Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859914
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 7713nts
Open reading frame: 1 to 7713
ATGGCGGGGCCCCGGGGCCTCCTCCCACTCTGCCTCCTGGCCTTCTGCCTGGCAGGCTTC
AGCTTCGTCAGGGGGCAGGTGCTGTTCAAAGGCTGTGATGTGAAAACCACGTTTGTCACT
CATGTACCCTGCACCTCGTGCGCGGCCATCAAGAAGCAGACGTGTCCCTCAGGCTGGCTG
CGGGAGCTCCCGGATCAGATAACCCAGGACTGCCGCTACGAAGTACAGCTGGGGGGCTCT
ATGGTGTCCATGAGCGGCTGCAGACGGAAGTGCCGGAAGCAAGTGGTGCAGAAGGCCTGC
TGCCCTGGCTACTGGGGTTCCCGGTGCCATGAATGCCCTGGGGGCGCTGAGACCCCATGC
AATGGCCACGGGACCTGCTTGGATGGCATGGACAGGAATGGGACCTGTGTGTGCCAGGAA
AACTTCCGCGGCTCAGCCTGCCAGGAGTGCCAAGACCCCAACCGGTTCGGGCCTGACTGC
CAATCGGTGTGCAGCTGTGTGCACGGAGTGTGCAACCATGGGCCACGTGGGGATGGAAGC
TGCCTGTGCTTTGCTGGATACACTGGCCCCCACTGTGATCAAGAGCTGCCCGTCTGCCAG
GAGCTGCGCTGTCCCCAGAACACCCAGTGCTCCGCAGAGGCTCCCAGCTGCAGGTGCCTG
CCCGGCTACACACAGCAGGGCAGTGAATGCCGAGCCCCCAACCCCTGCTGGCCATCACCC
TGCTCACTGCTGGCCCAGTGCTCGGTGAGCCCCAAGGGGCAGGCTCAGTGTCACTGCCCT
GAGAACTACCATGGCGATGGGATGGTGTGTCTGCCCAAGGACCCATGCACTGACAACCTT
GGTGGCTGCCCCAGCAACTCTACTTTGTGTGTGTACCAGAAGCCGGGCCAGGCCTTCTGC
ACCTGCCGGCCAGGCCTGGTCAGCATCAACAGCAACGCTTCTGCGGGCTGCTTCGCCTTC
TGCTCCCCCTTCTCCTGCGACCGGTCTGCCACTTGCCAGGTGACCGCTGATGGGAAGACC
AGCTGTGTGTGCAGGGAAAGCGAGGTGGGGGATGGGCGTGCCTGCTACGGACACCTGCTC
CACGAGGTGCAGAAGGCCACGCAGACAGGCCGGGTGTTCCTGCAGCTGAGGGTCGCCGTG
GCCATGATGGACCAGGGCTGCCGGGAAATCCTTACCACAGCGGGCCCTTTCACCGTGCTG
GTGCCATCCGTCTCCTCCTTCTCCTCCAGGACCATGAATGCATCCCTTGCCCAGCAGCTC
TGTAGACAGCACATCATCGCAGGGCAGCACATCCTGGAGGACACAAGGACCCAACAAACA
CGAAGGTGGTGGACGCTGGCCGGGCAGGAGATCACCGTCACCTTTAACCAATTCACGAAA
TACTCCTACAAGTACAAAGACCAGCCCCAGCAGACGTTCAACATCTACAAGGCCAACAAC
ATAGCAGCTAATGGCGTCTTCCACGTGGTCACTGGCCTGCGGTGGCAGGCCCCCTCTGGG
ACCCCTGGGGATCCCAAGAGAACTATCGGACAGATCCTCGCCTCTACCGAGGCCTTCAGC
CGCTTTGAAACCATCCTGGAGAACTGTGGGCTGCCCTCCATCCTGGACGGACCTGGGCCC
TTCACAGTCTTTGCCCCAAGCAATGAGGCTGTGGACAGCTTGCGTGACGGCCGCCTGATC
TACCTCTTCACAGCGGGTCTCTCTAAACTGCAGGAGTTGGTGCGGTACCACATCTACAAC
CACGGCCAGCTGACCGTTGAGAAGCTCATCTCCAAGGGTCGGATCCTCACCATGGCGAAC
CAGGTCCTGGCTGTGAACATTTCTGAGGAGGGGCGCATCCTGCTGGGACCCGAGGGGGTC
CCGCTGCAGAGGGTAGACGTGATGGCCGCCAATGGTGTGATCCACATGCTGGACGGCATC
CTGCTGCCCCCGACCATCCTGCCCATCCTGCCCAAGCACTGCAGCGAGGAGCAGCACAAG
ATTGTGGCGGGCTCCTGTGTGGACTGCCAAGCCCTGAACACCAGCACGTGTCCCCCCAAC
AGTGTGAAGCTGGACATCTTCCCCAAGGAGTGTGTCTACATCCATGACCCAACGGGGCTC
AATGTGCTAAAGAAGGGCTGTGCCAGCTACTGCAACCAAACCATCATGGAACAAGGCTGC
TGCAAAGGTTTTTTCGGGCCTGACTGCACGCAGTGTCCTGGGGGCTTCTCCAACCCCTGC
TATGGCAAAGGCAATTGCAGTGATGGGATCCAGGGCAATGGGGCCTGCCTCTGCTTCCCA
GACTACAAGGGCATCGCCTGCCACATCTGCTCGAACCCAAACAAGCATGGAGAGCAATGC
CAGGAAGACTGCGGCTGTGTCCATGGTCTCTGCGACAACCGCCCAGGCAGTGGGGGGGTG
TGCCAGCAGGGCACGTGTGCCCCTGGCTTCAGTGGCCGGTTCTGCAACGAGTCCATGGGG
GACTGTGGGCCCACAGGGCTGGCCCAGCACTGCCACCTGCATGCCCGCTGTGTTAGCCAG
GAGGGTGTTGCCAGATGTCGCTGTCTTGATGGCTTTGAGGGTGATGGCTTCTCCTGCACA
CCTAGCAACCCCTGCTCCCACCCGGACCGTGGAGGCTGCTCAGAGAATGCTGAGTGTGTC
CCTGGGTCCCTGGGCACCCACCACTGCACATGCCACAAAGGCTGGAGTGGGGATGGCCGC
GTCTGTGTGGCTATTGACGAGTGTGAGCTGGACATGAGAGGTGGCTGCCACACCGATGCC
CTCTGCAGCTATGTGGGCCCCGGGCAGAGCCGATGCACCTGCAAGCTGGGCTTTGCCGGG
GATGGCTACCAGTGCAGCCCCATCGACCCCTGCCGGGCAGGCAATGGCGGCTGCCACGGC
CTGGCCACCTGCCGGGCAGTGGGGGGAGGTCAGCGGGTCTGCACGTGCCCCCCTGGCTTT
GGGGGTGATGGCTTCAGCTGTTATGGAGACATCTTCCGGGAGCTGGAGGCAAATGCCCAC
TTCTCCATCTTCTACCAATGGCTTAAGAGTGCCGGCATCACGCTTCCTGCCGACCGCCGA
GTCACAGCCCTGGTGCCCTCCGAGGCTGCAGTCCGTCAGCTGAGCCCCGAGGACCGAGCT
TTCTGGCTGCAGCCAAGGACGCTGCCGAACCTGGTCAGGGCCCATTTTCTCCAGGGTGCC
CTCTTCGAGGAGGAGCTGGCCCGGCTGGGTGGGCAGGAAGTGGCCACCCTGAACCCCACC
ACACGCTGGGAGATTCGCAACATTAGTGGGAGGGTCTGGGTGCAGAATGCCAGCGTGGAT
GTGGCTGACCTCCTTGCCACCAACGGTGTCCTACACATCCTCAGCCAGGTCTTACTGCCC
CCCCGAGGGGATGTGCCCGGTGGGCAGGGGTTGCTGCAGCAGCTGGACTTGGTGCCTGCC
TTCAGCCTCTTCCGGGAATTGCTGCAGCACCATGGGTTGGTGCCCCAGATTGAGGCTGCC
ACTGCCTACACCATCTTTGTGCCCACCAACCGCTCCCTGGAGGCCCAGGGCAACAGCAGT
CACCTGGACGCAGACACAGTGCGGCACCATGTGGTCCTGGGGGAGGCCCTCTCCATGGAA
ACCCTGCGGAAGGGTGGACACCGCAACTCCCTCCTGGGCCCTGCCCACTGGATCGTCTTC
TACAACCACAGTGGCCAGCCTGAGGTGAACCATGTGCCACTGGAAGGCCCCATGCTGGAG
GCCCCTGGCCGCTCGCTGATTGGTCTGTCGGGGGTCCTGACGGTGGGCTCAAGTCGCTGC
CTGCATAGCCACGCTGAGGCCCTGCGGGAGAAATGTGTAAACTGCACCAGGAGATTCCGC
TGCACTCAGGGCTTCCAGCTGCAGGACACACCCAGGAAGAGCTGTGTCTACCGATCTGGC
TTCTCCTTCTCCCGGGGCTGCTCTTACACATGTGCCAAGAAGATCCAGGTGCCGGACTGC
TGCCCTGGTTTCTTTGGCACGCTGTGTGAGCCATGCCCAGGGGGTCTAGGGGGGGTGTGC
TCAGGCCATGGGCAGTGCCAGGACAGGTTCCTGGGCAGCGGGGAGTGCCACTGCCACGAG
GGCTTCCATGGAACGGCCTGTGAGGTGTGTGAGCTGGGCCGCTACGGGCCCAACTGCACC
GGAGTGTGTGACTGTGCCCATGGGCTGTGCCAGGAGGGGCTGCAAGGGGACGGAAGCTGT
GTCTGTAACGTGGGCTGGCAGGGCCTCCGCTGTGACCAGAAAATCACCAGCCCTCAGTGC
CCTAGGAAGTGCGACCCCAATGCCAACTGCGTGCAGGACTCGGCCGGAGCCTCCACCTGC
GCCTGTGCTGCGGGATACTCCGGCAATGGCATCTTCTGTTCAGAGGTGGACCCCTGCGCC
CACGGCCATGGGGGCTGCTCCCCTCATGCCAACTGTACCAAGGTGGCACCTGGGCAGCGG
ACATGCACCTGCCAGGATGGCTACATGGGCGACGGGGAGCTGTGCCAGGAAATTAACAGC
TGTCTCATCCACCACGGGGGCTGCCACATTCACGCCGAGTGCATCCCCACTGGCCCCCAG
CAGGTCTCCTGCAGCTGCCGTGAGGGTTACAGCGGGGATGGCATCCGGACCTGCGAGCTC
CTGGACCCCTGCTCTAAGAACAATGGAGGATGCAGCCCATATGCCACCTGCAAAAGCACA
GGGGATGGCCAGAGGACATGTACCTGCGACACAGCCCACACCGTGGGGGACGGCCTCACC
TGCCGTGCCCGAGTCGGCCTGGAGCTCCTGAGGGATAAGCATGCCTCATTCTTCAGCCTC
CGCCTCCTGGAATATAAGGAGCTCAAGGGCGATGGGCCTTTCACCATCTTCGTGCCGCAC
GCAGATCTAATGAGCAACCTGTCGCAGGATGAGCTGGCCCGGATTCGTGCGCATCGCCAG
CTGGTGTTTCGCTACCACGTGGTTGGCTGTCGGCGGCTGCGGAGCGAGGACCTGCTGGAG
CAGGGGTACGCCACGGCCCTCTCAGGGCACCCACTGCGCTTCAGCGAGAGGGAGGGCAGC
ATATACCTCAATGACTTCGCGCGCGTGGTGAGCAGCGACCATGAGGCCGTGAACGGCATC
CTGCACTTCATTGACCGTGTCCTGCTGCCCCCCGAGGCGCTGCACTGGGAGCCTGATGAT
GCTCCCATCCCGAGGAGAAATGTCACCGCCGCCGCCCAGGGCTTCGGTTACAAGATCTTC
AGCGGCCTCCTGAAGGTGGCCGGCCTCCTGCCCCTGCTTCGAGAGGCATCCCATAGGCCC
TTCACAATGCTGTGGCCCACAGACGCCGCCTTTCGAGCTCTGCCTCCGGATCGCCAGGCC
TGGCTGTACCATGAGGACCACCGTGACAAGCTAGCAGCCATTCTGCGGGGCCACATGATT
CGCAATGTCGAGGCCTTGGCATCTGACCTGCCCAACCTGGGCCCACTTCGAACCATGCAT
GGGACCCCCATCTCTTTCTCCTGCAGCCGAACGCGGGCCGGTGAGCTCATGGTGGGTGAG
GATGATGCTCGCATTGTGCAGCGGCACTTGCCCTTTGAGGGTGGCCTGGCCTATGGCATC
GACCAGCTGCTGGAGCCACCTGGCCTTGGTGCTCGCTGTGACCACTTTGAGACCCGGCCC
CTGCGACTGAACACCTGCAGCATCTGTGGGCTGGAGCCACCCTGTCCTGAGGGGTCACAG
GAGCAGGGCAGCCCTGAGGCCTGCTGGCGCTTCTACCCGAAGTTCTGGACGTCCCCTCCG
CTGCACTCTTTGGGATTACGCAGCGTCTGGGTCCACCCCAGCCTTTGGGGTAGGCCCCAA
GGCCTGGGCAGGGGCTGCCACCGCAATTGTGTCACCACCACCTGGAAGCCCAGCTGCTGC
CCTGGTCACTATGGCAGTGAGTGCCAAGCTTGCCCTGGCGGCCCCAGCAGCCCTTGTAGT
GACCGTGGCGTGTGCATGGACGGCATGAGTGGCAGTGGGCAGTGTCTGTGCCGTTCAGGT
TTTGCTGGGACAGCCTGTGAACTCTGTGCTCCTGGTGCCTTTGGGCCCCATTGTCAAGCC
TGCCGCTGCACTGTGCATGGCCGCTGTGATGAGGGCCTTGGGGGCTCTGGCTCCTGCTTC
TGTGATGAAGGCTGGACTGGGCCACGCTGTGAGGTGCAACTGGAGCTGCAGCCTGTGTGT
ACCCCACCCTGTGCACCCGAGGCTGTGTGCCGTGCAGGCAACAGCTGTGAGTGCAGCCTG
GGCTATGAAGGGGATGGCCGTGTGTGTACAGTGGCAGACCTGTGCCAGGACGGGCATGGT
GGCTGCAGTGAGCACGCCAACTGTAGCCAGGTAGGAACAATGGTCACTTGTACCTGCCTG
CCCGACTACGAGGGTGATGGCTGGAGCTGCCGGGCCCGCAACCCCTGCACAGATGGCCAC
CGCGGGGGCTGCAGCGAGCACGCCAACTGCTTGAGCACCGGCCTGAACACACGGCGCTGT
GAGTGCCACGCAGGCTACGTAGGCGATGGACTGCAGTGTCTGGAGGAGTCGGAACCACCT
GTGGACCGCTGCTTGGGCCAGCCACCGCCCTGCCACTCAGATGCCATGTGCACTGACCTG
CACTTCCAGGAGAAACGGGCTGGCGTTTTCCACCTCCAGGCCACCAGCGGCCCTTATGGT
CTGAACTTTTCGGAGGCTGAGGCGGCATGCGAAGCACAGGGAGCCGTCCTTGCTTCATTC
CCTCAGCTCTCTGCTGCCCAGCAGCTGGGCTTCCACCTGTGCCTCATGGGCTGGCTGGCC
AATGGCTCCACTGCCCACCCTGTGGTTTTCCCTGTGGCGGACTGTGGCAATGGTCGGGTG
GGCATAGTCAGCCTGGGTGCCCGCAAGAACCTCTCAGAACGCTGGGATGCCTACTGCTTC
CGTGTGCAAGATGTGGCCTGCCGATGCCGAAATGGCTTCGTGGGTGACGGGATCAGCACG
TGCAATGGGAAGCTGCTGGATGTGCTGGCTGCCACTGCCAACTTCTCCACCTTCTATGGG
ATGCTATTGGGCTATGCCAATGCCACCCAGCGGGGTCTCGACTTCCTGGACTTCCTGGAT
GATGAGCTCACGTATAAGACACTCTTCGTCCCTGTCAATGAAGGCTTTGTGGACAACATG
ACGCTGAGTGGCCCAGACTTGGAGCTGCATGCCTCCAACGCCACCCTCCTAAGTGCCAAC
GCCAGCCAGGGGAAGTTGCTTCCGGCCCACTCAGGCCTCAGCCTCATCATCAGTGACGCA
GGCCCTGACAACAGTTCCTGGGCCCCTGTGGCCCCAGGGACAGTTGTGGTTAGCCGTATC
ATTGTGTGGGACATCATGGCCTTCAATGGCATCATCCATGCTCTGGCCAGCCCCCTCCTG
GCACCCCCACAGCCCCAGGCAGTGCTGGCGCCTGAAGCCCCACCTGTGGCGGCAGGCGTG
GGGGCTGTGCTTGCCGCTGGAGCACTGCTTGGCTTGGTGGCCGGAGCTCTCTACCTCCGT
GCCCGAGGCAAGCCCATGGGCTTTGGCTTCTCTGCCTTCCAGGCGGAAGATGATGCTGAT
GACGACTTCTCACCGTGGCAAGAAGGGACCAACCCCACCCTGGTCTCTGTCCCCAACCCT
GTCTTTGGCAGCGACACCTTTTGTGAACCCTTCGATGACTCACTGCTGGAGGAGGACTTC
CCTGACACCCAGAGGATCCTCACAGTCAAGTTG
Protein sequence predicted based on nucleotide sequence
MAGPRGLLPLCLLAFCLAGFSFVRGQVLFKGCDVKTTFVTHVPCTSCAAIKKQTCPSGWL RELPDQITQDCRYEVQLGGSMVSMSGCRRKCRKQVVQKACCPGYWGSRCHECPGGAETPC NGHGTCLDGMDRNGTCVCQENFRGSACQECQDPNRFGPDCQSVCSCVHGVCNHGPRGDGS CLCFAGYTGPHCDQELPVCQELRCPQNTQCSAEAPSCRCLPGYTQQGSECRAPNPCWPSP CSLLAQCSVSPKGQAQCHCPENYHGDGMVCLPKDPCTDNLGGCPSNSTLCVYQKPGQAFC TCRPGLVSINSNASAGCFAFCSPFSCDRSATCQVTADGKTSCVCRESEVGDGRACYGHLL HEVQKATQTGRVFLQLRVAVAMMDQGCREILTTAGPFTVLVPSVSSFSSRTMNASLAQQL CRQHIIAGQHILEDTRTQQTRRWWTLAGQEITVTFNQFTKYSYKYKDQPQQTFNIYKANN IAANGVFHVVTGLRWQAPSGTPGDPKRTIGQILASTEAFSRFETILENCGLPSILDGPGP FTVFAPSNEAVDSLRDGRLIYLFTAGLSKLQELVRYHIYNHGQLTVEKLISKGRILTMAN QVLAVNISEEGRILLGPEGVPLQRVDVMAANGVIHMLDGILLPPTILPILPKHCSEEQHK IVAGSCVDCQALNTSTCPPNSVKLDIFPKECVYIHDPTGLNVLKKGCASYCNQTIMEQGC CKGFFGPDCTQCPGGFSNPCYGKGNCSDGIQGNGACLCFPDYKGIACHICSNPNKHGEQC QEDCGCVHGLCDNRPGSGGVCQQGTCAPGFSGRFCNESMGDCGPTGLAQHCHLHARCVSQ EGVARCRCLDGFEGDGFSCTPSNPCSHPDRGGCSENAECVPGSLGTHHCTCHKGWSGDGR VCVAIDECELDMRGGCHTDALCSYVGPGQSRCTCKLGFAGDGYQCSPIDPCRAGNGGCHG LATCRAVGGGQRVCTCPPGFGGDGFSCYGDIFRELEANAHFSIFYQWLKSAGITLPADRR VTALVPSEAAVRQLSPEDRAFWLQPRTLPNLVRAHFLQGALFEEELARLGGQEVATLNPT TRWEIRNISGRVWVQNASVDVADLLATNGVLHILSQVLLPPRGDVPGGQGLLQQLDLVPA FSLFRELLQHHGLVPQIEAATAYTIFVPTNRSLEAQGNSSHLDADTVRHHVVLGEALSME TLRKGGHRNSLLGPAHWIVFYNHSGQPEVNHVPLEGPMLEAPGRSLIGLSGVLTVGSSRC LHSHAEALREKCVNCTRRFRCTQGFQLQDTPRKSCVYRSGFSFSRGCSYTCAKKIQVPDC CPGFFGTLCEPCPGGLGGVCSGHGQCQDRFLGSGECHCHEGFHGTACEVCELGRYGPNCT GVCDCAHGLCQEGLQGDGSCVCNVGWQGLRCDQKITSPQCPRKCDPNANCVQDSAGASTC ACAAGYSGNGIFCSEVDPCAHGHGGCSPHANCTKVAPGQRTCTCQDGYMGDGELCQEINS CLIHHGGCHIHAECIPTGPQQVSCSCREGYSGDGIRTCELLDPCSKNNGGCSPYATCKST GDGQRTCTCDTAHTVGDGLTCRARVGLELLRDKHASFFSLRLLEYKELKGDGPFTIFVPH ADLMSNLSQDELARIRAHRQLVFRYHVVGCRRLRSEDLLEQGYATALSGHPLRFSEREGS IYLNDFARVVSSDHEAVNGILHFIDRVLLPPEALHWEPDDAPIPRRNVTAAAQGFGYKIF SGLLKVAGLLPLLREASHRPFTMLWPTDAAFRALPPDRQAWLYHEDHRDKLAAILRGHMI RNVEALASDLPNLGPLRTMHGTPISFSCSRTRAGELMVGEDDARIVQRHLPFEGGLAYGI DQLLEPPGLGARCDHFETRPLRLNTCSICGLEPPCPEGSQEQGSPEACWRFYPKFWTSPP LHSLGLRSVWVHPSLWGRPQGLGRGCHRNCVTTTWKPSCCPGHYGSECQACPGGPSSPCS DRGVCMDGMSGSGQCLCRSGFAGTACELCAPGAFGPHCQACRCTVHGRCDEGLGGSGSCF CDEGWTGPRCEVQLELQPVCTPPCAPEAVCRAGNSCECSLGYEGDGRVCTVADLCQDGHG GCSEHANCSQVGTMVTCTCLPDYEGDGWSCRARNPCTDGHRGGCSEHANCLSTGLNTRRC ECHAGYVGDGLQCLEESEPPVDRCLGQPPPCHSDAMCTDLHFQEKRAGVFHLQATSGPYG LNFSEAEAACEAQGAVLASFPQLSAAQQLGFHLCLMGWLANGSTAHPVVFPVADCGNGRV GIVSLGARKNLSERWDAYCFRVQDVACRCRNGFVGDGISTCNGKLLDVLAATANFSTFYG MLLGYANATQRGLDFLDFLDDELTYKTLFVPVNEGFVDNMTLSGPDLELHASNATLLSAN ASQGKLLPAHSGLSLIISDAGPDNSSWAPVAPGTVVVSRIIVWDIMAFNGIIHALASPLL APPQPQAVLAPEAPPVAAGVGAVLAAGALLGLVAGALYLRARGKPMGFGFSAFQAEDDAD DDFSPWQEGTNPTLVSVPNPVFGSDTFCEPFDDSLLEEDFPDTQRILTVKL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
