Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860306
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4974nts
Open reading frame: 1 to 4974
ATGTCCGCCGCAGACGAGGTTGACGGGCTGGGCGTGGCCCGGCCGCACTATGGCTCTGTC
CTGGATAATGAAAGACTTACTGCAGAGGAGATGGATGAAAGGAGACGTCAGAACGTGGCT
TATGAGTACCTTTGTCATTTGGAAGAAGCGAAGAGGTGGATGGAAGCATGCCTAGGGGAA
GATCTGCCTCCCACCACAGAACTGGAGGAGGGGCTTAGGAATGGGGTCTACCTTGCCAAA
CTGGGGAACTTCTTCTCTCCCAAAGTAGTGTCCCTGAAAAAAATCTATGATCGAGAACAG
ACCAGATACAAGGCGACTGGCCTCCACTTTAGACACACTGATAATGTGATTCAGTGGTTG
AATGCCATGGATGAGATTGGATTGCCTAAGATTTTTTACCCAGAAACTACAGATATCTAT
GATCGAAAGAACATGCCAAGATGTATCTACTGTATCCATGCACTCAGTTTGTACCTGTTC
AAGCTAGGCCTGGCCCCTCAGATTCAAGACCTATATGGAAAGGTTGACTTCACAGAAGAA
GAAATCAACAACATGAAGACTGAGTTGGAGAAGTATGGCATCCAGATGCCTGCCTTTAGC
AAGATTGGGGGCATCTTGGCTAATGAACTGTCAGTGGATGAAGCCGCATTACATGCTGCT
GTTATTGCTATTAATGAAGCTATTGACCGTAGAATTCCAGCCGACACATTTGCAGCTTTG
AAAAATCCGAATGCCATGCTTGTAAATCTTGAAGAGCCCTTGGCATCCACTTACCAGGAT
ATACTTTACCAGGCTAAGCAGGACAAAATGACAAATGCTAAAAACAGGACAGAAAACTCA
GAGAGAGAAAGAGATGTTTATGAGGAGCTGCTCACGCAAGCTGAAATTCAAGGCAATATA
AACAAAGTCAATACATTTTCTGCATTAGCAAATATCGACCTGGCTTTAGAACAAGGAGAT
GCACTGGCCTTGTTCAGGGCTCTGCAGTCACCAGCCCTGGGGCTTCGAGGACTGCAGCAA
CAGAATAGCGACTGGTACTTGAAGCAGCTCCTGAGTGATAAACAGCAGAAGAGACAGAGT
GGTCAGACTGACCCCCTGCAGAAGGAGGAGCTGCAGTCTGGAGTGGATGCTGCAAACAGT
GCTGCCCAGCAATATCAGAGAAGATTGGCAGCAGTAGCACTGATTAATGCTGCAATCCAG
AAGGGTGTTGCTGAGAAGACTGTTTTGGAACTGATGAATCCCGAAGCCCAGCTGCCCCAG
GTGTATCCATTTGCCGCCGATCTCTATCAGAAGGAGCTGGCTACCCTGCAGCGACAAAGT
CCTGAACATAATCTCACCCACCCAGAGCTCTCTGTCGCAGTGGAGATGTTGTCATCGGTG
GCCCTGATCAACAGGGCATTGGAATCAGGAGATGTGAATACAGTGTGGAAGCAATTGAGC
AGTTCAGTTACTGGTCTTACCAATATTGAGGAAGAAAACTGTCAGAGGTATCTCGATGAG
TTGATGAAACTGAAGGCTCAGGCACATGCAGAGAATAATGAATTCATTACATGGAATGAT
ATCCAAGCTTGCGTGGACCATGTGAACCTGGTGGTGCAAGAGGAACATGAGAGGATTTTA
GCCATTGGTTTAATTAATGAAGCCCTGGATGAAGGTGATGCCCAAAAGACTCTGCAGGCC
CTACAGATTCCTGCAGCTAAACTTGAGGGAGTCCTTGCAGAAGTGGCCCAGCATTACCAA
GACACGCTGATTAGAGCGAAGAGAGAGAAAGCCCAGGAAATCCAGGATGAGTCAGCTGTG
TTATGGTTGGATGAAATTCAAGGTGGAATCTGGCAGTCCAACAAAGACACCCAAGAAGCA
CAGAAGTTTGCCTTAGGAATCTTTGCCATTAATGAGGCAGTAGAAAGTGGTGATGTTGGC
AAAACACTGAGTGCCCTTCGCTCCCCTGATGTTGGCTTGTATGGAGTCATCCCTGAGTGT
GGTGAAACTTACCACAGTGATCTTGCTGAAGCCAAGAAGAAAAAACTGGCAGTAGGAGAT
AATAACAGCAAGTGGGTGAAGCACTGGGTAAAAGGTGGATATTATTATTACCACAATCTG
GAGACCCAGGAAGGAGGATGGGATGAACCTCCAAATTTTGTGCAAAATTCTATGCAGCTT
TCTCGGGAGGAGATCCAGAGTTCTATCTCTGGGGTGACTGCCGCATATAACCGAGAACAG
CTGTGGCTGGCCAATGAAGGCCTGATCACCAGGCTGCAGGCTCGCTGCCGTGGATACTTA
GTTCGACAGGAATTCCGATCCAGGATGAATTTCCTGAAGAAACAAATCCCTGCCATCACC
TGCATTCAGTCACAGTGGAGAGGATACAAGCAGAAGAAGGCATATCAAGATCGGTTAGCT
TACCTGCGCTCCCACAAAGATGAAGTTGTAAAGATTCAGTCCCTGGCAAGGATGCACCAA
GCTCGAAAGCGCTATCGAGATCGCCTGCAGTACTTCCGGGACCATATAAATGACATTATC
AAAATCCAGGCTTTTATTCGGGCAAACAAAGCTCGGGATGACTACAAGACTCTCATCAAT
GCTGAGGATCCTCCTATGGTTGTGGTCCGAAAATTTGTCCACCTGCTGGACCAAAGTGAC
CAGGATTTTCAGGAGGAGCTTGACCTTATGAAGATGCGGGAAGAGGTTATCACCCTCATT
CGTTCTAACCAGCAGCTGGAGAATGACCTCAATCTCATGGATATCAAAATTGGACTGCTA
GTGAAAAATAAGATTACGTTGCAGGATGTGGTTTCCCACAGTAAAAAACTTACCAAAAAA
AATAAGGAACAGTTGTCTGATATGATGATGATAAATAAACAGAAGGGAGGTCTCAAGGCT
TTGAGCAAGGAGAAGAGAGAGAAGTTGGAAGCTTACCAGCACCTGTTTTATTTATTGCAA
ACCAATCCCACCTATCTGGCCAAGCTCATTTTTCAGATGCCCCAGAACAAGTCCACCAAG
TTCATGGACTCTGTAATCTTCACACTCTACAACTACGCGTCCAACCAGCGAGAGGAGTAC
CTGCTCCTGCGGCTCTTTAAGACAGCACTCCAAGAGGAAATCAAGTCGAAGGTAGATCAG
ATTCAAGAGATTGTGACAGGAAATCCTACGGTTATTAAAATGGTTGTAAGTTTCAACCGT
GGTGCCCGTGGCCAGAATGCCCTGAGACAGATCTTGGCCCCAGTCGTGAAGGAAATTATG
GATGACAAATCTCTCAACATCAAAACTGACCCTGTGGATATTTACAAATCTTGGGTTAAT
CAGATGGAGTCTCAGACAGGAGAGGCAAGCAAACTGCCCTATGATGTGACCCCTGAGCAG
GCGCTAGCTCATGAAGAAGTGAAGACACGGCTAGACAGCTCCATCAGGAACATGCGGGCT
GTGACAGACAAGTTTCTCTCAGCCATTGTCAGCTCTGTGGACAAAATCCCTTATGGGATG
CGCTTCATTGCCAAAGTGCTGAAGGACTCGTTGCATGAGAAGTTCCCTGATGCTGGTGAG
GATGAGCTGCTGAAGATTATTGGTAACTTGCTTTATTATCGATACATGAATCCAGCCATT
GTTGCTCCTGATGCCTTTGACATCATTGACCTGTCAGCAGGAGGCCAGCTTACCACAGAC
CAACGCCGAAATCTGGGCTCCATTGCAAAAATGCTTCAGCATGCTGCTTCCAATAAGATG
TTTCTGGGAGATAATGCCCACTTAAGCATCATTAATGAATATCTTTCCCAGTCCTACCAG
AAATTCAGACGGTTTTTCCAAACTGCTTGTGATGTCCCAGAGCTTCAGGATAAATTTAAT
GTGGATGAGTACTCTGATTTAGTAACCCTCACCAAACCAGTAATCTACATTTCCATTGGT
GAAATCATCAACACCCACACTCTCCTGTTGGATCACCAGGATGCCATTGCTCCGGAGCAC
AATGATCCAATCCACGAACTGCTGGACGACCTCGGCGAGGTGCCCACCATCGAGTCCCTG
ATAGGGGAAAGCTCTGGCAATTTAAATGACCCAAATAAGGAGGCACTGGCTAAGACGGAA
GTGTCTCTCACCCTGACCAACAAGTTCGACGTGCCTGGAGATGAGAATGCAGAAATGGAT
GCTCGAACCATCTTACTGAATACAAAACGTTTAATTGTGGATGTCATCCGGTTCCAGCCA
GGAGAGACCTTGACTGAAATCCTAGAAACACCAGCCACCAGTGAACAGGAAGCAGAACAT
CAGAGAGCCATGCAGAGACGTGCTATCCGTGATGCCAAAACACCTGACAAGATGAAAAAG
TCAAAATCTGTAAAGGAAGACAGCAACCTCACTCTTCAAGAGAAGAAAGAGAAGATCCAG
ACAGGTTTAAAGAAGCTAACAGAGCTTGGAACCGTGGACCCAAAGAACAAATACCAGGAA
CTGATCAACGACATTGCCAGGGATATTCGGAATCAGCGGAGGTACCGACAGAGGAGAAAG
GCCGAACTAGTGAAACTGCAACAGACATACGCTGCTCTGAACTCTAAGGCCACCTTTTAT
GGGGAGCAGGTGGATTACTATAAAAGCTATATCAAAACCTGCTTGGATAACTTAGCCAGC
AAGGGCAAAGTCTCCAAAAAGCCTAGGGAAATGAAAGGAAAGAAAAGCAAAAAGATTTCT
CTGAAATATACAGCAGCAAGACTACATGAAAAAGGAGTTCTTCTGGAAATTGAGGACCTG
CAAGTGAATCAGTTTAAAAATGTTATATTTGAAATCAGTCCAACAGAAGAAGTTGGAGAC
TTCGAAGTGAAAGCCAAATTCATGGGAGTTCAAATGGAGACTTTTATGTTACATTATCAG
GACCTGCTGCAGCTACAGTATGAAGGAGTTGCAGTCATGAAATTATTTGATAGAGCTAAA
GTAAATGTCAACCTCCTGATCTTCCTTCTCAACAAAAAGTTCTACGGGAAGTTG
Protein sequence predicted based on nucleotide sequence
MSAADEVDGLGVARPHYGSVLDNERLTAEEMDERRRQNVAYEYLCHLEEAKRWMEACLGE DLPPTTELEEGLRNGVYLAKLGNFFSPKVVSLKKIYDREQTRYKATGLHFRHTDNVIQWL NAMDEIGLPKIFYPETTDIYDRKNMPRCIYCIHALSLYLFKLGLAPQIQDLYGKVDFTEE EINNMKTELEKYGIQMPAFSKIGGILANELSVDEAALHAAVIAINEAIDRRIPADTFAAL KNPNAMLVNLEEPLASTYQDILYQAKQDKMTNAKNRTENSERERDVYEELLTQAEIQGNI NKVNTFSALANIDLALEQGDALALFRALQSPALGLRGLQQQNSDWYLKQLLSDKQQKRQS GQTDPLQKEELQSGVDAANSAAQQYQRRLAAVALINAAIQKGVAEKTVLELMNPEAQLPQ VYPFAADLYQKELATLQRQSPEHNLTHPELSVAVEMLSSVALINRALESGDVNTVWKQLS SSVTGLTNIEEENCQRYLDELMKLKAQAHAENNEFITWNDIQACVDHVNLVVQEEHERIL AIGLINEALDEGDAQKTLQALQIPAAKLEGVLAEVAQHYQDTLIRAKREKAQEIQDESAV LWLDEIQGGIWQSNKDTQEAQKFALGIFAINEAVESGDVGKTLSALRSPDVGLYGVIPEC GETYHSDLAEAKKKKLAVGDNNSKWVKHWVKGGYYYYHNLETQEGGWDEPPNFVQNSMQL SREEIQSSISGVTAAYNREQLWLANEGLITRLQARCRGYLVRQEFRSRMNFLKKQIPAIT CIQSQWRGYKQKKAYQDRLAYLRSHKDEVVKIQSLARMHQARKRYRDRLQYFRDHINDII KIQAFIRANKARDDYKTLINAEDPPMVVVRKFVHLLDQSDQDFQEELDLMKMREEVITLI RSNQQLENDLNLMDIKIGLLVKNKITLQDVVSHSKKLTKKNKEQLSDMMMINKQKGGLKA LSKEKREKLEAYQHLFYLLQTNPTYLAKLIFQMPQNKSTKFMDSVIFTLYNYASNQREEY LLLRLFKTALQEEIKSKVDQIQEIVTGNPTVIKMVVSFNRGARGQNALRQILAPVVKEIM DDKSLNIKTDPVDIYKSWVNQMESQTGEASKLPYDVTPEQALAHEEVKTRLDSSIRNMRA VTDKFLSAIVSSVDKIPYGMRFIAKVLKDSLHEKFPDAGEDELLKIIGNLLYYRYMNPAI VAPDAFDIIDLSAGGQLTTDQRRNLGSIAKMLQHAASNKMFLGDNAHLSIINEYLSQSYQ KFRRFFQTACDVPELQDKFNVDEYSDLVTLTKPVIYISIGEIINTHTLLLDHQDAIAPEH NDPIHELLDDLGEVPTIESLIGESSGNLNDPNKEALAKTEVSLTLTNKFDVPGDENAEMD ARTILLNTKRLIVDVIRFQPGETLTEILETPATSEQEAEHQRAMQRRAIRDAKTPDKMKK SKSVKEDSNLTLQEKKEKIQTGLKKLTELGTVDPKNKYQELINDIARDIRNQRRYRQRRK AELVKLQQTYAALNSKATFYGEQVDYYKSYIKTCLDNLASKGKVSKKPREMKGKKSKKIS LKYTAARLHEKGVLLEIEDLQVNQFKNVIFEISPTEEVGDFEVKAKFMGVQMETFMLHYQ DLLQLQYEGVAVMKLFDRAKVNVNLLIFLLNKKFYGKL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
