Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859912
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5901nts
Open reading frame: 1 to 5901
ATGTGGATACCTACGGAGCACGAGAAATACGGCGTGGTTATTGCCAGTTTCCGAGGAACC
GTTCCATATGGCCTGTCATTGGAAATTGGAGATACAGTTCAGATCCTGGAGAAGTGTGAT
GGCTGGTACAGAGGATTTGCCTTAAAAAACCCAAATATCAAGGGTATATTTCCTTCCAGC
TACGTTCACTTGAAAAATGCCTGTGTAAAGAACAAAGGACAATTTGAAATGGTTATTCCC
ACTGAAGACTCTGTTATCACAGAAATGACATCAACATTAAGAGACTGGGGAACCATGTGG
AAACAACTCTATGTGCGTAATGAAGGCGATCTCTTCCACCGGCTGTGGCACATCATGAAT
GAAATCCTGGACCTGAGGCGGCAGGTGCTGGTGGGCCACCTCACCCACGACCGGATGAAG
GACGTGAAGCGCCACATTACTGCCCGGCTTGACTGGGGCAATGAACAACTGGGACTGGAC
CTGGTGCCTAGGAAAGAGTACGCAATGGTGGATCCGGAAGACATCAGCATTACTGAGCTC
TACCGATTGATGGAACATCGACATCGGAAGAAAGACACCCCGGTGCAGGCCAGCAGTCAC
CACCTCTTTGTCCAGATGAAGAGCCTCATGTGTTCCAACCTGGGAGAGGAGCTGGAGGTC
ATCTTCTCACTCTTTGACAGTAAAGAGAACCGGCCAATCAGTGAGAGATTTTTCTTGAGG
CTGAATAGAAACGGGCTTCCCAAAGCCCCTGATAAACCGGAACGACATTGCTCCCTCTTT
GTGGATTTGGGCAGCAGTGAGCTAAGAAAGGACATTTATATCACCGTGCACATTATCCGA
ATCGGTCGAATGGGAGCAGGAGAAAAAAAGAATGCCTGTAGTGTCCAGTACCGACGACCC
TTTGGCTGTGCAGTTCTTAGCATCGCTGACCTGCTAACAGGAGAGACAAAGGATGACCTC
ATTCTGAAAGTATACATGTGTAACACAGAGAGTGAGTGGTACCAAATCCATGAGAACATC
ATCAAAAAGCTGAATGCACGTTATAACTTGACTGGCTCCAATGCAGGTTTAGCAGTTTCC
TTACAGCTATTGCACGGAGACATTGAACAAATCAGAAGGGAATATTCATCAGTATTTTCT
CATGGAGTATCCATAACAAGGAAGCTGGGATTTTCAAATATTATTATGCCTGGTGAAATG
AGGAATGATTTATATATCACTATTGAAAGGGGAGAATTTGAGAAAGGAGGGAAGAGCGTG
GCCAGAAATGTGGAAGTTACGATGTTCATTGTAGACAGTAGTGGCCAAACCCTGAAGGAT
TTTATCTCCTTCGGCTCTGGGGAGCCACCAGCCAGTGAGTACCACTCCTTTGTGCTTTAC
CATAACAACAGTCCCAGGTGGTCTGAACTGCTGAAACTTCCCATTCCTGTGGATAAATTC
CGGGGTGCACACATCCGCTTCGAGTTTCGGCATTGTTCCACAAAGGAGAAAGGAGAGAAG
AAGTTGTTTGGGTTTTCTTTTGTCCCTCTGATGCAAGAAGATGGTAGGACTCTTCCAGAT
GGCACTCATGAGCTCATCGTGCATAAGTGTGAAGAAAACACAAATCTTCAGGATACTACC
CGCTACCTCAAACTTCCCTTTTCCAAGGGCATTTTCCTTGGGAATAATAATCAAGCCATG
AAGGCCACAAAGGAGTCCTTTTGTATTACATCTTTTCTCTGTTCCACAAAACTCACACAA
AATGGTGATATGCTTGATCTTTTGAAATGGAGAACCCACCCAGACAAGATCACTGGCTGT
CTCTCTAAATTAAAAGAAATTGATGGCTCAGAGATAGTAAAGTTTCTGCAGGATACACTG
GATACCTTATTTGGAATTTTAGATGAAAATTCCCAAAAATATGGGTCTAAAGTGTTTGAT
TCTTTGGTTCACATAATAAATTTGCTGCAAGATAGCAAATTTCATCATTTTAAACCTGTA
ATGGACACTTACATTGAGAGTCATTTTGCTGGGGCACTTGCATACAGAGATCTCATCAAA
GTGCTCAAATGGTACGTGGACCGGATCACAGAAGCAGAGCGGCAAGAGCATATCCAGGAG
GTGCTGAAGGCACAAGAATACATTTTTAAGTATATAGTTCAATCTCGAAGGCTGTTTTCC
CTTGCCACTGGTGGGCAAAACGAAGAGGAGTTCCGCTGCTGCATTCAGGAGCTTCTCATG
TCAGTCCGTTTCTTTCTTTCGCAAGAGAGCAAAGGGTCTGGAGCATTATCTCAGTCACAG
GCTGTGTTTCTGAGCTCTTTCCCTGCCGTGTACTCAGAACTGTTGAAGCTCTTTGATGTC
CGGGAAGTAGCCAACTTGGTCCAGGACACCCTGGGCAGTCTGCCGACCATCCTGCATGTG
GATGATTCCCTGCAGGCCATCAAACTGCAGTGCATTGGCAAAACCGTGGAAAGCCAGCTT
TATACCAACCCAGATTCCCGATACATTCTTCTGCCTGTCGTGTTACATCACCTCCACATT
CACTTGCAAGAACAGAAGGACCTGATCATGTGTGCACGTATCCTTAGCAACGTATTTTGT
CTTATCAAGAAAAATAGCTCAGAAAAATCTGTGCTGGAGGAAATAGATGTGATAGTGGCC
AGCTTGCTGGATATTCTGCTGAGGACCATATTGGAGATCACCAGCCGACCTCAGCCATCC
AGCTCAGCAATGCGGTTCCAGTTCCAGGATGTCACTGGGGAGTTTGTTGCTTGTCTCCTG
TCCCTATTACGACAAATGACAGATAGACATTATCAACAGCTTCTTGATAGTTTTAATACA
AAGGAAGAACTAAGGGATTTCCTGCTGCAGATATTTACTGTGTTCCGAATATTGATACGC
CCGGAGATGTTTCCAAAGGACTGGACTGTTATGCGCTTGGTTGCTAACAATGTTATTATT
ACAACAGTTCTATACCTCTCAGATGCACTTCGTAAGAACTTCTTAAATGAAAACTTTGAT
TATAAGATCTGGGATTCCTACTTTTACCTCGCAGTCATTTTTATAAACCAGTTGTGTCTG
CAGTTGGAGATGTTCACACCTTCCAAGAAGAAAAAGGTGTTAGAAAAGTATGGTGACATG
CGGGTAACAATGGGTTGTGAAATTTTCAGCATGTGGCAAAACCTAGGAGAGCACAAGCTT
CATTTTATCCCTGCCCTGATTGGCCCCTTCCTAGAAGTGACCTTGATACCCCAGCCAGAT
CTTCGGAATGTCATGATTCCAATTTTTCATGATATGATGGACTGGGAGCAGAGGCGGAGT
GGCAACTTTAAACAGGTGGAAGCCAAGCTAATTGACAAACTGGATAGCCTGATGTCAGAA
GGCAAAGGTGACGAAACATACCGCGAGCTCTTCAACAGTATTCTACTAAAGAAAATTGAG
CGGGAAACATGGCGGGAAAGTGGCGTTTCATTAATTGCTACTGTAACTCGTCTAATGGAG
AGGTTGTTAGATTACAGGGACTGCATGAAAATGGGAGAGGTAGATGGCAAAAAGATTGGC
TGCACAGTTAGCCTTCTGAACTTCTATAAGACTGAACTGAACAAGGAGGAGATGTATATA
CGCTACATTCACAAACTCTATGATCTGCATCTCAAAGCACAGAACTTTACAGAAGCTGCA
TATACCCTCCTCTTATATGACGAGCTACTGGAATGGTCTGATCGGCCCCTCAGGGAGTTC
CTGACCTACCCCATGCAAACAGAATGGCAGCGCAAAGAGCACCTGCACCTCACCATCATC
CAGAACTTTGACAGAGGCAAATGTTGGGAGAATGGCATTATCTTGTGCCGGAAGATTGCA
GAGCAGTATGAGAGTTATTATGACTACAGAAACCTGAGCAAGATGCGGATGATGGAAGCC
TCTTTGTATGACAAAATTATGGACCAGCAACGTCTTGAACCAGAGTTCTTCAGAGTTGGA
TTTTATGGAAAAAAATTTCCATTTTTCTTAAGAAATAAGGAGTTTGTGTGTCGAGGGCAT
GACTACGAGAGGCTGGAAGCCTTCCAACAGAGAATGCTGAACGAGTTCCCCCATGCCATC
GCCATGCAGCACGCCAACCAGCCCGATGAGACCATCTTCCAGGCAGAAGCTCAGTATTTG
CAGATATATGCTGTGACTCCCATTCCAGAGAGCCAGGAGGTCCTGCAGAGAGAGGGTGTT
CCGGACAACATCAAAAGCTTCTATAAAGTGAATCACATCTGGAAATTCCGCTATGACCGA
CCATTTCACAAAGGCACAAAAGATAAAGAGAATGAATTCAAGAGTCTCTGGGTGGAGAGA
ACGTCATTATACTTGGTGCAGAGTTTGCCTGGCATCTCTCGCTGGTTTGAAGTGGAAAAG
CGTGAAGTGGTAGAAATGAGTCCTCTGGAAAATGCAATTGAAGTGCTAGAAAATAAGAAT
CAGCAGCTGAAGACTCTGATTAGTCAGTGTCAGACAAGACAGATGCAGAATATTAATCCC
CTGACTATGTGCCTGAATGGAGTTATAGATGCTGCAGTTAATGGTGGCGTTTCCAGGTAT
CAAGAGGCATTCTTTGTCAAAGAATATATCTTAAGTCACCCTGAAGATGGGGAGAAAATT
GCACGATTAAGAGAGCTGATGCTTGAGCAGGCACAGATTCTGGAATTTGGTTTGGCCGTG
CATGAGAAGTTTGTACCTCAAGATATGAGACCCCTTCACAAAAAGCTGGTTGACCAATTC
TTTGTGATGAAGTCGAGCTTAGGGATACAGGAGTTCTCTGCTTGTATGCAAGCCAGTCCT
GTCCATTTTCCTAATGGAAGCCCTCGTGTGTGTAGAAACTCAGCACCTGCTTCTGTGAGC
CCAGATGGTACCAGGGTAATTCCTAGACGCAGCCCGTTAAGTTACCCAGCTGTCAACCGA
TATTCTTCCTCCTCACTGTCCTCACAAGCTTCTGCTGAAGTAAGCAATATTACAGGGCAA
TCAGAAAGCTCTGATGAAGTCTTTAACATGCAGCCAAGTCCATCTACCTCAAGCTTGAGT
TCTACTCACTCGGCTTCACCTAATGTGACAAGTTCTGCTCCATCGAGTGCCAGAGCTTCT
CCTTTGTTGTCTGACAAACACAAACATTCCCGAGAAAACTCTTGCCTGTCACCAAGAGAG
AGACCATGCAGTGCCATCTATCCAACACCTGTGGAGCCTTCGCAGAGGATGCTGTTTAAT
CATATTGGAGACGGGGCCTTGCCACGCAGTGACCCAAATCTCTCTGCACCTGAAAAAGCT
GTGAACCCCACCCCTAGCAGCTGGAGCCTGGACAGTGGGAAGGAAGCCAAGAACATGTCG
GATAGTGGGAAACTTATCTCTCCCCCTGTCCCTCCAAGACCCACACAGACTGCTTCACCA
GCAAGACACACGACATCAGTATCCCCCTCGCCTGCCGGGCGATCTCCATTGAAGGGCTCT
GTGCAGTCTTTCACCCCCTCTCCAGTGGAGTACCACTCGCCAGGACTCATCTCCAACTCC
CCTGTCTTGTCGGGCAGCTACAGCAGTGGGATTTCTTCTCTCAGCCGGTGCAGCACGTCG
GAAACCTCAGGCTTTGAAAATCAGGTGAATGAACAGTCGGCCCCCCTGCCGGTGCCAGTG
CCGGTGCCCGTGCCGAGCTACGGCGGGGAGGAGCCAGTGCGCAAGGAGAGCAAGACTCCG
CCCCCGTACAGCGTCTACGAGCGGACTCTGCGGCGCCCCGTCCCGCTACCTCACAGCCTC
TCCATCCCCGTCACGTCGGAGCCGCCCGCGCTGCCCCCCAAGCCTCTGGCAGCGCGATCC
AGCCACCTGGAGAATGGGGCCCGGAGGACTGACCCCGGCCCGCGGCCCAGGCCCCTGCCC
CGCAAGGTCTCTCAGTTATTG
Protein sequence predicted based on nucleotide sequence
MWIPTEHEKYGVVIASFRGTVPYGLSLEIGDTVQILEKCDGWYRGFALKNPNIKGIFPSS YVHLKNACVKNKGQFEMVIPTEDSVITEMTSTLRDWGTMWKQLYVRNEGDLFHRLWHIMN EILDLRRQVLVGHLTHDRMKDVKRHITARLDWGNEQLGLDLVPRKEYAMVDPEDISITEL YRLMEHRHRKKDTPVQASSHHLFVQMKSLMCSNLGEELEVIFSLFDSKENRPISERFFLR LNRNGLPKAPDKPERHCSLFVDLGSSELRKDIYITVHIIRIGRMGAGEKKNACSVQYRRP FGCAVLSIADLLTGETKDDLILKVYMCNTESEWYQIHENIIKKLNARYNLTGSNAGLAVS LQLLHGDIEQIRREYSSVFSHGVSITRKLGFSNIIMPGEMRNDLYITIERGEFEKGGKSV ARNVEVTMFIVDSSGQTLKDFISFGSGEPPASEYHSFVLYHNNSPRWSELLKLPIPVDKF RGAHIRFEFRHCSTKEKGEKKLFGFSFVPLMQEDGRTLPDGTHELIVHKCEENTNLQDTT RYLKLPFSKGIFLGNNNQAMKATKESFCITSFLCSTKLTQNGDMLDLLKWRTHPDKITGC LSKLKEIDGSEIVKFLQDTLDTLFGILDENSQKYGSKVFDSLVHIINLLQDSKFHHFKPV MDTYIESHFAGALAYRDLIKVLKWYVDRITEAERQEHIQEVLKAQEYIFKYIVQSRRLFS LATGGQNEEEFRCCIQELLMSVRFFLSQESKGSGALSQSQAVFLSSFPAVYSELLKLFDV REVANLVQDTLGSLPTILHVDDSLQAIKLQCIGKTVESQLYTNPDSRYILLPVVLHHLHI HLQEQKDLIMCARILSNVFCLIKKNSSEKSVLEEIDVIVASLLDILLRTILEITSRPQPS SSAMRFQFQDVTGEFVACLLSLLRQMTDRHYQQLLDSFNTKEELRDFLLQIFTVFRILIR PEMFPKDWTVMRLVANNVIITTVLYLSDALRKNFLNENFDYKIWDSYFYLAVIFINQLCL QLEMFTPSKKKKVLEKYGDMRVTMGCEIFSMWQNLGEHKLHFIPALIGPFLEVTLIPQPD LRNVMIPIFHDMMDWEQRRSGNFKQVEAKLIDKLDSLMSEGKGDETYRELFNSILLKKIE RETWRESGVSLIATVTRLMERLLDYRDCMKMGEVDGKKIGCTVSLLNFYKTELNKEEMYI RYIHKLYDLHLKAQNFTEAAYTLLLYDELLEWSDRPLREFLTYPMQTEWQRKEHLHLTII QNFDRGKCWENGIILCRKIAEQYESYYDYRNLSKMRMMEASLYDKIMDQQRLEPEFFRVG FYGKKFPFFLRNKEFVCRGHDYERLEAFQQRMLNEFPHAIAMQHANQPDETIFQAEAQYL QIYAVTPIPESQEVLQREGVPDNIKSFYKVNHIWKFRYDRPFHKGTKDKENEFKSLWVER TSLYLVQSLPGISRWFEVEKREVVEMSPLENAIEVLENKNQQLKTLISQCQTRQMQNINP LTMCLNGVIDAAVNGGVSRYQEAFFVKEYILSHPEDGEKIARLRELMLEQAQILEFGLAV HEKFVPQDMRPLHKKLVDQFFVMKSSLGIQEFSACMQASPVHFPNGSPRVCRNSAPASVS PDGTRVIPRRSPLSYPAVNRYSSSSLSSQASAEVSNITGQSESSDEVFNMQPSPSTSSLS STHSASPNVTSSAPSSARASPLLSDKHKHSRENSCLSPRERPCSAIYPTPVEPSQRMLFN HIGDGALPRSDPNLSAPEKAVNPTPSSWSLDSGKEAKNMSDSGKLISPPVPPRPTQTASP ARHTTSVSPSPAGRSPLKGSVQSFTPSPVEYHSPGLISNSPVLSGSYSSGISSLSRCSTS ETSGFENQVNEQSAPLPVPVPVPVPSYGGEEPVRKESKTPPPYSVYERTLRRPVPLPHSL SIPVTSEPPALPPKPLAARSSHLENGARRTDPGPRPRPLPRKVSQLL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
