAAAGATGTTGGTGCATTTTTTGAAGAAGGGATGTGGCTACGATATAACTTTCAG
GCACCAGCAACAAATGCCAGAGACTCCAGCAGCAGAGTAGACAACGCTCCCGACCAGCAG
AACTCCCACCCGGACCTGGCACAGGAGGAGATCCGCTTCAGCTTCAGCACCACCAAGGCG
CCCTGCATTCTCCTCTACATCAGCTCCTTCACCACAGACTTCTTGGCAGTCCTCGTCAAA
CCCACTGGAAGCTTACAGATTCGATACAACCTGGGTGGCACCCGAGAGCCATACAATATT
GACGTAGACCACAGGAACATGGCCAATGGACAGCCCCACAGTGTCAACATCACCCGCCAC
GAGAAGACCATCTTTCTCAAGCTCGATCATTATCCTTCTGTGAGTTACCATCTGCCAAGT
TCATCCGACACCCTCTTCAATTCTCCCAAGTCGCTCTTTCTGGGAAAAGTTATAGAAACA
GGGAAAATTGACCAAGAGATTCACAAATACAACACCCCAGGATTCACTGGTTGCCTCTCC
AGAGTCCAGTTCAACCAGATCGCCCCTCTCAAGGCCGCCTTGAGGCAGACAAACGCCTCG
GCTCACGTCCACATCCAGGGCGAGCTGGTGGAGTCCAACTGCGGGGCCTCGCCGCTGACC
CTCTCCCCCATGTCGTCCGCCACCGACCCCTGGCACCTGGATCACCTGGATTCAGCCAGT
GCGGATTTTCCATATAATCCAGGACAAGGCCAAGCTATAAGAAATGGAGTCAACAGAAAC
TCGGCTATCATTGGAGGCGTCATTGCTGTGGTGATTTTCACCATCCTGTGCACCCTGGTC
TTCCTGATCCGGTACATGTTCCGCCACAAGGGCACCTACCATACCAACGAAGCAAAGGGG
GCGGAGTCGGCAGAGAGCGCGGACGCCGCCATCATGAACAACGACCCCAACTTCACAGAG
ACCATTGATGAAAGCAAAAAGGAATGGCTCATTTTG
Protein sequence predicted based on nucleotide sequence
MQAAPRAGCGAALLLWIVSSCLCRAWTAPSTSQKCDEPLVSGLPHVAFSSSSSISGSYSP GYAKINKRGGAGGWSPSDSDHYQWLQVDFGNRKQISAIATQGRYSSSDWVTQYRMLYSDT GRNWKPYHQDGNIWAFPGNINSDGVVRHELQHPIIARYVRIVPLDWNGEGRIGLRIEVYG CSYWADVINFDGHVVLPYRFRNKKMKTLKDVIALNFKTSESEGVILHGEGQQGDYITLEL KKAKLVLSLNLGSNQLGPIYGHTSVMTGSLLDDHHWHSVVIERQGRSINLTLDRSMQHFR TNGEFDYLDLDYEITFGGIPFSGKPSSSSRKNFKGCMESINYNGVNITDLARRKKLEPSN VGNLSFSCVEPYTVPVFFNATSYLEVPGRLNQDLFSVSFQFRTWNPNGLLVFSHFADNLG NVEIDLTESKVGVHINITQTKMSQIDISSGSGLNDGQWHEVRFLAKENFAILTIDGDEAS AVRTNSPLQVKTGEKYFFGGFLNQMNNSSHSVLQPSFQGCMQLIQVDDQLVNLYEVAQRK PGSFANVSIDMCAIIDRCVPNHCEHGGKCSQTWDSFKCTCDETGYSGATCHNSIYEPSCE AYKHLGQTSNYYWIDPDGSGPLGPLKVYCNMTEDKVWTIVSHDLQMQTPVVGYNPEKYSV TQLVYSASMDQISAITDSAEYCEQYVSYFCKMSRLLNTPDGSPYTWWVGKANEKHYYWGG SGPGIQKCACGIERNCTDPKYYCNCDADYKQWRKDAGFLSYKDHLPVSQVVVGDTDRQGS EAKLSVGPLRCQGDRNYWNAASFPNPSSYLHFSTFQGETSADISFYFKTLTPWGVFLENM GKEDFIKLELKSATEVSFSFDVGNGPVEIVVRSPTPLNDDQWHRVTAERNVKQASLQVDR LPQQIRKAPTEGHTRLELYSQLFVGGAGGQQGFLGCIRSLRMNGVTLDLEERAKVTSGFI SGCSGHCTSYGTNCENGGKCLERYHGYSCDCSNTAYDGTFCNKDVGAFFEEGMWLRYNFQ APATNARDSSSRVDNAPDQQNSHPDLAQEEIRFSFSTTKAPCILLYISSFTTDFLAVLVK PTGSLQIRYNLGGTREPYNIDVDHRNMANGQPHSVNITRHEKTIFLKLDHYPSVSYHLPS SSDTLFNSPKSLFLGKVIETGKIDQEIHKYNTPGFTGCLSRVQFNQIAPLKAALRQTNAS AHVHIQGELVESNCGASPLTLSPMSSATDPWHLDHLDSASADFPYNPGQGQAIRNGVNRN SAIIGGVIAVVIFTILCTLVFLIRYMFRHKGTYHTNEAKGAESAESADAAIMNNDPNFTE TIDESKKEWLIL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
