Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860421
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5349nts
Open reading frame: 1 to 5349
ATGAGCCGTGGCTTTTGGCTGGCGGAGCCGCTGGCAGGGACCGGGCCCCACCCTGCCCCA
GTGGCTGCCGACTCACGCGGGTGCAGCTCTGTTCCGCGGAGGCACGCCCCCTCCCGTCTC
TCCGTCTCGACCCCCTCGCGGGGCCCAGGTGCCAGGATGGTGGGAGAACTCCGCTACAGG
GAATTCAGGGTGCCCCTGGGGCCCGGCTTACATGCCTATCCTGATGAGCTGATCCGCCAG
CGCGTGGGCCATGATGGGCATCCTGAGTACCAGATCCGTTGGCTCATCCTGCGGCGTGGC
GATGAGGGGGACGGGGGCTCTGGCCAAGTGGACTGCAAGGCTGAGCACATCCTGCTGTGG
ATGTCCAAGGATGAGATCTATGCCAACTGCCACAAGATGCTGGGCGAGGATGGCCAGGTC
ATCGGGCCCTCCCAGGAGTCTGCAGGGGAGGTTGGGGCCCTGGACAAATCTGTGCTGGAG
GAGATGGAAACCGACGTGAAGTCCCTCATTCAGAGAGCCCTTCGGCAGCTGGAGGAGTGT
GTGGGCACTATCCCTCCTGCTCCTCTACTTCACACTGTCCACGTGCTCAGCGCCTATGCC
AGCATTGAGCCCCTCACTGGAGTATTCAAGGACCCAAGGGTCCTGGACTTGCTCATGCAC
ATGTTGAGTAGTCCTGATTATCAGATTCGCTGGAGTGCAGGCCGGATGATACAAGCCCTG
TCCTCCCATGACGCTGGTGAGGGGCAGTGTGGGGAGGAAGGGAAAGCAGGAGAAGGGCTG
GGTCGGCTCAGGGACTCACAGGACACTGTGGCAGGAGCCTCTGATCTCATCAGGACCCGG
ACTCAGATCCTTCTGTCACTGAGCCAACAAGAAGCCATTGAGAAACACCTGGATTTTGAC
AGCCGCTGTGCTCTGCTAGCACTGTTTGCACAGGCCACGCTCTCTGAACACCCCATGTCT
TTCGAGGGCATTCAGCTACCACAGGTCCCAGGAAGGGTGCTCTTCTCCCTGGTGAAGCGG
TATTTGCATGTCACCTCGCTCCTGGATCAGCTGAACGACAGTGCTGCGGAGCCAGGAGCC
CAGAACACCTCTGCTCCTGAGGAGTTGAGTGGGGAGAGGGGTCAACTGGAGCTGGAGTTC
AGTATGGCCATGGGCACCCTGATCTCGGAGCTGGTGCAAGCCATGCGCTGGGACCAGGCC
TCAGACAGACCAAGGAGCTCAGCACGGTCCCCCGGTTCCATCTTCCAGCCTCAGCTGGCA
GATGTGAGCCCAGGGCTCCCCGCTGCCCAGGCTCAGCCCTCCTTCAGGAGGTCAAGACGT
TTTCGCCCTCGTTCTGAGTTCGCAAGTGGCAATACCTATGCTTTGTATGTGCGGGACACA
CTGCAGCCGGGGATGCGAGTGCGGATGCTGGATGATTATGAGGAGATCAGTGCCGGGGAT
GAGGGCGAGTTTCGGCAGAGCAACAACGGTGTGCCTCCTGTGCAGGTATTTTGGGAGTCA
ACAGGCCGCACCTATTGGGTGCACTGGCACATGCTGGAGATCTTGGGCTTTGAGGAAGAC
ATTGAGGACATGGTTGAGGCTGATGAGTACCAAGGGGCAGTGGCCAGTAGAGTCCTGGGT
AGAGCCCTGCCTGCCTGGCGCTGGAGGCCCATGACAGAACTCTATGCTGTGCCTTATGTG
CTGCCTGAGGATGAGGACACTGAGGAGTGTGAACACCTGACCCTGGCTGAGTGGTGGGAA
CTCCTCTTCTTCATCAAGAAGCTGGATGGACCTGACCATCAGGAGGTTCTCCAGATCCTC
CAGGAGAACCTAGATGGGGAGATTCTGGATGATGAGATCCTAGCTGAACTGGCCGTGCCC
ATAGAATTGGCCCAGGACTTGCTGCTGACTCTGCCACAGCGACTCAATGACAGTGCCCTC
AGGGACCTGATCAACTGCCATGTCTACAAGAAGTATGGGCCTGAAGCCCTAGCAGGGAAC
CAAGCCTACCCATCCCTTCTAGAAGCCCAAGAAGATGTCCTCCTGCTAGACGCGCAGGCC
CAGGCTAAGGACTCAGAAGATGCAGCCAAAGTGGAAGCAAAAGAACCCCCATCTCAGAGT
CCCAACACTCCCCTGCAGCGTCTGGTGGAGGGTTATGGTCCAGCTGGGAAAATCCTCCTG
GATCTAGAGCAAGCCCTCAGCTCAGAGGGGACCCAGGAGAACAAGGTCAAGCCACTCCTG
CTGCAGCTGCAGCGGCAGCCGCAGCCCTTCCTGGCACTGATGCAGAGCCTGGACACTCCG
GAGACTAACAGGACCCTGCACCTGACTGTGCTGAGAATCCTGAAGCAGCTGGTGGACTTC
CCCGAGGCACTGCTGCTCCCCTGGCACGAGGCCGTGGATGCCTGCATGGCCTGCCTGCGG
TCCCCAAACACTGATCGAGAGGTGCTCCAGGAACTGATTTTCTTCCTGCACCGCCTGACC
TCAGTGAGCAGGGACTATGCCGTGGTGCTGAATCAGCTGGGAGCAAGAGACGCTATCTCC
AAGGCCCTGGAAAAGCACCTGGGAAAGCTGGAGCTGGCTCAGGAGCTGCGGGACATGGTG
TTCAAGTGTGAGAAGCATGCCCACCTCTACCGCAAACTCATCACCAACATCCTGGGAGGC
TGCATCCAGATGGTGCTGGGCCAGATCGAAGACCACAGACGAACCCACCAACCCATCAAC
ATCCCTTTCTTTGATGTGTTCCTCAGATACCTGTGCCAGGGCTCCAGTGTGGAAGTGAAG
GAGGACAAGTGCTGGGAGAAGGTGGAGGTGTCCTCCAACCCGCACCGGGCCAGCAAGCTG
ACGGACCACAACCCCAAGACCTATTGGGAGTCCAACGGCAGCGCCGGCTCCCACTACATC
ACCCTGCACATGCGCCGGGGCATCCTCATCAGGCAACTGACTCTGCTTGTGGCTAGTGAG
GACTCGAGTTACATGCCGGCCCGAGTGGTGGTGTGCGGGGGTGATAGCACTAGCTCTCTT
CACACGGAACTCAACTCGGTGAATGTGATGCCCTCTGCCAGCCGGGTGATCCTCCTGGAG
AACCTGACCCGCTTCTGGCCCATCATCCAGATCCGCATAAAGCGCTGCCAGCAGGGTGGC
ATTGATACGCGCATTCGGGGGTTAGAGATCCTAGGCCCCAAGCCCACGTTCTGGCCAGTG
TTCCGGGAGCAGCTCTGTCGTCACACACGCCTCTTCTACATGGTTCGGGCACAGGCCTGG
AGCCAGGACATGGCAGAGGACCGCAGGAGCCTCCTGCACCTGAGTTCTAGACTCAACGGT
GCTCTGCGCCAGGAGCAGAATTTTGCTGACCGCTTCCTCCCTGATGACGAGGCTGCCCAA
GCTCTGGGCAAGACCTGCTGGGAGGCCCTGGTCAGCCCCGTGGTGCAGAACATCACCTCC
CCTGATGAGGATGGCATTAGCCCCCTGGGTTGGCTGCTGGACCAGTACCTGGAGTGTCAG
GAAGCTGTCTTCAACCCCCAGAGCCGCGGCCCAGCTTTCTTCTCGCGGGTGCGCCGTCTC
ACTCACCTGCTGGTGCATGTCGAGCCCTGTGAGGCACCCCCTCCTGTGGTGGCCACTCCT
CGGCCCAAAGGCAGAAACAGAAGCCACGACTGGAGCTCCTTGGCTACCCGGGGCCTTCCA
AGCAGCATCATGAGAAACCTGACGCGCTGTTGGCGGGCCGTGGTGGAGAAGCAGGTGAAC
AATTTTCTGACCTCATCCTGGCGGGATGATGACTTTGTGCCACGCTACTGTGAGCACTTT
AATATTCTGCAGAACTCAAGCTCTGAACTGTTTGGGCCTCGGGCAGCCTTCTTGCTGGCG
CTGCAAAATGGCTGTGCGGGAGCCTTGCTGAAGCTCCCTTTTCTCAAAGCTGCCCACGTG
AGTGAGCAGTTCGCCCGGCACATTGACCAGCAGATCCAGGGCAGCCGGATCGGTGGAGCC
CAGGAAATGGAGAGGCTGGCACAGCTGCAGCAATGCCTGCAAGCTGTCCTGATTTTCTCC
GGCTTGGAGATAGCCACCACTTTTGAGCATTATTACCAGCACTACATGGCGGACCGTCTC
CTGGGCGTGGTCTCGAGCTGGCTGGAGGGGGCCGTGCTGGAGCAGATCGGTCCCTGCTTC
CCCAACCGCCTCCCCCAGCAGATGTTGCAGAGCCTGAGCACCTCTAAGGAGCTGCAGCGC
CAGTTCCACGTCTACCAGCTCCAGCAGCTGGATCAGGAACTCCTGAAGCTGGAGGATACA
GAGAAGAAAATACAGGTGGGCCTTGGGGCCAGTGGCAAGGAGCACAAGAGCGAGAAGGAA
GAGGAAGCTGGGGCAGCAGCAGTGGTGGATGTGGCGGAGGGAGAGGAGGAAGAGGAGGAG
AATGAGGACCTCTACTATGAAGGGGCAATGCCAGAAGTGTCTGTGCTTGTCCTGTCCCGA
CACTCCTGGCCTGTTGCCTCAATCTGCCACACACTGAACCCCAGAACCTGCCTGCCCTCC
TACCTGAGGGGCACTTTGAACAGATACTCCAACTTCTACAACAAGAGTCAGAGCCACCCT
GCCCTTGAGCGAGGCTCACAGAGGCGACTGCAGTGGACGTGGCTGGGCTGGGCTGAGCTG
CAGTTTGGGAACCAGACCCTGCATGTGTCCACCGTGCAGATGTGGCTACTGCTGTATCTC
AACGACCTGAAGGCGGTCTCTGTGGAGAGTCTGCTGGCGTTCTCAGGGCTCTCCGCAGAC
ATGCTCAATCAGGCGATTGGGCCCCTCACCTCTTCAAGAGGCCCCCTGGACCTTCACGAG
CAAAAGGATATACCAGGAGGGGTCCTCAAGATTCGAGATGGCAGCAAGGAACCCAGGTCG
AGATGGGACATTGTGCGGCTCATCCCACCTCAGACGTACCTGCAAGCTGAGGGTGAAGAC
GGCCAGAACTTGGAGAAGAGACGGAATCTTCTGAACTGCCTCATCGTCCGAATCCTCAAG
GCCCATGGAGATGAGGGGCTGCACATTGACCAGCTTGTCTGTCTGGTGCTGGAGGCTTGG
CAGAAGGGCCCGTGTCCTCCCAGGGGTTTGGTCAGCAGCCTTGGTAAGGGGTCTGCATGC
AGCAGCACTGACGTCCTCTCCTGCATCCTACACCTCCTGGGCAAGGGCACGCTGAGACGC
CATGACGACCGGCCCCAGGTGCTGTCCTATGCAGTCCCTGTGACTGTCATGGAGCCTCAC
ACTGAGTCCCTGAACCCAGGCTCCTCAGGCCCCAACCCACCCCTCACCTTCCATACCCTA
CAGATTCGCTCCCGGGGTGTGCCCTATGCCTCCTGCACTGCCACCCAGAGCTTCTCTACC
TTCCGGTTG
Protein sequence predicted based on nucleotide sequence
MSRGFWLAEPLAGTGPHPAPVAADSRGCSSVPRRHAPSRLSVSTPSRGPGARMVGELRYR EFRVPLGPGLHAYPDELIRQRVGHDGHPEYQIRWLILRRGDEGDGGSGQVDCKAEHILLW MSKDEIYANCHKMLGEDGQVIGPSQESAGEVGALDKSVLEEMETDVKSLIQRALRQLEEC VGTIPPAPLLHTVHVLSAYASIEPLTGVFKDPRVLDLLMHMLSSPDYQIRWSAGRMIQAL SSHDAGEGQCGEEGKAGEGLGRLRDSQDTVAGASDLIRTRTQILLSLSQQEAIEKHLDFD SRCALLALFAQATLSEHPMSFEGIQLPQVPGRVLFSLVKRYLHVTSLLDQLNDSAAEPGA QNTSAPEELSGERGQLELEFSMAMGTLISELVQAMRWDQASDRPRSSARSPGSIFQPQLA DVSPGLPAAQAQPSFRRSRRFRPRSEFASGNTYALYVRDTLQPGMRVRMLDDYEEISAGD EGEFRQSNNGVPPVQVFWESTGRTYWVHWHMLEILGFEEDIEDMVEADEYQGAVASRVLG RALPAWRWRPMTELYAVPYVLPEDEDTEECEHLTLAEWWELLFFIKKLDGPDHQEVLQIL QENLDGEILDDEILAELAVPIELAQDLLLTLPQRLNDSALRDLINCHVYKKYGPEALAGN QAYPSLLEAQEDVLLLDAQAQAKDSEDAAKVEAKEPPSQSPNTPLQRLVEGYGPAGKILL DLEQALSSEGTQENKVKPLLLQLQRQPQPFLALMQSLDTPETNRTLHLTVLRILKQLVDF PEALLLPWHEAVDACMACLRSPNTDREVLQELIFFLHRLTSVSRDYAVVLNQLGARDAIS KALEKHLGKLELAQELRDMVFKCEKHAHLYRKLITNILGGCIQMVLGQIEDHRRTHQPIN IPFFDVFLRYLCQGSSVEVKEDKCWEKVEVSSNPHRASKLTDHNPKTYWESNGSAGSHYI TLHMRRGILIRQLTLLVASEDSSYMPARVVVCGGDSTSSLHTELNSVNVMPSASRVILLE NLTRFWPIIQIRIKRCQQGGIDTRIRGLEILGPKPTFWPVFREQLCRHTRLFYMVRAQAW SQDMAEDRRSLLHLSSRLNGALRQEQNFADRFLPDDEAAQALGKTCWEALVSPVVQNITS PDEDGISPLGWLLDQYLECQEAVFNPQSRGPAFFSRVRRLTHLLVHVEPCEAPPPVVATP RPKGRNRSHDWSSLATRGLPSSIMRNLTRCWRAVVEKQVNNFLTSSWRDDDFVPRYCEHF NILQNSSSELFGPRAAFLLALQNGCAGALLKLPFLKAAHVSEQFARHIDQQIQGSRIGGA QEMERLAQLQQCLQAVLIFSGLEIATTFEHYYQHYMADRLLGVVSSWLEGAVLEQIGPCF PNRLPQQMLQSLSTSKELQRQFHVYQLQQLDQELLKLEDTEKKIQVGLGASGKEHKSEKE EEAGAAAVVDVAEGEEEEEENEDLYYEGAMPEVSVLVLSRHSWPVASICHTLNPRTCLPS YLRGTLNRYSNFYNKSQSHPALERGSQRRLQWTWLGWAELQFGNQTLHVSTVQMWLLLYL NDLKAVSVESLLAFSGLSADMLNQAIGPLTSSRGPLDLHEQKDIPGGVLKIRDGSKEPRS RWDIVRLIPPQTYLQAEGEDGQNLEKRRNLLNCLIVRILKAHGDEGLHIDQLVCLVLEAW QKGPCPPRGLVSSLGKGSACSSTDVLSCILHLLGKGTLRRHDDRPQVLSYAVPVTVMEPH TESLNPGSSGPNPPLTFHTLQIRSRGVPYASCTATQSFSTFRL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
