Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860419
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6186nts
Open reading frame: 1 to 6186
ATGCTGCGAGTGATTGTGGAATCTGCCAGCAATATCCCTAAAACGAAATTTGGCAAGCCG
GATCCTATTGTTTCTGTCATTTTTAAGGATGAGAAAAAGAAAACAAAGAAAGTTGATAAT
GAATTGAACCCTGTCTGGAATGAGATTTTGGAGTTTGACTTGAGGGGTATACCACTGGAC
TTTTCATCTTCCCTTGGGATTATTGTGAAAGATTTTGAGACAATTGGACAAAATAAATTA
ATTGGCACGGCGACTGTAGCCCTGAAGGACCTGACTGGTGACCAGAGCAGATCCCTGCCG
TACAAGCTGATCTCCCTGCTAAATGAAAAAGGGCAAGATACTGGGGCCACCATTGACTTG
GTGATCGGCTATGATCCGCCTTCTGCTCCACATCCAAATGACCTGAGCGGGCCCAGCGTG
CCAGGCATGGGAGGAGATGGGGAAGAAGATGAAGGTGATGAAGACAGGTTGGACAATGCA
GTCAGGGGCCCTGGGCCCAAGGGGCCAGTTGGGACGGTGTCGGAAGCTCAGCTTGCTCGG
AGGCTCACCAAAGTAAAGAACAGCCGGCGGATGCTGTCAAATAAGCCACAGGACTTCCAG
ATCCGCGTCCGAGTGATTGAGGGCCGACAGTTAAGTGGTAACAACATAAGGCCTGTGGTC
AAAGTTCACGTCTGTGGCCAGACACACCGAACAAGAATCAAGAGAGGAAACAACCCTTTT
TTTGATGAGTTGTTTTTCTACAATGTCAACATGACCCCTTCTGAATTGATGGATGAGATC
ATCAGCATCCGGGTTTATAATTCTCACTCTCTGCGGGCAGATTGTCTGATGGGGGAATTT
AAGATTGATGTTGGATTTGTTTATGATGAACCTGGCCATGCTGTCATGAGAAAGTGGCTT
CTTCTCAATGACCCGGAAGATACCAGTTCAGGTTCTAAAGGTTATATGAAAGTCAGCATG
TTTGTCCTGGGAACCGGAGATGAGCCTCCTCCTGAGAGACGAGATCGTGATAATGACAGT
GATGATGTGGAGAGTAATTTGTTACTCCCTGCTGGCATTGCCCTCCGGTGGGTGACCTTC
TTGCTGAAAATCTACCGAGCTGAGGACATCCCCCAGATGGATGATGCCTTCTCACAGACA
GTAAAGGAAATATTTGGAGGCAATGCAGATAAGAAAAATCTCGTGGATCCTTTTGTAGAA
GTTTCCTTTGCTGGAAAAAAGGTTTGTACAAACATAATTGAGAAAAATGCAAACCCAGAG
TGGAATCAGGTCGTCAATCTTCAGATCAAGTTTCCTTCAGTGTGTGAAAAAATAAAACTA
ACAATATATGACTGGGACCGTCTTACTAAAAATGATGTAGTTGGAACAACATATCTACAC
CTCTCTAAAATTGCTGCCTCTGGTGGGGAAGTGGAAGATTTCTCATCTTCGGGAACTGGG
GCTGCATCATATACAGTAAACACAGGAGAAACAGAGGTAGGCTTTGTTCCAACGTTTGGA
CCTTGTTACCTGAATCTTTATGGAAGCCCCAGAGAGTACACGGGATTCCCAGACCCCTAT
GATGAGCTGAATACTGGAAAGGGGGAAGGAGTTGCCTACAGAGGCAGGATCTTGGTTGAA
TTAGCCACTTTTCTTGAGAAGACACCACCAGATAAAAAGCTTGAGCCCATTTCAAATGAT
GACCTGCTGGTTGTTGAGAAATACCAGCGAAGGCGGAAGTACAGCCTGTCTGCCGTGTTT
CATTCAGCCACCATGTTGCAAGATGTTGGTGAGGCCATTCAGTTTGAAGTCAGCATTGGG
AACTATGGCAACAAGTTTGACACCACCTGTAAGCCTTTGGCATCAACAACTCAGTACAGC
CGTGCTGTATTTGATGGCAACTACTATTATTACTTGCCTTGGGCCCACACCAAGCCAGTT
GTTACCCTGACTTCATACTGGGAGGATATTAGTCATCGCCTGGATGCGGTGAACACTCTC
CTAGCTATGGCAGAACGGCTGCAAACAAATATAGAAGCTCTAAAATCAGGGATACAAGGT
AAAATTCCTGCAAACCAGCTGGCTGAATTGTGGCTGAAGCTGATAGATGAAGTTATAGAA
GACACGAGATACACGTTGCCTCTCACAGAAGGAAAAGCCAACGTCACAGTTCTCGATACT
CAGATCCGAAAGCTGCGGTCCAGGTCTCTCTCCCAAATACATGAGGCGGCTGTGAGGATG
AGGTCGGAAGCCACAGATGTGAAGTCCACACTGGCAGAAATTGAGGACTGGCTTGATAAA
TTAATGCAGCTGACTGAAGAGCCACAGAACAGCATGCCTGACATCATCATCTGGATGATC
CGGGGAGAGAAGAGACTGGCCTATGCACGAATTCCCGCACATCAGGTCTTGTACTCCACC
AGTGGTGAGAATGCATCTGGAAAATACTGTGGGAAAACCCAAACCATCTTTCTGAAGTAT
CCACAGGAGAAAAACAACGGGCCAAAGGTGCCTGTGGAGTTGCGAGTGAACATCTGGCTA
GGCTTAAGTGCTGTGGAGAAGAAGTTTAACAGCTTCGCAGAAGGAACTTTCACCGTCTTT
GCTGAAATGTATGAAAATCAAGCTCTCATGTTTGGAAAATGGGGTACTTCTGGATTAGTA
GGACGTCATAAGTTTTCTGATGTCACAGGAAAAATAAAACTCAAGAGGGAATTTTTTCTG
CCTCCAAAAGGCTGGGAATGGGAAGGAGAGTGGATAGTTGATCCTGAAAGAAGCTTGCTG
ACTGAGGCAGATGCAGGTCACACGGAGTTCACTGATGAAGTCTATCAGAACGAGAGCCGC
TACCCCGGGGGCGACTGGAAGCCGGCCGAGGACACCTACACGGATGCGAACGGCGATAAA
GCAGCATCACCCAGCGAGTTGACTTGTCCTCCAGGTTGGGAATGGGAAGATGATGCATGG
TCTTATGACATAAATCGAGCGGTGGATGAGAAAGGCTGGGAATATGGAATCACCATTCCT
CCTGATCATAAGCCCAAATCCTGGGTTGCAGCAGAGAAAATGTACCACACTCATAGACGG
CGAAGGCTGGTCCGAAAACGCAAGAAAGATTTAACACAGACTGCTTCAAGCACCGCAAGG
GCCATGGAGGAATTGCAAGACCAAGAGGGCTGGGAATATGCTTCTCTAATTGGCTGGAAA
TTTCACTGGAAACAACGTAGTTCAGATACCTTCCGCCGCAGACGCTGGAGGAGAAAAATG
GCTCCTTCAGAAACACATGGTGCAGCTGCCATCTTTAAACTTGAAGGTGCCCTTGGGGCA
GACACTACCGAAGATGGGGATGAGAAGAGCCTGGAGAAACAGAAGCACAGTGCCACCACT
GTGTTCGGAGCAAACACCCCCATTGTTTCCTGCAATTTTGACAGAGTCTACATCTACCAT
CTGCGCTGCTATGTCTATCAAGCCAGAAACCTCTTGGCTTTAGATAAGGATAGCTTTTCA
GATCCATATGCTCATATCTGTTTCCTCCATCGGAGCAAAACCACTGAGATCATCCATTCA
ACCCTGAATCCCACGTGGGACCAAACAATTATATTCGATGAAGTTGAAATCTATGGGGAA
CCCCAAACAGTTCTACAGAATCCACCCAAAGTTATCATGGAACTTTTTGACAATGACCAA
GTGGGCAAAGATGAATTTTTAGGACGAAGCATTTTCTCTCCTGTGGTGAAACTGAACTCA
GAAATGGACATCACACCCAAACTTCTCTGGCACCCAGTAATGAATGGAGACAAAGCCTGC
GGGGATGTTCTTGTAACTGCAGAGCTGATTCTGAGGGGCAAGGATGGCTCCAACCTTCCC
ATTCTTCCCCCTCAAAGGGCGCCAAATCTATACATGGTCCCCCAGGGGATCAGGCCTGTG
GTCCAGCTCACTGCCATTGAGATTCTAGCTTGGGGCTTAAGAAATATGAAAAACTTCCAG
ATGGCTTCTATCACATCCCCCAGTCTTGTTGTGGAGTGTGGAGGAGAAAGGGTGGAATCG
GTGGTGATCAAAAACCTTAAGAAGACACCCAACTTTCCAAGTTCTGTTCTCTTCATGAAA
GTGTTCTTGCCCAAGGAGGAATTGTACATGCCCCCACTGGTGATCAAGGTCATCGACCAC
AGGCAGTTTGGGCGGAAGCCTGTCGTCGGCCAGTGCACCATCGAGCGCCTGGACCGCTTT
CGCTGTGACCCTTATGCAGGGAAAGAGGACATCGTCCCACAGCTCAAAGCCTCCCTTCTG
TCTGCCCCACCATGCCGGGACATCGTTATCGAAATGGAAGACACCAAACCATTACTGGCT
TCTAAGCTGACAGAAAAGGAGGAAGAAATCGTGGACTGGTGGAGTAAATTTTATGCTTCC
TCAGGGGAACATGAAAAATGCGGACAGTATATTCAGAAAGGCTATTCCAAGCTCAAGATA
TATAATTGTGAACTAGAAAATGTAGCAGAATTTGAGGGCCTGACAGACTTCTCAGATACG
TTCAAGTTGTACCGAGGCAAGTCGGATGAAAATGAAGATCCTTCTGTGGTTGGAGAGTTT
AAGGGCTCCTTTCGGATCTACCCTCTGCCGGATGACCCCAGCGTGCCAGCCCCTCCCAGA
CAGTTTCGGGAATTACCTGACAGCGTCCCACAGGAATGCACGGTTAGGATTTACATTGTT
CGAGGCTTAGAGCTCCAGCCCCAGGACAACAATGGCCTGTGTGACCCTTACATAAAAATA
ACACTGGGCAAAAAAGTCATTGAAGACCGAGATCACTACATTCCCAACACTCTCAACCCA
GTCTTTGGCAGGATGTACGAACTGAGCTGCTACTTACCTCAAGAAAAAGACCTGAAAATT
TCTGTCTATGATTATGACACCTTTACCCGGGATGAAAAAGTAGGAGAAACAATTATTGAT
CTGGAAAACCGATTCCTTTCCCGCTTTGGGTCCCACTGCGGCATACCAGAGGAGTACTGT
GTTTCTGGAGTCAATACCTGGCGAGATCAACTGAGACCAACACAGCTGCTTCAAAATGTC
GCCAGATTCAAAGGCTTCCCACAACCCATCCTTTCCGAAGATGGGAGTAGAATCAGATAT
GGAGGACGAGACTACAGCTTGGATGAATTTGAAGCCAACAAAATCCTGCACCAGCACCTC
GGGGCCCCTGAAGAGCGGCTTGCTCTTCACATCCTCAGGACTCAGGGGCTGGTCCCTGAG
CACGTGGAAACAAGGACTTTGCACAGCACCTTCCAGCCCAACATTTCCCAGGGAAAACTT
CAGATGTGGGTGGATGTTTTCCCCAAGAGTTTGGGGCCACCAGGCCCTCCTTTCAACATC
ACACCCCGGAAAGCCAAGAAATACTACCTGCGTGTGATCATCTGGAACACCAAGGACGTT
ATCTTGGACGAGAAAAGCATCACAGGAGAGGAAATGAGTGACATCTACGTCAAAGGCTGG
ATTCCTGGCAATGAAGAAAACAAACAGAAAACAGATGTCCATTACAGATCTTTGGATGGT
GAAGGGAATTTTAACTGGCGATTTGTTTTCCCGTTTGACTACCTTCCAGCCGAACAACTC
TGTATCGTTGCGAAAAAAGAGCATTTCTGGAGTATTGACCAAACGGAATTTCGAATCCCA
CCCAGGCTGATCATTCAGATATGGGACAATGACAAGTTTTCTCTGGATGACTACTTGGGT
TTCCTAGAACTTGACTTGCGTCACACGATCATTCCTGCAAAATCACCAGAGAAATGCAGA
TTGGACATGATTCCGGACCTCAAAGCCATGAACCCCCTTAAAGCCAAGACAGCCTCCCTC
TTTGAGCAGAAGTCCATGAAAGGATGGTGGCCATGCTACGCAGAGAAAGATGGCGCCCGC
GTAATGGCTGGGAAAGTGGAGATGACATTGGAAATCCTCAACGAGAAGGAGGCCGACGAG
AGGCCAGCCGGGAAGGGGCGGGACGAACCCAACATGAACCCCAAGCTGGACTTACCAAAT
CGACCAGAAACCTCCTTCCTCTGGTTCACCAACCCATGCAAGACCATGAAGTTCATCGTG
TGGCGCCGCTTTAAGTGGGTCATCATCGGCTTGCTGTTCCTGCTTATCCTGCTGCTCTTC
GTGGCCGTGCTCCTCTACTCTTTGCCGAACTATTTGTCAATGAAGATTGTAAAGCCAAAT
GTGTTG
Protein sequence predicted based on nucleotide sequence
MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLD FSSSLGIIVKDFETIGQNKLIGTATVALKDLTGDQSRSLPYKLISLLNEKGQDTGATIDL VIGYDPPSAPHPNDLSGPSVPGMGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLAR RLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPF FDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWL LLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTF LLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPE WNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGTG AASYTVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVE LATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIG NYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTL LAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDT QIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMI RGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWL GLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFL PPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDK AASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRR RRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKM APSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYH LRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGE PQTVLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKAC GDVLVTAELILRGKDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQ MASITSPSLVVECGGERVESVVIKNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDH RQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVPQLKASLLSAPPCRDIVIEMEDTKPLLA SKLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNCELENVAEFEGLTDFSDT FKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIV RGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKI SVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNV ARFKGFPQPILSEDGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPE HVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDV ILDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQL CIVAKKEHFWSIDQTEFRIPPRLIIQIWDNDKFSLDDYLGFLELDLRHTIIPAKSPEKCR LDMIPDLKAMNPLKAKTASLFEQKSMKGWWPCYAEKDGARVMAGKVEMTLEILNEKEADE RPAGKGRDEPNMNPKLDLPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLFLLILLLF VAVLLYSLPNYLSMKIVKPNVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
