Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860502
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6348nts
Open reading frame: 1 to 6348
ATGACACTCCACGCCACCCGGGGGGCTGCACTCCTCTCTTGGGTGAACAGTCTACACGTG
GCTGACCCTGTGGAGGCTGTGCTGCAGCTCCAGGACTGCAGCATCTTCATCAAGATCATT
GACAGAATCCATGGCACTGAAGAGGGACAGCAAATCTTGAAGCAGCCGGTGTCAGAGAGA
CTGGACTTTGTGTGCAGTTTTCTGCAGAAAAATCGAAAACATCCCTCTTCCCCAGAATGC
CTGGTATCTGCACAGAAGGTGCTAGAGGGATCAGAGCTGGAACTGGCGAAGATGACCATG
CTGCTCTTATACCACTCTACCATGAGCTCCAAAAGTCCCAGGGACTGGGAACAGTTTGAA
TATAAAATTCAGGCTGAGTTGGCTGTCATTCTTAAATTTGTGCTGGACCATGAGGACGGG
CTAAACCTTAATGAGGACCTAGAGAACTTCCTACAGAAAGCTCCTGTGCCTTCTACCTGT
TCTAGCACATTCCCTGAAGAGCTCTCCCCACCTAGCCACCAGGCCAAGAGGGAGATTCGC
TTCCTAGAGCTACAGAAGGTTGCCTCCTCTTCCAGTGGGAACAACTTTCTCTCAGGTTCT
CCAGCTTCTCCCATGGGTGATATCCTGCAGACCCCACAGTTCCAGATGAGACGGCTGAAG
AAGCAGCTTGCTGATGAGAGAAGTAATAGGGATGAGCTGGAGCTGGAGCTAGCTGAGAAC
CGCAAGCTCCTCACCGAGAAGGATGCACAGATAGCCATGATGCAGCAGCGCATTGACCGC
CTAGCCCTGCTGAATGAGAAGCAGGCGGCCAGCCCACTGGAGCCCAAGGAGCTTGAGGAG
CTGCGTGACAAGAATGAGAGCCTTACCATGCGGCTGCATGAAACCCTGAAGCAGTGCCAG
GACCTGAAGACAGAGAAGAGCCAGATGGATCGCAAAATCAACCAGCTTTCGGAGGAGAAT
GGAGACCTTTCCTTTAAGCTGCGGGAGTTTGCCAGTCATCTGCAGCAGCTACAGGATGCC
CTCAATGAGCTGACGGAGGAGCACAGCAAGGCCACTCAGGAGTGGCTAGAGAAGCAGGCC
CAGCTGGAGAAGGAGCTCAGCGCAGCCCTGCAGGACAAGAAATGCCTTGAAGAGAAGAAC
GAAATCCTTCAGGGAAAACTTTCACAGCTGGAAGAACACTTGTCCCAGCTGCAGGATAAC
CCACCCCAGGAGAAGGGCGAGGTGCTGGGTGATGTCTTGCAGCTGGAAACCTTGAAGCAA
GAGGCAGCCACTCTTGCTGCAAACAACACACAGCTCCAAGCCAGGGTAGAGATGCTGGAG
ACTGAGCGAGGCCAGCAGGAAGCCAAGCTGCTTGCTGAGCGGGGCCACTTCGAAGAAGAA
AAGCAGCAGCTGTCTAGCCTGATCACTGACCTGCAGAGCTCCATCTCCAACCTCAGCCAG
GCCAAGGAAGAGCTGGAGCAGGCCTCCCAGGCTCATGGGGCCCGGTTGACTGCCCAGGTG
GCCTCTCTGACCTCTGAGCTCACCACACTCAATGCCACCATCCAGCAACAGGATCAAGAA
CTGGCTGGCCTGAAGCAGCAGGCCAAAGAGAAGCAGGCCCAGCTAGCACAGACCCTCCAA
CAGCAAGAACAGGCCTCCCAGGGCCTCCGCCACCAGGTGGAGCAGCTAAGCAGTAGCCTG
AAGCAGAAGGAGCAGCAGTTGAAGGAGGTAGCGGAGAAGCAGGAGGCAACTAGGCAGGAC
CATGCCCAGCAACTGGCCACTGCTGCAGAGGAGCGAGAGGCCTCCTTAAGGGAGCGGGAT
GCGGCTCTCAAGCAGCTGGAGGCACTGGAGAAGGAGAAGGCTGCCAAGCTGGAGATTCTG
CAGCAGCAACTTCAGGTGGCTAATGAAGCCCGGGACAGTGCCCAGACCTCAGTGACACAG
GCCCAGCGGGAGAAGGCAGAGCTGAGCCGGAAGGTGGAGGAACTCCAGGCCTGTGTTGAG
ACAGCCCGCCAGGAACAGCATGAGGCCCAGGCCCAGGTTGCAGAGCTAGAGTTGCAGCTG
CGGTCTGAGCAGCAAAAAGCAACTGAGAAAGAAAGGGTGGCCCAGGAGAAGGACCAGCTC
CAGGAGCAGCTCCAGGCCCTCAAAGAGTCCTTGAAGGTCACCAAGGGCAGCCTTGAAGAG
GAGAAGCGCAGGGCTGCAGATGCCCTGGAAGAGCAGCAGCGTTGTATCTCTGAGCTGAAG
GCAGAGACCCGAAGCCTGGTGGAGCAGCATAAGCGGGAACGAAAGGAGCTGGAAGAAGAG
AGGGCTGGGCGCAAGGGGCTGGAGGCTCGATTACAGCAGCTTGGGGAGGCCCATCAGGCT
GAGACTGAAGTCCTGCGGCGGGAGCTGGCAGAGGCCATGGCTGCCCAGCACACAGCTGAG
AGTGAGTGTGAGCAGCTCGTCAAAGAAGTAGCTGCCTGGCGTGAGCGGTATGAGGATAGC
CAGCAAGAGGAGGCACAGTATGGCGCCATGTTCCAGGAACAGCTGATGACTTTGAAGGAG
GAATGTGAGAAGGCCCGCCAGGAGCTGCAGGAGGCAAAGGAGAAGGTGGCAGGCATAGAA
TCCCACAGCGAGCTCCAGATAAGCCGGCAGCAGAACGAACTAGCTGAGCTCCATGCCAAC
CTGGCCAGAGCACTCCAGCAGGTCCAAGAGAAGGAAGTCAGGGCCCAGAAGCTTGCAGAT
GACCTCTCCACTCTGCAGGAAAAGATGGCTGCCACCAGCAAAGAGGTGGCCCGCTTGGAG
ACCTTGGTGCGCAAGGCAGGTGAGCAGCAGGAAACAGCCTCCCGGGAGTTAGTCAAGGAG
CCTGCGAGGGCAGGAGACAGACAGCCCGAGTGGCTGGAAGAGCAACAGGGACGCCAGTTC
TGCAGCACACAGGCAGCGCTGCAGGCTATGGAGCGGGAGGCAGAGCAGATGGGCAATGAG
CTGGAACGGCTGCGGGCCGCGCTGATGGAGAGCCAGGGGCAGCAGCAGGAGGAGCGTGGG
CAGCAGGAAAGGGAGGTGGCGCGGCTGACCCAGGAGCGGGGCCGTGCCCAGGCTGACCTT
GCCCTGGAGAAGGCGGCCAGAGCAGAGCTTGAGATGCGGCTGCAGAACGCCCTCAACGAG
CAGCGTGTGGAGTTCGCTACCCTGCAAGAGGCACTGGCTCATGCCCTGACGGAAAAGGAA
GGCAAGGACCAGGAGTTGGCCAAGCTTCGTGGTCTGGAGGCAGCCCAGATAAAAGAGCTG
GAGGAACTTCGGCAAACCGTGAAGCAACTGAAGGAACAGCTGGCTAAGAAAGAAAAGGAG
CACGCATCTGGCTCAGGAGCCCAATCTGAGGCTGCTGGCAGGACAGAGCCAACAGGCCCC
AAGCTGGAGGCACTGCGGGCAGAGGTGAGCAAGCTGGAACAGCAATGCCAGAAGCAGCAG
GAGCAGGCTGACAGCCTGGAACGCAGCCTCGAGGCTGAGCGGGCCTCCCGGGCTGAGCGG
GACAGTGCTCTGGAGACTCTGCAGGGCCAGTTAGAGGAGAAGGCCCAGGAGCTAGGGCAC
AGTCAGAGTGCCTTAGCCTCGGCCCAACGGGAGTTGGCTGCCTTCCGCACCAAGGTACAA
GACCACAGCAAGGCTGAAGATGAGTGGAAGGCCCAGGTGGCCCGGGGCCGGCAAGAGGCT
GAGAGGAAAAATAGCCTCATCAGCAGCTTGGAGGAGGAGGTGTCCATCCTGAATCGCCAG
GTCCTGGAGAAGGAGGGGGAGAGCAAGGAGTTGAAGCGGCTGGTGATGGCCGAGTCAGAG
AAGAGCCAGAAGCTGGAGGAGAGGCTGCGCCTGCTGCAGGCAGAGACAGCCAGCAACAGT
GCCAGAGCTGCAGAACGCAGCTCTGCTCTGCGGGAGGAGGTGCAGAGCCTCCGGGAGGAG
GCTGAGAAACAGCGGGTGGCTTCAGAGAACCTGCGGCAGGAGCTGACCTCACAGGCTGAG
CGTGCGGAGGAGCTGGGCCAAGAATTGAAGGCGTGGCAGGAGAAGTTCTTCCAGAAAGAG
CAGGCCCTCTCCACCCTGCAGCTCGAGCACACCAGCACACAGGCCCTGGTGAGTGAGCTG
CTGCCAGCTAAGCACCTCTGCCAGCAGCTGCAGGCCGAGCAGGCCGCTGCCGAGAAACGC
CACCGTGAGGAGCTGGAGCAGAGCAAGCAGGCCGCTGGGGGACTGCGGGCAGAGCTGCTG
CGGGCCCAGCGGGAGCTTGGGGAGCTGATTCCTCTGCGGCAGAAGGTGGCAGAGCAGGAG
CGAACAGCTCAGCAGCTGCGGGCAGAGAAGGCCAGCTATGCAGAGCAGCTGAGCATGCTG
AAGAAGGCGCATGGCCTGCTGGCAGAGGAGAACCGGGGGCTGGGTGAGCGGGCCAACCTT
GGCCGGCAGTTTCTGGAAGTGGAGTTGGACCAGGCCCGGGAGAAGTATGTCCAAGAGTTG
GCAGCCGTACGTGCTGATGCTGAGACCCGTCTGGCTGAGGTGCAGCGAGAAGCACAGAGC
ACTGCCCGGGAGCTGGAGGTGATGACTGCCAAGTATGAGGGTGCCAAGGTCAAGGTCCTG
GAGGAGAGGCAGCGGTTCCAGGAAGAGAGGCAGAAACTCACTGCCCAGGTGGAGCAGCTA
GAGGTATTTCAGAGAGAGCAAACTAAGCAGGTGGAAGAACTGAGTAAGAAACTGGCTGAC
TCTGACCAAGCCAGCAAGGTGCAGCAGCAGAAGCTGAAGGCTGTCCAGGCTCAGGGAGGC
GAGAGCCAGCAGGAGGCCCAGCGCCTCCAGGCCCAGCTGAATGAACTGCAAGCCCAGTTG
AGCCAGAAGGAGCAGGCAGCTGAGCACTATAAGCTGCAGATGGAGAAAGCCAAAACACAT
TATGATGCCAAGAAGCAGCAGAACCAAGAGCTGCAGGAGCAGCTGCGGAGCCTGGAGCAG
CTGCAGAAGGAAAACAAAGAGCTGCGAGCTGAAGCTGAACGGCTGGGCCATGAGCTACAG
CAGGCTGGGCTGAAGACCAAGGAGGCTGAACAGACCTGCCGCCACCTTACTGCCCAGGTG
CGCAGCCTGGAGGCACAGGTTGCCCATGCAGACCAGCAGCTTCGAGACCTGGGCAAATTC
CAGGTGGCAACTGATGCTTTAAAGAGCCGTGAGCCCCAGGCTAAGCCCCAGCTGGACTTG
AGTATTGACAGCCTGGATCTGAGCTGCGAGGAGGGGACCCCACTCAGTATCACCAGCAAG
CTGCCTCGTACCCAGCCAGACGGCACCAGCGTCCCTGGAGAACCAGCCTCACCTATCTCC
CAGCGCCTGCCCCCCAAGGTAGAATCCCTGGAGAGTCTCTACTTCACTCCCATCCCTGCT
CGGAGTCAGGCCCCCCTGGAGAGCAGCCTGGACTCCCTGGGAGACGTCTTCCTGGACTCG
GGTCGTAAGACCCGCTCCGCTCGTCGGCGCACCACGCAGATCATCAACATCACCATGACC
AAGAAGCTAGATGTGGAAGAGCCAGACAGCGCCAACTCATCGTTCTACAGCACGCGGTCT
GCTCCTGCTTCCCAGGCTAGCCTGCGAGCCACCTCCTCTACTCAGTCTCTAGCTCGCCTG
GGTTCTCCCGATTATGGCAACTCAGCCCTGCTCAGCTTGCCTGGCTACCGCCCCACCACT
CGCAGTTCTGCTCGTCGTTCCCAGGCCGGGGTGTCCAGTGGGGCCCCTCCAGGAAGGAAC
AGCTTCTACATGGGCACTTGCCAGGATGAGCCTGAGCAGCTGGATGACTGGAACCGCATT
GCAGAGCTGCAGCAGCGCAATCGAGTGTGCCCCCCACATCTGAAGACCTGCTATCCCCTG
GAGTCCAGGCCTTCCCTGAGCCTGGGCACCATCACAGATGAGGAGATGAAAACTGGAGAC
CCCCAAGAGACCCTGCGCCGAGCCAGCATGCAGCCAATCCAGATAGCCGAGGGCACTGGC
ATCACCACCCGGCAGCAGCGCAAACGGGTCTCCCTAGAGCCCCACCAGGGCCCTGGAACT
CCTGAGTCTAAGAAGGCCACCAGCTGTTTCCCACGCCCCATGACTCCCCGAGACCGACAT
GAAGGGCGCAAACAGAGCACTACTGAGGCCCAGAAGAAAGCAGCTCCAGCTTCTACTAAA
CAGGCTGACCGGCGCCAGTCGATGGCCTTCAGCATCCTCAACACACCCAAGAAGCTAGGG
AACAGCCTTCTGCGGCGGGGAGCCTCAAAGAAGGCCCTGTCCAAGGCTTCCCCCAACACT
CGCAGTGGAACCCGCCGTTCTCCGCGCATTGCCACCACCACAGCCAGCGCCGCCACTGCT
GCCGCCATTGGTGCCACCCCTCGAGCCAAGGGCAAGGCAAAGCACTTG
Protein sequence predicted based on nucleotide sequence
MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSER LDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFE YKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIR FLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAEN RKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQ DLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQA QLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQ EAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQ AKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQ QQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERD AALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVE TARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEE EKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQA ETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKE ECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLAD DLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQF CSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADL ALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKEL EELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQ EQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQ DHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESE KSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAE RAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKR HREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSML KKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQS TARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLAD SDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTH YDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQV RSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITSK LPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDS GRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARL GSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRI AELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTG ITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTK QADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATA AAIGATPRAKGKAKHL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
