Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860025
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3627nts
Open reading frame: 1 to 3627
ATGGACTTGCACATGATGAACTGTGAACTTCTAGCCACATGTAGTGCCCTTGGGTACTTG
GAGGGAGACACTTACCATAAGGAACCAGATTGCTTAGAGAGCGTGAAGGATCTGATCCGC
TATTTGAGGCATGAGGATGAGACACGAGATGTGCGGCAGCAGCTGGGGGCAGCCCAGATC
CTACAGAGCGACCTTCTGCCCATCCTCACCCAGCACCACCAGGACAAGCCTCTCTTTGAT
GCTGTTATCAGACTGATGGTGAACTTGACACAACCAGCCTTGCTCTGTTTTGGCAATCTG
CCTAAGGAGCCCAGCTTTCGGCACCATTTTTTGCAGGTGCTAACTTATTTGCAGGCCTAC
AAAGAGGCCTTTGCCAGTGAGAAGGCTTTTGGAGTCCTCAGTGAAACCTTGTATGAGCTG
CTGCAGCTGGGCTGGGAGGAACGGCAGGAGGAAGACAACTTGCTGATTGAACGGATCCTA
CTGCTGGTCAGAAATATTCTCCATGTCCCAGCTGACCTTGATCAGGAGAAGAAGATTGAT
GATGACGCCAGTGCCCATGACCAGCTCCTCTGGGCGATTCACCTCAGCGGCCTGGATGAC
CTGCTCCTCTTTCTGGCCAGCTCGTCTGCTGAGGAGCAATGGAGCCTACATGTGCTAGAG
ATTGTCTCCCTTATGTTTCGTGACCAGAACCCCGAGCAGCTGGCGGGAGTAGGGCAGGGA
CGCTTAGCTCAGGAGCGGAGTGCAGATTTTGCAGAACTGGAGGTGTTGCGCCAGCGAGAG
ATGGCAGAAAAGAAGACTCGAGCCCTCCAGCGAGGCAACAGGCATTCTCGATTTGGGGGC
TCCTATATTGTCCAGGGGTTGAAATCCATTGGGGAGAGGGACCTCATCTTTCACAAAGGC
CTTCACAACCTACGAAACTACAGTTCAGATTTGGGAAAGCAGCCGAAAAAGGTGCCTAAA
CGTCGCCAGGCCGCCCGAGAGCTGTCCATTCAGCGCCGTTCTGCCCTCAATGTGAGGCTC
TTCCTCAGAGACTTCTGCTCTGAGTTCCTGGAGAACTGTTACAACCGGCTCATGGGATCA
GTAAAGGATCACCTGCTTCGGGAGAAAGCTCAGCAGCATGATGAGACCTATTATATGTGG
GCCTTGGCTTTCTTCATGGCCTTCAACCGAGCTGCCTCCTTCCGGCCAGGCCTGGTTTCT
GAGACCCTCAGTGTCCGTACCTTCCACTTCATTGAGCAGAACCTCACCAACTACTATGAG
ATGATGCTGACTGACCGCAAGGAAGCTGCCTCCTGGGCACGCCGGATGCACTTGGCTCTG
AAGGCCTATCAGGAGCTGCTGGCAACAGTGAATGAGATGGACATATCTCCAGATGAGGCT
GTGAGGGAGAGCAGCCGCATCATCAAGAACAATATTTTCTATGTGATGGAGTACCGAGAA
CTATTCCTGGCACTTTTTCGAAAGTTTGATGAGAGATGCCAGCCCCGCTCTTTCCTTCGT
GACCTGGTGGAGACCACCCACCTCTTCCTCAAAATGTTGGAGCGATTCTGTCGGAGCCGT
GGGAACCTGGTGGTGCAGAACAAACAAAAGAAGAGAAGGAAGAAGAAGAAGAAGGTCCTA
GACCAGGCCATTGTTTCTGGTAATGTCCCATCTAGCCCAGAAGAAGTGGAGGCTGTGTGG
CCAGCCCTGGCTGAGCAGCTACAGTGCTGTGCCCAGAATTCTGAGCTCAGCATGGACTCC
GTGGTTCCCTTTGATGCGGCCTCAGAGGTGCCAGTGGAGGAGCAGCGGGCAGAAGCTATG
GTACGGATCCAAGACTGTCTCCTGGCTGGCCAGGCCCCACAGGCCCTGACTCTCCTGAGG
TCTGCTCGGGAGGTGTGGCCTGAAGGAGATGTGTTTGGCTCTCAAGACATTTCTCCAGAG
GAAGAGATCCAGTTGCTGAAACAAATCCTCTCTGCTCCACTTCCCCGGCAGCAGGGCCCA
GAGGAACGTGGGGCAGAGGAAGAAGAAGAGGAGGAGGAGGAGGAAGAGGAGGAGTTGCAA
GTGGTCCAGGTGTCGGAGAAAGAATTTAATTTTCTGGACTACCTGAAACGCTTTGCATGT
TCAACTGTCGTTCGAGCCTATGTGCTGCTACTAAGGAGCTACCAGCAGAATAGTGCCCAC
ACTAACCATTGCATTGTGAAGATGCTGCACCGGCTGGCCCATGACCTCAAAATGGAAGCC
CTACTTTTTCAGCTGTCAGTCTTCTGCCTCTTCAATCGTCTGCTTAGTGACCCTGCTGCT
GGAGCCTACAAAGAGCTAGTGACTTTTGCCAAATACATCCTGGGCAAATTTTTTGCACTG
GCTGCAGTCAACCAAAAAGCCTTTGTGGAGCTGTTGTTCTGGAAGAACACAGCTGTGGTT
CGAGAGATGACTGAGGGCTATGGCTCCCTGGATGACAGGTCTTCCAGTCGCAGAGCACCT
ACATGGAGCCCCGAAGAAGAGGCTCATCTTCGGGAGCTGTACCTCGCCAATAAGGACGTG
GAAGGGCAGGATGTGGTGGAAGCCATCTTGGCCCACCTGAATACTGTTCCTCGAACACGC
AAGCAGATCATCCACCATCTGGTACAGATGGGACTGGCTGACAGTGTCAAGGACTTCCAA
AGGAAAGGAACCCATATTGTACTGTGGACGGGGGATCAGGAGTTGGAGCTGCAGCGGCTT
TTTGAGGAATTCCGGGACTCAGATGATGTCCTGGGTCATATCATGAAGAATATCACAGCC
AAACGCTCACGGGCCCGAATAGTGGATAAACTCTTGGCTCTGGGGCTGGTGGCTGAGCGG
CGGGAGCTGTACAAGAAACGGCAGAAAAAGTTGGCATCCTCCATCTTGCCAAATGGAGCG
GAGTCCCTGAAAGATTTTTGCCAGGAAGATCTGGAAGAAGAGGAAAACCTGCCTGAGGAA
GACAGCGAAGAGGAAGAAGAAGGGGGCTCAGAAGCAGAACAAGTCCAGGGTAGCTTAGTC
CTTTCAAATGAAAACCTTGGTCAAAGCCTGCATCAGGAAGGCTTTTCTATCCCGCTCCTA
TGGCTCCAGAACTGCCTGATCCGAGCAGCTGATGATCGGGAAGAGGATGGCTGCTCCCAG
GCCGTTCCATTGGTGCCACTCACAGAGGAAAATGAGGAAGCCATGGAAAACGAACAGTTT
CAGCAGCTGTTGCGCAAGCTAGGGGTTCGGCCCCCTGCCTCTGGGCAGGAAACCTTCTGG
CGAATTCCAGCCAAGCTGAGTCCTACCCAGCTCCGGAGGGCAGCAGCTTCTTTGAGTCAA
CCAGAGGAGGAACAGAAGCTGCAGCCAGAGCTGCAGCCTAAAGTCCCTGGAGAGCAAGGC
TCTGATGAGGAGCACTGTAAAGAGCACCGAGCACAAGCCCTGAGGGCCCTCTTGCTAGCC
CACAAGAAGAAAGCGGGCCTGGCATCCCCAGAGGAGGAAGACGCTGTTGGTAAAGAGCCG
CTGAAGGCAGCACCCAAGAAACGACAATTGCTGGACAGCGACGAGGAACAGGAAGAAGAT
GAGGGCAGGAACAGAGCACCAGAGTTGGGAGCTCCAGGAATCCAAAAGAAGAAACGATAC
CAGATTGAGGATGATGAGGATGACTTG
Protein sequence predicted based on nucleotide sequence
MDLHMMNCELLATCSALGYLEGDTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQI LQSDLLPILTQHHQDKPLFDAVIRLMVNLTQPALLCFGNLPKEPSFRHHFLQVLTYLQAY KEAFASEKAFGVLSETLYELLQLGWEERQEEDNLLIERILLLVRNILHVPADLDQEKKID DDASAHDQLLWAIHLSGLDDLLLFLASSSAEEQWSLHVLEIVSLMFRDQNPEQLAGVGQG RLAQERSADFAELEVLRQREMAEKKTRALQRGNRHSRFGGSYIVQGLKSIGERDLIFHKG LHNLRNYSSDLGKQPKKVPKRRQAARELSIQRRSALNVRLFLRDFCSEFLENCYNRLMGS VKDHLLREKAQQHDETYYMWALAFFMAFNRAASFRPGLVSETLSVRTFHFIEQNLTNYYE MMLTDRKEAASWARRMHLALKAYQELLATVNEMDISPDEAVRESSRIIKNNIFYVMEYRE LFLALFRKFDERCQPRSFLRDLVETTHLFLKMLERFCRSRGNLVVQNKQKKRRKKKKKVL DQAIVSGNVPSSPEEVEAVWPALAEQLQCCAQNSELSMDSVVPFDAASEVPVEEQRAEAM VRIQDCLLAGQAPQALTLLRSAREVWPEGDVFGSQDISPEEEIQLLKQILSAPLPRQQGP EERGAEEEEEEEEEEEEELQVVQVSEKEFNFLDYLKRFACSTVVRAYVLLLRSYQQNSAH TNHCIVKMLHRLAHDLKMEALLFQLSVFCLFNRLLSDPAAGAYKELVTFAKYILGKFFAL AAVNQKAFVELLFWKNTAVVREMTEGYGSLDDRSSSRRAPTWSPEEEAHLRELYLANKDV EGQDVVEAILAHLNTVPRTRKQIIHHLVQMGLADSVKDFQRKGTHIVLWTGDQELELQRL FEEFRDSDDVLGHIMKNITAKRSRARIVDKLLALGLVAERRELYKKRQKKLASSILPNGA ESLKDFCQEDLEEEENLPEEDSEEEEEGGSEAEQVQGSLVLSNENLGQSLHQEGFSIPLL WLQNCLIRAADDREEDGCSQAVPLVPLTEENEEAMENEQFQQLLRKLGVRPPASGQETFW RIPAKLSPTQLRRAAASLSQPEEEQKLQPELQPKVPGEQGSDEEHCKEHRAQALRALLLA HKKKAGLASPEEEDAVGKEPLKAAPKKRQLLDSDEEQEEDEGRNRAPELGAPGIQKKKRY QIEDDEDDL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
