Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859884
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 6408nts
Open reading frame: 1 to 6408
ATGCCTGCTCTGGGCCCAGCTCTTCTCCAGGCTCTCTGGGCCGGGTGGGTCCTCACCCTC
CAGCCCCTTCCACCAACTGCATTCACTCCCAATGGCACGTATCTGCAGCACCTGGCAAGG
GACCCCACCTCAGGCACCCTCTACCTGGGGGCTACCAACTTCCTGTTCCAGCTGAGCCCT
GGGCTGCAGCTGGAGGCCACAGTGTCCACCGGCCCTGTGCTAGACAGCAGGGACTGCCTG
CCACCTGTGATGCCTGATGAGTGCCCCCAGGCCCAGCCTACCAACAACCCGAATCAGCTG
CTCCTGGTGAGCCCAGGGGCCCTGGTGGTATGCGGGAGCGTGCACCAGGGGGTCTGTGAA
CAGCGGCGCCTGGGGCAGCTCGAGCAGCTGCTGCTGCGGCCAGAGCGGCCTGGGGACACA
CAATATGTGGCTGCCAATGATCCTGCGGTCAGCACGGTGGGGCTGGTAGCCCAGGGCTTG
GCAGGGGAGCCCCTCCTGTTTGTGGGGCGAGGATACACCAGCAGGGGTGTGGGGGGTGGC
ATTCCACCCATCACAACCCGGGCCCTGTGGCCGCCCGACCCCCAAGCTGCCTTCTCCTAT
GAGGAGACAGCCAAGCTGGCAGTGGGCCGCCTCTCCGAGTACAGCCACCACTTCGTGAGT
GCCTTTGCACGTGGGGCCAGCGCCTACTTCCTGTTCCTGCGGCGGGACCTGCAGGCTCAG
TCTAGAGCTTTTCGTGCCTATGTATCTCGAGTGTGTCTCCGGGACCAGCACTACTACTCC
TATGTGGAGTTGCCTCTGGCCTGCGAAGGTGGCCGCTACGGGCTGATCCAGGCTGCAGCT
GTGGCCACGTCCAGGGAGGTGGCGCATGGGGAGGTGCTCTTTGCAGCTTTCTCCTCGGCT
GCACCCCCCACTGTGGGCCGGCCCCCATCGGCGGCTGCTGGGGCATCTGGAGCCTCTGCC
CTCTGTGCCTTCCCCCTGGATGAGGTGGACCGGCTTGCTAATCGCACGCGAGATGCCTGC
TACACCCGGGAGGGTCGTGCTGAGGATGGGACCGAGGTGGCCTACATCGAGTATGATGTC
AATTCTGACTGTGCACAGCTGCCAGTGGACACCCTGGATGCTTATCCCTGTGGCTCAGAC
CACACGCCCAGCCCCATGGCCAGCCGGGTCCCGCTGGAAGCCACACCAATTCTGGAGTGG
CCAGGGATTCAGCTAACAGCTGTGGCAGTCACCATGGAAGATGGACACACCATCGCTTTC
CTGGGTGATAGTCAAGGGCAGCTGCACAGGGTCTACTTGGGCCCAGGGAGCGATGGCCAC
CCATACTCCACACAGAGCATCCAGCAGGGGTCTGCAGTGAGCAGAGACCTCACCTTTGAT
GGGACCTTTGAGCACCTGTATGTCATGACCCAGAGCACACTTCTGAAGGTTCCTGTGGCT
TCCTGTGCTCAGCACCTGGACTGTGCATCTTGCCTTGCTCACAGGGACCCATACTGTGGG
TGGTGCGTGCTCCTTGGCAGGTGCAGTCGCCGTTCTGAGTGCTCGAGGGGCCAGGGCCCA
GAGCAGTGGCTATGGAGCTTCCAGCCTGAGCTGGGCTGTCTGCAAGTGGCAGCCATGAGT
CCTGCCAACATCAGCCGAGAGGAGACGAGGGAGGTTTTCCTATCAGTGCCAGACCTGCCA
CCCCTGTGGCCAGGGGAGTCATATTCCTGCCACTTTGGGGAACATCAGAGTCCTGCCCTG
CTGACTGGTTCTGGTGTGATGTGCCCCTCCCCAGACCCTAGTGAGGCCCCAGTGCTGCCG
AGAGGAGCCGACTACGTATCCGTGAGCGTGGAGCTCAGATTTGGCGCTGTTGTGATCGCC
AAAACTTCCCTCTCTTTCTATGACTGTGTGGCGGTCACTGAACTCCGCCCATCTGCGCAG
TGCCAGGCCTGTGTGAGCAGCCGCTGGGGGTGTAACTGGTGTGTCTGGCAGCACCTGTGC
ACCCACAAGGCCTCGTGTGATGCTGGGCCCATGGTTGCAAGCCATCAGAGCCCGCTTGTC
TCCCCAGACCCTCCTGCAAGAGGTGGACCCAGCCCCTCCCCACCCACAGCCCCCAAAGCC
CTGGCCACCCCTGCTCCTGACACCCTTCCCGTGGAGCCTGGGGCTCCCTCCACAGCCACA
GCTTCGGACATCTCACCTGGGGCTAGTCCTTCCCTGCTCAGCCCCTGGGGGCCATGGGCA
GGTTCTGGCTCCATATCTTCCCCTGGCTCCACAGGGTCGCCTCTCCATGAGGAGCCCTCC
CCTCCCAGCCCCCAAAATGGACCTGGAACCGCTGTCCCTGCCCCCACTGACTTCAGACCC
TCAGCCACACCTGAGGACCTCTTGGCCTCCCCGCTGTCACCCTCAGAGGTAGCAGCAGTG
CCCCCTGCAGACCCTGGCCCCGAGGCTCTTCATCCCACAGTGCCCCTGGACCTGCCCCCT
GCCACTGTTCCTGCCACCACTTTCCCAGGGGCCATGGGCTCCGTGAAGCCCGCCCTGGAC
TGGCTCACGAGAGAAGGCGGCGAGCTGCCCGAGGCGGACGAGTGGACGGGGGGTGACGCA
CCCGCCTTCTCCACTTCCACCCTCCTCTCAGGTGATGGAGACTCAGCAGAGCTTGAGGGC
CCTCCCGCCCCCCTCATCCTCCCGTCCAGCCTCGACTACCAGTATGACACCCCCGGGCTC
TGGGAGCTGGAAGAGGCGACCTTGGGGGCAAGCTCCTGCCCCTGTGTGGAGAGCGTTCAG
GGCTCCACGTTGATGCCGGTCCATGTGGAGCGGGAAATCCGGCTGCTAGGCAGGAACCTG
CACCTTTTCCAGGATGGCCCAGGAGACAATGAGTGTGTGATGGAGCTGGAGGGCCTCGAG
GTGGTGGTTGAGGCCCGGGTCGAGTGTGAGCCACCTCCAGATACCCAGTGCCATGTCACC
TGCCAGCAGCACCAGCTCAGCTATGAGGCTCTGCAGCCGGAGCTCCGTGTGGGGCTGTTT
CTGCGTCGGGCCGGCCGTCTGCGTGTGGACAGTGCTGAGGGGCTGCATGTGGTACTGTAT
GACTGTTCCGTGGGACATGGAGACTGCAGCCGCTGCCAAACTGCCATGCCCCAGTATGGC
TGTGTGTGGTGTGAGGGGGAGCGTCCACGTTGTGTGACCCGGGAGGCCTGTGGTGAGGCT
GAGGCTGTGGCCACCCAGTGCCCAGCGCCCCTCATCCACTCGGTGGAGCCACTGACTGGG
CCTGTAGACGGAGGCACCCGTGTCACCATCAGGGGCTCCAACCTGGGCCAGCATGTGCAG
GATGTGCTGGGCATGGTCACGGTGGCTGGAGTGCCCTGTGCTGTGGATGCCCAGGAGTAC
GAGGTCTCCAGCAGCCTCGTGTGCATCACCGGGGCCAGTGGGGAGGAGGTGGCCGGCGCC
ACAGCGGTGGAGGTGCCGGGAAGAGGACGTGGTGTCTCAGAACACGACTTTGCCTACCAG
GATCCGAAGGTCCATTCCATCTTCCCGGCCCGCGGCCCCAGAGCTGGGGGCACCCGTCTC
ACCCTGAATGGCTCCAAGCTCCTGACTGGGCGGCTGGAGGACATCCGAGTGGTGGTTGGA
GACCAGCCTTGTCACTTGCTGCCGGAGCAGCAGTCAGAACAACTGCGGTGTGAGACCAGC
CCACGCCCCACGCCTGCCACGCTCCCTGTGGCTGTGTGGTTTGGGGCCACGGAGCGGAGG
CTTCAACGCGGACAGTTCAAGTATACCTTGGACCCCAACATCACCTCTGCTGGCCCCACC
AAGAGCTTCCTCAGTGGAGGACGTGAGATATGCGTCCGTGGCCAGAATCTGGACGTGGTA
CAGACGCCAAGAATCCGGGTGACCGTGGTCTCGAGAATGCTGCAGCCCAGCCAGGGGCTT
GGACGGAGGCGTCGCGTGGTCCCGGAGACGGCATGTTCCCTTGGACCCTCCTGCAGTAGC
CAGCAATTTGAGGAGCCGTGCCATGTCAACTCCTCCCAGCTCATCACGTGCCGCACACCT
GCCCTCCCAGGCCTGCCTGAGGACCCCTGGGTCCGGGTGGAATTTATCCTTGACAACCTG
GTCTTTGACTTTGCAACACTGAACCCCACACCTTTCTCCTATGAGGCCGACCCCACCCTG
CAGCCACTCAACCCTGAGGACCCCACCATGCCATTCCGGCACAAGCCTGGGAGTGTGTTC
TCCGTGGAGGGGGAGAACCTGGACCTTGCAATGTCCAAGGAGGAGGTGGTGGCTATGATA
GGGGATGGCCCCTGTGTGGTGAAGACGCTGACGCGGCACCACCTGTACTGCGAGCCCCCC
GTGGAGCAGCCCCTGCCACGGCACCATGCCCTCCGAGAGGCACCTGACTCTTTGCCTGAG
TTCACGGTGCAGATGGGGAACTTGCGCTTCTCCCTGGGTCACGTGCAGTATGACGGCGAG
AGCCCTGGGGCTTTTCCTGTGGCAGCCCAGGTGGGCTTGGGGGTGGGCACCTCTCTTCTG
GCTCTGGGTGTCATCATCATTGTCCTCATGTACAGGAGGAAGAGCAAGCAGGCCCTGAGG
GACTATAAGAAGGTTCAGATCCAGCTGGAGAATCTGGAGAGCAGTGTGCGGGACCGCTGC
AAGAAGGAATTCACAGACCTCATGACTGAGATGACCGATCTCACCAGTGACCTCCTGGGC
AGCGGCATCCCCTTCCTCGACTACAAGGTGTATGCGGAGAGGATCTTCTTCCCTGGGCAC
CGCGAGTCGCCCTTGCACCGGGACCTGGGTGTGCCTGAGAGCAGACGGCCCACTGTGGAG
CAAGGGCTGGGGCAGCTCTCTAACCTGCTCAACAGCAAGCTCTTCCTCACCAAGTTCATC
CACACGCTGGAGAGCCAGCGCACCTTTTCAGCTCGGGACCGTGCCTACGTGGCATCTCTG
CTCACCGTGGCACTGCATGGGAAGCTTGAGTATTTCACTGACATCCTCCGCACTCTGCTC
AGTGACCTGGTTGCCCAGTATGTGGCCAAGAACCCCAAGCTGATGCTGCGCAGGACAGAG
ACTGTGGTGGAGAAGCTGCTCACCAACTGGATGTCCATCTGTCTGTATACCTTCGTGAGG
GACTCCGTAGGGGAGCCTCTGTACATGCTCTTTCGAGGGATTAAGCACCAAGTGGATAAG
GGGCCAGTGGACAGTGTGACAGGCAAGGCCAAATACACCTTGAACGACAACCGCCTGCTC
AGAGAGGATGTGGAGTACCGTCCCCTGACCTTGAATGCACTATTGGCTGTGGGGCCTGGG
GCAGGAGAGGCCCAGGGCGTGCCCGTGAAGGTCCTAGACTGTGACACCATCTCCCAGGCA
AAGGAGAAGATGCTGGACCAGCTTTATAAAGGAGTGCCTCTCACCCAGCGGCCAGACCCT
CGCACCCTTGATGTTGAGTGGCGGTCTGGGGTGGCCGGGCACCTCATTCTTTCTGACGAG
GATGTCACTTCTGAGGTCCAGGGTCTGTGGAGGCGCCTGAACACACTGCAGCATTACAAG
GTCCCAGATGGAGCAACTGTGGCCCTCGTCCCCTGCCTCACCAAGCATGTGCTCCGGGAA
AACCAGGATTATGTCCCTGGAGAGCGGACCCCAATGCTGGAGGATGTAGATGAGGGGGGC
ATCCGGCCCTGGCACCTGGTGAAGCCAAGTGATGAGCCGGAGCCGCCCAGGCCTCGGAGG
GGCAGCCTTCGGGGCGGGGAGCGTGAGCGCGCCAAGGCCATCCCTGAGATCTACCTGACC
CGCCTGCTGTCCATGAAGGGCACCCTGCAGAAGTTCGTGGATGACCTGTTCCAGGTGATT
CTCAGCACCAGCCGCCCCGTGCCGCTCGCTGTGAAGTACTTCTTTGACCTGCTGGATGAG
CAGGCCCAGCAGCATGGCATCTCCGACCAGGACACCATCCACATCTGGAAGACCAACAGC
TTGCCTCTGAGGTTCTGGATCAATATAATAAAAAACCCGCAGTTTGTGTTCGACGTGCAA
ACATCTGATAACATGGATGCGGTGCTCCTTGTCATTGCACAGACCTTCATGGACGCCTGC
ACCCTGGCCGACCACAAGCTGGGCCGGGACTCCCCGATCAACAAACTTCTGTATGCACGG
GACATTCCCCGGTACAAGCGGATGGTGGAAAGGTACTATGCAGACATCAGACAGACTGTC
CCAGCCAGCGACCAAGAGATGAACTCTGTCCTGGCTGAACTGTCCTGGAACTACTCCGGA
GACCTCGGGGCGCGAGTGGCCCTGCATGAACTCTACAAGTACATCAACAAGTACTATGAC
CAGATCATCACTGCCCTGGAGGAGGATGGCACGGCCCAGAAGATGCAGCTGGGCTATCGG
CTCCAGCAGATTGCAGCTGCTGTGGAAAACAAGGTCACAGATCTATTG
Protein sequence predicted based on nucleotide sequence
MPALGPALLQALWAGWVLTLQPLPPTAFTPNGTYLQHLARDPTSGTLYLGATNFLFQLSP GLQLEATVSTGPVLDSRDCLPPVMPDECPQAQPTNNPNQLLLVSPGALVVCGSVHQGVCE QRRLGQLEQLLLRPERPGDTQYVAANDPAVSTVGLVAQGLAGEPLLFVGRGYTSRGVGGG IPPITTRALWPPDPQAAFSYEETAKLAVGRLSEYSHHFVSAFARGASAYFLFLRRDLQAQ SRAFRAYVSRVCLRDQHYYSYVELPLACEGGRYGLIQAAAVATSREVAHGEVLFAAFSSA APPTVGRPPSAAAGASGASALCAFPLDEVDRLANRTRDACYTREGRAEDGTEVAYIEYDV NSDCAQLPVDTLDAYPCGSDHTPSPMASRVPLEATPILEWPGIQLTAVAVTMEDGHTIAF LGDSQGQLHRVYLGPGSDGHPYSTQSIQQGSAVSRDLTFDGTFEHLYVMTQSTLLKVPVA SCAQHLDCASCLAHRDPYCGWCVLLGRCSRRSECSRGQGPEQWLWSFQPELGCLQVAAMS PANISREETREVFLSVPDLPPLWPGESYSCHFGEHQSPALLTGSGVMCPSPDPSEAPVLP RGADYVSVSVELRFGAVVIAKTSLSFYDCVAVTELRPSAQCQACVSSRWGCNWCVWQHLC THKASCDAGPMVASHQSPLVSPDPPARGGPSPSPPTAPKALATPAPDTLPVEPGAPSTAT ASDISPGASPSLLSPWGPWAGSGSISSPGSTGSPLHEEPSPPSPQNGPGTAVPAPTDFRP SATPEDLLASPLSPSEVAAVPPADPGPEALHPTVPLDLPPATVPATTFPGAMGSVKPALD WLTREGGELPEADEWTGGDAPAFSTSTLLSGDGDSAELEGPPAPLILPSSLDYQYDTPGL WELEEATLGASSCPCVESVQGSTLMPVHVEREIRLLGRNLHLFQDGPGDNECVMELEGLE VVVEARVECEPPPDTQCHVTCQQHQLSYEALQPELRVGLFLRRAGRLRVDSAEGLHVVLY DCSVGHGDCSRCQTAMPQYGCVWCEGERPRCVTREACGEAEAVATQCPAPLIHSVEPLTG PVDGGTRVTIRGSNLGQHVQDVLGMVTVAGVPCAVDAQEYEVSSSLVCITGASGEEVAGA TAVEVPGRGRGVSEHDFAYQDPKVHSIFPARGPRAGGTRLTLNGSKLLTGRLEDIRVVVG DQPCHLLPEQQSEQLRCETSPRPTPATLPVAVWFGATERRLQRGQFKYTLDPNITSAGPT KSFLSGGREICVRGQNLDVVQTPRIRVTVVSRMLQPSQGLGRRRRVVPETACSLGPSCSS QQFEEPCHVNSSQLITCRTPALPGLPEDPWVRVEFILDNLVFDFATLNPTPFSYEADPTL QPLNPEDPTMPFRHKPGSVFSVEGENLDLAMSKEEVVAMIGDGPCVVKTLTRHHLYCEPP VEQPLPRHHALREAPDSLPEFTVQMGNLRFSLGHVQYDGESPGAFPVAAQVGLGVGTSLL ALGVIIIVLMYRRKSKQALRDYKKVQIQLENLESSVRDRCKKEFTDLMTEMTDLTSDLLG SGIPFLDYKVYAERIFFPGHRESPLHRDLGVPESRRPTVEQGLGQLSNLLNSKLFLTKFI HTLESQRTFSARDRAYVASLLTVALHGKLEYFTDILRTLLSDLVAQYVAKNPKLMLRRTE TVVEKLLTNWMSICLYTFVRDSVGEPLYMLFRGIKHQVDKGPVDSVTGKAKYTLNDNRLL REDVEYRPLTLNALLAVGPGAGEAQGVPVKVLDCDTISQAKEKMLDQLYKGVPLTQRPDP RTLDVEWRSGVAGHLILSDEDVTSEVQGLWRRLNTLQHYKVPDGATVALVPCLTKHVLRE NQDYVPGERTPMLEDVDEGGIRPWHLVKPSDEPEPPRPRRGSLRGGERERAKAIPEIYLT RLLSMKGTLQKFVDDLFQVILSTSRPVPLAVKYFFDLLDEQAQQHGISDQDTIHIWKTNS LPLRFWINIIKNPQFVFDVQTSDNMDAVLLVIAQTFMDACTLADHKLGRDSPINKLLYAR DIPRYKRMVERYYADIRQTVPASDQEMNSVLAELSWNYSGDLGARVALHELYKYINKYYD QIITALEEDGTAQKMQLGYRLQQIAAAVENKVTDLL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
