Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860436
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 7323nts
Open reading frame: 1 to 7323
ATGTCAAGTTCAGGTTATCCTCCCAACCAAGGAGCATTCAGCACAGAACAAAGTCGTTAT
CCTCCTCACTCTGTCCAGTATACATTTCCCAACACCCGCCACCAGCAGGAGTTCGCAGTC
CCTGATTATCGTTCCTCTCATCTTGAAGTGAGTCAGGCATCACAGCTTTTGCAGCAACAG
CAGCAGCAACAGCTTCGAAGGCGACCTTCCTTGCTTTCAGAATTTCACCCAGGTTCTGAC
AGGCCTCAAGAAAGGAGAACTAGTTATGAACCGTTTCATCCAGGCCCATCCCCAGTGGAT
CATGATTCACTGGAATCGAAGCGACCACGTCTGGAACAGGTTTCTGATTCTCATTTTCAG
CGTGTCAGTGCTGCGGTTTTGCCTTTAGTGCACCCGCTGCCAGAAGGGCTGAGGGCTTCT
GCAGATGCTAAGAAGGATCCAGCATTCGGAGGCAAACATGAAGCTCCATCCTCTCCAATT
TCGGGGCAACCATGTGGAGATGATCAAAATGCTTCACCTTCAAAACTCTCAAAGGAAGAG
TTAATACAGAGTATGGATCGTGTAGATCGAGAAATTGCAAAAGTAGAACAGCAGATCCTT
AAACTGAAAAAGAAACAACAACAGCTTGAAGAAGAGGCAGCTAAACCTCCTGAGCCTGAG
AAGCCCGTGTCCCCTCCTCCTGTGGAGCAGAAACACCGCAGTATTGTCCAAATTATTTAT
GATGAGAATCGGAAAAAAGCAGAAGAAGCTCATAAAATTTTTGAAGGTCTTGGCCCAAAA
GTTGAACTGCCACTGTATAACCAGCCATCAGATACCAAGGTGTACCATGAGAACATCAAG
ACAAACCAGGTGATGAGGAAAAAACTCATTTTATTTTTTAAAAGAAGAAATCATGCAAGA
AAACAAAGGGAACAAAAAATCTGCCAGCGTTATGATCAGCTCATGGAGGCATGGGAGAAA
AAAGTGGACAGAATAGAAAATAATCCTCGGAGGAAAGCTAAAGAAAGCAAAACAAGGGAA
TACTATGAAAAGCAGTTTCCAGAAATTCGAAAACAAAGAGAACAGCAAGAAAGATTTCAG
CGAGTTGGGCAGAGGGGAGCTGGTCTTTCAGCCACCATTGCTAGGAGTGAGCATGAGATT
TCTGAAATTATTGATGGGCTCTCTGAGCAGGAGAATAATGAGAAACAAATGCGGCAGCTC
TCTGTGATTCCACCTATGATGTTTGATGCAGAACAAAGACGAGTCAAGTTCATTAACATG
AATGGGCTTATGGAGGACCCTATGAAAGTGTATAAAGATAGGCAGTTTATGAATGTTTGG
ACTGACCATGAAAAGGAGATCTTTAAGGACAAGTTTATCCAGCATCCAAAAAACTTTGGA
CTAATTGCATCATACTTGGAGAGGAAGAGTGTTCCTGATTGTGTTTTGTATTACTATTTA
ACCAAGAAAAATGAGAATTATAAAGCCCTCGTCAGAAGGAATTATGGGAAACGCAGAGGC
AGAAACCAGCAAATTGCTCGACCCTCGCAAGAAGAAAAAGTAGAAGAAAAAGAAGAGGAT
AAAGCAGAAAAAACAGAAAAAAAAGAAGAAGAAAAGAAAGATGAAGAGGAAAAAGATGAA
AAAGAAGACTCCAAAGAAAATACCAAGGAAAAGGACAAGATAGATGGTACAGCAGAAGAA
ACTGAGGAAAGAGAGCAAGCCACACCCCGGGGGCGAAAGACTGCCAACAGTCAGGGCCGC
CGTAAGGGCCGGATCACCAGGTCCATGACAAACGAAGCTGCAGCTGCCAGTGCTGCAGCC
GCAGCGGCTACTGAAGAGCCCCCACCACCTCTGCCACCGCCACCAGAACCCATTTCTACA
GAGCCTGTGGAGACCTCTCGATGGACAGAAGAAGAAATGGAAGTTGCTAAAAAAGGTCTA
GTAGAACATGGTCGTAACTGGGCAGCAATTGCTAAAATGGTGGGAACGAAAAGTGAAGCT
CAATGTAAAAACTTCTATTTTAACTATAAAAGGCGACACAATCTTGACAACCTCTTACAG
CAGCATAAACAGAAAACTTCACGAAAACCTCGTGAAGAGCGAGATGTGTCTCAATGTGAA
AGTGTCGCTTCCACTGTTTCTGCTCAGGAGGATGAAGATATTGAAGCCTCCAATGAAGAA
GAAAATCCAGAAGACAGCGAAGTTGAAGCTGTCAAGCCCAGCGAGGACAGTCCTGAAAAT
GCTACTTCTCGAGGAAACACAGAACCTGCGGTTGAGCTTGAGCCCACCACGGAAACTGCA
CCCAGTACATCTCCCTCCTTAGCAGTTCCAAGTACAAAACCAGCTGAAGATGAAAGTGTG
GAGACCCAGGTGAATGACAGCATCAGTGCTGAGACAGCAGAGCAGATGGATGTAGATCAG
CAGGAGCACAGTGCTGAAGAGGGTTCTGTTTGTGATCCCCCACCCGCTACCAAAGCTGAC
TCTGTGGACGTTGAAGTGAGGGTGCCAGAAAACCATGCATCTAAAGTTGAAGGTGATAAT
ACCAAAGAAAGAGACTTGGATAGAGCCAGTGAGAAGGTGGAACCTAGAGATGAAGATTTG
GTGGTAGCTCAGCAAATAAATGCCCAAAGGCCCGAGCCCCAGTCAGACAATGATTCCAGT
GCCACGTGCAGCGCTGATGAGGATGTGGATGGAGAGCCAGAGAGGCAGAGAATGTTTCCT
ATGGACTCAAAGCCTTCACTGTTAAACCCCACTGGATCTATACTCGTCTCATCTCCGTTA
AAACCAAATCCACTGGATCTGCCACAGCTTCAGCATCGAGCTGCTGTTATCCCACCAATG
GTATCCTGCACCCCATGTAACATACCAATTGGAACCCCAGTGAGCGGCTATGCTCTCTAC
CAGCGACACATTAAAGCAATGCATGAGTCAGCACTCCTGGAGGAGCAGCGGCAGAGACAA
GAACAGATAGATTTGGAATGTAGAAGTTCTACAAGTCCATGTGGCACATCCAAGAGTCCA
AACAGAGAGTGGGAAGTCCTTCAGCCTGCTCCACATCAAGTGATAACTAATCTCCCTGAA
GGCGTTCGGCTTCCGACAACTCGACCAACCAGGCCACCGCCCCCTCTCATCCCGTCATCC
AAAACCACAGTGGCTTCAGAAAAACCATCTTTTATAATGGGAGGCTCCATCTCACAGGGA
ACACCAGGCACTTATTTGACTTCTCATAATCAGGCTTCCTACACTCAAGAAACACCCAAG
CCGTCAGTGGGATCTATCTCTCTTGGACTGCCACGGCAACAGGAATCTGCCAAATCAGCT
ACTTTGCCCTACATCAAGCAGGAAGAATTTTCTCCCCGAAGCCAAAACTCACAACCTGAG
GGTCTGTTGGTCAGGGCCCAACATGAAGGTGTAGTCAGAGGTACCGCAGGAGCCATACAA
GAAGGAAGTATAACTCGGGGAACTCCAACCAGCAAAATTTCAGTGGAGAGCATTCCATCC
CTACGGGGCTCTATCACTCAGGGCACCCCGGCTCTGCCCCAGACTGGCATACCAACAGAG
GCTTTGGTGAAGGGGTCCATTTCGAGAATGCCCATTGAAGACAGCAGTCCTGAGAAAGGC
AGAGAGGAAGCTGCATCCAAAGGCCATGTTATTTATGAAGGCAAAAGTGGACATATCTTG
TCATATGATAATATTAAGAATGCCCGAGAAGGGACTAGGAGTCCAAGAACAGCTCATGAA
ATCAGTTTAAAGAGAAGCTATGAATCAGTGGAAGGAAATATAAAGCAAGGGATGTCAATG
AGGGAGTCTCCTGTATCAGCACCGTTAGAGGGGCTGATATGCCGAGCATTACCCAGGGGG
AGTCCTCATTCTGACCTCAAAGAAAGGACTGTATTGTCTGGCTCCATAATGCAGGGGACA
CCAAGAGCAACAACTGAAAGCTTTGAAGATGGCCTTAAATATCCCAAACAAATTAAAAGG
GAAAGTCCTCCCATACGAGCATTTGAAGGTGCCATTACCAAAGGAAAACCATATGATGGC
ATCACCACCATCAAAGAAATGGGGCGTTCCATTCATGAGATTCCAAGGCAAGATATTTTA
ACTCAGGAAAGTCGGAAAACTCCAGAAGTGGTCCAGAGCACACGGCCGATAATTGAGGGT
TCCATTTCCCAGGGCACACCAATAAAGTTTGACAACAACTCAGGTCAATCTGCCATCAAA
CACAATGTCAAATCCTTAATCACGGGGCCTAGCAAACTATCCCGTGGAATGCCTCCGCTG
GAAATTGTGCCAGAGAACATAAAAGTGGTAGAACGGGGAAAATATGAGGATGTGAAAGCA
GGCGAGACCGTGCGTTCCCGGCACACGTCAGTGGTAAGCTCTGGCCCCTCCGTTCTTAGG
TCCACACTGCATGAAGCTCCCAAAGCACAACTGAGCCCTGGGATTTATGATGACACCAGT
GCACGGAGGACCCCTGTGAGTTATCAAAACACCATGTCCAGAGGCTCACCCATGATGAAC
AGAACTTCTGATGTTACAATTTCTTCTAACAAGTCTACCAATCATGAAAGGAAATCGACA
CTGACCCCTACCCAGAGGGAAAGTATCCCAGCGAAGTCTCCAGTGCCTGGGGTGGACCCT
GTCGTGAGCCACAGTCCGTTTGATCCCCATCACAGAGGCAGCACTGCAGGCGAGGTTTAT
CGGAGCCACCTGCCCACGCACTTGGATCCAGCCATGCCTTTTCACAGGGCTTTGGATCCT
GCAGCGGCTGCTTACCTGTTTCAGAGACAGCTTTCACCAACTCCAGGTTACCCAAGTCAG
TATCAGCTTTACGCAATGGAGAACACAAGACAGACAATCTTAAATGATTACATTACCTCA
CAACAGATGCAAGTGAACTTGCGTCCAGATGTGGCCAGAGGACTCTCCCCAAGAGAGCAG
CCACTGGGTCTCCCATACCCAGCAACGAGAGGAATCATTGACCTGACCAATATGCCTCCA
ACAATTTTAGTGCCTCATCCAGGGGGAACAAGCACTCCTCCCATGGACAGAATCACTTAT
ATTCCTGGTACACAGATTACTTTCCCTCCCAGGCCGTACAACTCTGCTTCCATGTCTCCA
GGACACCCAACACACCTTGCAGCTGCTGCAAGTGCTGAGAGGGAACGGGAACGGGAGCGG
GAGAAGGAGCGGGAGCGGGAACGGATTGCTGCAGCTTCCTCCGACCTCTACCTGCGGCCA
GGCTCAGAACAGCCTGGCCGACCTGGCAGTCATGGATATGTTCGCTCCCCTTCCCCTTCA
GTAAGAACTCAGGAGACCATGTTGCAACAGAGACCCAGTGTTTTCCAAGGAACCAATGGA
ACCAGTGTAATCACACCTTTGGATCCAACTGCTCAGCTACGAATCATGCCACTGCCTGCT
GGGGGCCCTTCAATAAGCCAAGGCCTGCCAGCCTCCCGTTACAACACTGCTGCGGATGCC
CTGGCTGCTCTTGTGGATGCTGCAGCTTCTGCACCCCAGATGGATGTGTCCAAAACAAAA
GAGAGTAAGCATGAAGCTGCCAGGTTAGAAGAAAATTTGAGAAGCAGGTCAGCAGCAGTT
AGTGAACAGCAGCAGCTAGAGCAGAAAACCCTGGAGGTGGAGAAGAGATCTGTTCAGTGT
TTATACACTTCTTCAGCCTTTCCAAGTGGCAAGCCCCAGCCTCATTCTTCAGTAGTTTAT
TCTGAGGCTGGGAAAGATAAAGGGCCTCCTCCAAAATCCAGATATGAGGAAGAGCTAAGG
ACCAGAGGGAAGACTACCATTACTGCAGCTAACTTCATAGACGTGATCATCACCCGGCAA
ATTGCCTCGGACAAGGATGCGAGGGAACGTGGCTCTCAAAGTTCAGACTCTTCTAGTAGC
TTATCTTCTCACAGGTATGAAACACCTAGCGATGCTATTGAGGTGATAAGTCCTGCCAGC
TCACCTGCGCCACCCCAGGAGAAACTGCAGACCTATCAGCCAGAGGTTGTTAAGGCAAAT
CAAGCGGAAAATGATCCTACCAGACAATATGAAGGACCATTACATCACTATCGACCACAG
CAGGAATCACCATCTCCCCAACAACAGCTGCCCCCTTCTTCACAGGCAGAGGGAATGGGG
CAAGTGCCCAGGACCCATCGGCTGATCACACTTGCTGATCACATCTGTCAAATTATCACA
CAAGATTTTGCTAGAAATCAAGTTTCCTCGCAGACTCCCCAGCAGCCTCCTACTTCTACA
TTCCAGAACTCACCTTCTGCTTTGGTATCTACACCTGTGAGGACTAAAACATCAAACCGT
TACAGCCCAGAATCCCAGGCTCAGTCTGTCCATCATCAAAGACCAGGTTCAAGGGTCTCT
CCAGAAAATCTTGTGGACAAATCCAGGGGAAGTAGGCCTGGAAAATCCCCAGAGAGGAGT
CACGTCTCTTCGGAGCCCTACGAGCCCATCTCCCCACCCCAGGTTCCGGTTGTGCATGAG
AAACAGGACAGCTTGCTGCTCTTGTCTCAGAGGGGCGCAGAGCCTGCAGAGCAGAGGAAT
GATGCCCGCTCACCAGGGAGTATAAGCTACTTGCCTTCATTCTTCACCAAGCTTGAAAAT
ACATCACCCATGGTTAAATCAAAGAAGCAGGAGATTTTTCGTAAGTTGAACTCCTCTGGT
GGAGGTGACTCTGATATGGCAGCTGCTCAGCCAGGAACTGAGATCTTTAATCTGCCAGCA
GTTACTACGTCAGGCTCAGTTAGCTCTAGAGGCCATTCTTTTGCTGATCCTGCCAGTAAT
CTTGGGCTGGAAGACATTATCAGGAAGGCTCTCATGGGAAGCTTTGATGACAAAGTTGAG
GATCATGGAGTTGTCATGTCCCAGCCTATGGGAGTAGTGCCTGGTACTGCCAACACCTCA
GTTGTGACCAGTGGTGAGACACGAAGAGAGGAAGGGGACCCATCACCTCATTCAGGAGGA
GTTTGCAAACCAAAGCTGATCAGCAAGTCAAACAGCAGGAAATCTAAGTCTCCTATACCT
GGGCAAGGCTACTTAGGAACGGAACGGCCCTCTTCAGTCTCCTCTGTACATTCAGAAGGG
GATTACCATAGGCAGACGCCAGGGTGGGCCTGGGAAGACAGGCCCTCTTCAACAGGCTCA
ACTCAGTTTCCTTATAACCCTCTGACTATGCGGATGCTCAGCAGTACTCCACCAACACCG
ATTGCATGTGCTCCCTCTGCGGTGAACCAAGCAGCTCCTCACCAACAGAACAGGATCTGG
GAGCGAGAGCCTGCCCCACTGCTCTCAGCACAGTACGAGACCCTGTCGGATAGTGATGAC
TTG
Protein sequence predicted based on nucleotide sequence
MSSSGYPPNQGAFSTEQSRYPPHSVQYTFPNTRHQQEFAVPDYRSSHLEVSQASQLLQQQ QQQQLRRRPSLLSEFHPGSDRPQERRTSYEPFHPGPSPVDHDSLESKRPRLEQVSDSHFQ RVSAAVLPLVHPLPEGLRASADAKKDPAFGGKHEAPSSPISGQPCGDDQNASPSKLSKEE LIQSMDRVDREIAKVEQQILKLKKKQQQLEEEAAKPPEPEKPVSPPPVEQKHRSIVQIIY DENRKKAEEAHKIFEGLGPKVELPLYNQPSDTKVYHENIKTNQVMRKKLILFFKRRNHAR KQREQKICQRYDQLMEAWEKKVDRIENNPRRKAKESKTREYYEKQFPEIRKQREQQERFQ RVGQRGAGLSATIARSEHEISEIIDGLSEQENNEKQMRQLSVIPPMMFDAEQRRVKFINM NGLMEDPMKVYKDRQFMNVWTDHEKEIFKDKFIQHPKNFGLIASYLERKSVPDCVLYYYL TKKNENYKALVRRNYGKRRGRNQQIARPSQEEKVEEKEEDKAEKTEKKEEEKKDEEEKDE KEDSKENTKEKDKIDGTAEETEEREQATPRGRKTANSQGRRKGRITRSMTNEAAAASAAA AAATEEPPPPLPPPPEPISTEPVETSRWTEEEMEVAKKGLVEHGRNWAAIAKMVGTKSEA QCKNFYFNYKRRHNLDNLLQQHKQKTSRKPREERDVSQCESVASTVSAQEDEDIEASNEE ENPEDSEVEAVKPSEDSPENATSRGNTEPAVELEPTTETAPSTSPSLAVPSTKPAEDESV ETQVNDSISAETAEQMDVDQQEHSAEEGSVCDPPPATKADSVDVEVRVPENHASKVEGDN TKERDLDRASEKVEPRDEDLVVAQQINAQRPEPQSDNDSSATCSADEDVDGEPERQRMFP MDSKPSLLNPTGSILVSSPLKPNPLDLPQLQHRAAVIPPMVSCTPCNIPIGTPVSGYALY QRHIKAMHESALLEEQRQRQEQIDLECRSSTSPCGTSKSPNREWEVLQPAPHQVITNLPE GVRLPTTRPTRPPPPLIPSSKTTVASEKPSFIMGGSISQGTPGTYLTSHNQASYTQETPK PSVGSISLGLPRQQESAKSATLPYIKQEEFSPRSQNSQPEGLLVRAQHEGVVRGTAGAIQ EGSITRGTPTSKISVESIPSLRGSITQGTPALPQTGIPTEALVKGSISRMPIEDSSPEKG REEAASKGHVIYEGKSGHILSYDNIKNAREGTRSPRTAHEISLKRSYESVEGNIKQGMSM RESPVSAPLEGLICRALPRGSPHSDLKERTVLSGSIMQGTPRATTESFEDGLKYPKQIKR ESPPIRAFEGAITKGKPYDGITTIKEMGRSIHEIPRQDILTQESRKTPEVVQSTRPIIEG SISQGTPIKFDNNSGQSAIKHNVKSLITGPSKLSRGMPPLEIVPENIKVVERGKYEDVKA GETVRSRHTSVVSSGPSVLRSTLHEAPKAQLSPGIYDDTSARRTPVSYQNTMSRGSPMMN RTSDVTISSNKSTNHERKSTLTPTQRESIPAKSPVPGVDPVVSHSPFDPHHRGSTAGEVY RSHLPTHLDPAMPFHRALDPAAAAYLFQRQLSPTPGYPSQYQLYAMENTRQTILNDYITS QQMQVNLRPDVARGLSPREQPLGLPYPATRGIIDLTNMPPTILVPHPGGTSTPPMDRITY IPGTQITFPPRPYNSASMSPGHPTHLAAAASAEREREREREKERERERIAAASSDLYLRP GSEQPGRPGSHGYVRSPSPSVRTQETMLQQRPSVFQGTNGTSVITPLDPTAQLRIMPLPA GGPSISQGLPASRYNTAADALAALVDAAASAPQMDVSKTKESKHEAARLEENLRSRSAAV SEQQQLEQKTLEVEKRSVQCLYTSSAFPSGKPQPHSSVVYSEAGKDKGPPPKSRYEEELR TRGKTTITAANFIDVIITRQIASDKDARERGSQSSDSSSSLSSHRYETPSDAIEVISPAS SPAPPQEKLQTYQPEVVKANQAENDPTRQYEGPLHHYRPQQESPSPQQQLPPSSQAEGMG QVPRTHRLITLADHICQIITQDFARNQVSSQTPQQPPTSTFQNSPSALVSTPVRTKTSNR YSPESQAQSVHHQRPGSRVSPENLVDKSRGSRPGKSPERSHVSSEPYEPISPPQVPVVHE KQDSLLLLSQRGAEPAEQRNDARSPGSISYLPSFFTKLENTSPMVKSKKQEIFRKLNSSG GGDSDMAAAQPGTEIFNLPAVTTSGSVSSRGHSFADPASNLGLEDIIRKALMGSFDDKVE DHGVVMSQPMGVVPGTANTSVVTSGETRREEGDPSPHSGGVCKPKLISKSNSRKSKSPIP GQGYLGTERPSSVSSVHSEGDYHRQTPGWAWEDRPSSTGSTQFPYNPLTMRMLSSTPPTP IACAPSAVNQAAPHQQNRIWEREPAPLLSAQYETLSDSDDL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
