Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860048
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6099nts
Open reading frame: 1 to 6099
ATGGGAGATGACAGTGAGTGGTTGAAACTGCCAGTTGATCAGAAATGTGAACACAAGCTG
TGGAAAGCAAGGTTAAGTGGGTATGAAGAGGCCCTGAAGATCTTCCAGAAAATAAAGGAT
GAAAAGAGCCCAGAGTGGTCCAAATTTTTAGGATTGATCAAAAAATTTGTCACTGATTCC
AATGCAGTGGTTCAATTGAAAGGATTAGAAGCTGCACTTGTTTATGTTGAAAATGCCCAT
GTAGCAGGAAAAACCACAGGAGAAGTTGTGTCAGGTGTTGTAAGTAAGGTGTTCAATCAA
CCTAAAGCTAAAGCCAAGGAGCTGGGCATAGAGATCTGTCTTATGTACATAGAGATTGAG
AAAGGAGAGGCTGTTCAAGAAGAGCTCCTGAAAGGCTTGGACAATAAGAATCCCAAGATC
ATAGTGGCCTGTATAGAGACACTGAGGAAAGCCTTAAGTGAATTTGGTTCCAAAATCATC
TTGCTTAAGCCAATTATCAAAGTGTTGCCAAAACTCTTTGAGTCTCGAGAGAAGGCTGTT
CGAGATGAAGCCAAACTAATTGCTGTGGAGATTTACAGATGGATTCGGGATGCTCTGAGA
CCCCCATTACAAAATATAAACTCTGTTCAGTTGAAAGAACTAGAAGAAGAATGGGTCAAA
CTGCCAACAAGTGCTCCTAGACCTACTCGATTTCTTCGTTCCCAACAAGAACTAGAAGCT
AAATTGGAACAACAACAGTCTGCTGGTGGAGATGCTGAAGGAGGTGGTGATGATGGTGAT
GAGGTGCCACAAATAGATGCTTATGAGCTTTTAGAAGCTGTAGAAATCCTTTCCAAACTT
CCCAAAGACTTTTATGACAAAATTGAGGCAAAAAAATGGCAAGAGAGAAAAGAGGCCCTG
GAGTCTGTAGAAGTACTAATAAAAAACCCCAAACTGGAAGCTGGCGATTATGCAGATTTA
GTAAAAGCATTAAAGAAGGTTGTTGGAAAGGACACCAATGTCATGTTGGTGGCTTTGGCA
GCAAAATGTCTTACTGGCCTGGCTGTTGGGCTAAGGAAGAAATTTGGACAATATGCAGGA
CATGTTGTGCCAACCATCTTGGAGAAATTCAAAGAGAAGAAACCTCAAGTGGTACAAGCC
CTGCAGGAGGCAATTGATGCAATCTTCCTTACTACCACACTACAGAACATCAGTGAGGAT
GTTTTAGCAGTAATGGATAATAAAAATCCAACCATCAAGCAGCAGACATCTCTTTTTATT
GCAAGAAGTTTCCGCCACTGCACTGCTTCTACCCTGCCAAAGAGCTTGCTAAAGCCCTTT
TGTGCTGCACTACTTAAGCACATCAATGATTCTGCTCCTGAAGTCAGAGATGCCGCATTT
GAAGCATTGGGTACTGCTTTGAAGGTGGTTGGCGAGAAAGCAGTAAACCCATTCCTAGCT
GATGTGGACAAACTCAAGCTTGATAAGATCAAAGAATGTTCAGAAAAGGTAGAACTGATA
CATGGTAAGAAAGCTGGACTAGCTGCTGATAAGAAGGAATTCAAACCTCTGCCTGGAAGG
ACTGCTGCTTCAGGGGCTGCAGGAGATAAGGACACAAAGGACATTTCTGCACCCAAACCA
GGACCTCTAAAAAAGGCACCTGCTGCTAAGGCTGGTGGGCCACCAAAAAAGGGGAAACCA
GCTGCACCAGGAGGCGCAGGGAATACTGGAACCAAGAACAAGAAAGGACTGGAGACTAAA
GAAATAGTGGAGCCTGAGCTCTCGATAGAAGTATGTGAAGAAAAAGCTTCAGCTGTTCTT
CCCCCTACCTGTATACAGCTTCTTGACAGCAGTAACTGGAAAGAAAGGCTGGCTTGTATG
GAAGAGTTCCAGAAGGCTGTTGAGCTAATGGACCGAACTGAAATGCCATGCCAGGCATTA
GTGAGGATGCTAGCCAAGAAACCTGGATGGAAAGAAACTAATTTTCAGGTGATGCAAATG
AAGCTTCATATAGTTGCTTTGATTGCCCAGAAGGGAAATTTTTCCAAAACGTCAGCTCAG
GTTGTATTAGATGGCCTTGTGGACAAGATTGGAGATGTGAAATGTGGGAACAATGCAAAA
GAAGCTATGACAGCAATAGCCGAAGCCTGTATGTTACCATGGACTGCTGAACAGGTTGTG
TCAATGGCTTTCTCACAAAAGAATCCCAAAAATCAGTCAGAAACTCTGAATTGGCTATCA
AATGCCATAAAAGAATTTGGTTTTTCTGGGTTGAATGTCAAAGCTTTCATTAGCAATGTG
AAGACAGCTCTTGCTGCAACAAACCCAGCTGTGAGGACTGCTGCCATAACCCTGCTTGGC
GTGATGTATCTGTATGTTGGTCCCTCTTTGCGAATGTTCTTTGAGGATGAGAAGCCTGCC
CTCCTATCCCAGATAGATGCAGAATTTGAGAAGATGCAGGGACAAAGTCCACCTGCTCCA
ACCAGAGGAATTTCCAAGCATAGCACAAGTGGTACAGATGAAGGAGAAGATGGAGATGAA
CCAGATGACGGGAGCAATGATGTCGTTGATCTTTTGCCGAGGACGGAGATCAGTGATAAA
ATCACTTCAGAGTTGGTATCTAAGATTGGTGATAAGAATTGGAAGATTAGGAAAGAAGGC
CTAGATGAAGTGGCAGGTATTATTAATGACGCAAAATTTATCCAACCGAATATAGGTGAA
CTTCCAACTGCCTTGAAGGGTCGACTCAATGATTCAAATAAAATCTTGGTACAGCAAACG
CTGAATATCCTGCAACAACTGGCAGTAGCCATGGGCCCAAATATTAAGCAACATGTAAAA
AATTTAGGCATCCCTATCATCACAGTCCTTGGAGACAGCAAGAACAATGTTCGAGCTGCT
GCCCTAGCGACTGTGAATGCTTGGGCAGAACAGACTGGCATGAAGGAATGGCTGGAAGGA
GAAGATCTTTCTGAAGAGCTCAAAAAGGAAAATCCTTTCTTGAGGCAAGAGCTTCTGGGC
TGGCTGGCTGAGAAACTACCTACTCTTCGTTCCACCCCTACAGACCTTATCCTTTGTGTT
CCTCATCTCTACTCCTGCCTAGAAGATCGAAATGGAGATGTGCGAAAGAAGGCCCAAGAT
GCCTTGCCATTCTTCATGATGCATTTAGGATATGAAAAAATGGCCAAGGCTACTGGGAAA
CTAAAGCCAACTTCTAAAGATCAGGTATTGGCCATGCTAGAGAAAGCCAAAGTTAACATG
CCAGCCAAGCCTGCTCCACCCACTAAAGCAACTTCTAAACCAATGGGAGGGTCCGCTCCA
GCCAAATTCCAGCCTGCATCAGCACCTGCTGAAGATTGTATTTCCAGCAGTACAGAACCC
AAACCTGATCCAAAAAAGGCCAAAGCTCCAGGATTATCCTCTAAAGCAAAGAGTGCACAA
GGGAAGAAGATGCCAAGCAAAACCAGCTTAAAGGAGGATGAAGACAAATCCGGGCCTATT
TTTATTGTTGTTCCAAATGGAAAAGAGCAAAGGATGAAAGATGAAAAAGGATTGAAGGTG
CTAAAGTGGAATTTTACTACCCCACGGGATGAATACATTGAGCAACTAAAGACTCAAATG
TCTAGCTGTGTGGCTAAATGGTTACAAGATGAGATGTTTCACTCAGACTTTCAGCATCAT
AACAAAGCCCTTGCTGTTATGGTTGATCACTTGGAGAGTGAAAAAGAAGGAGTTATTGGT
TGCCTGGATCTTATCTTAAAGTGGCTTACCCTGAGGTTTTTTGACACCAATACAAGCGTC
CTGATGAAAGCACTAGAATATTTAAAATTGCTCTTCACCTTGCTAAGTGAAGAAGAATAT
CATCTTACTGAGAATGAAGCATCTTCCTTCATCCCCTATCTTGTCGTCAAGGTTGGAGAA
CCAAAGGATGTCATTCGTAAAGATGTTCGTGCCATCCTGAACCGGATGTGCCTTGTCTAC
CCAGCTAGCAAGATGTTTCCCTTTATCATGGAAGGAACCAAATCCAAAAACTCTAAGCAG
AGAGCAGAGTGCCTGGAAGAGCTGGGATGTCTGGTTGAGTCCTATGGCATGAATGTTTGC
CAACCAACCCCAGGAAAAGCCTTAAAGGAAATAGCTGTTCACATAGGAGACCGTGACAAT
GCTGTACGCAATGCTGCACTCAACACCATTGTAACGGTGTACAATGTACATGGGGATCAG
GTGTTCAAACTGATTGGAAATCTTTCTGAAAAGGATATGAGCATGCTCGAGGAGAGGATT
AAGCGGTCAGCAAAGAGACCCTCTGCTGCACCAATAAAACAGGTGGAAGAGAAACCTCAG
CGTGCACAGAACATAAGCTCCAATGCCAACATGTTACGCAAGGGACCAGCTGAGGACATG
TCTTCCAAACTCAACCAAGCCCGAAGCATGAGTGGGCATCCTGAGGCAGCCCAGATGGTC
CGCCGAGAATTCCAGCTGGATCTAGATGAGATTGAGAATGACAATGGTACAGTCCGATGT
GAAATGCCAGAACTTGTTCAGCACAAACTGGATGACATTTTTGAGCCAGTCCTTATTCCT
GAACCCAAGATCCGGGCTGTTTCTCCACACTTCGATGACATGCACAGTAATACAGCATCC
ACAATCAATTTCATTATCTCCCAAGTAGCCAGTGGTGACATCAACACAAGTATCCAAGCT
CTGACACAGATCGATGAGGTCCTGAGACAGGAAGACAAAGCTGAAGCCATGTCCGGCCAT
ATTGATCAGTTTCTGATAGCCACTTTTATGCAGCTAAGACTCATCTACAACACACACATG
GCAGATGAGAAATTGGAGAAGGACGAGATCATCAAGTTGTATAGCTGTATCATTGGCAAC
ATGATTTCGCTGTTTCAGATAGAGAGCCTTGCCCGGGAGGCCTCCACTGGAGTACTAAAA
GACCTAATGCATGGCCTCATCACCTTAATGCTGGATTCTCGGATTGAAGATCTTGAGGAA
GGACAACAGGTCATCCGCTCTGTGAACCTCTTGGTGGTGAAGGTTCTGGAGAAGTCAGAC
CAGACCAACATCCTGAGTGCCCTACTTGTTTTGCTCCAAGACAGCCTGCTAGCAACAGCC
AGTTCTCCCAAATTCTCAGAGCTTGTTATGAAGTGTCTCTGGAGAATGGTTCGACTGTTG
CCTGATACCATCAATAGCATTAACCTAGACAGAATTCTTCTGGATATCCACATTTTCATG
AAGGTCTTCCCCAAAGAGAAACTGAAGCAATGCAAAAGTGAATTTCCCATAAGGACCCTA
AAGACCCTGCTACACACCTTATGCAAATTAAAAGGGCCCAAGATCCTGGACCACCTAACG
ATGATCGACAACAAAAACGAGTCTGAGCTGGAGGCCCATCTCTGCCGGATGATGAAGCAC
AGTATGGACCAGACTGGGAGCAAGTCTGATAAGGAAACAGAAAAGGGAGCATCTCGAATA
GATGAAAAATCATCAAAGGCCAAAGTGAATGATTTCTTAGCTGAGATTTTTAAGAAGATT
GGCTCTAAAGAAAACACTAAAGAGGGACTAGCAGAGTTATATGAATATAAGAAGAAATAC
TCAGATGCTGACATTGAACCATTTCTGAAAAATTCCTCACAGTTCTTCCAGAGCTATGTC
GAAAGAGGCCTTCGGGTGATTGAGATGGAGAGGGAGGGCAAAGGTCGTATTTCCACTTCA
ACAGGCATCTCCCCTCAGATGGAAGTCACATGTGTGCCCACGCCCACAAGCACAGTGTCC
TCCATAGGTAACACAAATGGGGAAGAAGTGGGGCCATCTGTCTACTTGGAAAGGCTAAAG
ATCCTCCGACAGCGATGTGGTCTGGACAACACAAAGCAAGATGACCGACCTCCTTTGACC
TCTTTGCTCTCCAAACCAGCAGTTCCTACTGTCGCCTCTTCCACAGACATGCTCCACAGC
AAACTCTCTCAGCTCCGGGAGTCACGGGAGCAGCACCAGCATTCAGACCTGGATTCTAAC
CAGACTCACTCTTCAGGAACTGTGACCTCCTCCTCCTCCACAGCTAACATAGACGACTTG
AAAAAAAGACTGGAGAGAATAAAGAGCAGTCGCAAATTG
Protein sequence predicted based on nucleotide sequence
MGDDSEWLKLPVDQKCEHKLWKARLSGYEEALKIFQKIKDEKSPEWSKFLGLIKKFVTDS NAVVQLKGLEAALVYVENAHVAGKTTGEVVSGVVSKVFNQPKAKAKELGIEICLMYIEIE KGEAVQEELLKGLDNKNPKIIVACIETLRKALSEFGSKIILLKPIIKVLPKLFESREKAV RDEAKLIAVEIYRWIRDALRPPLQNINSVQLKELEEEWVKLPTSAPRPTRFLRSQQELEA KLEQQQSAGGDAEGGGDDGDEVPQIDAYELLEAVEILSKLPKDFYDKIEAKKWQERKEAL ESVEVLIKNPKLEAGDYADLVKALKKVVGKDTNVMLVALAAKCLTGLAVGLRKKFGQYAG HVVPTILEKFKEKKPQVVQALQEAIDAIFLTTTLQNISEDVLAVMDNKNPTIKQQTSLFI ARSFRHCTASTLPKSLLKPFCAALLKHINDSAPEVRDAAFEALGTALKVVGEKAVNPFLA DVDKLKLDKIKECSEKVELIHGKKAGLAADKKEFKPLPGRTAASGAAGDKDTKDISAPKP GPLKKAPAAKAGGPPKKGKPAAPGGAGNTGTKNKKGLETKEIVEPELSIEVCEEKASAVL PPTCIQLLDSSNWKERLACMEEFQKAVELMDRTEMPCQALVRMLAKKPGWKETNFQVMQM KLHIVALIAQKGNFSKTSAQVVLDGLVDKIGDVKCGNNAKEAMTAIAEACMLPWTAEQVV SMAFSQKNPKNQSETLNWLSNAIKEFGFSGLNVKAFISNVKTALAATNPAVRTAAITLLG VMYLYVGPSLRMFFEDEKPALLSQIDAEFEKMQGQSPPAPTRGISKHSTSGTDEGEDGDE PDDGSNDVVDLLPRTEISDKITSELVSKIGDKNWKIRKEGLDEVAGIINDAKFIQPNIGE LPTALKGRLNDSNKILVQQTLNILQQLAVAMGPNIKQHVKNLGIPIITVLGDSKNNVRAA ALATVNAWAEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLAEKLPTLRSTPTDLILCV PHLYSCLEDRNGDVRKKAQDALPFFMMHLGYEKMAKATGKLKPTSKDQVLAMLEKAKVNM PAKPAPPTKATSKPMGGSAPAKFQPASAPAEDCISSSTEPKPDPKKAKAPGLSSKAKSAQ GKKMPSKTSLKEDEDKSGPIFIVVPNGKEQRMKDEKGLKVLKWNFTTPRDEYIEQLKTQM SSCVAKWLQDEMFHSDFQHHNKALAVMVDHLESEKEGVIGCLDLILKWLTLRFFDTNTSV LMKALEYLKLLFTLLSEEEYHLTENEASSFIPYLVVKVGEPKDVIRKDVRAILNRMCLVY PASKMFPFIMEGTKSKNSKQRAECLEELGCLVESYGMNVCQPTPGKALKEIAVHIGDRDN AVRNAALNTIVTVYNVHGDQVFKLIGNLSEKDMSMLEERIKRSAKRPSAAPIKQVEEKPQ RAQNISSNANMLRKGPAEDMSSKLNQARSMSGHPEAAQMVRREFQLDLDEIENDNGTVRC EMPELVQHKLDDIFEPVLIPEPKIRAVSPHFDDMHSNTASTINFIISQVASGDINTSIQA LTQIDEVLRQEDKAEAMSGHIDQFLIATFMQLRLIYNTHMADEKLEKDEIIKLYSCIIGN MISLFQIESLAREASTGVLKDLMHGLITLMLDSRIEDLEEGQQVIRSVNLLVVKVLEKSD QTNILSALLVLLQDSLLATASSPKFSELVMKCLWRMVRLLPDTINSINLDRILLDIHIFM KVFPKEKLKQCKSEFPIRTLKTLLHTLCKLKGPKILDHLTMIDNKNESELEAHLCRMMKH SMDQTGSKSDKETEKGASRIDEKSSKAKVNDFLAEIFKKIGSKENTKEGLAELYEYKKKY SDADIEPFLKNSSQFFQSYVERGLRVIEMEREGKGRISTSTGISPQMEVTCVPTPTSTVS SIGNTNGEEVGPSVYLERLKILRQRCGLDNTKQDDRPPLTSLLSKPAVPTVASSTDMLHS KLSQLRESREQHQHSDLDSNQTHSSGTVTSSSSTANIDDLKKRLERIKSSRKL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
