Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860223
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4689nts
Open reading frame: 1 to 4689
ATGAGTGTAAGGTTACCCCAGAGTATAGACAGGTTAAGTAGCCTGTCTTCTCTGGGAGAT
TCTGCACCAGAGCGCAAGTCCCCTTCCCACCATCGCCAGCCTTCGGATGCCTCTGAGACA
ACAGGTCTCGTTCAACGCTGTGTCATTATCCAAAAGGACCAGCATGGCTTCGGCTTCACA
GTCAGTGGGGATCGCATTGTTCTGGTGCAGTCTGTGCGGCCTGGAGGTGCAGCCATGAAG
GCCGGTGTGAAAGAGGGCGACCGGATCATCAAAGTCAACGGCACCATGGTGACCAATAGC
TCACACCTGGAAGTGGTAAAGCTGATCAAATCTGGCGCCTATGTCGCACTCACCCTCCTG
GGCTCTTCACCTTCATCCATGGGCATCTCTGGGCTCCAGCAGGACCCATCCCCAGCAGGA
GCTCCCCGAATCACGTCAGTGATCCCCTCACCACCACCTCCTCCACCTCTACCACCTCCA
CAACGCATCACAGGACCCAAACCTCTGCAGGATCCCGAAGTTCAAAAACATGCCACCCAG
ATCCTCAGGAATATGCTGAGGCAGGAAGAAAAAGAATTACAGCGTATCTGTGAGGTCTAT
AGCCGGAACCCCGCCAGCCTACTGGAAGAGCAGATCGAAGGTGCCCGGCGGCGAGTCACT
CAGTTACAGCTGAAGATCCAGCAGGAGACTGGTGGCTCAGTGGACATACTTCCACTATAT
GGTGACACCAGCCAGAGACCATCAGAAGGCCGGCTCTCTCTGGATTCCCAGGAGGGGGAC
AGTGGCTTGGACTCTGGGACAGAACGCTTTCCTTCCCTCAGTGAGTCATTGATGAATCGG
AACTCGGTACTGTCAGACCCTGGGCTAGACAGTCCTCGAACCTCCCCTGTGATCATGGCC
AGGGTGGCCCAGCACCACAGGCGGCAGGGCTCGGATGCAGCAGTCCCCTCAACCGGTGAC
CAGGGTGTAGATCAAAGCCCAAAGCCTTTAATTATTGGCCCAGAGGAAGACTATGACCCG
GGTTATTTCAACAACGAGAGCGACATCATATTCCAGGATCTGGAGAAACTGAAGTCTCGG
CCAGCTCACCTGGGGGTTTTTCTACGTTACATCTTCTCTCAGGCGGACCCCAGTCCACTG
CTTTTTTACCTGTGTGCAGAAGTTTATCAGCAGGCAAGCCCCAAGGATTCCCGAAGCTTG
GGGAAAGACATCTGGAATATTTTCCTGGAGAAAAATGCGCCTCTGAGAGTGAAGATCCCT
GAGATGCTACAGGCTGAAATTGACTCGCGCCTGCGGAACAGCGAAGATGCCCGTGGTGTT
CTCTGTGAAGCTCAAGAGGCAGCCATGCCTGAGATCCAAGAGCAGATCCACGACTACAGA
ACGAAGCGCACACTGGGGCTGGGCAGCCTGTATGGTGAAAATGACCTGCTGGACCTGGAT
GGGGACCCTCTCCGAGAGCGCCAAGTGGCTGAGAAGCAGCTGGCTGCCCTTGGAGATATT
TTGTCCAAGTATGAGGAAGACAGGAGCGCCCCCATGGACTTCGCCCTCAATACCTACATG
AGCCATGCTGGGATCCGTCTTCGAGAGGCACGACCTTCCAACACAGCTGAAAAGGCCCAG
TCTGCTCCTGACAAGGACAAGTGGCTACCGTTCTTCCCTAAGACCAAGAAGAGCAGCAAT
TCCAAGAAAGAAAAGGATGCCTTGGAGGACAAGAAGCGAAACCCTATCCTCAAATACATT
GGGAAGCCCAAAAGCTCTTCTCAAAGCACATTTCATATTCCCTTGTCCCCTGTGGAAGTC
AAACCAGGCAATGTGAGGAACATCATTCAGCACTTTGAGAACAACCAGCAGTATGATGCC
CCAGAACCTGGGACACAACGACTCTCGACCGGAAGCTTTCCTGAGGACCTGCTGGAGAGT
GACAGTTCACGCTCAGAGATTCGCCTGGGCCGCTCTGAAAGCCTCAAGGGCCGGGAAGAG
ATGAAACGGTCTCGAAAGGCAGAGAACGTGCCCCGCTCTCGCAGTGATGTTGACATGGAT
GCTGCTGCGGAGGCTACTCGCCTGCACCAGTCAGCCTCGTCCTCTACCTCCAGCCTCTCC
ACCAGGTCTCTTGAGAACCCAACCCCTCCATTCACTCCCAAAATGGGCCGCAGGAGCATT
GAGTCCCCCAGTTTGGGGTTCTGCACAGATACCCTCCTTCCCCACCTCCTAGAGGATGAT
CTGGGCCAGCTGTCTGACCTGGAGCCAGAGCCAGATGCCCAAAATTGGCAGCATACAGTG
GGCAAGGATGTGGTGGCTGGGCTAACCCAGCGGGAGATTGACCGGCAAGAGGTCATCAAT
GAGCTGTTTGTGACTGAAGCTTCCCACCTGCGCACACTCCGGGTCCTGGACCTGATCTTC
TACCAGCGAATGAAGAAGGAGAACCTGATGCCCCGGGAGGAGCTGGCCCGGCTCTTCCCG
AACCTGCCTGAACTCATAGAGATTCACAATTCCTGGTGTGAAGCCATGAAGAAGCTCCGG
GAGGAAGGCCCCATCATCAAAGAGATCAGTGACCTCATGCTGGCCCGGTTTGATGGCCCT
GCCCGAGAGGAACTCCAGCAAGTGGCTGCACAGTTCTGTTCCTATCAGTCAATAGCCCTA
GAGCTAATCAAGACCAAGCAACGCAAGGAGAGTCGATTCCAGCTCTTCATGCAGGAGGCT
GAGAGCCACCCTCAGTGTCGGCGGCTGCAGCTGAGAGACCTCATCATCTCTGAGATGCAG
CGGCTCACCAAGTACCCGCTGCTGCTGGAGAGCATCATCAAGCACACAGAGGGTGGCACC
TCTGAGCATGAGAAGCTGTGCCGGGCCCGGGACCAGTGCCGGGAGATTCTCAAGTATGTG
AATGAAGCGGTAAAACAAACAGAGAACCGCCACCGTTTAGAGGGCTACCAGAAACGCCTG
GATGCCACCGCCCTGGAGAGGGCCAGCAACCCCCTGGCAGCAGAGTTCAAGAGCCTGGAT
CTTACAACCAGAAAAATGATCCATGAGGGACCCCTGACCTGGAGGATCAGCAAGGATAAG
ACCTTGGACCTCCACGTGCTGCTGCTGGAGGACCTCCTAGTGCTGCTACAGAAACAGGAT
GAGAAGCTATTGCTGAAGTGCCACAGCAAGACTGCTGTGGGCTCCTCAGACAGCAAGCAG
ACCTTCAGCCCCGTGCTCAAGCTCAATGCTGTGCTCATCCGCTCTGTGGCCACAGATAAA
CGGGCCTTCTTCATCATCTGCACCTCCAAGCTGGGCCCACCCCAGATCTATGAGCTGGTT
GCATTGACGTCATCAGACAAGAACACATGGATGGAGCTCTTAGAAGAGGCCGTGCGGAAT
GCCACCAGGCACCCCGGAGCTGCCCCAATGCCCGTCCATCCTCCACCCCCAGGTCCCCGG
GAGCCAGCCCAGCAGGGCCCCACACCCAGCAGGGTAGAACTGGATGACTCAGACGTGTTC
CATGGTGAACCTGAACCTGAGGAGCTGCCTGGAGGCACTGGGTCCCAGCAGAGGGTCCAA
GGGAAGCACCAGGTCCTGCTAGAGGACCCTGAGCAGGAGGGCAGTGCAGAGGAAGAGGAA
CTGGGTGTCCTGCCTTGCCCTTCCACATCCCTGGATGGAGAGAACAGGGGCATCAGGACA
AGGAACCCCATCCACTTGGCCTTCCCAGGCCCTCTGTTCATGGAAGGGCTCGCTGACTCC
GCTCTGGAAGATGTGGAGAACCTGCGACATCTGATCCTGTGGAGCCTGCTGCCAGGTCAC
ACCATGGAAACTCAGGCTGCCCAGGAGCCCGAGGACGACCTGACACCCACACCTTCTGTC
ATCAGCGTCACCTCTCACCCCTGGGACCCAGGCTCCCCAGGGCAAGCACCCCCTGGGGGT
GAAGGGGACAACACCCAGCTTGCAGGGCTGGAGGGGGAACGGCCAGAGCAGGAAGACATG
GGTCTCTGTTCTCTGGAACACCTACCCCCAAGGACCAGAAATTCTGGGATATGGGAGTCT
CCAGAACTGGACAGGAATCTGGCTGAAGATGCTTCAAGCACAGAGGCAGCAGGAGGTTAC
AAAGTTGTGAGAAAAGCTGAGGTGGCAGGCAGCAAGGTTGTCCCTGCACTACCAGAGAGT
GGCCAGTCAGAGCCTGGGCCACCTGAAGTGGAAGGCGGAACAAAGGCTACGGGGAACTGC
TTTTATGTCAGCATGCCATCAGGACCCCCGGACTCAAGCACCGACCACTCAGAGGCACCC
ATGAGCCCCCCTCAGCCTGACAGCCTCCCTGCAGGGCAGACAGAGCCTCAGCCTCAGCTG
CAGGGAGGCAACGATGATCCAAGACGCCCCAGCCGCTCTCCTCCAAGCCTGGCCCTCAGG
GACGTGGGCATGATCTTCCATACCATTGAGCAGCTCACTCTCAAGCTCAACAGGCTCAAG
GATATGGAGCTGGCCCACAGAGAGCTGCTCAAGTCCCTTGGGGGAGAGTCATCTGGTGGC
ACCACGCCTGTGGGCAGTTTCCACACAGAAGCAGCTAGATGGACAGATGGCTCCCTCTCA
CCTCCCGCTAAGGAGCCCCTAGCTTCTGACTCCAGGAACAGCCATGAACTGGGGCCCTGC
CCTGAGGATGGCTCTGACGCCCCCCTGGAAGACAGCACAGCAGACGCAGCCGCGTCACCA
GGACCATTG
Protein sequence predicted based on nucleotide sequence
MSVRLPQSIDRLSSLSSLGDSAPERKSPSHHRQPSDASETTGLVQRCVIIQKDQHGFGFT VSGDRIVLVQSVRPGGAAMKAGVKEGDRIIKVNGTMVTNSSHLEVVKLIKSGAYVALTLL GSSPSSMGISGLQQDPSPAGAPRITSVIPSPPPPPPLPPPQRITGPKPLQDPEVQKHATQ ILRNMLRQEEKELQRICEVYSRNPASLLEEQIEGARRRVTQLQLKIQQETGGSVDILPLY GDTSQRPSEGRLSLDSQEGDSGLDSGTERFPSLSESLMNRNSVLSDPGLDSPRTSPVIMA RVAQHHRRQGSDAAVPSTGDQGVDQSPKPLIIGPEEDYDPGYFNNESDIIFQDLEKLKSR PAHLGVFLRYIFSQADPSPLLFYLCAEVYQQASPKDSRSLGKDIWNIFLEKNAPLRVKIP EMLQAEIDSRLRNSEDARGVLCEAQEAAMPEIQEQIHDYRTKRTLGLGSLYGENDLLDLD GDPLRERQVAEKQLAALGDILSKYEEDRSAPMDFALNTYMSHAGIRLREARPSNTAEKAQ SAPDKDKWLPFFPKTKKSSNSKKEKDALEDKKRNPILKYIGKPKSSSQSTFHIPLSPVEV KPGNVRNIIQHFENNQQYDAPEPGTQRLSTGSFPEDLLESDSSRSEIRLGRSESLKGREE MKRSRKAENVPRSRSDVDMDAAAEATRLHQSASSSTSSLSTRSLENPTPPFTPKMGRRSI ESPSLGFCTDTLLPHLLEDDLGQLSDLEPEPDAQNWQHTVGKDVVAGLTQREIDRQEVIN ELFVTEASHLRTLRVLDLIFYQRMKKENLMPREELARLFPNLPELIEIHNSWCEAMKKLR EEGPIIKEISDLMLARFDGPAREELQQVAAQFCSYQSIALELIKTKQRKESRFQLFMQEA ESHPQCRRLQLRDLIISEMQRLTKYPLLLESIIKHTEGGTSEHEKLCRARDQCREILKYV NEAVKQTENRHRLEGYQKRLDATALERASNPLAAEFKSLDLTTRKMIHEGPLTWRISKDK TLDLHVLLLEDLLVLLQKQDEKLLLKCHSKTAVGSSDSKQTFSPVLKLNAVLIRSVATDK RAFFIICTSKLGPPQIYELVALTSSDKNTWMELLEEAVRNATRHPGAAPMPVHPPPPGPR EPAQQGPTPSRVELDDSDVFHGEPEPEELPGGTGSQQRVQGKHQVLLEDPEQEGSAEEEE LGVLPCPSTSLDGENRGIRTRNPIHLAFPGPLFMEGLADSALEDVENLRHLILWSLLPGH TMETQAAQEPEDDLTPTPSVISVTSHPWDPGSPGQAPPGGEGDNTQLAGLEGERPEQEDM GLCSLEHLPPRTRNSGIWESPELDRNLAEDASSTEAAGGYKVVRKAEVAGSKVVPALPES GQSEPGPPEVEGGTKATGNCFYVSMPSGPPDSSTDHSEAPMSPPQPDSLPAGQTEPQPQL QGGNDDPRRPSRSPPSLALRDVGMIFHTIEQLTLKLNRLKDMELAHRELLKSLGGESSGG TTPVGSFHTEAARWTDGSLSPPAKEPLASDSRNSHELGPCPEDGSDAPLEDSTADAAASP GPL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
