Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860195
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5580nts
Open reading frame: 1 to 5580
ATGGTGGATAAGAATATTTACATCATTCAAGGGGAGATTAACATTGTGGTTGGGGCCATC
AAACGAAATGCCCGATGGAGCACCCATACACCACTGGATGAAGAACGGGATCCTCTGCTG
CATAGTTTCGGTCATCTAAAGGAGGTTTTAAACAGTATAACAGAACTCTCAGAAATTGAG
CCCAATGTATTCCTTCGACCTTTTCTGGAAGTGATTCGCTCTGAAGATACCACTGGCCCT
ATCACTGGACTGGCACTCACCTCTGTCAACAAGTTCCTGTCCTATGCACTCATAGATCCC
ACCCATGAGGGCACAGCAGAGGGCATGGAGAACATGGCAGATGCTGTCACCCATGCTCGT
TTTGTGGGCACGGATCCTGCCAGTGATGAAGTTGTCCTGATGAAAATCCTTCAGGTTCTA
CGGACTCTGCTGCTAACCCCAGTGGGTGCCCACCTAACCAATGAATCTGTGTGTGAGATT
ATGCAGTCTTGCTTCCGGATCTGCTTTGAAATGAGGCTCAGTGAGTTATTGAGAAAATCC
GCAGAGCACACTCTCGTAGACATGGTGCAGCTGCTCTTCACAAGGTTACCTCAGTTTAAA
GAAGAACCCAAGAACTATGTGGGGACCAACATGAAGAAGCTGAAAATGAGAGCCGGAGGC
ATGAGTGATTCATCCAAATGGAAGAAACAGAAGAGATCCCCTCGGCCCCCACGCCATATG
ACCAAAGTCACACCAGGTTCAGAGCTGCCCACTCCCAATGGAACCACCTTATCATCTAAC
CTCACTGGTGGCATGCCCTTCATTGATGTGCCCACTCCCATCTCCTCTGCAAGTTCAGAA
GCTGCCTCAGCAGTGGTCAGTCCCTCTACAGACAGTGGCCTGGAATTCTCCTCCCAAACC
ACTTCCAAGGAAGACCTTACTGATCTAGAGCAACCTGGCTCTCCAGGGTACAGCACAGCT
ACAGAGCCTGGAAGCAGTGAGCTAGGTGTTCCCGAGCAGCCTGACCTCCAGGAAGGGACC
CATGTGGAAAAGTCCCAGTCAGCATCTGTGGAGTCCATCCCTGAAGTGTTAGAGGAGTGC
ACGTCCCCTGCCGACCACTCTGACTCTGCCTCTGTCCATGACATGGATTACGTCAATCCC
CGGGGCGTGCGCTTTACACAGTCCTCCCAGAAAGAAGGCACAGCTTTGGTCCCCTATGGT
CTTCCCTGCATCCGCGAGCTCTTCCGCTTCCTCATCTCCCTCACCAATCCACACGACCGC
CATAACTCAGAGGTTATGATTCACATGGGACTGCATTTGCTGACAGTGGCCCTTGAGTCA
GCCCCTGTAGCCCAGTGCCAGACCCTCTTGGGCCTCATCAAGGATGAGATGTGCCGTCAC
TTATTCCAGCTACTCAGCATAGAGCGACTAAACCTTTATGCTGCTTCCCTGCGAGTATGC
TTCCTACTGTTTGAGAGCATGCGAGAGCACCTCAAGTTCCAAATGGAGATGTACATCAAA
AAGCTTATGGAGATCATCACTGTGGAGAACCCCAAGATGCCTTATGAGATGAAGGAGATG
GCACTGGAGGCCATTGTGCAGCTCTGGCGCATCCCCAGCTTTGTCACAGAGCTCTACATC
AACTATGATTGTGACTACTACTGTTCCAACCTCTTTGAGGAACTCACAAAGCTGCTGTCC
AAGAATGCCTTCCCTGTGTCTGGTCAACTCTATACAACACACCTACTATCTCTTGATGCC
CTATTGACAGTGATTGACAGCACCGAGGCCCACTGCCAGGCTAAAGTCCTCAACAGCCTC
ACCCAGCAAGAGAAGAAGGAGACAGCCAGACCAAGCTGTGAGATAGTAGATGGCACCCGA
GAAGCTAGCAATACTGAGAGAACTGCCAGCGATGGGAAAGCTGTAGGCATGGCCTCAGAC
ATCCCAGGCCTGCATCTGCCAGGTGGAGGGCGGCTGCCACCAGAACATGGGAAATCAGGA
TGCAGTGATCTGGAGGAAGCTGTTGACTCTGGGGCTGACAAAAAGTTTGCCCGGAAGCCA
CCCCGATTTTCCTGTCTCCTGCCAGATCCACGGGAACTAATTGAAATTAAAAACAAAAAG
AAGCTGCTAATCACTGGCACAGAGCAGTTCAATCAGAAACCAAAGAAGGGGATTCAGTTT
CTGCAAGAGAAAGGCCTCCTCACCATCCCAATGGACAACACAGAGGTTGCTCAGTGGCTC
CGAGAGAACCCTCGGCTGGACAAGAAGATGATTGGAGAGTTTGTGAGTGACCGCAAAAAC
ATTGACCTGTTGGAGAGCTTTGTGAGCACCTTCAGTTTTCAGGGTCTGCGACTGGACGAA
GCCCTCCGCCTCTACCTGGAAGCCTTCCGTTTGCCTGGGGAAGCACCAGTCATCCAGAGG
TTGCTGGAGGCATTCACAGAGCGTTGGATGAATTGTAATGGCTCCCCATTTGCCAATAGC
GATGCCTGCTTTTCCCTGGCCTATGCTGTCATCATGCTTAATACTGACCAGCACAACCAC
AATGTTCGTAAACAGAATGCACCCATGACCCTGGAGGAGTTTCGCAAAAATCTGAAAGGT
GTGAATGGAGGCAAGGACTTTGAGCAAGACATCCTGGAGGACATGTACCATGCCATCAAG
AATGAGGAAATTGTAATGCCTGAGGAGCAGACAGGCTTGGTTCGGGAGAACTATGTGTGG
AATGTGCTGCTTCATCGAGGTGCCACCCCTGAGGGCATATTCCTGCGTGTGCCTACTGCC
AGCTATGATCTTGACCTCTTCACCATGACCTGGGGCCCCACTATTGCTGCTCTCTCTTAT
GTCTTTGACAAAAGCCTTGAGGAGACAATCATCCAGAAAGCCATCTCAGGCTTCAGGAAG
TGCGCCATGATCTCCGCCCACTATGGCCTCAGCGATGTGTTTGACAATCTCATCATCTCT
CTATGCAAATTCACAGCTCTCAGCAGTGAGTCTATTGAGAACCTGCCCAGTGTATTTGGA
AGCAACCCTAAAGCCCATATTGCAGCCAAGACAGTATTCCATTTGGCCCATCGTCATGGT
GACATCCTGCGGGAGGGCTGGAAGAATATCATGGAGGCCATGCTGCAGCTCTTCCGAGCC
CAACTACTGCCCAAGGCTATGATAGAGGTAGAAGATTTCGTGGATCCCAATGGCAAGATC
TCTCTACAGCGGGAAGAGACACCATCAAACCGAGGAGAGTCAACAGTGCTGAGCTTTGTG
AGCTGGCTAACACTGAGTGGTCCTGAGCAGTCTAGTGTTCGGGGCCCATCCACTGAAAAC
CAAGAGGCCAAGAGAGTGGCCTTAGAGTGTATAAAGCAATGTGACCCAGAAAAAATGATC
ACAGAAAGCAAGTTCCTCCAGCTGGAGTCACTACAGGAGCTCATGAAGGCTCTGGTCTCA
GTGACACCAGATGAAGAGACATATGATGAGGAAGATGCTGCTTTCTGCCTAGAGATGCTG
CTAAGGATTGTGTTGGAGAACAGGGATCGTGTGGGCTGTGTGTGGCAGACTGTTCGAGAC
CATCTATACCACCTCTGTGTTCAGGCACAAGATTTCTGCTTCCTTGTGGAGCGGGCAGTG
GTGGGGTTGCTACGCCTGGCCATTCGGCTTCTCCGGAGAGAAGAGATCAGTGCTCAGGTG
CTGCTCTCCCTGCGCATTTTGCTACTGATGAAGCCCAGTGTGCTATCCCGAGTCAGCCAC
CAGGTTGCGTATGGGCTCCATGAACTCCTGAAGACCAATGCAGCCAACATCCACTCAGGT
GATGACTGGGCCACACTCTTCACACTGCTGGAGTGCATCGGCTCAGGTGTGAAGCCTCCA
GCTGCTCTGCAGGCCACAGCCAGGGCAGATGCACCTGATGCCGGGGCCCAGTCAGATAGT
GAGCTCCCATCCTACCATCAGAATGACGTGAGCCTGGATCGAGGGTACACTTCCGACTCA
GAGGTCTACACTGACCATGGCAGGCCGGGCAAGATACACCGATCAGCCACAGATGCCGAT
GTGGTCAACAGTGGTTGGTTAGTGGTTGGGAAGGATGACGTTGATAACTCCAAGCCAGGG
CCCAGCCGCCCAGGCCCTTCACCCCTGATCAATCAATACAGCCTAACAGTGGGACTGGAT
TTGGGGCCACACGACACTAAGTCTCTGCTTAAGTGTGTGGAATCGCTGTCCTTCATCGTG
CGTGATGCTGCCCACATCACACCTGACAACTTTGAGCTCTGCGTCAAGACTCTCCGGATC
TTTGTGGAGGCCAGTCTGAATGGCGGATGCAAGTCCCAGGAGAAACGTGGCAAGAGTCAC
AAATATGACAGCAAAGGGAACCGCTTCAAGAAGAAATCCAAAGAGGGATCAATGCTTCGC
CGGCCTCGAACCTCCAGCCAACATGCCTCTCGGGGCGGGCAGAGTGATGATGATGAGGAC
GAAGGCGTGCCTGCCAGCTACCATACGGTGTCTTTACAGGTCAGTCAGGACTTGCTAGAC
CTGATGCACACCCTGCACACGCGGGCAGCCTCTATCTACAGCTCATGGGCGGAGGAGCAA
CGCCACCTGGAGACAGGTGGCCAGAAGATTGAAGCTGATTCTCGCACCCTCTGGGCCCAC
TGCTGGTGCCCTTTACTGCAGGGTATTGCCTGCCTGTGCTGCGATGCCCGGCGCCAGGTA
CGGATGCAGGCACTGACCTATCTGCAGCGAGCACTACTTGTACATGATCTGCAAAAGCTA
GATGCCCTGGAATGGGAGTCCTGTTTTAACAAGGTGCTGTTTCCTCTACTTACCAAGCTC
TTGGAGAACATCAGCCCTGCAGATGTGGGTGGGATGGAGGAGACCCGGATGAGGGCTTCC
ACATTGCTCTCTAAGGTCTTCCTGCAGCACCTGTCTCCACTGCTGTCACTCTCTACCTTT
GCGGCCCTCTGGCTCACCATCTTGGACTTCATGGACAAGTACATGCACGCAGGCTCCAGC
GACTTACTGTCAGAGGCGATCCCTGAGTCTCTGAAGAACATGCTTCTGGTGATGGACACA
GCGGAGATTTTCCACAGTGCAGATGCACGGGGAGGCGGCCCCTCGGCCCTCTGGGAGATC
ACCTGGGAACGCATTGACTGTTTTCTCCCTCACCTACGAGATGAACTCTTCAAGCAGACC
GTCATCCAGGACCCCATGCCCATGGAGCCTCAAGGCCAAAAGCCTCTCGCCTCAGCCCAC
CTGACTTCCGCTGCTGGCGACACTAGGACACCTGGCCATCCACCGCCCCCAGAGATTCCA
TCTGAGCTGGGGGCCTGTGACTTTGAGAAGCCCGAGAGCCCCCGAGCCGCCAGCAGCAGC
TCCCCAGGATCACCAGTGGCCTCAAGCCCCAGCAGGCTGAGCCCCACCCCCGACGGGCCT
CCACCCTTGGCTCAGCCCCCACTGATCCTGCAGCCCTTGGCCTCCCCACTGCAGGTGGGC
GTGCCACCTATGACTCTGCCCATCATCCTCAACCCTGCGCTCATCGAGGCCACCTCACCA
GTGCCCCTCCTGGCCACACCCCGCCCCACAGATCCCATACCCACCTCTGAGGTCAACTTG
Protein sequence predicted based on nucleotide sequence
MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIE PNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHAR FVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKS AEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHM TKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQT TSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVLEEC TSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDR HNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVC FLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYI NYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSL TQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSG CSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQF LQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDE ALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNH NVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVW NVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRK CAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHG DILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFV SWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVS VTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAV VGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSG DDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDS EVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLD LGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSH KYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLD LMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQV RMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRAS TLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDT AEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAH LTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGP PPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVNL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
