Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860361
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4332nts
Open reading frame: 1 to 4332
ATGAGTCTGCTGATGTTTACACAACTACTGCTCTGTGGATTTTTATATGTTCGGGTTGAT
GGATCGCGTCTTCGCCAGGAGGACTTTCCCCCGCGGATTGTGGAGCATCCTTCCGATGTC
ATCGTCTCTAAGGGCGAGCCCACGACTCTGAACTGCAAGGCGGAGGGCCGGCCAACGCCC
ACCATTGAGTGGTACAAAGATGGGGAGCGAGTGGAGACTGACAAGGACGATCCCCGGTCC
CACAGGATGCTTCTGCCCAGCGGATCCTTATTCTTCTTGCGCATCGTGCACGGGCGCAGG
AGTAAACCTGATGAAGGAAGCTACGTTTGTGTTGCGAGGAACTATCTTGGTGAAGCAGTG
AGTCGAAATGCGTCTCTGGAAGTGGCATTGTTACGAGATGACTTCCGACAAAACCCCACA
GATGTTGTAGTGGCAGCTGGAGAGCCTGCAATCCTGGAGTGCCAGCCTCCCCGGGGACAC
CCAGAACCCACCATCTACTGGAAAAAAGACAAAGTTCGAATTGATGACAAGGAAGAAAGA
ATAAGTATCCGTGGTGGAAAACTGATGATCTCCAATACCAGGAAAAGTGATGCAGGGATG
TATACTTGTGTTGGTACCAATATGGTGGGAGAAAGGGACAGTGACCCAGCAGAGCTGACT
GTCTTTGAACGACCCACATTTCTCAGGAGGCCAATTAACCAGGTGGTACTGGAGGAAGAA
GCTGTAGAATTTCGTTGTCAAGTCCAAGGAGATCCTCAACCAACTGTGAGGTGGAAAAAG
GATGATGCAGACTTGCCAAGAGGAAGGTATGACATCAAAGACGATTACACACTAAGAATT
AAAAAGACCATGAGTACAGATGAAGGCACCTATATGTGTATTGCTGAGAATCGGGTTGGA
AAAATGGAAGCCTCTGCTACACTCACCGTCCGAGCTCGCCCTGTTGCTCCCCCACAGTTT
GTGGTTCGGCCAAGAGATCAGATTGTTGCTCAAGGTCGAACAGTGACATTTCCCTGTGAA
ACTAAAGGAAACCCACAGCCAGCTGTTTTTTGGCAGAAAGAAGGCAGCCAGAACCTACTT
TTCCCAAACCAACCCCAGCAGCCCAACAGTAGATGCTCAGTGTCACCAACTGGAGACCTC
ACAATCACCAACATTCAACGTTCCGACGCGGGTTACTACATCTGCCAGGCTTTAACTGTG
GCAGGAAGCATTTTAGCAAAAGCTCAACTGGAGGTTACTGATGTTTTGACAGATAGACCT
CCACCTATAATTCTACAAGGCCCAGCCAACCAAACGCTGGCAGTGGATGGTACAGCGTTA
CTGAAATGTAAAGCCACTGGTGATCCTCTTCCTGTAATTAGCTGGTTAAAGGAGGGATTT
ACTTTTCCGGGTAGAGATCCAAGAGCAACAATTCAAGAGCAAGGCACACTGCAGATTAAG
AATTTACGGATTTCTGATACTGGCACTTATACTTGTGTGGCTACAAGTTCAAGTGGAGAG
ACTTCCTGGAGTGCAGTGCTGGATGTGACAGAGTCTGGAGCAACAATCAGTAAAAACTAT
GATTTAAGTGACCTGCCAGGGCCACCATCCAAACCGCAGGTCACTGATGTTACTAAGAAC
AGTGTCACCTTGTCCTGGCAGCCAGGTACCCCTGGAACCCTTCCAGCAAGTGCATATATC
ATTGAGGCTTTCAGCCAATCAGTGAGCAACAGCTGGCAGACCGTGGCAAACCATGTAAAG
ACCACCCTCTATACTGTAAGAGGACTGCGGCCCAATACAATCTACTTATTCATGGTCAGA
GCGATCAACCCCCAAGGTCTCAGTGACCCAAGTCCCATGTCAGATCCTGTGCGCACACAA
GATATCAGCCCACCAGCACAAGGAGTGGACCACAGGCAAGTGCAGAAAGAGCTAGGAGAT
GTCCTTGTCCGTCTTCATAATCCAGTTGTGCTGACTCCCACCACGGTTCAGGTCACATGG
ACGGTTGATCGCCAACCCCAGTTTATCCAAGGCTACCGAGTGATGTATCGTCAGACTTCA
GGTCTGCAGGCGACATCTTCGTGGCAGAATTTAGATGCCAAAGTCCCGACTGAACGAAGT
GCTGTCTTAGTCAACCTGAAAAAGGGGGTGACTTATGAAATTAAAGTACGGCCATATTTT
AATGAGTTCCAAGGAATGGATAGTGAATCTAAAACGGTTCGTACTACTGAAGAAGCCCCA
AGTGCCCCACCACAGTCTGTCACTGTACTGACAGTTGGAAGCTACAATAGCACAAGTATT
AGTGTTTCCTGGGATCCTCCTCCTCCAGATCACCAGAATGGAATTATCCAAGAATACAAG
ATCTGGTGTCTAGGAAATGAAACGCGATTCCATATCAACAAAACTGTGGATGCAGCCATT
CGGTCCGTAATAATTGGTGGATTATTCCCAGGTATTCAATACCGGGTAGAGGTTGCAGCT
AGTACCAGTGCAGGGGTTGGAGTAAAGAGTGAGCCACAGCCAATAATAATCGGGAGACGC
AATGAAGTTGTCATTACTGAAAACAATAACAGCATAACTGAGCAAATCACTGATGTGGTG
AAGCAACCAGCCTTTATAGCTGGTATTGGTGGTGCCTGCTGGGTAATTCTGATGGGTTTT
AGCATATGGTTGTATTGGCGAAGAAAGAAGAGGAAGGGACTCAGTAATTATGCTGTTACG
TTTCAAAGAGGAGATGGAGGACTAATGAGCAATGGAAGCCGTCCAGGTCTTCTCAATGCT
GGTGATCCCAGCTATCCATGGCTTGCTGATTCTTGGCCAGCCACGAGCTTGCCAGTAAAT
AATAGCAACAGTGGCCCAAATGAGATTGGAAATTTTGGCCGTGGAGATGTGCTGCCACCA
GTTCCAGGCCAAGGGGATAAAACAGCAACGATGCTCTCAGATGGAGCCATTTATAGTAGC
ATTGACTTCACTACCAAAACCAGTTACAACAGTTCCAGCCAAATAACACAGGCTACCCCA
TATGCCACGACACAGATCTTGCATTCCAACAGCATACATGAATTGGCTGTCGATCTGCCT
GATCCACAATGGAAAAGCTCAATTCAGCAAAAAACAGATCTGATGGGATTTGGTTATTCT
CTACCTGATCAGAACAAAGGTAACAATGGTGGGAAAGGTGGAAAAAAGAAGAAAAATAAA
AACTCTTCTAAACCACAGAAAAACAATGGATCCACTTGGGCCAATGTCCCTCTACCTCCC
CCCCCAGTCCAGCCCCTTCCTGGCACGGAGCTGGAACACTATGCAGTGGAACAACAAGAA
AATGGCTATGACAGTGATAGCTGGTGCCCACCATTGCCAGTACAAACTTACTTACACCAA
GGTCTGGAAGATGAACTGGAAGAAGATGATGATAGGGTCCCAACACCTCCTGTTCGAGGC
GTGGCTTCTTCTCCTGCTATCTCCTTTGGACAGCAGTCCACTGCAACTCTTACTCCATCC
CCACGGGAAGAGATGCAACCCATGCTGCAGGCTCACCTGGATGAGTTGACAAGAGCCTAT
CAGTTTGATATAGCAAAACAAACATGGCACATTCAAAGCAATAATCAACCTCCACAGCCT
CCAGTTCCACCGTTAGGTTATGTGTCTGGAGCCTTGATTTCTGATTTGGAAACGGATGTT
GCAGATGATGATGCCGACGACGAAGAGGAAGCTTTAGAAATCCCCAGGCCCCTGAGAGCA
CTGGACCAGACTCCTGGATCCAGCATGGACAATCTAGACAGCTCTGTGACAGGTTCAATG
GTAAATGGATGGGGCTCTGCATCTGATGAGGATCGTAACTTTTCTAGTCATAGATCTAGT
GTAGGTAGCTCCTCAGATGGCTCTATCTTTGCCAGCGGCAGTTTTGCACAAGCACTGGTG
GCAGCAGCAGATAAAGCTGGTTTTAGGCTGGATGGAACCAGCCTTACAAGAACAGGAAAA
GCCTTTACCTCCTCTCAAAGACCTCGACCTACCAGCCCATTTTCTACTGACAGTAACACC
AGTGCAGCCCTGAGTCAAAGTCAGAGGCCTCGGCCCACTAAAAAACACAAGGGAGGGCGG
ATGGACCAACAACCAGCATTGCCTCATCGAAGGGAAGGAATGACAGATGAGGAGGCCTTG
GTGCCCTATAGCAAGCCCAGTTTCCCATCTCCAGGTGGCCACAGCTCATCAGGAACAGCT
TCTTCTAAGGGATCCACTGGACCTAGGAAAACCGAGGTGTTGAGAGCAGGCCACCAGCGC
AATGCCAGCGACCTTCTTGACATAGGATATATGGGCTCCAACAGTCAAGGACAGTTTACA
GGTGAATTATTG
Protein sequence predicted based on nucleotide sequence
MSLLMFTQLLLCGFLYVRVDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTP TIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAV SRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEER ISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEE AVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVG KMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLL FPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRP PPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIK NLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKN SVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVR AINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTW TVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYF NEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYK IWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRR NEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVT FQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPP VPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLP DPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPP PPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRG VASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQP PVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGSM VNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAQALVAAADKAGFRLDGTSLTRTGK AFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEAL VPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFT GELL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
