Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860348
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 7173nts
Open reading frame: 1 to 7173
ATGAGCAGCACGCTGTCACCCACAGACTTTGACAGCTTGGAAATCCAGGGCCAGTACAGT
GACATCAACAACCGCTGGGACCTTCCTGACTCGGACTGGGACAATGACAGCAGCTCGGCC
CGCCTCTTTGAGAGGTCTCGCATTAAGGCTCTGGCAGATGAACGAGAAGCTGTGCAGAAG
AAAACCTTCACCAAGTGGGTAAACTCGCACCTGGCCCGGGTCACGTGCCGGGTGGGGGAC
CTGTACAGCGACCTCCGGGACGGACGCAACCTGCTGAGGCTCCTCGAGGTGCTCTCGGGA
GAGATACTGCCAAAGCCTACAAAGGGCCGCATGCGGATCCACTGCCTGGAGAACGTGGAC
AAGGCACTGCAGTTCCTCAAGGAGCAGAAAGTGCACTTGGAAAACATGGGCTCCCATGAC
ATTGTGGACGGAAACCACCGACTGACCCTTGGGCTGGTCTGGACCATCATCCTTCGATTC
CAGATCCAAGACATCAGTGTGGAGACAGAAGACAACAAGGAGAAGAAGTCAGCCAAGGAT
GCCCTGCTTCTGTGGTGCCAGATGAAGACTGCAGGTTATCCCAACGTCAATGTACACAAC
TTCACCACCAGCTGGAGAGATGGACTAGCTTTCAACGCCATCGTGCATAAACACCGGCCA
GACCTGCTGGATTTTGAGTCTCTGAAGAAGTGTAATGCACACTATAATCTGCAGAATGCA
TTCAATCTGGCTGAAAAGGAACTGGGACTTACCAAGCTGCTGGATCCCGAAGACGTGAAT
GTGGACCAGCCAGATGAGAAGTCAATCATTACCTATGTGGCTACTTACTACCATTACTTC
TCCAAGATGAAGGCCCTGGCCGTGGAAGGCAAGAGAATTGGCAAGGTGCTGGACCATGCC
ATGGAGGCAGAGCGCCTGGTGGAGAAATACGAGTCCCTGGCCTCGGAGCTGCTGCAGTGG
ATCGAGCAAACGATCGTGACCCTCAATGACCGGCAGTTGGCCAACTCCCTTAGCGGGGTC
CAGAACCAGCTGCAGTCCTTCAACTCCTACCGCACCGTGGAGAAGCCGCCCAAGTTTACC
GAGAAAGGGAACTTGGAAGTGCTGCTCTTCACCATCCAGAGCAAGCTTCGGGCCAACAAC
CAGAAGGTCTACACGCCCCGCGAGGGCCGGCTCATCTCGGACATCAACAAGGCTTGGGAG
CGGCTGGAGAAGGCGGAGCACGAGCGTGAGCTGGCCCTGCGCACCGAGCTCATCCGCCAG
GAGAAGCTGGAGCAGCTGGCCGCCCGCTTCGACCGCAAGGCTGCCATGCGGGAGACCTGG
CTCAGCGAGAACCAGCGCCTCGTGTCCCAGGACAACTTTGGGCTGGAGCTGGCAGCTGTC
GAGGCAGCAGTACGGAAGCACGAAGCCATTGAGACGGACATCGTGGCCTACAGCGGCCGG
GTGCAGGCAGTGGACGCCGTGGCTGCAGAGCTGGCCGCCGAGCGCTACCACGACATCAAG
CGCATCGCCGCTCGGCAGCACAACGTGGCACGGCTCTGGGACTTCTTGCGGCAGATGGTG
GCCGCCCGGCGGGAGCGGCTCCTCCTCAACCTGGAGCTGCAGAAGGTGTTCCAGGACCTG
CTCTACCTCATGGACTGGATGGAAGAGATGAAGGGCCGGCTGCAGTCTCAGGACCTGGGC
AGGCACCTAGCAGGAGTGGAGGACCTGCTGCAGCTGCACGAGCTGGTGGAGGCAGACATC
GCCGTGCAGGCCGAGAGGGTGCGGGCCGTCAGCGCCTCTGCCCTGCGCTTCTGCAACCCA
GGGAAAGAGTATAGACCTTGCGACCCGCAGCTGGTGTCGGAGCGGGTGGCCAAGCTAGAG
CAGAGCTATGAGGCACTGTGCGAGTTGGCAGCGGCGCGGCGGGCCCGGCTGGAGGAATCA
CGGCGGCTCTGGCGTTTCCTCTGGGAGGTGGGTGAAGCTGAGGCCTGGGTGCGGGAGCAG
CAGCACCTCCTGGCCTCAGCCGACACGGGCCGAGACCTGACCGGTGCCCTCCGCCTGCTC
AACAAGCACACAGCCCTGCGGGGCGAGATGAGCGGCCGGCTGGGGCCCCTGAAGCTCACC
CTGGAGCAGGGCCAGCAGTTGGTGGCCGAGGGTCACCCTGGGGCAAGCCAGGCCTCTGCC
CGTGCAGCTGAACTCCAAGCCCAGTGGGAGCGGCTAGAGGCCCTGGCCGAGGAGCGTGCC
CAGCGGCTGGCCCAAGCCGCCAGCCTCTACCAGTTCCAGGCCGATGCAAACGACATGGAG
GCCTGGTTGGTTGACGCACTGCGCCTGGTGTCCAGCCCCGAGCTGGGGCACGACGAGTTC
TCCACGCAGGCTCTAGCCAGGCAGCATCGGGCCCTGGAGGAGGAGATTCGAAGCCACCGG
CCAACCCTGGACGCCTTGAGGGAACAGGCAGCAGCCCTGCCCCCCACACTGAGCCGCACG
CCCGAGGTGCAGGGCCGGGTGCCCACCCTGGAGCGGCACTACGAGGAGCTGCAGGCCCGG
GCAGGCGAGCGAGCGCGGGCCTTGGAGGCAGCCCTGGCGCTCTACACCATGCTCAGCGAG
GCCGGGGCCTGTGGACTCTGGGTGGAGGAGAAGGAGCAGTGGCTCAACGGGCTGGCCCTG
CCTGAACGCCTGGAGGACCTGGAGGTCGTGCAGCAGAGGTTCGAGACCCTGGAGCCTGAA
ATGAACACCCTTGCAGCACAAATCACCGCGGTGAATGACATTGCCGAGCAGTTACTGAAG
GCCAACCCCCCAGGCAAAGACCGCATTGTCAACACCCAGGAGCAGCTCAACCACAGGTGG
CAGCAGTTTCGGCGTCTGGCAGACGGCAAGAAGGCAGCTCTCACCTCAGCCCTGAGCATC
CAGAACTACCACTTAGAGTGCACGGAGACCCAGGCCTGGATGAGAGAGAAGACCAAAGTC
ATCGAGTCCACCCAGGGCCTAGGCAACGATCTGGCTGGGGTGCTGGCCCTGCAGCGCAAG
CTGGCCGGCACGGAGCGGGACCTGGAGGCCATCGCCGCCCGGGTGGGCGAACTGACTCGA
GAGGCAAATGCCCTGGCTGCCGGCCATCCCGCTCAGGCAGTGGCCATCAACGCCCGGCTG
AGAGAGGTGCAGACCGGCTGGGAGGACCTCAGGGCCACCATGCGGCGTCGAGAAGAGTCG
CTGGGGGAGGCGCGGCGGCTGCAGGACTTCTTGCGCAGCTTGGATGACTTCCAGGCCTGG
CTAGGCCGCACTCAGACTGCTGTGGCCTCTGAAGAAGGGCCGGCCACCCTGCCTGAGGCA
GAGGCCCTCCTGGCCCAACATGCAGCCCTGCGGGGAGAGGTGGAGCGGGCCCAGAGCGAG
TATAGCCGGCTGCGAGCCCTGGGCGAGGAGGTGACCCGGGACCAGGCTGACCCCCAGTGC
CTCTTCCTACGACAGCGACTGGAGGCCCTGGGAACTGGCTGGGAGGAGCTGGGCCGAATG
TGGGAGAGCCGGCAAGGTCGCCTGGCCCAGGCCCACGGCTTCCAGGGATTCCTGCGGGAT
GCTCGTCAGGCTGAGGGCGTGCTCAGCAGCCAGGAATATGTTCTGTCTCACACGGAGATG
CCAGGGACACTCCAGGCTGCTGATGCTGCCATTAAAAAACTGGAGGACTTCATGAGCACC
ATGGACGCCAATGGGGAACGGATCCACGGGCTCCTGGAGGCTGGCCGCCAGCTGGTATCT
GAAGGCAACATCCACGCCGACAAGATTCGGGAAAAGGCAGACTCCATTGAGAGGAGGCAC
AAGAAGAATCAAGACGCAGCGCAGCAATTTCTGGGCCGTCTTCGGGACAACCGGGAGCAG
CAGCATTTCCTGCAAGATTGTCACGAGCTGAAGCTCTGGATCGACGAGAAGATGCTGACA
GCCCAGGACGTGTCCTATGACGAGGCCCGCAACCTGCATACTAAGTGGCAGAAGCACCAG
GCATTCATGGCCGAGCTGGCTGCCAACAAAGACTGGCTGGACAAGGTGGACAAGGAAGGG
CGAGAGCTCACCCTTGAGAAGCCAGAGCTGAAAGCCCTGGTGTCGGAGAAGCTGAGAGAC
CTGCACAGGCGCTGGGACGAGCTGGAGACCACCACCCAAGCCAAGGCCCGCAGCCTCTTT
GATGCCAACCGAGCTGAGCTGTTTGCCCAGAGCTGCTGTGCCCTGGAGAGCTGGCTGGAG
AGCCTGCAGGCCCAGCTGCACTCGGATGACTACGGCAAGGACCTCACCAGCGTCAACATC
CTGCTCAAGAAGCAGCAGATGCTGGAATGGGAGATGGCTGTGAGAGAGAAGGAGGTGGAG
GCAATCCAGGCCCAGGCCAAAGCACTGGCCCAGGAGGACCAGGGTGCAGGGGAGGTGGAG
AGAACCTCGAGGGCCGTGGAGGAGAAGTTCAGGGCCTTGTGCCAGCCCATGCGGGAACGC
TGCCGGCGCCTGCAGGCTTCTCGCGAGCAGCACCAGTTCCACCGCGATGTGGAAGATGAG
ATTTTGTGGGTGACAGAGCGGCTGCCCATGGCCAGCTCCATGGAGCATGGCAAGGACCTG
CCCAGCGTCCAGCTTCTCATGAAGAAAAACCAGACCCTGCAGAAAGAGATTCAGGGCCAT
GAGCCCCGGATCGCGGACCTGAGGGAGCGGCAGCGTGCTCTAGGTGCAGCAGCAGCAGGT
CCAGAGCTGGCTGAGCTGCAGGAAATGTGGAAACGCCTGGGCCACGAGCTGGAACTTCGA
GGGAAGCGACTGGAGGATGCCCTGCGAGCCCAGCAGTTCTACCGCGATGCCGCCGAGGCG
GAGGCCTGGATGGGCGAGCAGGAATTACACATGATGGGCCAGGAGAAGGCCAAGGATGAG
CTGAGTGCCCAGGCAGAGGTGAAGAAGCACCAGGTGCTGGAGCAAGCCCTGGCCGACTAC
GCGCAGACCATCCACCAGCTGGCGGCCAGCAGCCAGGACATGATTGACCACGAGCACCCA
GAGAGCACTCGGATATCCATCCGCCAAGCCCAGGTGGACAAGCTGTATGCCGGCCTGAAG
GAGCTGGCTGGAGAGCGGCGGGAGCGCCTGCAGGAGCACCTCCGGCTGTGCCAGCTCCGC
CGCGAGCTGGATGACCTGGAACAGTGGATCCAGGAGCGCGAGGTGGTGGCGGCCTCCCAC
GAGCTGGGCCAGGACTACGAGCATGTGACTATGCTCCGAGACAAATTCCGAGAGTTCTCC
CGGGACACAAGCACCATCGGTCAGGAGCGCGTAGATAGCGCCAATGCGCTGGCCAATGGG
CTCATTGCTGGGGGCCATGCTGCACGGGCCACCGTGGCCGAGTGGAAGGACAGTCTCAAC
GAGGCCTGGGCTGACCTGCTTGAGCTGCTGGACACACGGGGTCAGGTGCTGGCCGCGGCG
TACGAGCTGCAGCGCTTCCTGCACGGGGCACGCCAAGCCCTGGCGCGGGTGCAGCACAAG
CAGCAGCAGCTTCCGGACGGGACTGGCCGCGACCTCAACGCTGCCGAGGCCCTGCAGCGC
CGACACTGTGCCTACGAGCATGACATTCAGGCCCTCAGCCCCCAGGTCCAGCAGGTGCAG
GACGACGGCCACCGGCTCCAGAAGGCCTACGCTGGAGACAAGGCTGAGGAGATCGGCCGC
CACATGCAGGCCGTGGCCGAGGCCTGGGCCCAGCTTCAGGGAAGCTCTGCCGCCCGCCGG
CAGCTGCTGCTGGACACCACAGACAAGTTCCGCTTCTTCAAGGCTGTCCGGGAACTGATG
CTCTGGATGGATGAGGTCAACCTGCAGATGGATGCCCAGGAGCGTCCCCGGGATGTGTCC
TCCGCGGATCTAGTCATCAAGAACCAGCAAGGCATCAAGGCAGAGATAGAGGCCCGGGCA
GACCGCTTCTCCTCCTGCATCGACATGGGGAAGGAGCTGCTGGCCAGGAGCCACTATGCG
GCCGAGGAGATCTCAGAGAAGCTGTCTCAGCTGCAGGCACGGCGCCAGGAGACAGCTGAG
AAGTGGCAGGAGAAGATGGACTGGCTTCAGCTGGTTTTGGAGGTGCTTGTGTTTGGAAGA
GATGCAGGGATGGCAGAGGCCTGGCTCTGCAGCCAGGAGCCACTGGTGCGCAGCGCTGAG
CTGGGTTGCACGGTCGACGAAGTTGAGAGCCTCATCAAGCGGCACGAGGCCTTCCAGAAG
TCAGCAGTGGCCTGGGAGGAGCGATTCTGTGCGCTGGAGAAGCTTACTGCGCTAGAGGAG
CGGGAGAAGGAGCGAAAGAGAAAGAGGGAGGAGGAGGAGCGGCGGAAACAGCCGCCTGCT
CCCGAACCCACAGCCAGTGTGCCTCCAGGGGACCTGGTGGGCGGCCAGACAGCTTCTGAC
ACCACCTGGGACGGAACCCAGCCACGGCCACCACCATCCACACAAGCACCCAGTGTTAAT
GGAGTCTGCACAGATGGAGAGCCCTCACAGCCCCTGCTGGGACAACAGAGACTTGAGCAC
AGCAGCTTCCCCGAAGGGCCGGGACCTGGCTCAGGGGACGAAGCCAATGGGCCCCGGGGA
GAGAGGCAGACCCGGACTCGGGGCCCGGCCCCATCTGCAATGCCCCAGAGCAGGTCTACC
GAGTCAGCCCATGCTGCCACCCTGCCGCCTCGAGGCCCAGAGCCATCTGCCCAGGAGCAG
ATGGAGGGGATGCTGTGCCGCAAGCAGGAGATGGAGGCCTTCGGGAAGAAGGCTGCCAAC
AGGTCCTGGCAGAACGTGTACTGTGTCCTGCGGCGTGGGAGCCTCGGCTTTTACAAGGAT
GCCAAGGCAGCCAGCGCGGGAGTGCCATACCACGGAGAAGTGCCTGTCAGCCTGGCCAGG
GCCCAGGGCAGCGTCGCCTTTGATTACCGAAAGCGCAAACATGTCTTCAAGCTGGGCTTA
CAGGATGGAAAAGAATATTTATTCCAGGCCAAGGATGAGGCAGAGATGAGCTCGTGGCTA
CGGGTGGTGAATGCAGCCATTGCCACAGCGTCTTCTGCCTCTGGAGAGCCTGAAGAGCCG
GTGGTGCCCAGCACCACCCGGGGCATGACCCGGGCCATGACCATGCCCCCAGTGTCACCC
GTCGGGGCTGAGGGGCCTGTTGTGCTCCGCAGCAAAGACGGCAGAGAACGAGAGCGAGAA
AAACGCTTCAGCTTCTTTAAGAAGAACAAGTTG
Protein sequence predicted based on nucleotide sequence
MSSTLSPTDFDSLEIQGQYSDINNRWDLPDSDWDNDSSSARLFERSRIKALADEREAVQK KTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGEILPKPTKGRMRIHCLENVD KALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKD ALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLLDFESLKKCNAHYNLQNA FNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAVEGKRIGKVLDHA MEAERLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSYRTVEKPPKFT EKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQ EKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGR VQAVDAVAAELAAERYHDIKRIAARQHNVARLWDFLRQMVAARRERLLLNLELQKVFQDL LYLMDWMEEMKGRLQSQDLGRHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCNP GKEYRPCDPQLVSERVAKLEQSYEALCELAAARRARLEESRRLWRFLWEVGEAEAWVREQ QHLLASADTGRDLTGALRLLNKHTALRGEMSGRLGPLKLTLEQGQQLVAEGHPGASQASA RAAELQAQWERLEALAEERAQRLAQAASLYQFQADANDMEAWLVDALRLVSSPELGHDEF STQALARQHRALEEEIRSHRPTLDALREQAAALPPTLSRTPEVQGRVPTLERHYEELQAR AGERARALEAALALYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPE MNTLAAQITAVNDIAEQLLKANPPGKDRIVNTQEQLNHRWQQFRRLADGKKAALTSALSI QNYHLECTETQAWMREKTKVIESTQGLGNDLAGVLALQRKLAGTERDLEAIAARVGELTR EANALAAGHPAQAVAINARLREVQTGWEDLRATMRRREESLGEARRLQDFLRSLDDFQAW LGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSEYSRLRALGEEVTRDQADPQC LFLRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEM PGTLQAADAAIKKLEDFMSTMDANGERIHGLLEAGRQLVSEGNIHADKIREKADSIERRH KKNQDAAQQFLGRLRDNREQQHFLQDCHELKLWIDEKMLTAQDVSYDEARNLHTKWQKHQ AFMAELAANKDWLDKVDKEGRELTLEKPELKALVSEKLRDLHRRWDELETTTQAKARSLF DANRAELFAQSCCALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEWEMAVREKEVE AIQAQAKALAQEDQGAGEVERTSRAVEEKFRALCQPMRERCRRLQASREQHQFHRDVEDE ILWVTERLPMASSMEHGKDLPSVQLLMKKNQTLQKEIQGHEPRIADLRERQRALGAAAAG PELAELQEMWKRLGHELELRGKRLEDALRAQQFYRDAAEAEAWMGEQELHMMGQEKAKDE LSAQAEVKKHQVLEQALADYAQTIHQLAASSQDMIDHEHPESTRISIRQAQVDKLYAGLK ELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFS RDTSTIGQERVDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAA YELQRFLHGARQALARVQHKQQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSPQVQQVQ DDGHRLQKAYAGDKAEEIGRHMQAVAEAWAQLQGSSAARRQLLLDTTDKFRFFKAVRELM LWMDEVNLQMDAQERPRDVSSADLVIKNQQGIKAEIEARADRFSSCIDMGKELLARSHYA AEEISEKLSQLQARRQETAEKWQEKMDWLQLVLEVLVFGRDAGMAEAWLCSQEPLVRSAE LGCTVDEVESLIKRHEAFQKSAVAWEERFCALEKLTALEEREKERKRKREEEERRKQPPA PEPTASVPPGDLVGGQTASDTTWDGTQPRPPPSTQAPSVNGVCTDGEPSQPLLGQQRLEH SSFPEGPGPGSGDEANGPRGERQTRTRGPAPSAMPQSRSTESAHAATLPPRGPEPSAQEQ MEGMLCRKQEMEAFGKKAANRSWQNVYCVLRRGSLGFYKDAKAASAGVPYHGEVPVSLAR AQGSVAFDYRKRKHVFKLGLQDGKEYLFQAKDEAEMSSWLRVVNAAIATASSASGEPEEP VVPSTTRGMTRAMTMPPVSPVGAEGPVVLRSKDGREREREKRFSFFKKNKL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
