Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860024
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4176nts
Open reading frame: 1 to 4176
ATGCCGTCTTCTTTGTTGGGCGCGGCGATGCCGGCCTCTACATCTGCCGCAGCCCTGCAG
GAAGCTCTGGAAAATGCTGGACGGCTCATCGACCGTCAGTTGCAAGAGGACCGCATGTAC
CCGGACCTTTCCGAGCTGCTTATGGTGTCTGCCCCAAATAATCCCACCGTTTCTGGCATG
TCAGATATGGATTATCCTTTGCAAGGACCTGGTTTGCTGTCCGTACCCAACCTTCCAGAG
ATCAGTTCCATCCGAAGAGTTCCTCTCCCACCTGAACTTGTTGAGCAGTTTGGACATATG
CAGTGTAATTGCATGATGGGTGTGTTCCCTCCTATCAGCAGAGCTTGGCTCACAATTGAC
AGTGATATATTCATGTGGAACTATGAGGATGGAGGAGACCTTGCCTATTTTGATGGACTT
AGTGAGACTATTCTTGCTGTGGGGCTTGTGAAGCCAAAAGCAGGCATCTTTCAACCTCAT
GTGCGACACCTCCTGGTTTTGGCGACCCCTGTAGACATAGTAATTCTTGGACTCAGCTAT
GCTAATTTGCAAACAGGTTCTGGAGTTCTTAATGATAGTTTGTCTGGTGGAATGCAGTTG
CTTCCAGATCCTTTATATTCTCTTCCTACTGATAATACTTACCTTTTAACAATAACTTCC
ACTGATAATGGCAGAATTTTCTTGGCTGGAAAGGATGGCTGTTTATATGAAGTAGCCTAC
CAGGCTGAAGCAGGGTGGTTTAGCCAAAGATGTAGGAAAATAAACCACTCAAAGAGCTCA
CTTTCTTTCCTTGTTCCTTCCTTGCTACAATTCACGTTCTCAGAAGATGATCCTATTCTT
CAAATTGCAATTGATAATTCTAGAAATATTTTATATACACGATCTGAGAAAGGAGTAATA
CAGGTGTATGATTTGGGACAAGATGGACAAGGAATGAGCAGAGTTGCCTCTGTGTCACAG
AATGCCATTGTCTCTGCTGCTGGTAACATTGCTAGGACCATCGATCGTTCTGTTTTTAAA
CCAATTGTCCAAATAGCAGTGATTGAAAATTCTGAATCACTGGACTGTCAGTTATTGGCT
GTCACACATGCAGGTGTTAGGTTATATTTTAGCACTTGTCCATTCAGACAGCCATTAGCA
CGGCCTAATACACTGACGCTGGTTCATGTCCGCTTACCTCCTGGATTCTCAGCATCTTCA
ACCGTGGAAAAGCCTTCAAAAGTACATAGAGCTCTTTATAGTAAAGGTATTCTATTGATG
GCAGCCTCAGAAAATGAGGATAATGATATTTTATGGTGTGTCAACCATGATACTTTTCCT
TTCCAAAAGCCAATGATGGAAACCCAGATGACAGCTGGTGTTGATGGTCATTCCTGGGCT
CTTTCTGCGATAGATGAATTGAAAGTAGATAAAATAATTACACCTTTAAACAAGGATCAT
ATTCCAATAACTGATTCACCAGTTGTTGTACAGCAGCACATGTTACCTCCGAAGAAATTT
GTTCTCCTCTCAGCACAGGGGAGCCTTATGTTTCATAAACTTAGACCTGTAGATCAACTG
AGGCATCTACTTGTGAGTAATGTGGGAGGAGATGGAGAAGAGATTGAAAGATTCTTTAAA
TTACATCAGGAAGACCAGGCTTGTGCAACTTGCCTTATTCTTGCTTGCTCCACTGCTGCC
TGTGATAGAGAAGTATCTGCCTGGGCTACTCGGGCTTTCTTTAGGTATGGTGGTGAAGCA
CAGATGAGATTTCCAACCACTCTTCCGCCTCCAAGTAATGTTGGTCCCATCTTGGGGTCT
CCTGTCTATTCTAGTTCTCCTGTTCCTAGTGGTAGTCCCTATCCAAATCCATCCTTTTTG
GGAACACCGTCTCATGGTATACAGCCTCCTGCCATGTCAACTCCAGTGTGTGCTCTGGGA
AACCCAGCAACTCAGGCCACAAATATGAGTTGTGTGACTGGACCAGAGATTGTGTACTCT
GGAAAACACAATGGTATTTGCATTTACTTTTCTCGGATCATGGGAAACATTTGGGATGCA
AGCTTAGTTGTGGAGAGAATATTCAAGAGTGGCAACAGAGAGATCACTGCAATTGAAAGT
AGTGTTCCCTGCCAACTGCTAGAGTCAGTGCTACAAGAACTAAAGGGTTTGCAGGAATTT
CTAGACAGAAACTCCCAGTTTGCAGGAGGACCATTAGGAAATCCAAATACTACTGCTAAA
GTGCAGCAGAGGCTGATAGGATTCATGCGTCCTGAAAACGGAAATCCCCAGCAAATGCAA
CAGGAACTGCAGAGGAAGTTTCATGAGGCTCAACTAAGTGAAAAGATTTCACTTCAGGCA
ATTCAGCAGTTGGTTCGAAAATCATATCAGGCTCTGGCTTTATGGAAACTTCTTTGTGAA
CATCAATTCACTATCATTGTGGCAGAACTTCAGAAGGAACTTCAAGAGCAGCTGAAGATC
ACCACCTTTAAAGATCTTGTAATCAGGGACAAAGAACTCACAGGGGCATTAATTGCTTCT
CTTATCAACTGCTACATCAGAGATAATGCCGCTGTTGATGGCATTAGTTTACATTTACAG
GATATCTGCCCACTTCTATATAGCACTGATGATGCAATTTGTTCTAAGGCAAATGAGCTT
CTCCAGCGTTCCCGACAAGTTCAAAATAAGACTGAAAAAGAAAGAATGTTAAGGGAATCA
TTAAAGGAATATCAAAAAATTAGCAATCAAGTGGACCTTTCCAATGTTTGTGCTCAGTAT
AGACAAGTGAGATTTTATGAGGGTGTGGTGGAACTTTCTCTTACGGCTGCAGAGAAAAAA
GATCCTCAAGGTCTTGGGCTTCATTTCTATAAACATGGAGAACCAGAAGAAGACATAGTT
GGACTTCAGGCCTTCCAAGAAAGATTAAACAGTTACAAATGCATTACAGACACACTTCAA
GAACTGGTAAATCAAAGTAAGGCCGCTCCTCAGTCTCCCAGTGTACCCAAAAAACCTGGT
CCTCCAGTGTTGTCATCTGATCCAAATATGCTGAGTAATGAAGAAGCAGGACATCATTTT
GAACAAATGCTTAAATTGTCACAGCGATCCAAGGATGAGCTCTTTAGTATTGCCCTTTAT
AATTGGCTAATACAAGTCGACCTTGCAGATAAGCTGCTACAGGTTGCTTCTCCATTTCTG
GAGCCACATCTAGTCCGAATGGCCAAAGTTGATCAAAACAGAGTTCGTTATATGGATTTA
CTCTGGCGGTATTACGAGAAGAACAGAAGTTTCAGTAATGCTGCTCGTGTACTGTCCAGA
CTGGCTGACATGCATAGCACAGAAATTTCACTTCAGCAGCGACTAGAGTACATTGCTCGA
GCCATTCTTAGTGCCAAAAGTTCCACTGCCATTTCATCAATAGCTGCCGATGGTGAATTT
CTTCATGAATTAGAAGAAAAAATGGAGGTTGCTAGGATCCAACTTCAGATACAGGAGACA
CTACAAAGGCAGTATTCCCATCATTCTTCTGTACAGGATGCAGTTTCTCAGCTGGATTCT
GAGCTGATGGACATAACTAAGCTTTATGGGGAATTTGCTGACCCATTTAAACTTGCAGAG
TGCAAACTTGCAATAATTCATTGTGCCGGTTATTCAGACCCTATATTGGTGCAGACACTT
TGGCAAGATATCATAGAGAAAGAATTGAGTGACAGTGTGACATTGAGCTCCTCGGATAGA
ATGCATGCTCTTAGTCTCAAGATTGTTCTCCTTGGCAAAATTTATGCTGGCACACCACGC
TTCTTTCCTTTAGATTTTATTGTACAGTTTTTAGAACAGCAGGTTTGTACTTTGAACTGG
GATGTGGGCTTCGTAATACAGACAATGAATGAAATTGGAGTACCATTACCTAGACTACTA
GAAGTTTATGATCAGTTGTTCAAATCACGGGATCCATTCTGGAACAGAATGAAGAAGCCA
CTGCACCTTTTGGATTGTATACATGTATTATTGATAAGATATGTTGAGAATCCCAGCCAA
GTTTTAAATTGTGAAAGGAGAAGATTTACAAATCTCTGCCTGGATGCTGTTTGTGGTTAC
CTTGTTGAGCTCCAGTCTATGAGCTCGTCAGTAGCAGTACAAGCCATCACTGGGAATTTT
AAATCTCTTCAAGCTAAATTAGAACGGCTTCATTTG
Protein sequence predicted based on nucleotide sequence
MPSSLLGAAMPASTSAAALQEALENAGRLIDRQLQEDRMYPDLSELLMVSAPNNPTVSGM SDMDYPLQGPGLLSVPNLPEISSIRRVPLPPELVEQFGHMQCNCMMGVFPPISRAWLTID SDIFMWNYEDGGDLAYFDGLSETILAVGLVKPKAGIFQPHVRHLLVLATPVDIVILGLSY ANLQTGSGVLNDSLSGGMQLLPDPLYSLPTDNTYLLTITSTDNGRIFLAGKDGCLYEVAY QAEAGWFSQRCRKINHSKSSLSFLVPSLLQFTFSEDDPILQIAIDNSRNILYTRSEKGVI QVYDLGQDGQGMSRVASVSQNAIVSAAGNIARTIDRSVFKPIVQIAVIENSESLDCQLLA VTHAGVRLYFSTCPFRQPLARPNTLTLVHVRLPPGFSASSTVEKPSKVHRALYSKGILLM AASENEDNDILWCVNHDTFPFQKPMMETQMTAGVDGHSWALSAIDELKVDKIITPLNKDH IPITDSPVVVQQHMLPPKKFVLLSAQGSLMFHKLRPVDQLRHLLVSNVGGDGEEIERFFK LHQEDQACATCLILACSTAACDREVSAWATRAFFRYGGEAQMRFPTTLPPPSNVGPILGS PVYSSSPVPSGSPYPNPSFLGTPSHGIQPPAMSTPVCALGNPATQATNMSCVTGPEIVYS GKHNGICIYFSRIMGNIWDASLVVERIFKSGNREITAIESSVPCQLLESVLQELKGLQEF LDRNSQFAGGPLGNPNTTAKVQQRLIGFMRPENGNPQQMQQELQRKFHEAQLSEKISLQA IQQLVRKSYQALALWKLLCEHQFTIIVAELQKELQEQLKITTFKDLVIRDKELTGALIAS LINCYIRDNAAVDGISLHLQDICPLLYSTDDAICSKANELLQRSRQVQNKTEKERMLRES LKEYQKISNQVDLSNVCAQYRQVRFYEGVVELSLTAAEKKDPQGLGLHFYKHGEPEEDIV GLQAFQERLNSYKCITDTLQELVNQSKAAPQSPSVPKKPGPPVLSSDPNMLSNEEAGHHF EQMLKLSQRSKDELFSIALYNWLIQVDLADKLLQVASPFLEPHLVRMAKVDQNRVRYMDL LWRYYEKNRSFSNAARVLSRLADMHSTEISLQQRLEYIARAILSAKSSTAISSIAADGEF LHELEEKMEVARIQLQIQETLQRQYSHHSSVQDAVSQLDSELMDITKLYGEFADPFKLAE CKLAIIHCAGYSDPILVQTLWQDIIEKELSDSVTLSSSDRMHALSLKIVLLGKIYAGTPR FFPLDFIVQFLEQQVCTLNWDVGFVIQTMNEIGVPLPRLLEVYDQLFKSRDPFWNRMKKP LHLLDCIHVLLIRYVENPSQVLNCERRRFTNLCLDAVCGYLVELQSMSSSVAVQAITGNF KSLQAKLERLHL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
