Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860428
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 7029nts
Open reading frame: 1 to 7029
ATGGATGGGGTGTCTTCAGAGGCTAATGAAGAAAATGACAATATAGAGAGACCTGTTAGA
AGACGGCATTCTTCAATATTGAAACCCCCAAGGAGTCCTCTTCAGGACCTCAGAGGTGGG
AATGAAAGAGTTCAGGAATCCAATGCTTTGAGAAATAAGAAAAACTCTCGTCGAGTCAGC
TTTGCAGATACTATAAAGGTATTCCAGACGGAGTCTCATATGAAAATAGTGAGAAAGTCA
GAAATGGAAGGCTGCAGTGCAATGGTGCCATCTCAGCTGCAACTTCTGCCTCCTGGGTTT
AAGCGATTCTCCTGCCTCAGCCTCCCAGAAACAGAAACAGGAGAAAATCTTCTTTTGATA
CAGAATAAGAAATTAGAAGATAATTACTGTGAAATTACTGGGATGAACACATTGCTTTCT
GCTCCCATTCATACCCAGATGCAACAGAAGGAGTTTTCAATTATAGAACATACCCGTGAA
AGGAAACATGCAAATGACCAGACAGTCATTTTTTCAGATGAAAACCAGATGGACCTGACA
TCAAGTCACACTGTAATGATTACCAAAGGCCTTTTAGATAATCCCATAAGTGAAAAGTCC
ACCAAGATAGATACCACATCATTTCTAGCTAATTTAAAGCTTCACACCGAGGACTCAAGA
ATGAAAAAAGAAGTAAATTTTTCCGTGGATCAAAACACTTCTTCAGAAAATAAAATAGAT
TTCAATGACTTCATAAAAAGATTGAAAACAGGAAAATGTAGTGCTTTTCCTGATGTGCCT
GATAAAGAAAATTTTGAGATACCTATTTATTCCAAGGAACCGAACAGTGCCTCTTCTACA
CATCAAATGCATGTATCTCTTAAGGAAGATGAAAATAACAGTAATATTACTAGGCTCTTT
AGAGAAAAAGATGATGGGATGAATTTCACCCAGTGTCATACAGCCAATATTCAGACATTG
ATTCCCACATCCAGTGAGACCAACTCACGGGAATCTAAAGGTAATGATATTACAATTTAT
GGCAATGACTTTATGGACTTGACATTTAACCACACTTTGCAGATCTTACCTGCAACAGGT
AATTTTTCTGAAATAGAAAATCAAACTCAGAATGCCATGGATGTAACAACAGGTTATGGA
ACTAAAGCTTCAGGAAATAAAACAGTTTTTAAGAGTAAACAAAATACTGCTTTTCAAGAC
CTTTCCATAAACTCTGCAGACAAAATACATATTACCAGAAGTCATATTATGGGGGCAGAA
ACTCACATAGTCTCACAGACTTGTAATCAGGATGCCAGAATATTAGCCATGACCCCAGAA
TCTATATATTCTAATCCATCTATTCAAGGTTGTAAGACTGTTTTCTATTCTAGTTGTAAT
GATGCCATGGAAATGACCAAATGTCTCTCAAATATGAGAGAGGAGAAAAATTTGCTAAAG
CATGACAGTAATTATGCTAAAATGTATTGCAATCCAGATGCTATGTCTTCTCTCACAGAG
AAAACTATTTATTCCGGAGAGGAGAACATGGACATTACCAAGAGTCATACAGTTGCAATA
GATAATCAAATTTTTAAACAAGATCAATCAAATGTGCAAATAGCAGCTGCACCAACACCC
GAAAAAGAAATGATGCTCCAAAATCTTATGACCACATCAGAAGATGGGAAAATGAATGTA
AATTGTAACTCAGTTCCTCATGTATCTAAGGAAAGAATACAGCAGAGCCTGTCAAATCCT
TTGTCTATTTCATTGACTGATAGAAAGACTGAACTCTTATCAGGTGAAAATATGGATTTG
ACTGAAAGTCACACAAGTAACTTAGGAAGTCAGGTTCCTCTTGCAGCTTATAATCTAGCA
CCGGAGAGTACCAGTGAATCTCACTCTCAGAGCAAAAGCTCTTCAGATGAATGTGAAGAA
ATTACCAAAAGTCGTAATGAACCATTTCAGCGATCAGACATAATAGCCAAAAACAGCTTA
ACCGACACCTGGAACAAAGACAAAGATTGGGTTTTGAAGATTTTGCCCTACCTTGATAAA
GATTCTCCTCAGTCAGCTGATTGTAATCAGGAGATAGCAACAAGCCATAATATAGTCTAC
TGTGGTGGAGTTCTTGATAAACAAATAACTAATAGAAATACAGTATCATGGGAACAATCT
TTGTTTTCTACCACAAAGCCATTATTTTCATCAGGACAGTTCTCTATGAAAAATCATGAT
ACTGCTATAAGTAGTCATACAGTGAAATCTGTACTAGGCCAGAATTCTAAACTGGCTGAG
CCACTGAGGAAAAGTTTAAGCAATCCCACACCTGACTATTGCCATGACAAGATGATTATA
TGTTCAGAGGAAGAGCAAAATATGGATCTAACAAAGAGCCACACTGTCGTCATTGGATTT
GGTCCTTCTGAACTACAAGAACTTGGTAAAACTAATTTAGAACACACTACTGGCCAGCTA
ACAACAATGAACAGACAGATAGCTGTAAAAGTTGAAAAATGTGGTAAAAGTCCCATAGAA
AAAAGTGGAGTGCTTAAATCTAACTGTATTATGGATGTGTTAGAGGACGAAAGTGTACAG
AAACCTAAATTTCCAAAGGAAAAGCAAAATGTCAAAATTTGGGGAAGGAAAAGTGTTGGT
GGACCAAAAATTGATAAGACTATTGTATTTTCAGAAGACGATAAGAATGATATGGATATC
ACTAAGAGTTATACAATAGAAATAAACCATAGACCTTTATTAGAGAAACGTGATTGTCAT
TTGGTGCCATTGGCAGGAACTTCTGAAACTATTTTATATACATGTAGGCAGGATGACATG
GAGATCACTAGAAGTCACACAACTGCCTTAGAATGTAAAACTGTCTCACCAGATGAAATA
ACTACTAGGCCTATGGACAAAACTGTAGTGTTTGTAGATAATCATGTTGAACTAGAAATG
ACAGAGTCCCATACTGTTTTCATTGACTACCAAGAAAAGGAAAGAACAGACAGACCTAAC
TTTGAACTATCCCAAAGGAAAAGCCTAGGAACACCAACAGTGATATGTACTCCTACTGAG
GAGAGTGTTTTCTTTCCAGGAAATGGTGAAAGTGACCGTCTAGTAGCAAATGACAGCCAG
CTAACCCCTCTGGAGGAATGGTCTAATAATAGGGGCCCTGTAGAGGTAGCTGATAACATG
GAATTGTCTAAATCAGCCACTTGCAAAAACATCAAAGATGTACAAAGTCCTGGATTTCTG
AATGAACCTCTATCAAGCAAAAGTCAGAGAAGAAAAAGCCTTAAGCTAAAAAATGACAAG
ACCATTGTATTTTCAGAGAATCATAAAAATGATATGGATATTACCCAGAGTTGTATGGTG
GAAATAGATAACGAAAGTGCCCTGGAGGATAAAGAGGACTTCCATTTGGCAGGGGCTTCT
AAAACTATTTTGTATTCATGTGGGCAGGATGACATGGAGATCACTAGGAGTCACACAACT
GCCTTAGAATGTAAAACTCTCCTGCCAAATGAAATAGCTATTAGGCCCATGGACAAAACC
GTATTGTTCACAGATAATTACAGTGATCTGGAAGTCACCGATTCCCATACTGTTTTCATT
GACTGTCAAGCCACAGAGAAAATACTTGAAGAAAACCCTAAATTTGGAATAGGAAAAGGA
AAAAACTTGGGTGTTTCCTTTCCTAAGGATAATAGCTGTGTTCAAGAAATCGCTGAAAAA
CAAGCACTGGCTGTAGGAAACAAAATAGTTCTTCACACCGAGCAAAAGCAACAACTCTTT
GCTGCTACTAATAGAACTACTAATGAAATCATCAAATTTCATAGTGCTGCTATGGATGAA
AAGGTCATAGGGAAAGTTGTAGACCAGGCCTGTACATTGGAAAAAGCGCAAGTTGAAAGC
TGTCAGTTAAATAATAGAGATAGAAGAAATGTGGACTTTACAAGTAGTCATGCAACTGCT
GTTTGTGGATCCAGTGATAATTATTCCTGTTTACCAAATGTTATTTCCTGTACTGATAAT
TTGGAGGGTAGTGCCATGCTCTTATGTGATAAAGATGAGGAAAAAGCCAATTATTGCCCA
GTGCAAAATGATCTTGCTTATGCAAATGATTTTGCCAGTGAATATTACTTGGAATCTGAG
GGACAGCCTCTCTCTGCTCCTTGTCCTTTGTTAGAGAAGGAAGAAGTTATTCAAACCAGT
ACCAAAGGACAGTTAGACTGTGTTATAACACTGCACAAAGATCAAGATCTGATTAAGGAT
CCACGAAATCTATTGGCTAATCAAACTTTAGTATATAGTCAAGATCTGGGGGAGATGACT
AAACTTAATTCAAAGCGAGTATCTTTTAAGCTTCCAAAGGATCAAATGAAAGTCTATGTT
GATGACATTTATGTTATTCCTCAGCCTCATTTCTCAACCGACCAACCTCCATTACCTAAA
AAAGGACAGAGTAGTATCAATAAAGAAGAAGTAATACTGTCTAAAGCTGGAAATAAGAGT
TTAAATATTATAGAAAATTCCTCTGCACCCATATGTGAAAACAAGCCCAAAATACTCAAT
AGTGAGGAATGGTTTGCTGCAGCCTGTAAAAAAGAACTGAAGGAAAATATTCAAACAACT
AACTATAATACAGCTCTAGATTTCCACAGTAACTCAGACGTAACTAAGCAAGTCATTCAA
ACTCATGTCAATGCTGGAGAAGCACCAGATCCTGTAATTACATCTAATGTTCCATGTTTT
CATAGTATCAAACCAAATCTGAATAATTTGAATGGAAAAACTGGAGAGTTTTTAGCCTTT
CAAACTGTTCATCTACCACCCCTTCCAGAGCAATTACTTGAATTAGGAAATAAGGCACAC
AATGATATGCATATAGTGCAAGCTACAGAAATACATAATATTAACATAATCTCCAGCAAT
GCTAAAGATAGTAGAGATGAGGAAAATAAAAAGTCTCATAATGGAGCTGAAACCACCTCT
CTACCGCCAAAGACAGTTTTTAAAGATAAAGTAAGGAGATGTTCTTTGGGAATCTTTTTG
CCTAGATTGCCCAACAAGAGAAATTGTAGTGTCACTGGTATTGATGACCTGGAACAGATT
CCAGCAGACACAACTGATATAAATCACTTAGAAACTCAGCCGGTCTCTAGCAAAGATTCA
GGCATTGGATCTGTTGCAGGTAAACTGAACCTAAGTCCTTCTCAATATATAAATGAGGAA
AATCTTCCTGTATATCCTGATGAGATCAATTCTTCAGACTCTATTAACATAGAAACTGAG
GAAAAGGCCTTGATTGAGACATACCAAAAAGAGATTTCACCATATGAAAATAAAATGGGA
AAAACTTGCAATAGCCAAAAAAGAACGTGGGTACAAGAAGAAGAAGATATTCATAAGGAG
AAAAAAATCAGAAAAAATGAGATTAAGTTTAGTGATACGACACAAGATCGGGAGATTTTT
GATCACCATACTGAAGAGGATATAGATAAAAGTGCTAACAGTGTATTGATAAAAAACCTG
AGCAGGACCCCATCTAGTTGCAGCAGCTCTCTGGATTCAATCAAGGCTGATGGGACCTCT
CTGGACTTCAGCACTTACCGCAGTAGTCAAATGGAATCACAGTTTCTCAGAGATACTATT
TGTGAAGAGAGCTTGAGGGAGAAACTCCAAGATGGGAGAATAACAATAAGGGAGTTCTTT
ATACTTCTCCAGGTCCACATCTTGATACAGAAACCCCGACAGAGCAATCTCCCAGGCAAT
TTTACTGTAAACACACCACCTACTCCAGAAGACCTGATGTTAAGTCAATATGTTTACCGA
CCCAAGATACAGATTTATAGAGAAGATTGTGAGGCTCGTCGCCAAAAGATTGAAGAATTA
AAGCTTTCTGCATCGAACCAAGATAAGCTGTTGGTTGATATAAATAAGAACCTGTGGGAA
AAAATGAGACACTGCTCTGACAAAGAGCTGAAGGCCTTTGGAATTTATCTTAACAAAATA
AAGTCATGTTTTACCAAGATGACTAAAGTCTTCACTCACCAAGGAAAAGTGGCTCTGTAT
GGCAAGCTGGTGCAGTCAGCTCAGAATGAGAGGGAGAAACTTCAAATAAAGATAGATGAG
ATGGATAAAATACTTAAGAAGATCGATAACTGCCTCACTGAGATGGAAACAGAAACTAAG
AATTTGGAGGATGAAGAGAAAAACAATCCTGTGGAAGAATGGGATTCTGAAATGAGAGCT
GCAGAAAAAGAATTGGAACAGCTGAAAACTGAAGAAGAGGAGCTTCAAAGAAATCTCTTA
GAACTGGAGGTACAAAAAGAGCAGACCCTTGCTCAAATAGACTTTATGCAAAAACAAAGA
AATAGAACTGAAGAGCTACTGGATCAGTTGAGCTTGTCTGAGTGGGATGTCGTTGAGTGG
AGTGATGATCAAGCTGTATTCACCTTTGTTTATGACACGATACAACTCACCATCACCTTT
GAAGAGTCAGTTGTTGGTTTCCCTTTCCTGGACAAGCGTTATAGGAAGATTGTTGATGTC
AATTTTCAATCTCTGTTAGATGAGGATCAAGCTCCTCCTTCCTCCCTTTTAGTTCATAAG
CTTATTTTCCAGTACGTTGAAGAAAAGGAATCCTGGAAGAAGACATGTACAACCCAGCAT
CAGTTACCCAAGATGCTTGAAGAATTCTCACTGGTAGTGCACCATTGCAGACTCCTTGGA
GAGGAGATTGAGTATTTAAAGAGATGGGGACCAAATTATAACCTAATGAACATAGATATT
AATAATAATGAATTGAGACTTTTATTCTCTAGCTCCGCAGCATTTGCAAAGTTTGAAATA
ACTTTGTTTCTCTCAGCCTATTATCCATCTGTACCATTACCTTCCACCATTCAGAATCAC
GTTGGGAACACTAGCCAAGATGATATTGCTACCATTCTATCTAAAGTGCCACTGGAGAAC
AACTACCTGAAGAATGTAGTCAAGCAAATTTACCAAGATCTGTTTCAGGACTGCCATTTC
TACCACTTG
Protein sequence predicted based on nucleotide sequence
MDGVSSEANEENDNIERPVRRRHSSILKPPRSPLQDLRGGNERVQESNALRNKKNSRRVS FADTIKVFQTESHMKIVRKSEMEGCSAMVPSQLQLLPPGFKRFSCLSLPETETGENLLLI QNKKLEDNYCEITGMNTLLSAPIHTQMQQKEFSIIEHTRERKHANDQTVIFSDENQMDLT SSHTVMITKGLLDNPISEKSTKIDTTSFLANLKLHTEDSRMKKEVNFSVDQNTSSENKID FNDFIKRLKTGKCSAFPDVPDKENFEIPIYSKEPNSASSTHQMHVSLKEDENNSNITRLF REKDDGMNFTQCHTANIQTLIPTSSETNSRESKGNDITIYGNDFMDLTFNHTLQILPATG NFSEIENQTQNAMDVTTGYGTKASGNKTVFKSKQNTAFQDLSINSADKIHITRSHIMGAE THIVSQTCNQDARILAMTPESIYSNPSIQGCKTVFYSSCNDAMEMTKCLSNMREEKNLLK HDSNYAKMYCNPDAMSSLTEKTIYSGEENMDITKSHTVAIDNQIFKQDQSNVQIAAAPTP EKEMMLQNLMTTSEDGKMNVNCNSVPHVSKERIQQSLSNPLSISLTDRKTELLSGENMDL TESHTSNLGSQVPLAAYNLAPESTSESHSQSKSSSDECEEITKSRNEPFQRSDIIAKNSL TDTWNKDKDWVLKILPYLDKDSPQSADCNQEIATSHNIVYCGGVLDKQITNRNTVSWEQS LFSTTKPLFSSGQFSMKNHDTAISSHTVKSVLGQNSKLAEPLRKSLSNPTPDYCHDKMII CSEEEQNMDLTKSHTVVIGFGPSELQELGKTNLEHTTGQLTTMNRQIAVKVEKCGKSPIE KSGVLKSNCIMDVLEDESVQKPKFPKEKQNVKIWGRKSVGGPKIDKTIVFSEDDKNDMDI TKSYTIEINHRPLLEKRDCHLVPLAGTSETILYTCRQDDMEITRSHTTALECKTVSPDEI TTRPMDKTVVFVDNHVELEMTESHTVFIDYQEKERTDRPNFELSQRKSLGTPTVICTPTE ESVFFPGNGESDRLVANDSQLTPLEEWSNNRGPVEVADNMELSKSATCKNIKDVQSPGFL NEPLSSKSQRRKSLKLKNDKTIVFSENHKNDMDITQSCMVEIDNESALEDKEDFHLAGAS KTILYSCGQDDMEITRSHTTALECKTLLPNEIAIRPMDKTVLFTDNYSDLEVTDSHTVFI DCQATEKILEENPKFGIGKGKNLGVSFPKDNSCVQEIAEKQALAVGNKIVLHTEQKQQLF AATNRTTNEIIKFHSAAMDEKVIGKVVDQACTLEKAQVESCQLNNRDRRNVDFTSSHATA VCGSSDNYSCLPNVISCTDNLEGSAMLLCDKDEEKANYCPVQNDLAYANDFASEYYLESE GQPLSAPCPLLEKEEVIQTSTKGQLDCVITLHKDQDLIKDPRNLLANQTLVYSQDLGEMT KLNSKRVSFKLPKDQMKVYVDDIYVIPQPHFSTDQPPLPKKGQSSINKEEVILSKAGNKS LNIIENSSAPICENKPKILNSEEWFAAACKKELKENIQTTNYNTALDFHSNSDVTKQVIQ THVNAGEAPDPVITSNVPCFHSIKPNLNNLNGKTGEFLAFQTVHLPPLPEQLLELGNKAH NDMHIVQATEIHNINIISSNAKDSRDEENKKSHNGAETTSLPPKTVFKDKVRRCSLGIFL PRLPNKRNCSVTGIDDLEQIPADTTDINHLETQPVSSKDSGIGSVAGKLNLSPSQYINEE NLPVYPDEINSSDSINIETEEKALIETYQKEISPYENKMGKTCNSQKRTWVQEEEDIHKE KKIRKNEIKFSDTTQDREIFDHHTEEDIDKSANSVLIKNLSRTPSSCSSSLDSIKADGTS LDFSTYRSSQMESQFLRDTICEESLREKLQDGRITIREFFILLQVHILIQKPRQSNLPGN FTVNTPPTPEDLMLSQYVYRPKIQIYREDCEARRQKIEELKLSASNQDKLLVDINKNLWE KMRHCSDKELKAFGIYLNKIKSCFTKMTKVFTHQGKVALYGKLVQSAQNEREKLQIKIDE MDKILKKIDNCLTEMETETKNLEDEEKNNPVEEWDSEMRAAEKELEQLKTEEEELQRNLL ELEVQKEQTLAQIDFMQKQRNRTEELLDQLSLSEWDVVEWSDDQAVFTFVYDTIQLTITF EESVVGFPFLDKRYRKIVDVNFQSLLDEDQAPPSSLLVHKLIFQYVEEKESWKKTCTTQH QLPKMLEEFSLVVHHCRLLGEEIEYLKRWGPNYNLMNIDINNNELRLLFSSSAAFAKFEI TLFLSAYYPSVPLPSTIQNHVGNTSQDDIATILSKVPLENNYLKNVVKQIYQDLFQDCHF YHL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
