Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860407
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6330nts
Open reading frame: 1 to 6330
ATGGACGCGCTGGAGTCGTTGTTGGACGAAGTCGCTCTGGAGGGGCTCGATGGCCTGTGT
CTGCCAGCGCTGTGGAGCCGGCTGGAGACGCGAGTGCCGCCCTTCCCGCTGCCTTTGGAA
CCCTGCACGCAGGAGTTTCTCTGGCGGGCCCTCGCCACGCACCCGGGCATCAGCTTCTAT
GAGGAGCCTCGGGAGCGACCCGACCTACAGCTCCAGGACCGGTATGAAGAAATTGATTTG
GAAACTGGAATTTTGGAGTCTAGGAGGGACCCGGTGGCTTTGGAGGATGTCTACCCCATT
CATATGATCTTAGAGAATAAGGATGGCATCCAGGGCTCATGCCGCTACTTTAAGGAGAGG
AAAAACATTACCAATGACATCAGAACCAAGTCCTTGCAGCCTCGCTGTACAATGGTGGAA
GCCTTTGACAGGTGGGGGAAGAAACTGATCATCGTTGCCTCCCAGGCCATGCGGTACAGG
GCCTTGATAGGCCAGGAGGGGGATCCCGACCTGAAGCTGCCCGACTTCTCCTACTGCATC
CTGGAACGGCTAGGCCGGTCCAGGTGGCAAGGGGAGCTCCAGCGAGACCTTCACACCACT
GCTTTCAAGGTTGATGCTGGGAAGCTGCACTATCACAGAAAAATTTTGAACAAAAACGGG
CTGATTACAATGCAGTCCCATGTGATCCGATTACCCACTGGAGCCCAGCAACACTCAATC
CTCCTCCTACTGAACCGGTTTCATGTGGACAGGAGGAGCAAATACGACATCCTCATGGAG
AAGCTTTCGGTCATGCTGAGCACACGGACTAACCACATAGAGACGCTGGGAAAGCTGAGG
GAAGAGCTGGGGCTGTGCGAAAGGACGTTTAAGCGTCTGTACCAGTATATGCTGAACGCC
GGGCTAGCCAAGGTGGTGTCTCTTCGCTTGCAAGAGATCCACCCTGAATGTGGACCTTGT
AAGACAAAGAAAGGGACCGACGTCATGGTTCGGTGCCTCAAGCTGCTGAAGGAATTTAAA
CGGAATGACCATGATGATGACGAGGACGAGGAGGTCATCTCCAAGACAGTGCCTCCAGTG
GACATTGTGTTCGAGCGGGATATGCTCACACAGACCTACGACCTCATTGAGCGCAGAGGC
ACGAAAGGAATTTCCCAAGCTGAAATCCGAGTGGCTATGAATGTGGGAAAACTAGAAGCA
AGAATGCTGTGCCGACTTCTTCAAAGATTCAAAGTTGTCAAGGGATTCATGGAAGACGAA
GGTCGGCAGCGAACCACCAAGTACATTTCCTGCGTGTTTGCAGAGGAGAGCGACCTAAGC
CGGCAGTACCAAAGAGAGAAGGCCCGCAGCGAGCTCTTGACCACCGTGAGCCTGGCGTCT
ATGCAGGAGGAGTCGCTTCTGCCTGAAGGCGAGGACACCTTCCTCTCTGAGTCGGACAGT
GAGGAGGAGAGGAGCAGCAGCAAGCGGAGAGGCAGAGGGTCCCAGAAAGACACAAGAGCC
TCTGCAAACCTCCGGCCCAAGACCCAGCCTCATCACTCCACCCCAACCAAGGGTGGGTGG
AAAGTTGTAAACCTACACCCATTGAAAAAGCAGCCGCCCTCCTTCCCAGGAGCTGCTGAA
GAGAGAGCCTGCCAGAGCCTTGCCAGCAGGGACAGCCTCTTAGATACCAGCAGCGTCTCA
GAACCCAACGTGTCCTTTGTCTCCCACTGTGCGGACAGCAACAGTGGTGACATAGCTGTG
ATCGAGGAGGTCCGGATGGAAAACCCAAAGGAGAGTAGCAGTTCCCTGAAGACTGGGAGG
CACAGCTCAGGCCAAGACAAACCACACGAAACTTACCGACTGCTGAAACGCAGGAATCTG
ATCATAGAAGCTGTCACCAATCTTCGCTTAATCGAGAGTTTATTCACGATTCAGAAGATG
ATCATGGATCAGGAGAAGCAGGAAGGCGTGTCCACCAAGTGCTGCAAGAAGTCCATTGTC
CGCTTGGTGCGGAACCTGTCTGAGGAAGGTCTCTTGCGATTGTATCGGACCACTGTCATT
CAAGATGGCATCAAGAAGAAGGTGGATCTGGTGGTGCACCCGTCCATGGACCAGAACGAC
CCTCTAGTGAGAAGTGCCATCGAGCAGGTCCGCTTCCGGATCTCCAATTCAAGCACAGCC
AACAGGGTTAAAACTTCCCAGCCTCCAGTGCCCCAAGGGGAGGCAGAAGAAGACAGTCAA
GGAAAAGAGGGCCCAAGTGGATCAGGGGACTCTCAGCTGAGTGCTTCCTCTAGATCAGAA
AGTGGACGGATGAAAAAAAGTGATAATAAAATGGGCATAACCCCGCTTAGAAATTATCAC
CCCATTGTAGTTCCCGGACTGGGGCGTTCTCTAGGATTTCTGCCCAAAATGCCTCGCCTG
CGGGTGGTCCACATGTTTCTGTGGTACCTCATCTACGGGCACCCTGCCAGCAACACCGTG
GAGAAGCCAAGCTTCATCAGTGAACGGAGAACGATAAAGCAGGAGTCAGGCAGGGCAGGC
GTCCGGCCGTCCTCCTCTGGAAGTGCCTGGGAGGCCTGCTCTGAAGCCCCATCTAAAGGC
AGCCAAGATGGTGTCACCTGGGAGGCTGAAGTGGAGCTTGCCACGGAGACAGTGTATGTC
GACGATGCCTCGTGGATGCGCTACATCCCCCCAATCCCAGTCCACAGGGACTTCGGCTTT
GGCTGGGCTCTCGTCAGCGACATCCTCCTCTGCCTTCCCCTCTCCATCTTCATCCAGATT
GTGCAAGTCAGCTACAAGGTGGACAACCTGGAGGAATTTCTGAACGACCCGCTGAAGAAG
CACACGCTGATCCGCTTTCTCCCCAGGCCCATTCGGCAGCAGCTTCTGTACAAGAGGCGT
TACATTTTTTCGGTGGTGGAGAACCTTCAGAGGCTGTGCTACATGGGGCTGCTACAGTTT
GGTCCCACGGAAAAGTTTCAGGATAAAGATCAGGTCTTTATCTTCTTGAAGAAGAATGCA
GTCATTGTTGACACTACCATCTGCGACCCACATTACAACCTGGCCCGCAGCAGCCGGCCC
TTCGAGAGGCGCCTCTATGTCCTGAACTCAATGCAGGATGTGGAAAACTACTGGTTTGAC
CTGCAGTGCGTCTGCCTCAACACCCCACTAGGCGTGGTGCGCTGCCCGCGCGTCAGGAAG
AACAGCAGCACAGACCAGGGCAGCGACGAGGAGGGCAGCCTGCAGAAGGAGCAGGAGAGC
GCCATGGACAAGCACAACCTGGAGCGCAAGTGCGCCATGCTGGAGTACACCACTGGAAGC
CGTGAGGTGGTGGATGAAGGCTTGATCCCTGGAGATGGGCTGGGTGCCGCAGGGCTCGAT
TCCAGCTTCTACGGACACCTCAAGCGCAACTGGATCTGGACCAGCTACATCATCAACCAG
GCCAAAAAGGAGAACACTGCCGCAGAGAATGGACTCACAGTGAGGCTCCAGACATTTCTG
TCCAAGCGCCCAATGCCCCTCAGTGCCAGAGGCAACAGCAGGTTGAATATTTGGGGGGAA
GCAAGAGTAGGCTCCGAGCTCTGTGCTGGCTGGGAAGAGCAGTTTGAGGTGGACCGAGAG
CCCTCGCTGGACCGAAACCGGAGAGTGAGGGGTGGGAAAAGCCAGAAGCGGAAGCGGCTG
AAGAAGGACCCTGGGAAGAAGATCAAGAGAAAGAAGAAAGGAGAGTTCCCAGGAGAAAAA
AGCAAAAGGCTGCGCTACCATGATGAAGCCGACCAGAGTGCCCTGCAGCGGATGACGCGG
CTTCGTGTCACCTGGTCTATGCAGGAGGATGGGCTGCTTGTGCTGTGCCGCATTGCCAGC
AATGTCCTCAACACCAAGGTGAAGGGCCCATTTGTCACCTGGCAGGTGGTACGGGACATT
TTGCATGCCACGTTTGAAGAGTCTTTGGATAAAACATCTCATTCCGTTGGACGAAGAGCT
CGCTACATAGTCAAAAACCCACAGGCCTATCTCAACTATAAAGTGTGCCTGGCCGAGGTG
TACCAGGATAAAGCACTTGTTGGAGATTTCATGAATCGAAGAGGTGACTATGATGACCCA
AAGGTTTGTGCCAACGAGTTTAAAGAATTTGTGGAGAAGCTTAAAGAAAAGTTCAGTTCA
GCCCTAAGGAATTCTAACCTTGAAATCCCAGACACACTCCAGGAGCTGTTCGCCAGGTAC
CGAGTTTTGGCAATTGGGGATGAAAAAGATCAAACCAGGAAAGAGGATGAACTTAACAGC
GTGGATGACATCCACTTTCTGGTGCTTCAGAACCTGATCCAGAGCACGCTGGCCCTCTCA
GACAGTCAGATGAAGTCCTACCAGTCATTCCAGACTTTCCGCCTCTATCGGGAGTACAAG
GACCACGTTCTTGTGAAGGCCTTCATGGAGTGCCAGAAGAGGAGCTTGGTCAACCGGCGC
CGGGTCAACCACACGCTGGGCCCCAAGAAGAACCGGGCCCTCCCCTTCGTGCCAATGTCC
TACCAGCTATCCCAGACCTACTACAGGATTTTTACGTGGCGATTTCCAAGCACCATCTGC
ACGGAGTCATTCCAGTTTTTGGACAGAATGCGGGCTGCCGGCAAGTTGGACCAGCCTGAT
CGTTTCTCTTTCAAAGACCAGGATAATAACGAGCCCACAAACGACATGGTGGCCTTTTCA
CTGGACGGCCCTGGAGGAAATTGTGTGGCCGTCCTGACCCTCTTCTCTCTGGGCCTCATT
TCTGTGGATGTCAGGATCCCGGAGCAGATCATCGTGGTAGACAGCTCAATGGTGGAGAAT
GAGGTCATCAAAAGCTTGGGGAAGGACGGCAGCCTGGAGGATGACGAGGATGAAGAGGAT
GACTTGGACGAAGGTGTAGGGGGCAAGCGCCGGAGCATGGAGGTGAAACCTGCGCAAGCC
TCCCACACCAACTACCTGCTGATGAGGGGCTACTACTCCCCCGGCATCGTCAGCACCCGC
AACCTCAACCCCAACGACAGCATTGTGGTCAACTCCTGCCAGATGAAGTTCCAGCTCCGC
TGCACCCCTGTGCCCGCCCGGCTCAGGCCCGCTGCCGCTCCTCTGGAAGAGCTAACAATG
GGAACCTCCTGCCTCCCTGATACGTTCACCAAGCTGATAAACCCCCAGGAAAACACCTGC
AGCTTGGAGGAGTTTGTCCTCCAGCTGGAGCTGTCTGGGTATAGTCCCGAAGACCTGACT
GCTGCCTTGGAGATCTTGGAAGCCATTATAGCCACGGGTTGTTTTGGGATTGACAAGGAG
GAGCTGCGCAGACGGTTCTCGGCCTTGGAGAAGGCAGGTGGTGGGCGCACCAGGACATTC
GCAGATTGCATCCAGGCCCTCCTGGAGCAGCATCAGGTGCTGGAGGTCGGTGGCAACACT
GCGCGCCTGGTAGCCATGGGCTCTGCCTGGCCTTGGCTCCTGCACTCCGTGCGGCTGAAA
GACAGAGAAGACGCCGACATCCAGAGAGAAGACCCCCAGGCCAGACCCCTGGAGGGGTCT
TCCAGTGAGGACAGCCCCCCCGAGGGGCAGGCACCTCCTTCTCACAGCCCCCGGGGCACC
AAGAGGCGCGCCAGCTGGGCCAGTGAGAATGGGGAGACCGACGCCGAGGGCACCCAGATG
ACCCCTGCCAAGAGGCCAGCGCTCCAGGACTCAAATTTGGCCCCCAGCCTTGGGCCCGGA
GCTGAAGATGGGGCAGAAGCCCAGGCCCCATCTCCACCCCCAGCTCTTGAAGACACCGCT
GCAGCGGGAGCAGCACAGGAAGACCAAGAGGGTGTCGGTGAGTTCAGTTCCCCAGGCCAA
GAGCAGCTGAGCGGCCAGGCGCAGCCTCCAGAGGGCTCTGAAGACCCCAGAGGGTTCACA
GAGAGTTTCGGAGCTGCCAACATCTCCCAGGCAGCACGGGAAAGGGACTGTGAGAGTGTC
TGCTTCATCGGCCGGCCGTGGCGTGTCGTGGATGGCCACCTGAACCTTCCTGTATGCAAG
GGTATGATGGAGGCCATGCTGTACCACATCATGACCAGGCCTGGCATCCCCGAGAGCTCC
CTGCTGCGCCACTACCAGGGGGTCCTGCAGCCCGTCGCCGTGCTGGAGTTGCTCCAGGGC
CTGGAGTCCCTCGGCTGCATCCGGAAGCGCTGGCTGAGAAAGCCAAGGCCTGTCTCGCTC
TTCTCTACACCCGTGGTGGAAGAGGTGGAAGTGCCCTCCAGCCTGGACGAGAGCCCCATG
GCTTTCTATGAGCCCACCTTGGACTGTACCCTCCGGCTGGGCCGTGTGTTCCCCCACGAG
GTCAACTGGAACAAGTGGATCCACCTCTTG
Protein sequence predicted based on nucleotide sequence
MDALESLLDEVALEGLDGLCLPALWSRLETRVPPFPLPLEPCTQEFLWRALATHPGISFY EEPRERPDLQLQDRYEEIDLETGILESRRDPVALEDVYPIHMILENKDGIQGSCRYFKER KNITNDIRTKSLQPRCTMVEAFDRWGKKLIIVASQAMRYRALIGQEGDPDLKLPDFSYCI LERLGRSRWQGELQRDLHTTAFKVDAGKLHYHRKILNKNGLITMQSHVIRLPTGAQQHSI LLLLNRFHVDRRSKYDILMEKLSVMLSTRTNHIETLGKLREELGLCERTFKRLYQYMLNA GLAKVVSLRLQEIHPECGPCKTKKGTDVMVRCLKLLKEFKRNDHDDDEDEEVISKTVPPV DIVFERDMLTQTYDLIERRGTKGISQAEIRVAMNVGKLEARMLCRLLQRFKVVKGFMEDE GRQRTTKYISCVFAEESDLSRQYQREKARSELLTTVSLASMQEESLLPEGEDTFLSESDS EEERSSSKRRGRGSQKDTRASANLRPKTQPHHSTPTKGGWKVVNLHPLKKQPPSFPGAAE ERACQSLASRDSLLDTSSVSEPNVSFVSHCADSNSGDIAVIEEVRMENPKESSSSLKTGR HSSGQDKPHETYRLLKRRNLIIEAVTNLRLIESLFTIQKMIMDQEKQEGVSTKCCKKSIV RLVRNLSEEGLLRLYRTTVIQDGIKKKVDLVVHPSMDQNDPLVRSAIEQVRFRISNSSTA NRVKTSQPPVPQGEAEEDSQGKEGPSGSGDSQLSASSRSESGRMKKSDNKMGITPLRNYH PIVVPGLGRSLGFLPKMPRLRVVHMFLWYLIYGHPASNTVEKPSFISERRTIKQESGRAG VRPSSSGSAWEACSEAPSKGSQDGVTWEAEVELATETVYVDDASWMRYIPPIPVHRDFGF GWALVSDILLCLPLSIFIQIVQVSYKVDNLEEFLNDPLKKHTLIRFLPRPIRQQLLYKRR YIFSVVENLQRLCYMGLLQFGPTEKFQDKDQVFIFLKKNAVIVDTTICDPHYNLARSSRP FERRLYVLNSMQDVENYWFDLQCVCLNTPLGVVRCPRVRKNSSTDQGSDEEGSLQKEQES AMDKHNLERKCAMLEYTTGSREVVDEGLIPGDGLGAAGLDSSFYGHLKRNWIWTSYIINQ AKKENTAAENGLTVRLQTFLSKRPMPLSARGNSRLNIWGEARVGSELCAGWEEQFEVDRE PSLDRNRRVRGGKSQKRKRLKKDPGKKIKRKKKGEFPGEKSKRLRYHDEADQSALQRMTR LRVTWSMQEDGLLVLCRIASNVLNTKVKGPFVTWQVVRDILHATFEESLDKTSHSVGRRA RYIVKNPQAYLNYKVCLAEVYQDKALVGDFMNRRGDYDDPKVCANEFKEFVEKLKEKFSS ALRNSNLEIPDTLQELFARYRVLAIGDEKDQTRKEDELNSVDDIHFLVLQNLIQSTLALS DSQMKSYQSFQTFRLYREYKDHVLVKAFMECQKRSLVNRRRVNHTLGPKKNRALPFVPMS YQLSQTYYRIFTWRFPSTICTESFQFLDRMRAAGKLDQPDRFSFKDQDNNEPTNDMVAFS LDGPGGNCVAVLTLFSLGLISVDVRIPEQIIVVDSSMVENEVIKSLGKDGSLEDDEDEED DLDEGVGGKRRSMEVKPAQASHTNYLLMRGYYSPGIVSTRNLNPNDSIVVNSCQMKFQLR CTPVPARLRPAAAPLEELTMGTSCLPDTFTKLINPQENTCSLEEFVLQLELSGYSPEDLT AALEILEAIIATGCFGIDKEELRRRFSALEKAGGGRTRTFADCIQALLEQHQVLEVGGNT ARLVAMGSAWPWLLHSVRLKDREDADIQREDPQARPLEGSSSEDSPPEGQAPPSHSPRGT KRRASWASENGETDAEGTQMTPAKRPALQDSNLAPSLGPGAEDGAEAQAPSPPPALEDTA AAGAAQEDQEGVGEFSSPGQEQLSGQAQPPEGSEDPRGFTESFGAANISQAARERDCESV CFIGRPWRVVDGHLNLPVCKGMMEAMLYHIMTRPGIPESSLLRHYQGVLQPVAVLELLQG LESLGCIRKRWLRKPRPVSLFSTPVVEEVEVPSSLDESPMAFYEPTLDCTLRLGRVFPHE VNWNKWIHLL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
