Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860408
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3222nts
Open reading frame: 1 to 3222
ATGCACCAGCCGCCTGAGTCCACCGCCGCGGCCGCCGCCGCTGCAGACATTAGCGCTAGG
AAGATGGCGCACCCGGCAATGTTCCCTCGAAGGGGCAGCGGTAGTGGCAGCGCCTCTGCT
CTCAATGCAGCAGGTACCGGCGTCGGTAGTAATGCCACATCTTCCGAGGATTTTCCGCCT
CCGTCGCTGCTTCAGCCGCCGCCCCCTGCAGCATCTTCTACGTCGGGACCACAGCCTCCG
CCTCCACAAAGCCTGAACCTCCTTTCGCAGGCTCAGCTGCAGGCACAGCCTCTTGCGCCA
GGCGGAACTCAAATGAAAAAGAAAAGTGGCTTCCAGATAACTAGCGTTACTCCTGCTCAG
ATCTCCGCTAGTATCAGCTCTAACAACAGTATAGCAGAGGACACTGAGAGCTATGATGAT
CTGGATGAATCTCACACGGAAGATCTCTCTTCTTCGGAGATCCTTGATGTGTCACTTTCC
AGGGCTACTGACTTAGGGGAGCCCGAACGCAGCTCCTCAGAAGAGACCCTAAATAACTTC
CAGGAAGCCGAGACACCTGGGGCAGTCTCTCCCAACCAGCCCCACCTTCCTCAGCCTCAT
TTGCCTCACCTTCCACAACAGAATGTTGTGATCAATGGGAATGCTCATCCACACCACCTC
CATCACCACCATCAGATTCATCATGGGCACCACCTCCAACATGGTCACCACCATCCATCT
CATGTTGCTGTGGCCAGTGCATCCATTACTGGTGGGCCACCCTCAAGCCCAGTATCTAGA
AAACTCTCTACAACTGGAAGCTCTGACAGTATCACACCAGTTGCACCAACTTCTGCTGTA
TCATCCAGTGGTTCACCTGCATCTGTAATGACTAATATGCGTGCTCCAAGTACTACAGGT
GGAATAGGTATAAATTCTGTTACTGGCACTAGTACAGTAAATAATGTTAACATTACTGCT
GTGGGTAGTTTTAATCCTAATGTGACAAGCAGCATGCTTGGTAATGTTAATATAAGTACA
AGCAATATTCCTAGTGCTGCTGGTGTGAGTGTTGGGCCTGGAGTTACCAGTGGTGTTAAT
GTGAATATCTTGAGTGGCATGGGCAATGGTACTATTTCTTCCTCTGCTGCTGTTAGCAGT
GTTCCTAATGCAGCTGCAGGGATGACTGGGGGATCGGTTTCAAGTCAGCAGCAACAACCA
ACAGTTAACACTTCGAGGTTCAGAGTTGTGAAGTTAGATTCTAGTTCTGAGCCCTTTAAA
AAAGGTAGATGGACTTGCACTGAGTTCTATGAAAAAGAAAATGCTGTACCTGCTACAGAA
GGTGTGCTGATAAATAAAGTGGTGGAGACTGTAAAGCAAAATCCGATAGAAGTGACTTCT
GAAAGGGAGAGCACTAGTGGGAGTTCAGTGAGCAGTAGTGTCAGCACACTGAGTCACTAT
ACAGAGAGTGTGGGAAGTGGAGAGATGGGAGCCCCTACTGTGGTGGTGCAGCAGCAGCAG
CAGCAACAACAACAACAACAGCAACAACCAGCTCTCCAAGGTGTGACCCTCCAACAGATG
GATTTTGGTAGCACTGGTCCACAGAGTATTCCAGCAGTTAGTATACCACAGAGTATTTCT
CAGTCACAGATCTCACAAGTACAATTACAGTCTCAAGAACTGAGCTATCAGCAAAAGCAA
GGTCTTCAGCCAGTACCTCTGCAAGCCACTATGAGTGCTGCAACTGGTATCCAGCCATCG
CCTGTAAATGTGGTTGGTGTAACTTCAGCTTTAGGTCAGCAGCCTTCCATTTCCAGTTTG
GCTCAACCCCAGCTACCATATTCTCAGGCGGCTCCTCCAGTGCAAACTCCCCTTCCAGGG
GCACCACCACCCCAACAGTTACAGTATGGACAACAGCAACCAATGGTTTCTACACAGATG
GCCCCAGGCCATGTCAAATCAGTGACTCAAAATCCTGCTTCAGAGTATGTACAACAGCAG
CCAATTCTTCAAACAGCAATGTCCTCCGGACAGCCCAGTTCTGCAGGAGTAGGAGCAGGA
ACAACAGTGATTCCTGTGGCTCAGCCACAGGGTATCCAGCTGCCAGTGCAGCCCACAGCA
GTCCCAGCACAACCTGCAGGGGCATCTGTCCAGCCTGTTGGCCAGGCTCCGGCAGCAGTG
TCTGCTGTACCTACTGGCAGTCAGATTGCAAATATTGGTCAGCAAGCAAACATACCTACT
GCAGTGCAGCAGCCCTCTACCCAGGTTCCACCTTCAGTTATTCAGCAGGGTGCTCCTCCA
TCTTCGCAAGTGGTTCCACCTGCTCAAACTGGGATTATTCATCAGGGAGTTCAAACTAGT
GCTCCAAGCCTTCCTCAACAATTGGTTATTGCATCCCAAAGTTCCTTGTTAACTGTGCCT
CCCCAGCCACAAGGAGTAGAACCAGTAGCTCAAGGAATTGTTTCACAGCAGTTGCCTGCA
GTTAGTTCTTTGCCCTCTGCTAGTAGTATTTCTGTTACAAGTCAGGTTAGTTCAACTGGT
CCTTCTGGAATGCCTTCTGCCCCAACAAACTTGGTTCCACCACAAAATATAGCACAAACC
CCTGCTACCCAAAATGGTAATTTGGTTCAAAGTGTTAGTCAACCTCCCTTGATAGCAACT
AATACAAATTTGCCTTTGGCACAACAGATACCACTAAGTTCTACCCAGTTCTCCGCACAA
TCATTAGCTCAGGCAATTGGAAGCCAAATTGAAGATGCCAGGCGTGCAGCGGAGCCCTCC
TTAGTTGGCTTACCTCAGACTATCAGTGGTGACAGTGGGGGAATGTCAGCAGTTTCAGAT
GGGAGTAGCAGCAGCCTAGCAGCCTCTGCTTCTCTTTTCCCGTTGAAGGTGCTACCGCTG
ACGACACCCCTGGTGGATGGCGAGGATGAGAGCTCCTCTGGTGCAAGTGTGGTAGCTATT
GACAACAAAATCGAGCAAGCTATGGATCTAGTGAAAAGCCATTTGATGTATGCGGTCAGA
GAAGAAGTGGAGGTCCTCAAAGAGCAAATCAAAGAACTAATAGAGAAAAATTCCCAGCTG
GAGCAGGAGAACAATCTGCTGAAGACACTGGCCAGTCCTGAGCAGCTTGCCCAGTTTCAG
GCCCAGCTGCAGACTGGCTCCCCCCCTGCCACCACCCAGCCACAGGGCACCACACAGCCC
CCCGCCCAGCCAGCATCGCAGGGCTCAGGACCAACCGCATTG
Protein sequence predicted based on nucleotide sequence
MHQPPESTAAAAAAADISARKMAHPAMFPRRGSGSGSASALNAAGTGVGSNATSSEDFPP PSLLQPPPPAASSTSGPQPPPPQSLNLLSQAQLQAQPLAPGGTQMKKKSGFQITSVTPAQ ISASISSNNSIAEDTESYDDLDESHTEDLSSSEILDVSLSRATDLGEPERSSSEETLNNF QEAETPGAVSPNQPHLPQPHLPHLPQQNVVINGNAHPHHLHHHHQIHHGHHLQHGHHHPS HVAVASASITGGPPSSPVSRKLSTTGSSDSITPVAPTSAVSSSGSPASVMTNMRAPSTTG GIGINSVTGTSTVNNVNITAVGSFNPNVTSSMLGNVNISTSNIPSAAGVSVGPGVTSGVN VNILSGMGNGTISSSAAVSSVPNAAAGMTGGSVSSQQQQPTVNTSRFRVVKLDSSSEPFK KGRWTCTEFYEKENAVPATEGVLINKVVETVKQNPIEVTSERESTSGSSVSSSVSTLSHY TESVGSGEMGAPTVVVQQQQQQQQQQQQQPALQGVTLQQMDFGSTGPQSIPAVSIPQSIS QSQISQVQLQSQELSYQQKQGLQPVPLQATMSAATGIQPSPVNVVGVTSALGQQPSISSL AQPQLPYSQAAPPVQTPLPGAPPPQQLQYGQQQPMVSTQMAPGHVKSVTQNPASEYVQQQ PILQTAMSSGQPSSAGVGAGTTVIPVAQPQGIQLPVQPTAVPAQPAGASVQPVGQAPAAV SAVPTGSQIANIGQQANIPTAVQQPSTQVPPSVIQQGAPPSSQVVPPAQTGIIHQGVQTS APSLPQQLVIASQSSLLTVPPQPQGVEPVAQGIVSQQLPAVSSLPSASSISVTSQVSSTG PSGMPSAPTNLVPPQNIAQTPATQNGNLVQSVSQPPLIATNTNLPLAQQIPLSSTQFSAQ SLAQAIGSQIEDARRAAEPSLVGLPQTISGDSGGMSAVSDGSSSSLAASASLFPLKVLPL TTPLVDGEDESSSGASVVAIDNKIEQAMDLVKSHLMYAVREEVEVLKEQIKELIEKNSQL EQENNLLKTLASPEQLAQFQAQLQTGSPPATTQPQGTTQPPAQPASQGSGPTAL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
