Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860229
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5229nts
Open reading frame: 1 to 5229
ATGACGTCAAGTTCGCCCGCTGGCTTGGAAGGTTCAGACCTATCTTCCATCAACACCATG
ATGTCGGCGGTCATGAGTGTAGGGAAGGTCACAGAGAATGGCGGGAGCCCCCAGGGGATC
AAGTCCCCCTCGAAGCCTCCAGGACCAAATCGGATTGGCAGAAGGAACCAGGAAACGAAA
GAGGAGAAGTCTTCCTATAACTGCCCCCTGTGTGAGAAGATTTGCACTACCCAGCACCAG
CTGACCATGCACATTCGCCAGCACAACACAGACACTGGAGGAGCCGACCACTCATGCAGC
ATCTGCGGAAAGTCACTGAGCTCGGCCAGCTCCCTCGATCGCCACATGCTGGTGCACTCT
GGCGAGAGGCCTTACAAGTGCACTGTGTGTGGCCAGTCATTTACCACCAATGGGAACATG
CACAGACATATGAAGATCCATGAGAAGGACCCTAACAGTGCCACAGCCACAGCCCCTCCA
TCTCCTCTGAAACGTAGGCGATTGTCCTCCAAGAGGAAACTGAGTCACGATGCCGAGTCA
GAGAGAGAAGACCCAGCACCAGCTAAAAAGATGGTAGAAGACGGGCAGTCAGGTGACTTG
GAGAAGAAAGCTGATGAAGTCTTTCACTGCCCAGTATGTTTCAAGGAGTTTGTTTGCAAG
TATGGACTGGAGACCCACATGGAGACCCATTCAGATAACCCACTAAGATGTGACATTTGT
TGTGTCACCTTTCGAACACATCGAGGACTGCTGCGTCACAACGCGCTTGTCCACAAACAA
CTTCCCAGGGATGCAATGGGCAGACCTTTCATACAGAACAACCCTTCAATTCCTGCTGGC
TTCCACGACTTAGGATTCACGGACTTCTCCTGTAGGAAGTTTCCTCGCATTTCTCAGGCC
TGGTGCGAAACAAACCTGCGGAGGTGCATCAGCGAGCAACACCGTTTTGTCTGCGACACC
TGTGACAAGGCGTTCCCCATGCTCTGCTCACTGGCTCTGCACAAGCAGACCCATGTGGCG
GCAGACCAGGGTCAAGAAAAGCCGCAGGCCACGCCCCTGCCTGGTGACGCCCTGGACCAG
AAGGGCTTCCTGGCCTTGCTTGGCCTGCAGCACACCAAAGACGTCAGGCCTGCCCCCGCC
GAGGAGCCCCTGCCGGATGACAACCAGGCAATTCAGCTCCAGACACTCAAGTGTCAGCTA
CCTCAGGACCCCGGCTGCACCAACCTGCTGAGCCTGTCACCTTTCGAAGCTGCTTCCCTA
GGCGGTTCTCTCACAGTTCTCCCCGCGACCAAGGACAGCATAAAGCACCTGTCCCTGCAG
CCCTTCCAGAAGGGCTTCATCATCCAGCCTGACAGCAGCATTGTGGTCAAGCCCATCTCT
GGCGAGTCGGCCATCGAGCTGGCAGACATCCAGCAAATTCTGAAGATGGCAGCCTCGGCT
CCCCCTCAGATCAGTCTTCCGCCCTTCTCCAAGGCCCCTGCCGCCCCACTGCAGGCGATC
TTCAAGCACATGCCCCCTCTGAAGCCAAAGCCCCTGGTCACACCACGGACGGTGGTGGCC
ACCTCCACGCCCCCGCCTCTCATCAACGCCCAGCAGGCTTCCCCGGGCTGTATCAGCCCC
AGCCTGCCGCCACCGCCCCTGAAGCTCCTCAAAGGCTCAGTGGAGGCGGCCTCCAACGCC
CACCTGCTGCAGTCCAAGTCCGGGACCCAGCCCCACGCGGCCACGCGGCTCTCCCTGCAG
CAGCCGCGGGCGGAGCTGCCGGGCCAGCCTGAGATGAAGACGCAGCTGGAGCAGGACAGC
ATCATCGAGGCCCTGCTGCCGCTGAGCATGGAGGCCAAGATCAAGCAGGAGATCACAGAG
GGGGAACTCAAGGCCTTCATGACAGCGCCCGGCGGCAAGAAGACGCCCGCCATGCGCAAG
GTGCTCTACCCCTGCCGCTTCTGCAACCAGGTGTTTGCCTTCTCGGGGGTCTTGCGTGCC
CACGTGCGCTCCCACCTGGGCATCTCGCCATACCAGTGCAACATCTGCGACTACATCGCC
GCCGACAAGGCCGCGCTCATCCGCCACCTGCGCACGCACAGTGGGGAGCGGCCCTACATT
TGCAAGATCTGCCACTACCCCTTCACTGTCAAAGCCAACTGCGAGCGGCACCTGCGCAAG
AAGCACCTCAAGGCCACCCGCAAGGATATCGAGAAGAACATCGAGTATGTGAGTAGCAGC
GCGGCCGAGCTGGTGGACGCCTTCTGCGCCCCGGACACCGTGTGCCGGCTGTGCGGCGAG
GACCTCAAGCACTATCGTGCCCTGCGCATCCACATGCGCACGCACTGCGGCCGCGGCCTG
GGCGGGGGCCACAAGGGCCGCAAGCCCTTCGAGTGCAAGGAGTGCAGCGCCGCGTTCGCG
GCCAAGCGCAACTGCATCCACCACATCCTCAAGCAGCACCTGCACGTGCCCGAGCAGGAC
ATCGAGAGCTACGTGCTGGCCGCCGACGGCCTGGGCCCCGCAGAGGCGCCGGCCGCTGAG
GCGTCGGGGCGCGGGGAGGACAGTGGCTGCGCTGCCCTTGGTGACTGCAAGCCCCTCACT
GCCTTCCTGGAACCCCAGAACGGCTTTCTTCACAGGGGCCCCACCCAGCCTCCACCTCCC
CATGTCTCGATCAAGTTGGAGCCCGCCAGTAGCTTTGCGGTGGACTTCAATGAGCCCCTG
GACTTCTCGCAGAAGGGCCTGGCCCTGGTCCAAGTGAAGCAGGAAAACATCTCCTTTCTG
AGCCCTTCTTCCCTGGTCCCCTATGACTGCTCCATGGAGCCCATCGACCTGTCCATCCCC
AAGAACTTCAGGAAAGGGGACAAGGATTTGGCCACTCCCAGCGAAGCCAAGAAGCCTGAG
GAGGAGGCGGGGAGCAGCGAGCAGCCCTCTCCCTGCCCAGCACCCGGCCCTTCTCTTCCT
GTAACTTTGGGGCCCAGCGGAATCCTGGAAAGCCCCATGGCCCCTGCTCCGGCGGCCACC
CCGGAACCCCCAGCACAGCCCCTGCAGGGCCCTGTTCAGCTGGCGGTCCCAATCTACTCC
TCAGCCCTGGTCAGCAGCCCTCCACTCGTGGGCAGCTCAGCCCTCCTGAGTGGCACAGCC
TTGCTGCGTCCACTGCGGCCCAAGCCCCCGCTGCTTTTGCCAAAGCCCCCCGTGACAGAA
GAGCTGCCCCCGCTGGCCTCCATTGCCCAGATCATCTCATCTGTATCCTCGGCCCCCACC
CTGCTGAAAACCAAGGTGGCGGACCCAGGGCCCGCAAGCACTGGCAGTAACACCACGGCT
TCAGACAGCTTAGGAGGTTCTGTCCCCAAAGCCGCCACCACCGCCACCCCCGCTGCCACC
ACCAGCCCAAAAGAGTCTAGTGAGCCTCCCGCTCCAGCCAGCAGCCCAGAGGCTGCCTCT
CCCACCGAGCAGGGCCCAGCGGGCACGTCGAAGAAGAGGGGCCGGAAAAGGGGGATGAGG
AGCCGACCCCGCGCCAACAGCGGCGGGGTGGACCTGGACTCCAGCGGGGAGTTTGCCAGC
ATCGAGAAGATGCTGGCCACCACAGACACCAACAAGTTCAGTCCGTTTCTGCAGACAGCG
GAGGACAACACTCAGGATGAGGTGGCCGGAGCCCCTGCCGACCACCATGGGCCCAGTGAT
GAAGAGCAGGGCAGTCCCCCAGAAGACAAGCTGCTGAGGGCCAAGCGGAACTCGTACACC
AACTGCCTGCAGAAGATCACCTGTCCCCACTGTCCCCGGGTTTTCCCTTGGGCCAGCTCC
CTACAGAGGCACATGCTCACACACACTGGTCAGAAACCCTTCCCTTGTCAAAAATGCGAT
GCCTTCTTTTCTACCAAATCTAACTGTGAACGCCACCAGTTGCGCAAACACGGAGTTACC
ACCTGTTCCCTGAGAAGAAACGGGCTTATCCCCCAGTCAAAAGAGAGTGATGTTGGATCC
CATGATAGCACAGACAGTCAGTCGGATGCGGAGACTGCAGCCGCCGCGGGCGAAGTGCTA
GACCTCACCTCACGGGACAGAGAGCAGCCGTCGGAGGGCGCCACTGAGCTCCGCCAGGTC
GCAGGGGATGCGCCTGTGGAGCAGGCCACGGCGGAAACGGCCTCGCCGGTGCACCGGGAA
GAGCACGGGCGTGGGGAGAGCCATGAGCCGGAGGAGGAGCATGGCACTGAGGAGAGCACT
GGGGACGCCGACGGCGCGGAAGAGGACGCGTCGAGCAACCAGAGCCTGGACCTGGACTTC
GCCACCAAGCTCATGGACTTCAAGCTGGCGGAGGGCGACGGCGAGGCAGGCGCCGGGGGC
GCGGCCTCGCAGGAGCAGAAGCTCGCCTGCGACACCTGTGGGAAGAGCTTCAAGTTCCTG
GGCACCCTGAGCCGCCACCGGAAGGCGCACGGCCGCCAGGAGCCCAAGGACGAGAAGGGA
GATGGCGCCAGCACTGCAGAGGAGGGGCCCCAGCCCGCCCCTGAACAGGAGGAGAAGCCC
CCCGAGACCCCGGCAGAGGTGGTGGAGTCGGCCCCGGGTGCCGGGGAGGCCCCGGCGGAA
AAGCTCGCGGAGGAGACGGAGGGCCCCTCCGACGGGGAGAGCGCGGCCGAGAAAAGGTCC
TCAGAGAAGAGCGACGATGACAAGAAACCAAAGACAGACTCCCCCAAAAGCGTGGCCAGC
AAGGCAGACAAGAGGAAGAAGGTCTGCAGCGTGTGCAACAAGCGGTTCTGGTCGCTGCAG
GACCTGACCCGGCACATGCGCTCCCACACAGGGGAAAGGCCATACAAATGTCAGACCTGC
GAGCGAACCTTCACCTTGAAGCACAGCCTGGTTCGCCACCAGCGGATCCACCAGAAAGCC
AGGCATGCCAAACACCACGGGAAGGACAGCGACAAGGAAGAGCGGGGTGAGGAGGACAGC
GAGAATGAGTCCACCCACAGCGGCAACAACGCCGTCTCAGAGAACGAGGCTGAGCTGGCT
CCCAATGCCAGCAACCACATGGCTGTCACCCGGAGCCGGAAGGAGGGCTTGGCCAGTGCC
ACCAAGGACTGCAGCCACAGGGAGGAGAAGGTCACGGCAGGGTGGCCGTCTGAGCCTGGC
CAGGGTGACCTTAACCCAGAGAGCCCGGCGGCCCTGGGGCAGGACCTGCTGGAGCCGCGC
AGCAAGAGGCCTGCCCACCCAATCCTGGCCACAGCTGATGGCGCCTCCCAGCTCGTGGGG
ATGGAGTTG
Protein sequence predicted based on nucleotide sequence
MTSSSPAGLEGSDLSSINTMMSAVMSVGKVTENGGSPQGIKSPSKPPGPNRIGRRNQETK EEKSSYNCPLCEKICTTQHQLTMHIRQHNTDTGGADHSCSICGKSLSSASSLDRHMLVHS GERPYKCTVCGQSFTTNGNMHRHMKIHEKDPNSATATAPPSPLKRRRLSSKRKLSHDAES EREDPAPAKKMVEDGQSGDLEKKADEVFHCPVCFKEFVCKYGLETHMETHSDNPLRCDIC CVTFRTHRGLLRHNALVHKQLPRDAMGRPFIQNNPSIPAGFHDLGFTDFSCRKFPRISQA WCETNLRRCISEQHRFVCDTCDKAFPMLCSLALHKQTHVAADQGQEKPQATPLPGDALDQ KGFLALLGLQHTKDVRPAPAEEPLPDDNQAIQLQTLKCQLPQDPGCTNLLSLSPFEAASL GGSLTVLPATKDSIKHLSLQPFQKGFIIQPDSSIVVKPISGESAIELADIQQILKMAASA PPQISLPPFSKAPAAPLQAIFKHMPPLKPKPLVTPRTVVATSTPPPLINAQQASPGCISP SLPPPPLKLLKGSVEAASNAHLLQSKSGTQPHAATRLSLQQPRAELPGQPEMKTQLEQDS IIEALLPLSMEAKIKQEITEGELKAFMTAPGGKKTPAMRKVLYPCRFCNQVFAFSGVLRA HVRSHLGISPYQCNICDYIAADKAALIRHLRTHSGERPYICKICHYPFTVKANCERHLRK KHLKATRKDIEKNIEYVSSSAAELVDAFCAPDTVCRLCGEDLKHYRALRIHMRTHCGRGL GGGHKGRKPFECKECSAAFAAKRNCIHHILKQHLHVPEQDIESYVLAADGLGPAEAPAAE ASGRGEDSGCAALGDCKPLTAFLEPQNGFLHRGPTQPPPPHVSIKLEPASSFAVDFNEPL DFSQKGLALVQVKQENISFLSPSSLVPYDCSMEPIDLSIPKNFRKGDKDLATPSEAKKPE EEAGSSEQPSPCPAPGPSLPVTLGPSGILESPMAPAPAATPEPPAQPLQGPVQLAVPIYS SALVSSPPLVGSSALLSGTALLRPLRPKPPLLLPKPPVTEELPPLASIAQIISSVSSAPT LLKTKVADPGPASTGSNTTASDSLGGSVPKAATTATPAATTSPKESSEPPAPASSPEAAS PTEQGPAGTSKKRGRKRGMRSRPRANSGGVDLDSSGEFASIEKMLATTDTNKFSPFLQTA EDNTQDEVAGAPADHHGPSDEEQGSPPEDKLLRAKRNSYTNCLQKITCPHCPRVFPWASS LQRHMLTHTGQKPFPCQKCDAFFSTKSNCERHQLRKHGVTTCSLRRNGLIPQSKESDVGS HDSTDSQSDAETAAAAGEVLDLTSRDREQPSEGATELRQVAGDAPVEQATAETASPVHRE EHGRGESHEPEEEHGTEESTGDADGAEEDASSNQSLDLDFATKLMDFKLAEGDGEAGAGG AASQEQKLACDTCGKSFKFLGTLSRHRKAHGRQEPKDEKGDGASTAEEGPQPAPEQEEKP PETPAEVVESAPGAGEAPAEKLAEETEGPSDGESAAEKRSSEKSDDDKKPKTDSPKSVAS KADKRKKVCSVCNKRFWSLQDLTRHMRSHTGERPYKCQTCERTFTLKHSLVRHQRIHQKA RHAKHHGKDSDKEERGEEDSENESTHSGNNAVSENEAELAPNASNHMAVTRSRKEGLASA TKDCSHREEKVTAGWPSEPGQGDLNPESPAALGQDLLEPRSKRPAHPILATADGASQLVG MEL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
