Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860387
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6468nts
Open reading frame: 1 to 6468
ATGAATTATTCATCATTCCTTAGAATTTGGGTCTCTTTCATCTTCGCACTTGTACAGCAC
CAAGCTCAGCCCAGGGAGCTCATGTATCCATTTTGGCAGAATGACACCAAAACCCCTAAA
GTAGATGATGGAAGCTCATCTGAGATTAAGTTGGCCATCCCAGTTTTCTTCTTTGGCGTT
CCTTACCGCACTGTCTATGTCAATAACAACGGAGTTGTTTCCTTCAATGTGCTAGTGAGC
CAGTTCACGCCAGAATCCTTTCCCCTGACAGATGGGAGAGCCTTCGTCGCCCCATTTTGG
GCAGATGTGCACAATGGAATTCGAGGCGAGATCTATTACAGAGAGACCATGGAGCCTGCC
ATCTTGAAAAGAGCCACCAAGGACATCAGGAAGTACTTCAAAGACATGGCAACCTTCTCT
GCCACTTGGGTTTTCATTGTGACATGGGAGGAAGTCACGTTTTATGGAGGCAGCAGCACC
ACACCTGTGAACACCTTCCAGGCCGTCCTAGTGTCCGATGGCTCCTATACATTCACCCTC
TTCAATTATTACGAAATCAACTGGACCACGGGGACGGCGAGTGGCGGCGACCCCCTGACA
GGTCTTGGTGGAGTGATGGCACAGGCAGGATTTAATGGTGGAAACCTCACCAATTTCTTC
AGCCTCCCGGGGTCAAGAACCCCCGAGATCGTGAATATCCAGGAGACCACAAACGTCAAT
GTTCCAGGCCGCTGGGCATTTAAAGTTGATGGAAAGGAAATTGACCCAGCCAATGGCTGC
ACCTCAAGGGGACAATTCCTTCGGCGAGGGGAGGTGTTTTGGGATGACTTGAACTGCACC
GTCAAGTGCCGCTGTCTGGATTTCAACAATGAGATCTACTGCCAGGAGGCTTCCTGTAGC
CCCTACGAGGTGTGCGAACCCAAAGGCAAATTCTTCTACTGCAGCGCTGTGGAGACCAGC
ACATGCGTGGTGTTTGGGGAGCCACACTACCACACTTTTGACGGCTTCCTCTTCCACTTC
CAAGGCTCCTGTGCCTACTTGCTGGCCCGACAGTGTTTGCAGACTTCCAGCCTCCCTTTC
TTCAGTGTGGAGGCCAAGAATGAACACCGCAGAGGTTCAGCCGTCTCCTGGGTGAAGGAG
CTCTCAGTGGAGGTGAATGGCTACAAGATTCTCATCCCCAAAGGAAGCTATGGAAGAGTC
AAGGTTAATGACCTAGTGACTTCTTTGCCTGTCACCTTAGACTTGGGGACAGTGAAAATC
TACCAGAGTGGCATATCTACTGCCGTGGAAACAGATTTTGGGCTCTTAGTGACTTTTGAT
GGCCAGCACTACGCCTCCATTTCCGTCCCAGGCTCCTATATAAACTCCACCTGTGGACTC
TGTGGAAACTATAATAAAAACCCACTGGATGACTTCCTCCGCCCGGATGGCAGGCCGGCC
ATGTCTGTCCTGGATCTGGGAGAGAGCTGGCGTGTGTACCACGCAGACTGGAAGTGCGAC
TCCGGCTGCGTCGACAACTGCACCCAGTGCGACGCTGCCACTGAAGCCCTCTACTTTGGC
TCTGACTACTGCGGCTTCCTCAACAAGACAGACGGCCCTCTGTGGGAGTGTGGCACTGTC
GTGGACCCCACTGCTTTTGTGCACAGCTGCGTGTATGACCTGTGCAGTGTGAGGGACAAT
GGCACGCTCCTCTGCCAAGCCATCCAGGCCTATGCTCTTGTGTGCCAAGCCCTTGGCATT
CCAATTGGAGACTGGCGAACCCAGACTGGGTGTGTGTCCACAGTGCAGTGCCCGAGCTTC
AGCCACTACTCCGTGTGCACAAGCAGCTGCCCCGACACATGCTCCGACCTGACGGCCTCG
CGGAACTGCGCCACGCCGTGCACAGAGGGCTGCGAGTGCAACCAGGGCTTCGTCCTCAGC
ACCAGCCAGTGCGTCCCTCTGCACAAGTGCGGCTGCGACTTCGACGGCCACTACTACACC
ATGGGGGAGTTCTTCTGGGCCACGGCCAACTGCACTGTGCAATGCCTGTGCGAGGAGGGC
GGGGACGTCTACTGCTTCAACAAGACCTGCGGCAGCGGGGAGGTGTGCGCCGTGGAGGAC
GGCTACCAGGGCTGCTTCCCCAAGCGGGAGACCGTGTGCCTGCTCAGCCAGAACCAGGTG
CTGCACACCTTTGACGGCGCCTCCTACGCCTTCCCCTCCGAGTTCTCCTACACCCTCCTG
AAGACCTGCCCTGAGCGCCCAGAGTACTTGGAAATCGACATCAACAAGAAGAAGCCCGAT
GCAGGACCTGCTTGGCTGCGGGGACTTCGGATCCTGGTGGCCGACCAGGAGGTCAAGATA
GGAGGCATCGGGGCTTCGGAAGTCAAGTTGAATGGTCAGGAAGTGGAATTGCCTTTTTTC
CATCCTTCGGGGAAGCTGGAAATTTATCGAAACAAAAACAGTACGACAGTGGAGTCCAAG
GGCGTGGTGACTGTCCAGTACTCAGACATAGGTCTATTGTACATCCGGCTGTCCACCACA
TACTTCAATTGCACAGGGGGCTTGTGCGGCTTCTACAATGCCAACGCCAGTGACGAGTTC
TGTCTCCCCAACGGCAAGTGCACGGACAACCTGGCAGTGTTCCTGGAAAGCTGGACAACT
TTCGAGGAGATCTGCAATGGAGAGTGTGGGGACCTGCTGAAGGCCTGCAACAATGACTCG
GAGCTGCTCAAGTTTTATCGAAGCCGCTCCAGGTGCGGCATCATCAACGACCCCTCCAAC
AGCTCCTTCCTGGAGTGCCATGGGGTGGTGAACGTCACTGCCTATTACCGCACCTGCCTT
TTCCGCCTGTGCCAGAGTGGGGGCAATGAGTCAGAGCTCTGTGACTCTGTGGCCCGGTAT
GCAAGCGCCTGCAAGAATGCGGACGTGGAGGTGGGGCCCTGGCGGACCTATGACTTCTGC
CCACTGGAGTGCCCAGAGAACAGCCACTTTGAGGAGTGCATCACATGTACAGAGACCTGT
GAGACCCTTACCCTGGGCCCCATCTGCGTGGATAGCTGCTCTGAGGGATGTCAGTGTGAT
GAGGGCTATGCTCTACTGGGCAGCCAGTGTGTCACGCGGAGTGAGTGTGGCTGCAACTTT
GAGGGGCACCAACTTGCCACCAATGAGACCTTCTGGGTGGACCTGGACTGCCAGATCTTC
TGCTATTGCAGTGGCACAGACAACAGGGTCCACTGCGAGACCATTCCCTGCAAGGATGAT
GAGTACTGCATGGAGGAAGGTGGCCTGTACTACTGCCAAGCCCGCACCGACGCCTCCTGC
ATCGTCTCAGGCTACGGCCACTACCTCACCTTTGATGGCTTCCCCTTTGACTTCCAGACC
AGCTGCCCACTCATCCTGTGCACCACAGGAAGCAGGCCAAGCTCAGACTCTTTCCCCAAG
TTTGTTGTCACAGCCAAGAATGAGGACCGGGACCCGTCACTGGCCTTGTGGGTTAAGCAG
GTGGACGTGACCGTGTTTGGCTACAGCATCGTGATCCACCGAGCTTACAAGCACACTGTG
CTGGTCAACAGTGAACGGCTCTATCTGCCCCTGAAGCTGGGGCAAGGGAAGATAAATATC
TTTTCCTTTGGCTTCCACGTGGTGGTGGAAACTGATTTTGGCCTGAAGGTTGTATATGAC
TGGAAGACCTTCCTGTCCATCACAGTCCCTCGGAGCATGCAGAACAGCACCTATGGTCTG
TGTGGCCGCTACAACGGCAACCCTGATGATGACCTGGAGATGCCCATGGGTCTGCTTGCA
TCGAGTGTCAATGAGTTTGGGCAGAGCTGGGTGAAGAGGGACACCTTCTGCCAGGTGGGC
TGTGGGGACCGCTGTCCGTCCTGTGCCAAGGTGGAAGGTTTCTCCAAAGTGCAGCAGCTG
TGCAGCCTGATCCCCAACCAGAACGCTGCCTTCTCCAAGTGTCACAGCAAAGTTAACCCC
ACCTTCTTCTATAAGAACTGCCTGTTTGACTCTTGCATCGATGGGGGCGCGGTGCAGACC
GCCTGCAGCTGGCTGCAGAACTACGCCAGCACCTGCCAGACTCAGGGGATTACGGTGACT
GGCTGGAGGAATTACACGTCCTGCACTGTCACCTGCCCTCCAAACAGCCATTACGAGAGC
TGCGTGAGTGTCTGCCAGCCCCGCTGCGCCGCCATCCGCCTGAAGAGTGACTGCAGCCAC
TACTGCGTGGAGGGCTGTCACTGCGACGCTGGCTACGTCCTCAACGGCAAGAGCTGCATC
CTGCCCCACAGCTGCGGCTGCTACTCCGATGGCAAATATTACGAGCCCAAGCAGCTATTT
TGGAACAGCGACTGCACGCGGCGCTGCCGCTGTTTCCGTCGCAACGTGATTCAGTGCGAC
CCGCGCCAATGCAAGTCAGACGAGGAGTGTGCGCTGCGCAACGGGGTGCGCGGCTGCTTC
AGCACCAAGACCTCCTACTGCCTGGCGGCCGGCGGCGGCGTCTTCCGCACCTTCGACGGC
GCCTTCCTGCGCTTCCCAGCCAACTGCGCCTTCGTGCTGTCCACCATCTGCCAGAAACTG
CCCGACATCTCCTTCCAGCTTATCATCAACTTCGACAAGTGGTCGGCCCCCAACCTCACC
ATCATTTCGCCCGTCTACTTCTACATTAACGAAGAGCAGATTCTCATCAACGACCGGAAC
ACGGTCAAGGTGAATGGCACACAAGTGAATGTTCCATTTATAACTGGTTTGGCAACCAAA
ATCTACAGCAGTGAGGGGTTTCTGGTGATTGACACCAGCCCAGACATCCAGATATACTAC
AATGGTTTCAACGTCATTAAAATCAGCATCAGCGAGAGGCTGCAGAACAAAGTGTGCGGT
CTCTGTGGCAACTTCAACGGGGACCTAACAGATGATTATGTGACCTTGCGAGGGAAGCCG
GTGGTAAGCAGCGTGGTGCTGGCCCAGAGCTGGAAAACCAATGGCATGCAGAAGAGACCT
CTTGCCCCCAGCTGCAACGAGCTGCAGTTCTCACAGTATGCAGCCATGTGTGACAATGTG
CACATCCAGAAGATGCAGGGTGATGGCTACTGCCTGAAGCTCACCGACATGAAGGGCTTC
TTCCAGCCCTGCTATGGGCTTCTCGATCCCCTCCCATTCTACGAGTCCTGCTACCTGGAC
GGCTGCTACAGCCACAAGAAGTTCCAGCTGTGCGGCTCCCTGGCCGCCTACGGGGAGGCC
TGCCGCTCCTTCGGGATCCTTAGCACCGAGTGGATTGAGAAGGAGAATTGCTCAGGAGTG
GTTGAAGATCCCTGTGTGGGGGCGGACTGTCCCAACCGAACTTGCGAGCTGGGCAATGGC
AGGGAGCTGTGTGGCTGCATCGAGCCACCCCCCTATGGAAATAACTCACATGACATTATC
GATGCAGAGGTGACCTGCAAAGCAGCCCAAATGGAAGTGTCCATATCTAAGTGCAAGCTC
TTCCAGCTCGGTTTTGAGAGGGAGGGCGTGAGGATCAATGACAGACAGTGCACCGGCATC
GAGGGGGAAGATTTTATCTCCTTTCAGATCAACAACACCAAAGGGAATTGTGGAAACATT
GTGCAGTCCAATGGCACGCATATCATGTATAAAAACACACTCTGGATCGAAAGCGCCAAC
AACACTGGCAACATCATCACCAGGGACCGCACGATCAATGTGGAATTTTCATGTGCTTAT
GAGCTGGATATCAAGATCTCCTTGGATTCTGTTGTGAAGCCTATGCTAAGTGTAATTAAC
CTGACAGTTCCAACCCAAGAAGGCAGCTTCATCACCAAGATGGCTCTCTACAAAAACGCC
TCCTACAAACATCCTTACCGCCAGGGTGAAGTAGTGTTGACGACTCGAGATGTGCTGTAT
GTAGGGGTTTTTGTGGTTGGAGCTGACGCCACACATTTAATCCTAACACTCAACAAATGC
TATGCCACACCCACCCGAGATAGCAATGATAAGCTCCGATATTTCATCATTGAAGGAGGG
TGTCAGAACCTCAAAGATAACACCATTGGCATCGAGGAGAATGCAGTCTCCCTGACCTGT
CGCTTTCACGTCACCGTCTTTAAATTCATAGGGGATTACGACGAAGTTCACCTTCACTGT
GCAGTGTCACTCTGCGACTCAGAAAAGTACTCCTGTAAAATCACTTGCCCACACAATTCC
AGGATTGCCACAGATTACACAAAAGAGCCCAAAGAACAGATCATTTCAGTGGGACCTATT
AGGAGAAAAAGGCTGGACTGGTGTGAGGACAATGGAGGGTGTGAGCAGATTTGCACGAGC
CGGGTGGATGGGCCTCTCTGCAGCTGTGTAACAGGAACCCTGCAGGAGGACGGCAAGAGC
TGCAGAGCCTCTAATTCTTCAATGGAACTTCAAGTCTGGACGCTTCTTCTCATCATGATC
CAGATTTCATTATGGCATTTTGTCTATAAATCAGGCACGACCTCATTG
Protein sequence predicted based on nucleotide sequence
MNYSSFLRIWVSFIFALVQHQAQPRELMYPFWQNDTKTPKVDDGSSSEIKLAIPVFFFGV PYRTVYVNNNGVVSFNVLVSQFTPESFPLTDGRAFVAPFWADVHNGIRGEIYYRETMEPA ILKRATKDIRKYFKDMATFSATWVFIVTWEEVTFYGGSSTTPVNTFQAVLVSDGSYTFTL FNYYEINWTTGTASGGDPLTGLGGVMAQAGFNGGNLTNFFSLPGSRTPEIVNIQETTNVN VPGRWAFKVDGKEIDPANGCTSRGQFLRRGEVFWDDLNCTVKCRCLDFNNEIYCQEASCS PYEVCEPKGKFFYCSAVETSTCVVFGEPHYHTFDGFLFHFQGSCAYLLARQCLQTSSLPF FSVEAKNEHRRGSAVSWVKELSVEVNGYKILIPKGSYGRVKVNDLVTSLPVTLDLGTVKI YQSGISTAVETDFGLLVTFDGQHYASISVPGSYINSTCGLCGNYNKNPLDDFLRPDGRPA MSVLDLGESWRVYHADWKCDSGCVDNCTQCDAATEALYFGSDYCGFLNKTDGPLWECGTV VDPTAFVHSCVYDLCSVRDNGTLLCQAIQAYALVCQALGIPIGDWRTQTGCVSTVQCPSF SHYSVCTSSCPDTCSDLTASRNCATPCTEGCECNQGFVLSTSQCVPLHKCGCDFDGHYYT MGEFFWATANCTVQCLCEEGGDVYCFNKTCGSGEVCAVEDGYQGCFPKRETVCLLSQNQV LHTFDGASYAFPSEFSYTLLKTCPERPEYLEIDINKKKPDAGPAWLRGLRILVADQEVKI GGIGASEVKLNGQEVELPFFHPSGKLEIYRNKNSTTVESKGVVTVQYSDIGLLYIRLSTT YFNCTGGLCGFYNANASDEFCLPNGKCTDNLAVFLESWTTFEEICNGECGDLLKACNNDS ELLKFYRSRSRCGIINDPSNSSFLECHGVVNVTAYYRTCLFRLCQSGGNESELCDSVARY ASACKNADVEVGPWRTYDFCPLECPENSHFEECITCTETCETLTLGPICVDSCSEGCQCD EGYALLGSQCVTRSECGCNFEGHQLATNETFWVDLDCQIFCYCSGTDNRVHCETIPCKDD EYCMEEGGLYYCQARTDASCIVSGYGHYLTFDGFPFDFQTSCPLILCTTGSRPSSDSFPK FVVTAKNEDRDPSLALWVKQVDVTVFGYSIVIHRAYKHTVLVNSERLYLPLKLGQGKINI FSFGFHVVVETDFGLKVVYDWKTFLSITVPRSMQNSTYGLCGRYNGNPDDDLEMPMGLLA SSVNEFGQSWVKRDTFCQVGCGDRCPSCAKVEGFSKVQQLCSLIPNQNAAFSKCHSKVNP TFFYKNCLFDSCIDGGAVQTACSWLQNYASTCQTQGITVTGWRNYTSCTVTCPPNSHYES CVSVCQPRCAAIRLKSDCSHYCVEGCHCDAGYVLNGKSCILPHSCGCYSDGKYYEPKQLF WNSDCTRRCRCFRRNVIQCDPRQCKSDEECALRNGVRGCFSTKTSYCLAAGGGVFRTFDG AFLRFPANCAFVLSTICQKLPDISFQLIINFDKWSAPNLTIISPVYFYINEEQILINDRN TVKVNGTQVNVPFITGLATKIYSSEGFLVIDTSPDIQIYYNGFNVIKISISERLQNKVCG LCGNFNGDLTDDYVTLRGKPVVSSVVLAQSWKTNGMQKRPLAPSCNELQFSQYAAMCDNV HIQKMQGDGYCLKLTDMKGFFQPCYGLLDPLPFYESCYLDGCYSHKKFQLCGSLAAYGEA CRSFGILSTEWIEKENCSGVVEDPCVGADCPNRTCELGNGRELCGCIEPPPYGNNSHDII DAEVTCKAAQMEVSISKCKLFQLGFEREGVRINDRQCTGIEGEDFISFQINNTKGNCGNI VQSNGTHIMYKNTLWIESANNTGNIITRDRTINVEFSCAYELDIKISLDSVVKPMLSVIN LTVPTQEGSFITKMALYKNASYKHPYRQGEVVLTTRDVLYVGVFVVGADATHLILTLNKC YATPTRDSNDKLRYFIIEGGCQNLKDNTIGIEENAVSLTCRFHVTVFKFIGDYDEVHLHC AVSLCDSEKYSCKITCPHNSRIATDYTKEPKEQIISVGPIRRKRLDWCEDNGGCEQICTS RVDGPLCSCVTGTLQEDGKSCRASNSSMELQVWTLLLIMIQISLWHFVYKSGTTSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
