Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860354
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5346nts
Open reading frame: 1 to 5346
ATGAGATTTTATTTTCAAGATAAAGCTGCTGGAAACTTTGCAACAAAATGTATTCGGGTC
TCTAGTACTGCCACCACTCAAGATGTAATCGAAACGCTCGCGGAGAAATTTCGACCTGAT
ATGCGAATGCTGTCCTCTCCCAAGTATTCACTCTATGAAGTGCATGTCAGCGGAGAAGAA
AGAAGATTGGATATAGATGAGAAACCTCTAGTTGTACAACTGAATTGGAACAAAGATGAT
CGGGAAGGCAGATTTGTTCTTAAGAATGAGAATGACGCCATTCCTCCTAAGAAGGCTCAA
AGTAATGGACCTGAAAAGCAGGAAAAAGAAGGGGTTATCCAGAACTTCAAGAGAACTCTC
TCAAAGAAAGAAAAGAAGGAAAAAAAGAAGAGAGAAAAAGAGGCATTGCGACAGGCATCT
GATAAAGATGATAGACCTTTCCAAGGGGAGGATGTTGAAAATTCTCGACTGGCTGCTGAG
GTTTACAAAGACATGCCGGAAACCAGCTTTACTCGAACCATTTCTAATCCTGAGGTGGTT
ATGAAACGACGGAGGCAGCAAAAATTGGAAAAGAGAATGCAGGAATTTCGGAGCTCAGAT
GGGCGGCCTGATTCAGGTGGAACATTGAGAATTTATGCAGATAGTTTAAAACCAAATATT
CCCTACAAGACAATCCTGCTGTCTACTACAGATCCTGCAGACTTTGCTGTGGCTGAAGCT
TTAGAGAAGTATGGTCTGGAAAAAGAAAACCCTAAGGATTACTGCATCGCCCGGGTTATG
CTTCCTCCTGGAGCCCAGCATTCTGATGAAAAGGGTGCTAAAGAAATTATTCTTGATGAT
GATGAGTGTCCTTTACAAATCTTCAGGGAATGGCCAAGTGACAAAGGGATTTTAGTCTTT
CAGTTGAAGAGGAGGCCACCAGACCACATCCCAAAGAAAACCAAGAAACACTTGGAAGGC
AAGACACCCAAGGGAAAGGAGAGAGCTGACGGGTCTGGCTATGGCTCCACCCTTCCTCCG
GAGAAGCTGCCCTATTTAGTAGAGTTAAGCCCAGGGAGAAGGAATCACTTTGCCTACTAC
AACTATCACACTTACGAAGATGGTTCTGACTCTAGAGATAAGCCAAAGCTTTACCGCCTT
CAGTTAAGTGTTACTGAAGTTGGGACAGAAAAGTTGGATGACAACTCTATCCAGTTGTTT
GGCCCAGGAATTCAGCCCCATCACTGTGACCTTACCAACATGGATGGAGTGGTCACTGTG
ACGCCCAGAAGTATGGACGCAGAAACCTACGTGGAAGGCCAGCGCATCTCAGAAACCACC
ATGCTGCAGAGTGGCATGAAAGTGCAGTTTGGGGCGTCCCATGTATTTAAGTTTGTGGAC
CCCAGTCAGGATCATGCTCTTGCAAAAAGATCTGTGGATGGAGGCCTGATGGTTAAGGGC
CCAAGACATAAACCTGGAATTGTTCAGGAGACAACTTTTGATTTGGGAGGAGATATTCAT
AGTGGGACAGCATTACCGACAAGCAAGAGCACCACTAGGCTGGACAGCGACAGAGTGTCG
TCTGCCTCTAGCACAGCCGAGCGGGGAATGGTGAAGCCGATGATCAGAGTAGAACAGCAG
CCAGATTATCGCAGGCAAGAAAGCAGAACACAGGATGCTTCTGGGCCTGAGCTGATACTA
CCTGCAAGCATTGAATTCAGGGAAAGTTCTGAAGATTCATTTTTGTCTGCCATTATAAAT
TATACTAATAGCTCTACAGTCCACTTTAAGTTGTCCCCTACATATGTATTATATATGGCA
TGCCGGTATGTATTGTCCAACCAGTACAGACCTGACATCAGCCCTACAGAGCGCACACAT
AAAGTCATTGCAGTCGTCAACAAGATGGTGAGCATGATGGAGGGTGTCATCCAGAAACAG
AAGAATATTGCAGGGGCACTTGCCTTCTGGATGGCAAATGCATCTGAACTTCTCAACTTC
ATTAAGCAAGACCGAGACCTTAGTCGGATCACACTGGATGCTCAAGATGTTTTAGCACAT
TTGGTTCAAATGGCATTTAAATACTTGGTTCACTGTCTTCAATCAGAACTTAATAATTAC
ATGCCAGCCTTTCTAGATGACCCTGAAGAGAACAGTCTGCAACGACCAAAAATAGATGAT
GTGCTGCACACGCTCACAGGAGCCATGTCCTTGCTACGACGCTGCAGAGTCAATGCCGCC
CTGACCATCCAGCTCTTCTCTCAGCTCTTCCACTTCATCAATATGTGGCTGTTCAATAGA
TTGGTGACCGACCCAGATTCGGGGCTGTGCTCCCATTACTGGGGTGCGATTATCCGTCAG
CAGTTGGGCCATATTGAAGCCTGGGCTGAGAAGCAGGGGCTGGAACTGGCTGCGGACTGT
CATCTGAGCAGGATCGTGCAGGCAACGACTTTGCTTACCATGGATAAGTATGCACCTGAT
GACATTCCAAATATAAACAGCACCTGCTTTAAGTTAAATTCATTACAACTTCAAGCCTTA
TTACAGAACTATCACTGTGCACCTGATGAGCCTTTTATCCCAACGGATCTTATAGAAAAT
GTAGTGACTGTGGCTGAAAACACTGCCGATGAGCTGGCCCGCAGTGATGGAAGGGAAGTG
CAGTTGGAGGAGGATCCTGATCTGCAGCTGCCGTTTCTTTTGCCAGAAGATGGTTATTCT
TGTGATGTTGTCAGAAACATTCCAAATGGTTTACAAGAATTTTTAGACCCTCTGTGCCAG
AGAGGATTTTGCAGGTTAATTCCTCACACACGTTCACCAGGTACTTGGACAATATATTTT
GAAGGTGCAGATTATGAAAGTCACCTTCTGCGTGAGAACACAGAGCTGGCTCAGCCTCTG
AGGAAAGAACCTGAAATAATCACTGTGACCCTAAAAAAGCAGAATGGAATGGGCCTTAGC
ATTGTTGCAGCAAAGGGTGCTGGTCAAGATAAACTAGGAATCTATGTGAAGTCGGTTGTG
AAAGGAGGTGCTGCAGATGTGGATGGACGTCTAGCTGCAGGTGATCAGCTCCTCAGTGTG
GATGGACGAAGTCTGGTTGGACTCTCTCAGGAAAGGGCGGCAGAACTCATGACAAGAACA
AGCTCTGTGGTGACACTGGAAGTAGCAAAGCAGGGTGCCATCTACCACGGTCTGGCCACC
CTTCTCAATCAGCCATCCCCCATGATGCAGAGAATTTCAGATCGTCGTGGCTCAGGTAAA
CCCCGACCAAAGAGTGAAGGCTTTGAGCTCTATAATAATTCAACTCAAAATGGGTCTCCT
GAGAGTCCTCAGCTGCCTTGGGCAGAATATAGTGAACCAAAGAAATTGCCTGGTGATGAC
AGACTGATGAAAAATAGAGCTGATCACCGTTCCAGCCCCAACGTAGCAAATCAGCCTCCT
AGTCCTGGAGGGAAAAGTGCATATGCCTCTGGAACAACAGCGAAGATAACATCTGTCTCT
ACTGGAAACCTCTGCACTGAGGAGCAGACGCCTCCGCCTAGACCTGAAGCCTACCCCATC
CCCACTCAGACGTACACCAGAGAGTATTTTACCTTCCCAGCTTCCAAATCCCAGGATCGG
ATGGCTCCTCCTCAGAACCAGTGGCCAAATTATGAGGAAAAGCCACATATGCACACAGAT
AGTAATCATTCCAGTATTGCAATTCAGCGTGTTACACGTTCCCAAGAAGAACTTCGAGAA
GATAAAGCTTACCAACTTGAGCGGCATCGAATAGAGGCAGCTATGGACCGAAAGTCTGAT
AGTGATATGTGGATAAATCAGAGCTCCTCACTGGACTCCAGTACCTCTAGCCAGGAGCAT
CTGAACCATTCCTCTAAGTCGGTCACCCCTGCTTCCACACTGACCAAAAGTGGCCCTGGC
CGTTGGAAAACACCAGCAGCCATACCGGCCACCCCTGTGGCCGTCTCCCAGCCAATCCGA
ACAGACCTGCCTCCGCCACCCCCGCCACCTCCAGTCCACTATGCCGGTGATTTCGATGGA
ATGTCCATGGATTTGCCTCTCCCACCACCCCCTTCCGCCAACCAGATAGGGCTGCCGTCT
GCGCAGGTGGCTGCTGCTGAACGGAGAAAGAGAGAAGAACATCAGCGTTGGTATGAGAAG
GAGAAGGCCCGCCTGGAGGAGGAGCGGGAGAGGAAGCGGAGAGAGCAGGAGAGGAAGTTG
GGCCAGATGCGCACTCAGTCCTTAAACCCTGCTCCGTTTTCTCCCCTGACTGCACAGCAG
ATGAAGCCCGAAAAGCCTTCCACACTCCAGCGGCCACAGGAAACAGTCATTCGGGAGCTG
CAGCCTCAGCAGCAGCCCCGCACGATCGAGCGCAGAGACTTGCAGTACATTACAGTCAGC
AAAGAGGAGCTTTCCTCGGGGGACAGTCTGTCCCCCGACCCGTGGAAGCGGGACGCCAAG
GAGAAGCTGGAGAAGCAGCAGCAGATGCACATCGTGGACATGCTGAGCAAGGAGATCCAG
GAGCTCCAGAGCAAACCGGACCGCAGCGCCGAGGAGAGCGACCGGCTGCGCAAGCTCATG
CTGGAGTGGCAGTTCCAGAAGAGACTCCAGGAGTCGAAGCAGAAGGACGAAGATGACGAG
GAGGAGGAGGACGATGATGTGGACACCATGCTGATCATGCAGCGCCTGGAGGCTGAACGA
AGAGCGAGGGACGAGGAGCGGAGGCGGCAGCAGCAGTTAGAAGAGATGCGCAAGCGGGAA
GCGGAAGACCGAGCGAGGCAAGAGGAAGAGCGCCGGCGGCAGGAGGAGGAGCGAACAAAA
CGAGACGCTGAAGAAAAGAGGCGACAGGAAGAAGGGTATTACAGCCGCCTGGAAGCCGAG
AGGCGCAGACAGCACGACGAGGCGGCGCGCAGGTTGCTGGAGCCCGAGGCGCCCGGTCTG
TGCCGCCCTCCGCTTCCCCGGGACTACGAGCCCCCGTCCCCGTCCCCCGCGCCCGGCGCC
CCTCCTCCCCCGCCTCAGCGAAACGCCTCCTACCTCAAAACACAGGTCCTCTCCCCCGAC
TCGCTGTTCACTGCCAAGTTTGTTGCATACAATGAGGAGGAGGAGGAGGAGGACTGCAGC
CTAGCAGGACCAAACTCTTACCCAGGATCTACTGGAGCAGCTGTTGGAGCCCATGACGCC
TGTCGGGATGCAAAAGAGAAGCGCTCTAAAAGCCAAGATGCAGATTCACCTGGAAGTTCT
GGGGCCCCTGAAAACTTGACATTCAAGGAACGCCAGCGTCTTTTTTCACAAGGTCAAGAT
GTATCCAATAAAGTGAAAGCTTCTCGTAAATTAACAGAACTGGAGAATGAACTGAACACA
AAGTTG
Protein sequence predicted based on nucleotide sequence
MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEE RRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTL SKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVV MKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEA LEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVF QLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYY NYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTV TPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKG PRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQ PDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMA CRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNF IKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDD VLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQ QLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQAL LQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYS CDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPL RKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSV DGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGK PRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPP SPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDR MAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSD SDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIR TDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEK EKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIREL QPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQ ELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAER RARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAE RRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPD SLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSS GAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTKL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
