Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860010
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5199nts
Open reading frame: 1 to 5199
ATGGCGGCGATCGGCCGCGGCCGCTCTCTGAAGAACCTCCGAGTACGAGGGCGGAATGAC
AGCGGCGAGGAGAACGTCCCGCTGGATCTGACCCGAGAACCTTCTGATAACTTAAGAGAG
ATTCTCCAAAATGTGGCCAGATTGCAGGGAGTATCAAATATGAGAAAGCTAGGCCATCTG
AATAACTTTACTAAGCTTCTTTGTGATATTGGCCACAGTGAAGAAAAACTGGGCTTTCAC
TATGAGGATATCATAATTTGTTTGCGGTTAGCTTTATTAAATGAAGCAAAAGAAGTGCGA
GCAGCAGGGCTACGAGCGCTTCGATATCTCATCCAAGACTCCAGTATTCTCCAGAAGGTG
CTAAAATTGAAAGTGGACTATTTAATAGCTAGGTGCATTGACATACAACAGAGCAACGAG
GTAGAGAGGACACAAGCACTTCGATTAGTCAGAAAGATGATTACTGTGAATGCTTCCTTG
TTTCCTAGTTCTGTGACCAACTCATTAATTGCAGTTGGAAATGATGGACTTCAAGAAAGA
GACAGAATGGTCCGAGCATGCATTGCCATTATCTGTGAACTAGCACTTCAGAATCCAGAG
GTGGTGGCCCTTCGAGGTGGACTAAACACCATCTTGAAAAATGTGATCGATTGCCAATTA
AGTCGAATAAATGAGGCCCTAATTACTACAATTTTGCACCTTCTTAATCATCCAAAGACT
CGACAGTATGTGCGAGCTGATGTAGAATTAGAGAGAATTTTAGCACCCTATACTGATTTT
CACTACAGACATAGTCCAGATACAGCTGAAGGACAGCTCAAAGAAGACAGAGAAGCACGA
TTTCTAGCCAGTAAAATGGGAATCATAGCAACATTCCGATCATGGGCAGGTATTATTAAT
TTATGTAAACCTGGAAATTCTGGGATCCAGTCTCTAATAGGAGTACTTTGCATACCAAAT
ATGGAAATAAGGCGAGGTCTACTTGAAGTGCTTTATGATATATTTCGTCTTCCTCTACCT
GTTGTGACTGAGGAGTTCATAGAAGCACTACTCAGTGTAGATCCAGGGAGGTTCCAAGAC
AGTTGGAGGCTTTCAGATGGCTTTGTGGCAGCTGAGGCAAAAACTATTCTTCCTCATCGT
GCCAGATCCAGGCCAGACCTCATGGATAATTATTTGGCACTGATACTCTCTGCATTTATT
CGTAATGGACTTTTAGAGGGTCTAGTTGAAGTGATAACAAACAGTGATGATCATATCTCA
GTTAGAGCTACCATCCTTTTAGGAGAGCTTTTACATATGGCAAACACAATTCTTCCTCAT
TCACATAGCCATCATTTACACTGCTTGCCAACCCTAATGAATATGGCTGCATCCTTTGAT
ATCCCCAAGGAAAAGAGACTGCGAGCCAGTGCAGCCTTGAACTGTTTAAAACGCTTCCAT
GAAATGAAGAAACGAGGACCTAAGCCTTATAGTCTTCATTTAGACCACATTATTCAGAAA
GCAATTGCAACACACCAGAAACGGGATCAGTATCTCCGAGTTCAGAAAGATATATTTATC
CTTAAGGATACAGAGGAAGCTCTTTTAATTAACCTTAGAGATAGCCAAGTCCTTCAACAT
AAAGAGAATCTTGAATGGAATTGGAATCTTATAGGGACCATTCTTAAGTGGCCAAATGTA
AATCTAAGAAACTATAAAGATGAACAGTTACACAGGTTTGTACGAAGACTACTTTATTTT
TACAAGCCCAGCAGTAAATTATATGCCAACCTGGATCTGGATTTTGCCAAGGCCAAACAG
CTCACGGTTGTAGGTTGCCAGTTTACAGAATTTCTTCTTGAATCTGAAGAGGATGGGCAA
GGCTACTTAGAAGATCTAGTAAAGGATATTGTTCAGTGGCTCAATGCTTCATCTGGAATG
AAACCCGAAAGAAGTCTTCAAAATAATGGTTTATTGACCACCCTTAGTCAACACTACTTT
TTATTTATTGGAACACTTTCTTGCCACCCTCATGGAGTTAAAATGCTGGAAAAATGCAGT
GTATTTCAGTGTCTCCTTAATCTTTGCTCCTTGAAAAACCAAGATCACTTGCTAAAACTT
ACTGTTTCTAGCTTGGACTATAGCAGAGATGGATTGGCTAGAGTCATCCTTTCCAAAATT
TTAACTGCAGCTACTGATGCCTGCAGACTCTATGCAACAAAACATTTAAGGGTATTATTG
AGAGCTAATGTTGAATTCTTTAATAATTGGGGAATTGAGTTGTTAGTGACCCAGCTACAT
GATAAAAACAAAACGATTTCCTCTGAAGCTCTTGATATCCTCGATGAAGCATGTGAAGAC
AAGGCCAATCTTCATGCTCTCATTCAGATGAAACCAGCGTTATCCCACCTTGGAGACAAG
GGTTTGCTTCTCCTGCTGAGATTTCTCTCCATTCCAAAAGGATTTTCCTATCTGAATGAA
AGAGGTTATGTAGCAAAACAATTGGAAAAGTGGCACAGGGAATACAACTCCAAATATGTT
GACTTGATTGAGGAACAACTCAATGAAGCACTTACTACTTACCGGAAGCCTGTTGATGGT
GATAACTATGTTCGTCGGAGTAACCAAAGATTACAGCGTCCTCACGTCTACCTGCCTATA
CACCTTTATGGACAACTAGTACACCATAAAACAGGCTGCCATTTGTTGGAAGTACAGAAT
ATTATTACAGAACTCTGTCGTAATGTTCGTACACCAGATTTGGATAAGTGGGAAGAAATT
AAAAAACTGAAAGCATCTCTTTGGGCCTTGGGAAATATCGGCTCATCAAATTGGGGTCTC
AATTTGCTACAGGAAGAAAACGTGATTCCAGATATACTAAAACTTGCAAAACAGTGTGAA
GTTCTTTCCATCAGAGGGACCTGTGTATATGTACTTGGGCTCATAGCTAAAACCAAACAA
GGCTGTGATATTCTAAAATGTCACAACTGGGATGCTGTGAGGCATAGTCGCAAACATCTG
TGGCCAGTGGTTCCAGATGATGTGGAACAACTCTGTAATGAACTTTCATCTATCCCAAGC
ACTCTAAGTTTGAACTCGGAGTCAACCAGCTCTAGACATAATAGTGAAAGTGAATCTGTG
CCATCGAGTATGTTCATATTGGAGGATGACCGGTTTGGCAGCAGCTCTACTAGTACATTT
TTCCTTGATATCAATGAAGATACAGAGCCAACATTTTATGACCGATCTGGACCCATAAAG
GATAAAAATTCATTCCCTTTCTTTGCTTCTAGTAAACTTGTGAAGAATCGTATCTTAAAT
TCGCTTACTTTGCCTAACAAAAAACATCGTAGTAGCAGTGATCCAAAAGGAGGGAAATTA
TCATCTGAAAGTAAGACAAGCAACAGGCGAATCAGAACACTTACGGAGCCCAGTGTTGAT
TTTAATCATAGTGATGATTTTACACCCATATCCACTGTACAGAAAACATTACAATTAGAG
ACTTCATTTATGGGGAATAAGCACATTGAAGACACTGGTAGTACACCAAGCATTGGAGAA
AATGACTTAAAATTCACCAAGAATTTTGGTACAGAGAATCACAGAGAAAATACAAGCCGA
GAGAGGTTAGTAGTAGAAAGTTCAACGAGCTCACATATGAAGATACGTAGCCAAAGTTTC
AATACAGACACTACAACAAGTGGCATAAGTTCAATGAGCTCAAGTCCTTCACGAGAGACA
GTAGGTGTAGATGCTACAACTATGGACACAGACTGTGGAAGCATGAGTACTGTGGTAAGT
ACTAAAACTATTAAGACAAGCCACTATTTGACGCCACAGTCTAACCATCTGTCTCTCTCC
AAATCAAATTCGGTGTCCCTGGTGCCTCCAGGTTCTTCTCATACGCTTCCTAGAAGAGCA
CAGTCCCTTAAAGCACCCTCTATTGCTACAATTAAAAGTCTAGCAGATTGTAACTTTAGT
TACACAAGTTCTAGAGATGCTTTTGGCTATGCTACACTGAAAAGACTACAGCAACAAAGA
ATGCATCCATCCTTATCTCACTCTGAAGCTTTGGCATCTCCAGCAAAAGATGTGCTATTT
ACTGATACCATCACCATGAAGGCCAACAGTTTTGAGTCCAGATTAACACCAAGCAGGATC
GATTTTAAAAAGAAGCATGTCGGGGGAATCAGGAGCTTAAGACCTACAATAACAAACAAC
CTTTTCAGGTTCATGAAAGCCTTAAGTTATGCATCATTAGATAAAGAAGATTTATTGAGT
CCTATTAATCAAAATACCCTGCAACGATCTTCCTCAGTGCGGTCCATGGTGTCCAGTGCC
ACATATGGGGGTTCAGATGATTACATTGGTCTTGCTCTCCCGGTGGATATAAATGATATA
TTCCAGGTAAAGGATATTCCCTATTTTCAGACAAAAAACATACCACCACATGATGATCGA
GGTGCAAGAGCATTTGCCCATGATGCAGGAGGTCTTCCATCTGGAACTGGAGGTCTTGTA
AAAAATTCTTTTCACTTGCTACGACAGCAGATGAGTCTTACGGAAATAATGAATTCAATC
CATTCAGATGCCTCTCTGTTTTTAGAAAGTACAGAAGACACTGGACTACAGGAACATACA
GATGATAACTGCCTTTATTGTGTCTGTATTGAAATTCTGGGTTTCCAGCCCAGCAACCAA
CTGAGTGCAATATGTAGTCATTCAGACTTTCAAGATATTCCATATTCTGATTGGTGTGAG
CAGACTATCCATAATCCTTTAGAAGTGGTTCCCTCTAAGTTTTCGGGGATTTCTGGATGC
AGTGATGGGGTGTCTCAAGAAGGCTCAGCTAGCAGCACCAAAAGCACAGAATTGTTACTA
GGTGTTAAAACAATTCCAGATGATACACCAATGTGCCGTATACTCCTTCGCAAAGAAGTT
CTAAGATTAGTCATTAATTTGAGTAGTTCAGTTTCAACTAAATGTCATGAGACTGGGCTT
TTAACAATTAAGGAGAAGTATCCTCAAACATTTGATGACATATGCCTTTACTCTGAGGTT
TCCCATTTGCTGTCACACTGCACATTCAGACTTCCGTGTCGGAGGTTCATACAAGAATTA
TTTCAAGATGTACAGTTTCTACAAATGCATGAAGAAGCAGAGGCTGTGTTGGCAACACCA
CCAAAGCAACCTATAGTTGATACATCTGCTGAATCCTTG
Protein sequence predicted based on nucleotide sequence
MAAIGRGRSLKNLRVRGRNDSGEENVPLDLTREPSDNLREILQNVARLQGVSNMRKLGHL NNFTKLLCDIGHSEEKLGFHYEDIIICLRLALLNEAKEVRAAGLRALRYLIQDSSILQKV LKLKVDYLIARCIDIQQSNEVERTQALRLVRKMITVNASLFPSSVTNSLIAVGNDGLQER DRMVRACIAIICELALQNPEVVALRGGLNTILKNVIDCQLSRINEALITTILHLLNHPKT RQYVRADVELERILAPYTDFHYRHSPDTAEGQLKEDREARFLASKMGIIATFRSWAGIIN LCKPGNSGIQSLIGVLCIPNMEIRRGLLEVLYDIFRLPLPVVTEEFIEALLSVDPGRFQD SWRLSDGFVAAEAKTILPHRARSRPDLMDNYLALILSAFIRNGLLEGLVEVITNSDDHIS VRATILLGELLHMANTILPHSHSHHLHCLPTLMNMAASFDIPKEKRLRASAALNCLKRFH EMKKRGPKPYSLHLDHIIQKAIATHQKRDQYLRVQKDIFILKDTEEALLINLRDSQVLQH KENLEWNWNLIGTILKWPNVNLRNYKDEQLHRFVRRLLYFYKPSSKLYANLDLDFAKAKQ LTVVGCQFTEFLLESEEDGQGYLEDLVKDIVQWLNASSGMKPERSLQNNGLLTTLSQHYF LFIGTLSCHPHGVKMLEKCSVFQCLLNLCSLKNQDHLLKLTVSSLDYSRDGLARVILSKI LTAATDACRLYATKHLRVLLRANVEFFNNWGIELLVTQLHDKNKTISSEALDILDEACED KANLHALIQMKPALSHLGDKGLLLLLRFLSIPKGFSYLNERGYVAKQLEKWHREYNSKYV DLIEEQLNEALTTYRKPVDGDNYVRRSNQRLQRPHVYLPIHLYGQLVHHKTGCHLLEVQN IITELCRNVRTPDLDKWEEIKKLKASLWALGNIGSSNWGLNLLQEENVIPDILKLAKQCE VLSIRGTCVYVLGLIAKTKQGCDILKCHNWDAVRHSRKHLWPVVPDDVEQLCNELSSIPS TLSLNSESTSSRHNSESESVPSSMFILEDDRFGSSSTSTFFLDINEDTEPTFYDRSGPIK DKNSFPFFASSKLVKNRILNSLTLPNKKHRSSSDPKGGKLSSESKTSNRRIRTLTEPSVD FNHSDDFTPISTVQKTLQLETSFMGNKHIEDTGSTPSIGENDLKFTKNFGTENHRENTSR ERLVVESSTSSHMKIRSQSFNTDTTTSGISSMSSSPSRETVGVDATTMDTDCGSMSTVVS TKTIKTSHYLTPQSNHLSLSKSNSVSLVPPGSSHTLPRRAQSLKAPSIATIKSLADCNFS YTSSRDAFGYATLKRLQQQRMHPSLSHSEALASPAKDVLFTDTITMKANSFESRLTPSRI DFKKKHVGGIRSLRPTITNNLFRFMKALSYASLDKEDLLSPINQNTLQRSSSVRSMVSSA TYGGSDDYIGLALPVDINDIFQVKDIPYFQTKNIPPHDDRGARAFAHDAGGLPSGTGGLV KNSFHLLRQQMSLTEIMNSIHSDASLFLESTEDTGLQEHTDDNCLYCVCIEILGFQPSNQ LSAICSHSDFQDIPYSDWCEQTIHNPLEVVPSKFSGISGCSDGVSQEGSASSTKSTELLL GVKTIPDDTPMCRILLRKEVLRLVINLSSSVSTKCHETGLLTIKEKYPQTFDDICLYSEV SHLLSHCTFRLPCRRFIQELFQDVQFLQMHEEAEAVLATPPKQPIVDTSAESL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
