Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860138
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4743nts
Open reading frame: 1 to 4743
ATGGAGGCGGCCACCACACTGCACCCAGGCCCGCGCCCGGCGCTGCCCCTCGGGGGCCCG
GGCCCGCTGGGCGAGTTCCTGCCTCCACCCGAGTGCCCGGTCTTCGAACCCAGCTGGGAA
GAGTTCGCGGACCCCTTCGCTTTCATCCACAAGATCCGGCCCATAGCCGAGCAGACTGGC
ATCTGTAAGGTGCGGCCGCCGCCGGATTGGCAGCCACCATTTGCATGTGATGTTGATAAA
CTTCATTTTACGCCACGTATCCAGAGACTGAATGAATTGGAGGCCCAAACTCGTGTAAAA
TTGAATTTCTTGGACCAGATTGCAAAGTACTGGGAGTTACAGGGAAGTACTCTGAAAATT
CCACATGTGGAGAGGAAGATCTTGGACTTATTTCAGCTTAATAAGTTAGTTGCAGAAGAA
GGTGGATTTGCAGTTGTTTGCAAGGATAGAAAATGGACCAAAATTGCTACCAAGATGGGG
TTTGCTCCTGGCAAAGCAGTGGGCTCACATATCAGAGGGCATTATGAACGAATTCTCAAC
CCCTACAACTTATTCCTGTCCGGAGACAGCCTAAGGTGTTTGCAGAAGCCAAACCTGACC
ACAGACACTAAGGACAAGGAGTACAAACCCCATGATATTCCCCAGAGGCAGTCTGTGCAG
CCTTCGGAAACGTGCCCCCCAGCCCGACGAGCAAAACGCATGAGAGCAGAGAGACAGAGT
CTCGCTGTGTTGCCCAGGCTGGAGTGCAGTGGCGCGATCTTGGCTCACTGCAACCTCCGC
CTCCTAGATTCCAGCAATTCTTCTGCCTCAGCCTCCCAAGCCATGAATATTAAAATAGAA
CCCGAGGAGACAACGGAAGCCAGAACTCATAATCTGAGACGTCGAATGGGTTGTCCAACT
CCAAAATGTGAAAATGAGAAAGAAATGAAGAGTAGCATCAAGCAAGAACCTATTGAGAGG
AAAGATTATATTGTAGAAAATGAGAAGGAAAAGCCCAAGAGTCGATCTAAAAAAGCCACC
AATGCTGTGGACCTGTATGTCTGTCTTTTATGTGGCAGTGGCAATGATGAAGACCGGCTA
CTGTTGTGTGATGGCTGTGATGACAGTTACCATACCTTTTGCTTGATCCCACCTCTCCAT
GATGTTCCCAAGGGAGACTGGAGGTGTCCTAAGTGTTTGGCTCAGGAATGTAGTAAGCCA
CAAGAAGCATTTGGCTTTGAACAAGCAGCCAGGGACTATACCCTCCGTACTTTTGGGGAA
ATGGCAGATGCGTTCAAATCTGATTACTTCAACATGCCAGTCCATATGGTCCCCACAGAG
CTTGTTGAGAAAGAATTTTGGAGACTAGTAAGCACTATTGAGGAGGATGTCACAGTGGAA
TATGGAGCTGACATTGCCTCAAAGGAATTTGGCAGTGGCTTTCCTGTCCGAGATGGGAAA
ATCAAACTCTCACCTGAGGAAGAGGAGTATCTTGATAGTGGCTGGAATTTGAACAACATG
CCAGTGATGGAGCAGTCTGTCCTTGCACATATTACTGCTGATATATGTGGCATGAAACTT
CCTTGGTTGTATGTGGGAATGTGCTTTTCTTCATTCTGTTGGCACATTGAAGACCACTGG
AGCTATTCAATTAACTACTTGCACTGGGGTGAGCCAAAAACCTGGTATGGAGTCCCAGGG
TATGCTGCTGAGCAGCTAGAAAATGTAATGAAGAAACTAGCTCCAGAACTCTTTGTGTCC
CAGCCGGATCTCCTCCATCAGCTTGTGACCATCATGAACCCCAATACCCTGATGACTCAT
GAAGTGCCTGTTTACCGAACTAATCAGTGTGCTGGGGAGTTTGTGATTACATTTCCAAGA
GCCTACCACAGTGGTTTTAACCAGGGTTTTAATTTTGCTGAGGCTGTTAACTTCTGCACT
GTTGATTGGCTGCCATTAGGCCGACAGTGTGTGGAGCATTATCGCTTGCTTCATCGATAT
TGTGTGTTTTCCCACGATGAGATGATCTGCAAGATGGCTTCCAAGGCTGATGTATTAGAT
GTTGTAGTGGCTTCAACTGTTCAGAAAGACATGGCCATTATGATTGAGGATGAGAAAGCT
TTAAGAGAAACTGTCCGTAAATTGGGAGTGATTGATTCGGAAAGAATGGATTTTGAGCTG
TTGCCAGATGATGAACGTCAGTGTGTAAAATGCAAAACTACATGCTTCATGTCTGCCATC
TCCTGTTCTTGTAAACCTGGCCTTCTTGTTTGCCTGCATCATGTAAAAGAATTGTGTTCC
TGTCCTCCTTACAAATATAAATTGCGGTATAGGTACACGCTGGATGATCTCTACCCTATG
ATGAATGCATTGAAGCTTCGAGCAGAATCTTACAACGAATGGGCCTTGAATGTGAATGAA
GCTTTGGAGGCAAAGATCAACAAGAAGAAAAGCCTTGTCAGCTTCAAGGCTTTAATTGAA
GAATCTGAAATGAAGAAATTCCCAGACAATGATCTTTTGCGACACCTTCGCCTAGTCACA
CAGGATGCAGAGAAGTGTGCCTCTGTTGCGCAGCAGTTGCTTAATGGCAAAAGGCAAACT
AGATATCGATCTGGTGGAGGGAAATCCCAAAATCAGTTGACAGTGAATGAGCTCCGGCAG
TTTGTAACACAGCTGTATGCTCTTCCATGTGTCCTCAGTCAGACACCATTACTAAAGGAT
CTCTTGAATCGTGTAGAAGATTTTCAACAGCATAGTCAGAAACTACTCTCTGAGGAAACG
CCTAGTGCTGCGGAGCTGCAGGACTTGCTAGATGTCAGCTTTGAATTTGATGTTGAACTT
CCACAGCTTGCTGAGATGCGTATCCGTTTGGAACAAGCCCGTTGGCTAGAAGAGGTGCAG
CAAGCTTGCCTAGACCCCAGCTCCCTTACTTTAGATGATATGAGACGTCTCATAGACCTA
GGGGTAGGGCTGGCCCCGTATTCAGCAGTGGAGAAAGCTATGGCCCGGCTGCAGGAACTG
CTCACAGTGTCAGAGCACTGGGACGACAAAGCCAAGAGTCTCCTCAAGGCCAGGCCACGA
CATTCATTGAATAGCCTTGCTACGGCAGTAAAGGAAATCGAAGAGATCCCTGCATATCTG
CCCAATGGTGCGGCTCTGAAAGACTCAGTGCAGAGAGCCAGAGACTGGCTTCAGGATGTA
GAGGGCCTGCAGGCTGGAGGACGTGTGCCAGTGTTAGACACACTCATAGAACTTGTTACA
CGAGGCCGATCTATCCCCGTACATCTGAATTCTTTGCCAAGACTGGAAACCCTAGTAGCT
GAGGTTCAGGCTTGGAAAGAATGTGCTGTTAATACATTCTTGACTGAGAATTCTCCATAT
TCTCTCTTAGAGGTGCTGTGTCCTCGATGTGATATTGGCCTTTTGGGATTGAAAAGGAAG
CAGAGAAAGTTAAAGGAGCCCTTGCCAAATGGAAAGAAAAAAAGCACCAAATTAGAGAGT
CTGAGTGACCTGGAGAGAGCTTTAACTGAAAGCAAGGAGACTGCTTCAGCTATGGCAACT
CTTGGGGAAGCTCGCCTAAGGGAAATGGAAGCCTTGCAGTCTCTCAGACTCGCCAATGAA
GGGAAATTGCTGTCGCCTCTCCAAGATGTGGATATAAAAATCTGCCTATGTCAGAAGGCC
CCAGCTGCCCCTATGATTCAATGTGAACTCTGCAGGGATGCTTTCCACACCAGTTGTGTG
GCGGTACCCAGTATTTCACAGGGCCTGCGAATCTGGCTTTGTCCCCATTGTCGGAGGTCA
GAGAAACCTCCATTAGAGAAAATTCTGCCCCTGCTCGCCTCCCTTCAGCGTATCCGAGTT
CGCCTTCCTGAGGGAGATGCACTTCGATATATGATTGAAAGAACCGTGAACTGGCAGCAC
AGAGCCCAGCAACTGCTTTCGTCAGGGAATCTTAAATTTGTGCAAGATCGAGTGGGCTCA
GGACTGTTATATAGCAGATGGCAAGCCTCAGCAGGACAGGTGTCAGACACAAACAAGGTA
TCTCAACCTCCTGGCACAACATCATTTTCTTTGCCTGATGACTGGGACAACAGAACCTCA
TATTTGCACTCCCCCTTCTCAACTGGACGAAGTTGTATCCCCCTCCATGGTGTTAGTCCA
GAAGTGAATGAACTATTGATGGAAGCCCAGCTGCTCCAGGTATCCCTTCCTGAAATTCAG
GAACTTTACCAGACTTTACTTGCAAAGCCAAGCCCTGCTCAGCAGACTGACCGAAGCTCA
CCAGTGAGACCCAGCAGTGAGAAGAATGACTGTTGCCGAGGGAAGCGAGATGGAATTAAC
AGTCTTGAGAGAAAACTGAAGAGACGCCTGGAAAGAGAGGGCCTCTCCAGTGAGCGGTGG
GAACGAGTTAAGAAAATGCGGACCCCCAAAAAGAAGAAAATCAAACTGAGCCACCCCAAG
GACATGAACAATTTCAAGTTAGAGAGAGAGCGTAGCTATGAATTAGTTCGTTCTGCTGAA
ACTCATTCCCTGCCCTCAGACACATCCTATTCCGAACAGGAAGACTCTGAGGATGAAGAT
GCCATCTGCCCAGCTGTGAGCTGCCTGCAGCCAGAAGGAGATGAGGTGGACTGGGTCCAG
TGTGATGGCAGCTGCAATCAGTGGTTTCATCAGGTCTGTGTTGGTGTCTCCCCAGAGATG
GCAGAGAAAGAAGACTACATCTGTGTGCGCTGTACTGTGAAGGACGCACCAAGCCGAAAG
TTG
Protein sequence predicted based on nucleotide sequence
MEAATTLHPGPRPALPLGGPGPLGEFLPPPECPVFEPSWEEFADPFAFIHKIRPIAEQTG ICKVRPPPDWQPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKYWELQGSTLKI PHVERKILDLFQLNKLVAEEGGFAVVCKDRKWTKIATKMGFAPGKAVGSHIRGHYERILN PYNLFLSGDSLRCLQKPNLTTDTKDKEYKPHDIPQRQSVQPSETCPPARRAKRMRAERQS LAVLPRLECSGAILAHCNLRLLDSSNSSASASQAMNIKIEPEETTEARTHNLRRRMGCPT PKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGNDEDRL LLCDGCDDSYHTFCLIPPLHDVPKGDWRCPKCLAQECSKPQEAFGFEQAARDYTLRTFGE MADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGK IKLSPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHW SYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTH EVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRY CVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFEL LPDDERQCVKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYKLRYRYTLDDLYPM MNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVT QDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKD LLNRVEDFQQHSQKLLSEETPSAAELQDLLDVSFEFDVELPQLAEMRIRLEQARWLEEVQ QACLDPSSLTLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLKARPR HSLNSLATAVKEIEEIPAYLPNGAALKDSVQRARDWLQDVEGLQAGGRVPVLDTLIELVT RGRSIPVHLNSLPRLETLVAEVQAWKECAVNTFLTENSPYSLLEVLCPRCDIGLLGLKRK QRKLKEPLPNGKKKSTKLESLSDLERALTESKETASAMATLGEARLREMEALQSLRLANE GKLLSPLQDVDIKICLCQKAPAAPMIQCELCRDAFHTSCVAVPSISQGLRIWLCPHCRRS EKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKFVQDRVGS GLLYSRWQASAGQVSDTNKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGRSCIPLHGVSP EVNELLMEAQLLQVSLPEIQELYQTLLAKPSPAQQTDRSSPVRPSSEKNDCCRGKRDGIN SLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAE THSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEM AEKEDYICVRCTVKDAPSRKL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
