Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860000
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 7260nts
Open reading frame: 1 to 7260
ATGGAGCAATTTCCAAAGGAAACCGTTGTGGAGAGCAGTGGGCCAAAGGTTTTGGAAACA
GCAGAAGAGATCCAGGAGAGGCGTCAGGAAGTGTTGACTCGGTATCAAAGTTTCAAGGAG
CGGGTCGCTGAGAGGGGTCAGAAGCTTGAGGATTCCTATCACTTACAAGTTTTCAAGCGA
GATGCAGATGATCTGGGGAAGTGGATCATGGAGAAAGTCAATATCTTAACCGATAAGAGC
TATGAAGACCCAACTAATATACAGGGGAAATATCAGAAGCATCAATCCCTTGAAGCAGAG
GTGCAAACAAAATCAAGACTCATGTCTGAACTGGAAAAAACAAGGGAAGAACGATTTACC
ATGGGTCATTCTGCCCACGAAGAAACGAAGGCCCATATAGAGGAGCTACGCCACCTGTGG
GACCTGCTGTTAGAGCTGACCCTGGAGAAGGGTGACCAGTTGCTGCGGGCCCTGAAGTTC
CAGCAGTATGTACAGGAGTGTGCTGACATCTTAGAGTGGATTGGAGACAAGGAGGCTATA
GCGACATCAGTGGAGCTAGGTGAAGACTGGGAGCGCACCGAAGTTCTGCATAAGAAATTT
GAAGACTTCCAAGTGGAGCTGGTAGCTAAAGAAGGGAGAGTTGTTGAAGTGAACCAATAT
GCCAATGAGTGTGCCGAGGAAAACCATCCTGACCTACCCTTAATTCAGTCTAAGCAAAAT
GAGGTGAATGCTGCCTGGGAGCGCCTTCGTGGTTTGGCTCTCCAGAGACAGAAAGCTCTG
TCCAATGCTGCAAACTTACAACGATTCAAAAGGGATGTGACTGAAGCCATCCAGTGGATC
AAGGAGAAGGAACCTGTACTCACCTCTGAGGACTATGGCAAAGACCTTGTTGCCTCTGAA
GGACTGTTTCACAGTCACAAGGGACTTGAGAGAAATCTTGCTGTCATGAGTGACAAGGTG
AAGGAGTTATGTGCTAAAGCAGAGAAGCTGACACTTTCCCATCCTTCAGATGCACCTCAG
ATCCAGGAGATGAAAGAAGATCTGGTCTCCAGCTGGGAGCATATTCGTGCCCTGGCCACC
AGCAGATATGAAAAACTGCAGGCTACTTATTGGTACCATCGATTTTCATCTGACTTTGAT
GAACTCTCAGGCTGGATGAACGAGAAGACTGCTGCGATCAATGCTGATGAGCTGCCAACA
GATGTGGCTGGTGGAGAAGTTCTGCTGGACAGGCATCAGCAGCATAAGCATGAGATTGAC
TCTTACGATGACCGATTTCAATCTGCTGATGAGACTGGTCAAGACCTCGTGAATGCCAAT
CATGAAGCCTCTGATGAAGTTCGGGAAAAGATGGAAATACTTGACAACAACTGGACTGCC
CTGCTGGAACTGTGGGACGAGCGTCATCGTCAGTATGAGCAGTGCTTGGACTTTCATCTC
TTCTACAGAGACAGTGAGCAAGTGGACAGTTGGATGAGTAGACAAGAGGCCTTCCTGGAA
AACGAGGATCTGGGAAACTCACTGGGCAGTGCAGAAGCCCTTCTTCAGAAGCATGAAGAC
TTTGAGGAAGCCTTTACTGCCCAGGAAGAGAAGATCATAACTGTAGACAAGACTGCAACC
AAATTGATTGGTGATGACCATTATGATTCAGAGAACATCAAGGCTATCCGTGACGGGCTG
TTAGCCCGGCGGGATGCCCTACGTGAAAAGGCTGCCACTAGACGTAGATTGCTGAAGGAG
TCATTGCTTCTGCAAAAACTGTATGAGGACTCAGATGACCTAAAGAACTGGATCAACAAG
AAGAAAAAGTTGGCAGATGATGAAGATTACAAGGACATACAGAACTTGAAGAGCAGGGTT
CAAAAGCAGCAAGTCTTTGAAAAGGAGTTGGCAGTTAATAAGACCCAGCTGGAAAACATA
CAGAAAACTGGCCAAGAGATGATTGAGGGTGGTCACTATGCCTCTGACAATGTGACCACT
CGTCTGAGTGAAGTTGCCAGCCTCTGGGAGGAGTTGCTGGAGGCTACAAAACAGAAAGGG
ACCCAGTTGCATGAGGCCAACCAGCAGCTGCAATTTGAAAATAATGCAGAAGATTTGCAG
CGCTGGCTGGAGGATGTTGAGTGGCAAGTCACCTCTGAGGATTATGGGAAAGGCCTGGCC
GAGGTACAGAATCGACTCAGGAAACACGGCCTCCTGGAGTCGGCTGTGGCTGCTCGTCAG
GATCAGGTGGATATCCTTACAGACCTGGCTGCATATTTTGAAGAAATAGGCCATCCTGAT
TCTAAGGATATAAGGGCAAGGCAAGAGTCCTTGGTATGCCGATTTGAAGCTCTGAAAGAG
CCACTGGCCACCCGAAAGAAGAAGCTCTTAGACCTTCTCCATCTGCAGCTGATTTGTAGA
GACACAGAGGATGAGGAGGCCTGGATCCAAGAGACTGAACCCTCAGCTACTTCCACCTAC
CTTGGAAAGGACCTGATTGCTTCCAAAAAGCTTCTGAATAGGCATAGAGTCATCCTGGAG
AACATTGCCAGCCATGAACCACGCATTCAAGAGATAACAGAAAGGGGAAACAAAATGGTA
GAGGAAGGACACTTTGCTGCAGAAGATGTGGCCTCTAGGGTCAAGAGTTTGAACCAGAAT
ATGGAGTCTCTCCGTGCTCGAGCTGCTAGGCGACAAAATGATCTTGAAGCCAATGTCCAG
TTCCAGCAGTACCTGGCTGACCTGCATGAAGCAGAAACATGGATCAGAGAGAAGGAACCT
ATTGTAGATAATACTAACTATGGTGCTGATGAAGAAGCAGCTGGGGCTCTTCTAAAGAAG
CATGAGGCCTTTCTATTAGATCTCAATTCATTTGGAGACAGTATGAAAGCTCTGCGGAAT
CAGGCAAACGCCTGCCAGCAACAACAGGCTGCACCAGTGGAGGGAGTTGCTGGAGAACAA
AGGGTCATGGCTTTATATGACTTCCAGGCCCGCAGCCCCCGAGAAGTCACCATGAAGAAA
GGTGATGTCTTAACGCTGCTCAGTTCCATCAATAAGGACTGGTGGAAGGTGGAAGCTGCT
GATCATCAGGGCATTGTCCCAGCTGTCTATGTCAGAAGACTGGCCCACGATGAGTTCCCG
ATGCTCCCACAGCGGCGACGAGAAGAGCCAGGAAACATCACCCAGCGCCAGGAGCAGATT
GAGAACCAATACCGCTCCCTCTTGGATCGGGCAGAAGAACGCAGACGTCGTCTATTGCAA
CGTTATAATGAATTTTTATTGGCCTATGAGGCAGGAGACATGCTGGAATGGATTCAAGAG
AAAAAGGCAGAAAACACTGGAGTGGAACTAGATGATGTTTGGGAGCTGCAGAAAAAGTTT
GATGAGTTCCAAAAGGATTTGAATACCAATGAGCCTCGGCTAAGGGATATCAACAAGGTA
GCTGATGATCTACTATTTGAAGGACTTCTAACACCAGAAGGAGCTCAAATCCGGCAGGAA
TTGAATTCCCGCTGGGGTTCTTTGCAGAGGCTTGCAGATGAACAGCGGCAGCTGCTGGGC
AGTGCCCATGCTGTTGAAGTGTTTCACAGAGAAGCAGATGACACGAAGGAGCAGATTGAG
AAGAAATGCCAGGCCCTCAGTGCTGCAGACCCTGGCTCAGATCTGTTCAGTGTTCAGGCT
CTTCAGCGACGGCATGAGGGCTTTGAAAGGGACCTCGTACCCCTGGGAGATAAGGTGACC
ATACTGGGGGAGACAGCAGAGCGGCTCAGTGAGTCCCATCCAGATGCCACTGAGGACCTG
CAGAGACAGAAAATGGAGCTGAATGAGGCCTGGGAAGACCTGCAGGGGCGTACAAAGGAT
CGTAAGGAGAGCCTAAATGAGGCCCAGAAATTCTACCTGTTCCTCAGCAAGGCCAGGGAT
CTGCAGAACTGGATCAGTAGCATTGGTGGCATGGTATCATCACAGGAGCTGGCCGAAGAC
TTAACTGGCATAGAGATCTTGCTGGAGAGACATCAGGAGCACCGTGCTGACATGGAGGCA
GAGGCTCCCACCTTCCAGGCCTTAGAGGACTTCAGTGCAGAACTTATCGACAGTGGGCAC
CATGCTAGCCCTGAAATTGAAAAAAAGCTTCAAGCTGTCAAGCTAGAGAGAGATGATTTG
GAGAAGGCTTGGGAAAAACGCAAGAAGATCCTAGACCAGTGCCTGGAGTTGCAGATGTTC
CAGGGGAACTGTGATCAAGTTGAGAGCTGGATGGTGGCACGTGAGAATTCCCTGAGGTCA
GATGACAAAAGTTCCTTAGACAGTCTGGAGGCTTTGATGAAGAAACGGGACGATTTGGAC
AAAGCAATCACTGCCCAGGAAGGGAAGATCACTGACCTAGAACATTTTGCTGAGAGCCTC
ATTGCTGATGAACACTATGCCAAAGAAGAGATTGCTACGCGGCTCCAACGTGTACTAGAC
AGGTGGAAGGCTCTCAAAGCACAACTGATTGATGAGCGGACAAAGCTTGGAGACTATGCC
AACCTAAAACAATTCTACCGAGACCTTGAGGAGCTGGAAGAATGGATCAGTGAGATGCTG
CCCACAGCCTGTGATGAATCCTACAAAGACGCCACTAACATTCAGAGGAAATACCTGAAA
CACCAGACCTTTGCACATGAAGTCGATGGCCGATCTGAGCAGGTGCATGGCGTCATCAAC
CTGGGGAACTCCCTGATTGAGTGTAGCGCTTGTGATGGCAATGAAGAGGCCATGAAGGAG
CAACTGGAACAGCTGAAGGAACATTGGGATCATCTGCTTGAGAGAACAAATGACAAAGGG
AAGAAGCTCAATGAGGCCAGTCGTCAACAGAGGTTCAACACAAGCATCCGGGACTTTGAG
TTCTGGCTCTCAGAGGCAGAGACATTGCTGGCCATGAAAGATCAGGCCAGGGACTTGGCT
TCAGCAGGAAACCTACTCAAGAAGCATCAGCTATTGGAGAGAGAGATGTTGGCTCGAGAG
GATGCACTCAAGGACCTGAATACATTGGCTGAAGATTTGCTCTCCAGCGGGACTTTCAAC
GTTGATCAGATTGTGAAGAAAAAAGATAATGTCAACAAGCGTTTCCTGAATGTCCAAGAA
TTGGCAGCTGCACACCACGAAAAATTGAAAGAGGCCTATGCCTTGTTCCAGTTCTTCCAG
GATCTAGATGATGAGGAATCCTGGATAGAGGAGAAGTTGATACGAGTGAGCTCCCAGGAC
TATGGGAGAGATCTTCAGGGGGTTCAGAACTTGCTGAAGAAGCACAAACGCCTAGAGGGG
GAGCTGGTGGCCCATGAGCCTGCCATCCAGAATGTGCTGGATATGGCAGAGAAGCTGAAA
GACAAGGCTGCTGTGGGGCAAGAGGAGATCCAGTTGCGGCTGGCTCAGTTTGTTGAACAC
TGGGAGAAGCTCAAAGAGTTGGCCAAGGCCCGAGGACTTAAGTTGGAAGAATCCCTAGAA
TACTTGCAATTCATGCAGAATGCTGAGGAAGAGGAAGCTTGGATCAATGAAAAGAATGCT
TTGGCTGTCCGAGGAGATTGTGGAGATACATTAGCTGCTACTCAGAGCTTGCTAATGAAG
CATGAAGCTTTGGAAAATGACTTTGCTGTCCATGAGACCCGAGTACAAAATGTGTGTGCA
CAAGGAGAAGACATCCTAAATAAGGTGTTGCAGGAGGAAAGTCAGAACAAAGAGATTTCT
TCCAAGATAGAGGCTCTGAATGAAAAGACCCCTTCTCTGGCTAAGGCAATAGCTGCTTGG
AAGTTGCAATTGGAAGACGATTATGCCTTTCAGGAATTCAACTGGAAGGCTGATGTGGTA
GAGGCTTGGATAGCTGATAAGGAAACAAGCCTAAAGACCAATGGCAATGGTGCAGACCTT
GGTGACTTCCTCACTCTTCTGGCAAAACAGGACACTCTGGATGCCAGTCTGCAGAGTTTC
CAGCAAGAGAGACTTCCCGAGATCACTGACCTGAAGGACAAACTGATTTCTGCTCAACAC
AACCAGTCTAAAGCCATTGAAGAGCGTTATGCCGCTCTGCTGAAGCGCTGGGAACAGTTG
CTGGAAGCCTCGGCAGTCCACAGACAGAAATTGCTGGAGAAACAGCTGCCTCTACAGAAG
GCTGAGGACCTGTTCGTGGAATTTGCACATAAGGCTTCAGCTTTGAACAACTGGTGTGAA
AAGATGGAAGAAAACTTGTCAGAGCCTGTGCACTGTGTCTCCCTGAATGAAATTCGGCAG
CTGCAGAAAGACCATGAGGACTTCTTGGCCTCCCTGGCTAGGGCTCAAGCAGACTTTAAA
TGTTTGCTGGAGCTAGACCAGCAGATTAAGGCCTTAGGTGTGCCTTCCAGCCCTTATACC
TGGTTAACAGTGGAGGTGCTGGAAAGGACCTGGAAGCACCTATCTGACATCATTGAGGAA
CGGGAGCAGGAGCTGCAAAAGGAAGAGGCAAGACAGGTCAAGAACTTTGAGATGTGTCAG
GAGTTTGAACAGAATGCCAGTACCTTCCTTCAATGGATCCTGGAAACCAGGGCTTACTTT
CTGGATGGATCATTGCTCAAAGAAACAGGAACTCTGGAATCTCAGCTGGAAGCAAATAAA
AGAAAACAGAAGGAGATCCAGGCGATGAAGCGTCAACTAACCAAGATTGTGGACCTGGGG
GACAACTTGGAAGACGCTCTGATCCTTGATATCAAATACAGCACCATTGGATTGGCTCAG
CAGTGGGACCAGCTCTACCAGCTTGGGTTGCGGATGCAACACAACCTGGAGCAACAGATC
CAGGCCAAGGACATCAAAGGTGTGAGTGAAGAGACTCTAAAGGAATTTAGCACAATCTAT
AAACACTTTGATGAGAATTTGACAGGGCGCCTGACTCACAAAGAGTTCCGGTCCTGCCTG
AGAGGACTCAATTACTACTTGCCCATGGTGGAGGAGGATGAACATGAGCCCAAGTTTGAG
AAGTTCCTGGATGCTGTGGATCCAGGGAGGAAGGGCTATGTCTCACTGGAGGACTATACT
GCTTTCCTGATTGACAAGGAGTCAGAAAACATCAAGTCCAGTGATGAAATAGAGAATGCC
TTCCAAGCCCTGGCAGAGGGCAAGTCATATATTACCAAAGAAGACATGAAGCAGGCCCTT
ACCCCAGAGCAAGTGTCATTCTGTGCCACACATATGCAGCAATATATGGACCCACGGGGT
CGAAGCCATCTCTCTGGCTATGACTACGTTGGCTTCACCAATTCCTACTTTGGCAACTTG
Protein sequence predicted based on nucleotide sequence
MEQFPKETVVESSGPKVLETAEEIQERRQEVLTRYQSFKERVAERGQKLEDSYHLQVFKR DADDLGKWIMEKVNILTDKSYEDPTNIQGKYQKHQSLEAEVQTKSRLMSELEKTREERFT MGHSAHEETKAHIEELRHLWDLLLELTLEKGDQLLRALKFQQYVQECADILEWIGDKEAI ATSVELGEDWERTEVLHKKFEDFQVELVAKEGRVVEVNQYANECAEENHPDLPLIQSKQN EVNAAWERLRGLALQRQKALSNAANLQRFKRDVTEAIQWIKEKEPVLTSEDYGKDLVASE GLFHSHKGLERNLAVMSDKVKELCAKAEKLTLSHPSDAPQIQEMKEDLVSSWEHIRALAT SRYEKLQATYWYHRFSSDFDELSGWMNEKTAAINADELPTDVAGGEVLLDRHQQHKHEID SYDDRFQSADETGQDLVNANHEASDEVREKMEILDNNWTALLELWDERHRQYEQCLDFHL FYRDSEQVDSWMSRQEAFLENEDLGNSLGSAEALLQKHEDFEEAFTAQEEKIITVDKTAT KLIGDDHYDSENIKAIRDGLLARRDALREKAATRRRLLKESLLLQKLYEDSDDLKNWINK KKKLADDEDYKDIQNLKSRVQKQQVFEKELAVNKTQLENIQKTGQEMIEGGHYASDNVTT RLSEVASLWEELLEATKQKGTQLHEANQQLQFENNAEDLQRWLEDVEWQVTSEDYGKGLA EVQNRLRKHGLLESAVAARQDQVDILTDLAAYFEEIGHPDSKDIRARQESLVCRFEALKE PLATRKKKLLDLLHLQLICRDTEDEEAWIQETEPSATSTYLGKDLIASKKLLNRHRVILE NIASHEPRIQEITERGNKMVEEGHFAAEDVASRVKSLNQNMESLRARAARRQNDLEANVQ FQQYLADLHEAETWIREKEPIVDNTNYGADEEAAGALLKKHEAFLLDLNSFGDSMKALRN QANACQQQQAAPVEGVAGEQRVMALYDFQARSPREVTMKKGDVLTLLSSINKDWWKVEAA DHQGIVPAVYVRRLAHDEFPMLPQRRREEPGNITQRQEQIENQYRSLLDRAEERRRRLLQ RYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQKDLNTNEPRLRDINKV ADDLLFEGLLTPEGAQIRQELNSRWGSLQRLADEQRQLLGSAHAVEVFHREADDTKEQIE KKCQALSAADPGSDLFSVQALQRRHEGFERDLVPLGDKVTILGETAERLSESHPDATEDL QRQKMELNEAWEDLQGRTKDRKESLNEAQKFYLFLSKARDLQNWISSIGGMVSSQELAED LTGIEILLERHQEHRADMEAEAPTFQALEDFSAELIDSGHHASPEIEKKLQAVKLERDDL EKAWEKRKKILDQCLELQMFQGNCDQVESWMVARENSLRSDDKSSLDSLEALMKKRDDLD KAITAQEGKITDLEHFAESLIADEHYAKEEIATRLQRVLDRWKALKAQLIDERTKLGDYA NLKQFYRDLEELEEWISEMLPTACDESYKDATNIQRKYLKHQTFAHEVDGRSEQVHGVIN LGNSLIECSACDGNEEAMKEQLEQLKEHWDHLLERTNDKGKKLNEASRQQRFNTSIRDFE FWLSEAETLLAMKDQARDLASAGNLLKKHQLLEREMLAREDALKDLNTLAEDLLSSGTFN VDQIVKKKDNVNKRFLNVQELAAAHHEKLKEAYALFQFFQDLDDEESWIEEKLIRVSSQD YGRDLQGVQNLLKKHKRLEGELVAHEPAIQNVLDMAEKLKDKAAVGQEEIQLRLAQFVEH WEKLKELAKARGLKLEESLEYLQFMQNAEEEEAWINEKNALAVRGDCGDTLAATQSLLMK HEALENDFAVHETRVQNVCAQGEDILNKVLQEESQNKEISSKIEALNEKTPSLAKAIAAW KLQLEDDYAFQEFNWKADVVEAWIADKETSLKTNGNGADLGDFLTLLAKQDTLDASLQSF QQERLPEITDLKDKLISAQHNQSKAIEERYAALLKRWEQLLEASAVHRQKLLEKQLPLQK AEDLFVEFAHKASALNNWCEKMEENLSEPVHCVSLNEIRQLQKDHEDFLASLARAQADFK CLLELDQQIKALGVPSSPYTWLTVEVLERTWKHLSDIIEEREQELQKEEARQVKNFEMCQ EFEQNASTFLQWILETRAYFLDGSLLKETGTLESQLEANKRKQKEIQAMKRQLTKIVDLG DNLEDALILDIKYSTIGLAQQWDQLYQLGLRMQHNLEQQIQAKDIKGVSEETLKEFSTIY KHFDENLTGRLTHKEFRSCLRGLNYYLPMVEEDEHEPKFEKFLDAVDPGRKGYVSLEDYT AFLIDKESENIKSSDEIENAFQALAEGKSYITKEDMKQALTPEQVSFCATHMQQYMDPRG RSHLSGYDYVGFTNSYFGNL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
