Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860148
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5256nts
Open reading frame: 1 to 5256
ATGGCTCCCTACCCCTGTGGCTGCCACATCCTGCTGCTGCTCTTCTGCTGCCTGGCGGCT
GCCCGGGCCAACCTGCTGAACCTGAACTGGCTTTGGTTCAATAATGAGGACACCAGCCAT
GCAGCTACCACGATCCCTGAGCCCCAGGGGCCCCTGCCTGTGCAGCCCACAGCAGATACC
ACCACACACGTGACCCCCCGGAATGGTTCCACAGAGCCAGCGACAGCCCCTGGCAGCCCT
GAGCCACCCTCAGAGCTGCTGGAAGATGGCCAGGACACCCCCACTTCTGCCGAGAGCCCG
GACGCGCCAGAGGAGAACATTGCCGGTGTCGGAGCCGAGATCCTGAACGTGGCCAAAGGC
ATCCGGAGCTTCGTCCAGCTGTGGAATGACACTGTCCCCACTGAGAGCTTGGCCAGGGCG
GAAACCCTGGTCCTGGAGACTCCTGTGGGCCCCCTTGCCCTCGCTGGGCCTTCCAGCACC
CCCCAGGAGAATGGGACCACTCTCTGGCCCAGCCGTGGCATTCCTAGCTCTCCGGGCGCC
CACACAACCGAGGCTGGCACCTTGCCTGCACCCACCCCATCGCCTCCCTCCCTGGGCAGG
CCCTGGGCACCACTCACGGGGCCCTCAGTGCCACCACCATCTTCAGGTAGAGCTTCTCTC
TCCTCCTTGCTGGGCGGGGCCCCTCCCTGGGGAAGCCTGCAGGACCCAGACAGCCAAGGA
CTCTCGCCCGCCGCAGCCGCTCCCAGCCAGCAGCTCCAACGCCCTGACGTCCGCCTGCGC
ACGCCACTTCTGCACCCCCTGGTGATGGGCTCCCTGGGCAAGCACGCGGCCCCCTCCGCC
TTCTCCTCTGGGCTCCCGGGCGCACTGTCTCAGGTCGCAGTCACCACTTTAACCAGGGAC
AGCGGTGCTTGGGTCTCCCACGTGGCTAACTCTGTGGGGCCGGGTCTTGCTAATAACTCT
GCCCTGCTCGGGGCTGACCCCGAGGCCCCCGCCGGTCGCTGCCTGCCCCTGCCACCCTCC
CTGCCAGTCTGCGGCCACCTGGGCATCTCACGCTTCTGGCTGCCCAACCACCTCCACCAC
GAGAGCGGCGAGCAGGTGCGGGCCGGGGCACGGGCGTGGGGGGGCCTGCTGCAGACGCAC
TGCCACCCCTTCCTCGCCTGGTTCTTCTGCCTGCTGCTGGTCCCCCCATGCGGCAGCGTC
CCGCCGCCCGCCCCGCCACCCTGCTGCCAGTTCTGCGAGGCCCTGCAGGATGCGTGTTGG
AGCCGCCTGGGCGGGGGCCGGCTGCCCGTCGCCTGTGCCTCGCTCCCGACCCAGGAGGAT
GGGTACTGTGTGCTCATTGGGCCGGCTGCAGAGCGCATCAGCGAGGAGGTGGGGCTGCTG
CAGCTCCTTGGGGACCCCCCGCCCCAGCAGGTCACCCAGACGGATGACCCCGACGTCGGG
CTGGCCTACGTCTTTGGGCCAGATGCCAACAGTGGCCAAGTGGCCCGGTACCACTTCCCC
AGCCTCTTCTTCCGTGACTTCTCACTGCTGTTCCACATCCGGCCAGCCACAGAGGGCCCA
GGGGTGCTGTTCGCCATCACGGACTCGGCGCAGGCCATGGTCTTGCTGGGCGTGAAGCTC
TCTGGGGTGCAGGACGGGCACCAGGACATCTCCCTGCTCTACACAGAACCAGGTGCAGGC
CAGACCCACACAGCCGCCAGCTTCCGGCTCCCCGCCTTCGTCGGCCAGTGGACACACTTA
GCCCTCAGTGTGGCAGGTGGCTTTGTGGCCCTCTACGTGGACTGTGAGGAGTTCCAGAGA
ATGCCGCTTGCTCGGTCCTCACGGGGCCTGGAGCTGGAGCCTGGCGCCGGGCTCTTCGTG
GCTCAGGCGGGGGGAGCGGACCCTGACAAGTTCCAGGGGGTGATCGCTGAGCTGAAGGTG
CGCAGGGACCCCCAGGTGAGCCCCATGCACTGCCTGGACGAGGAAGGCGATGACTCAGAT
GGGGCATCCGGAGACTCTGGCAGCGGGCTCGGGGACGCCCGGGAGCTTCTCAGGGAGGAG
ACGGGCGCGGCCCTAAAACCCAGGCTCCCCGCGCCACCCCCCGTCACCACGCCACCCTTG
GCTGGAGGCAGCAGCACGGAAGATTCCAGAAGTGAAGAAGTCGAGGAGCAGACCACGGTG
GCTTCGTTAGGAGCTCAGACACTTCCTGGCTCAGATTCTGTCTCCACGTGGGACGGGAGT
GTCCGGACCCCTGGGGGCCGCGTGAAAGAGGGCGGCCTGAAGGGGCAGAAAGGGGAGCCA
GGTGTTCCGGGCCCACCTGGCCGGGCAGGCCCCCCAGGATCCCCATGCCTACCTGGTCCC
CCGGGTCTCCCGTGCCCAGTGAGTCCCCTGGGTCCTGCAGGCCCAGCGTTGCAAACTGTC
CCCGGACCACAAGGACCCCCAGGGCCTCCGGGGAGGGACGGCACCCCTGGAAGGGACGGC
GAGCCGGGCGACCCCGGTGAAGACGGAAAGCCGGGCGACACCGGGCCACAAGGCTTCCCC
GGGACTCCAGGGGACGTAGGTCCCAAGGGCGACAAGGGAGACCCTGGGGTTGGAGAGAGA
GGGCCCCCAGGACCCCAAGGGCCTCCAGGGCCCCCAGGACCCTCCTTCAGACACGACAAG
CTGACCTTCATTGACATGGAGGGATCTGGCTTCGGGGGCGATCTGGAGGCCCTGCGGGGT
CCTCGAGGCTTCCCTGGACCTCCCGGACCCCCCGGTGTCCCAGGCCTGCCCGGCGAGCCA
GGCCGCTTTGGGGTGAACAGCTCCGACGTCCCAGGACCCGCCGGCCTTCCTGGTGTGCCT
GGGCGCGAGGGTCCCCCCGGGTTTCCTGGCCTCCCGGGACCCCCAGGCCCTCCGGGAAGA
GAGGGGCCCCCAGGAAGGACTGGGCAGAAAGGCAGCCTGGGTGAAGCAGGCGCCCCAGGA
CATAAGGGGAGCAAGGGAGCCCCCGGTCCTGCTGGTGCTCGTGGGGAGAGCGGCCTGGCA
GGAGCCCCCGGACCTGCTGGACCACCAGGCCCCCCTGGGCCCCCTGGGCCCCCAGGACCA
GGACTCCCCGCTGGATTTGATGACATGGAAGGCTCCGGGGGGCCCTTCTGGTCAACAGCC
CGAAGCGCTGATGGGCCACAGGGACCTCCCGGCCTGCCGGGACTTAAGGGGGATCCTGGC
GTGCCTGGGCTGCCGGGGGCGAAGGGAGAAGTTGGAGCAGATGGAGTCCCCGGGTTCCCC
GGCCTCCCTGGCAGAGAGGGCATTGCTGGGCCCCAGGGGCCAAAGGGAGACAGAGGCAGC
CGGGGAGAAAAGGGAGATCCAGGGAAGGACGGAGTCGGGCAGCCGGGCCTCCCTGGCCCC
CCCGGACCCCCGGGACCTGTGGTCTACGTGTCGGAGCAGGACGGATCCGTCCTGAGCGTG
CCGGGACCTGAGGGCCGGCCGGGTTTCGCAGGCTTTCCCGGACCTGCAGGACCCAAGGGC
AACCTGGGCTCTAAGGGCGAACGAGGCTCCCCGGGACCCAAGGGTGAGAAGGGTGAACCG
GGCAGCATCTTCAGCCCCGACGGCGGTGCCCTGGGCCCTGCCCAGAAAGGAGCCAAGGGA
GAGCCGGGCTTCCGAGGACCCCCGGGTCCATACGGACGGCCGGGGTACAAGGGAGAGATT
GGCTTTCCTGGACGGCCGGGTCGCCCCGGGATGAACGGATTGAAAGGAGAGAAAGGGGAG
CCGGGAGATGCCAGCCTTGGATTTGGCATGAGGGGAATGCCCGGCCCCCCAGGACCTCCA
GGGCCCCCAGGCCCTCCAGGGACTCCTGTTTACGACAGCAATGTGTTTGCTGAGTCCAGC
CGCCCCGGGCCTCCAGGATTGCCAGGGAATCAGGGCCCTCCAGGACCCAAGGGCGCCAAA
GGAGAAGTGGGCCCCCCCGGACCACCAGGGCAGTTTCCGTTTGACTTTCTTCAGTTGGAG
GCTGAAATGAAGGGGGAGAAGGGAGACCGAGGTGATGCAGGACAGAAAGGCGAAAGGGGG
GAGCCCGGGGGCGGCGGTTTCTTCGGCTCCAGCCTGCCCGGCCCCCCCGGCCCCCCAGGC
CCACGTGGCTACCCTGGGATTCCAGGTCCCAAGGGAGAGAGCATCCGGGGCCAGCCCGGC
CCACCTGGACCTCAGGGACCCCCCGGCATCGGCTACGAGGGGCGCCAGGGCCCTCCCGGC
CCCCCAGGCCCCCCAGGGCCCCCTTCATTTCCTGGCCCTCACAGGCAGACTATCAGCGTT
CCCGGCCCTCCGGGCCCCCCTGGGCCCCCTGGGCCCCCTGGAACCATGGGCGCCTCCTCA
GGGGTGAGGCTCTGGGCTACACGCCAGGCCATGCTGGGCCAGGTGCACGAGGTTCCCGAG
GGCTGGCTCATCTTCGTGGCCGAGCAGGAGGAGCTCTACGTCCGCGTGCAGAACGGGTTC
CGGAAGGTCCAGCTGGAGGCCCGGACACCACTCCCACGAGGGACGGACAATGAAGTGGCC
GCCTTGCAGCCCCCCGTGGTGCAGCTGCACGACAGCAACCCCTACCCGCGGCGGGAGCAC
CCCCACCCCACCGCGCGGCCCTGGCGGGCAGATGACATCCTGGCCAGCCCCCCTCGCCTG
CCCGAGCCCCAGCCCTACCCCGGAGCCCCGCACCACAGCTCCTACGTGCACCTGCGGCCG
GCGCGACCCACAAGCCCACCCGCCCACAGCCACCGCGACTTCCAGCCGGTGCTCCACCTG
GTTGCGCTCAACAGCCCCCTGTCAGGCGGCATGCGGGGCATCCGCGGGGCCGACTTCCAG
TGCTTCCAGCAGGCGCGGGCCGTGGGGCTGGCGGGCACCTTCCGCGCCTTCCTGTCCTCG
CGCCTGCAGGACCTGTACAGCATCGTGCGCCGTGCCGACCGCGCAGCCGTGCCCATCGTC
AACCTCAAGGACGAGCTGCTGTTTCCCAGCTGGGAGGCTCTGTTCTCAGGCTCTGAGGGT
CCGCTGAAGCCCGGGGCACGCATCTTCTCCTTTGACGGCAAGGACGTCCTGAGGCACCCC
ACCTGGCCCCAGAAGAGCGTGTGGCATGGCTCGGACCCCAACGGGCGCAGGCTGACCGAG
AGCTACTGTGAGACGTGGCGGACGGAGGCTCCCTCGGCCACGGGCCAGGCCTCCTCGCTG
CTGGGGGGCAGGCTCCTGGGGCAGAGTGCCGCGAGCTGCCATCACGCCTACATCGTGCTC
TGCATTGAGAACAGCTTCATGACTGCCTCCAAGTTG
Protein sequence predicted based on nucleotide sequence
MAPYPCGCHILLLLFCCLAAARANLLNLNWLWFNNEDTSHAATTIPEPQGPLPVQPTADT TTHVTPRNGSTEPATAPGSPEPPSELLEDGQDTPTSAESPDAPEENIAGVGAEILNVAKG IRSFVQLWNDTVPTESLARAETLVLETPVGPLALAGPSSTPQENGTTLWPSRGIPSSPGA HTTEAGTLPAPTPSPPSLGRPWAPLTGPSVPPPSSGRASLSSLLGGAPPWGSLQDPDSQG LSPAAAAPSQQLQRPDVRLRTPLLHPLVMGSLGKHAAPSAFSSGLPGALSQVAVTTLTRD SGAWVSHVANSVGPGLANNSALLGADPEAPAGRCLPLPPSLPVCGHLGISRFWLPNHLHH ESGEQVRAGARAWGGLLQTHCHPFLAWFFCLLLVPPCGSVPPPAPPPCCQFCEALQDACW SRLGGGRLPVACASLPTQEDGYCVLIGPAAERISEEVGLLQLLGDPPPQQVTQTDDPDVG LAYVFGPDANSGQVARYHFPSLFFRDFSLLFHIRPATEGPGVLFAITDSAQAMVLLGVKL SGVQDGHQDISLLYTEPGAGQTHTAASFRLPAFVGQWTHLALSVAGGFVALYVDCEEFQR MPLARSSRGLELEPGAGLFVAQAGGADPDKFQGVIAELKVRRDPQVSPMHCLDEEGDDSD GASGDSGSGLGDARELLREETGAALKPRLPAPPPVTTPPLAGGSSTEDSRSEEVEEQTTV ASLGAQTLPGSDSVSTWDGSVRTPGGRVKEGGLKGQKGEPGVPGPPGRAGPPGSPCLPGP PGLPCPVSPLGPAGPALQTVPGPQGPPGPPGRDGTPGRDGEPGDPGEDGKPGDTGPQGFP GTPGDVGPKGDKGDPGVGERGPPGPQGPPGPPGPSFRHDKLTFIDMEGSGFGGDLEALRG PRGFPGPPGPPGVPGLPGEPGRFGVNSSDVPGPAGLPGVPGREGPPGFPGLPGPPGPPGR EGPPGRTGQKGSLGEAGAPGHKGSKGAPGPAGARGESGLAGAPGPAGPPGPPGPPGPPGP GLPAGFDDMEGSGGPFWSTARSADGPQGPPGLPGLKGDPGVPGLPGAKGEVGADGVPGFP GLPGREGIAGPQGPKGDRGSRGEKGDPGKDGVGQPGLPGPPGPPGPVVYVSEQDGSVLSV PGPEGRPGFAGFPGPAGPKGNLGSKGERGSPGPKGEKGEPGSIFSPDGGALGPAQKGAKG EPGFRGPPGPYGRPGYKGEIGFPGRPGRPGMNGLKGEKGEPGDASLGFGMRGMPGPPGPP GPPGPPGTPVYDSNVFAESSRPGPPGLPGNQGPPGPKGAKGEVGPPGPPGQFPFDFLQLE AEMKGEKGDRGDAGQKGERGEPGGGGFFGSSLPGPPGPPGPRGYPGIPGPKGESIRGQPG PPGPQGPPGIGYEGRQGPPGPPGPPGPPSFPGPHRQTISVPGPPGPPGPPGPPGTMGASS GVRLWATRQAMLGQVHEVPEGWLIFVAEQEELYVRVQNGFRKVQLEARTPLPRGTDNEVA ALQPPVVQLHDSNPYPRREHPHPTARPWRADDILASPPRLPEPQPYPGAPHHSSYVHLRP ARPTSPPAHSHRDFQPVLHLVALNSPLSGGMRGIRGADFQCFQQARAVGLAGTFRAFLSS RLQDLYSIVRRADRAAVPIVNLKDELLFPSWEALFSGSEGPLKPGARIFSFDGKDVLRHP TWPQKSVWHGSDPNGRRLTESYCETWRTEAPSATGQASSLLGGRLLGQSAASCHHAYIVL CIENSFMTASKL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
