Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860530
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4362nts
Open reading frame: 1 to 4362
ATGAAATCCTGCGGAGTGTCGCTCGCTACCGCCGCCGCTGCCGCCGCCGCTTTCGGTGAT
GAGGAAAAGAAAATGGCGGCGGGAAAAGCGAGCGGCGAGAGCGAGGAGGCGTCCCCCAGC
CTGACAGCCGAGGAGAGGGAGGCGCTCGGCGGACTGGACAGCCGCCTCTTTGGGTTCGTG
AGATTTCATGAAGATGGCGCCAGGACGAAGGCCCTACTGGGCAAGGCTGTTCGCTGCTAT
GAATCTCTAATCTTAAAAGCTGAAGGAAAAGTGGAGTCTGATTTCTTTTGTCAATTAGGT
CACTTCAACCTCTTATTGGAAGATTATCCAAAAGCATTATCTGCATACCAGAGGTACTAC
AGTTTACAGTCTGACTACTGGAAGAATGCTGCCTTTTTATATGGTCTTGGTTTGGTCTAC
TTCCATTATAATGCATTTCAGTGGGCAATTAAAGCATTTCAGGAGGTGCTTTATGTTGAT
CCCAGCTTTTGTCGAGCCAAGGAAATTCATTTACGACTTGGGCTTATGTTCAAAGTGAAC
ACAGACTATGAGTCTAGTTTAAAGCATTTTCAGTTAGCTTTGGTTGACTGTAATCCCTGC
ACTTTGTCCAATGCTGAAATTCAATTTCACATTGCCCACTTATATGAAACCCAGAGGAAA
TATCATTCTGCAAAAGAAGCTTATGAACAACTTTTGCAGACAGAGAATCTTTCTGCACAA
GTAAAAGCAACTGTCTTACAACAGTTAGGTTGGATGCATCACACTGTAGATCTCCTGGGA
GATAAAGCCACCAAGGAAAGCTATGCTATTCAGTATCTCCAAAAGTCCTTGGAAGCAGAT
CCTAATTCTGGCCAGTCCTGGTATTTCCTCGGAAGGTGCTATTCAAGTATTGGGAAAGTT
CAGGATGCCTTTATATCTTACAGGCAGTCTATTGATAAATCAGAAGCAAGTGCAGATACA
TGGTGTTCAATAGGTGTGCTATATCAGCAGCAAAATCAGCCCATGGATGCTTTACAGGCC
TATATTTGTGCTGTACAATTGGACCATGGCCATGCTGCAGCCTGGATGGACCTAGGCACT
CTCTATGAATCCTGCAACCAGCCTCAGGATGCCATTAAATGCTACTTAAATGCAACTAGA
AGCAAAAGTTGTAGTAATACCTCTGCACTTGCAGCACGAATTAAGTATTTACAGGCTCAG
TTGTGTAACCTTCCACAAGGTAGTCTACAGAATAAAACTAAATTACTTCCTAGTATTGAG
GAGGCGTGGAGCCTACCAATTCCCGCAGAGCTTACCTCCAGGCAGGGTGCCATGAACACA
GCACAGCAGGCATGTAAACCTCATCATCCAAATACTGAACCTGTATTAGGCCTCAGTCAA
ACACCAATTTCACAGCAATCCTTGCCACTACACATGATTCCTTCTAGCCAAGTAGATGAC
CTGTCCAGTCCTGCCAAGAGGAAAAGAACATCTAGTCCAACAAAGAATACTTCTGACAAT
TGGAGTGGTGGACATGCTGTGTCACATCCTCCAGTACAGCAACAAGCTCATTCATGGTGT
TTGACACCACAGAAATTACAGCATTTGGAACAGCTCCGCGCAAATAGAAATAATTTAAAT
CCAGCACAGAAACTGATGCTGGAACAGCTGGAAAGTCAGTTTGTCTTAATGCAACAACAC
CAAATGAGACCAACAGGAGTTGCACAGGTACGATCTACTGGAATTCCTAATGGGCCAACA
GCTGACTCATCACTGCCTACAAACTCAGTCTCTGGCCAGCAGCCACAGCTTGCTCTGACC
AGAGTGCCTAGCGTCTCTCAGCCTGGAGTCCGTCCTGCCTGCCCTGGGCAGCCTTTGGCC
AATGGACCCTTTTCTGCAGGCCATGTTCCCTGTAGCACATCAAGAACGCTGGGAAGTACA
GACACTATTTTGATAGGCAATAATCATATAACAGGAAGTGGAAGTAATGGAAACGTGCCT
TACCTGCAGCGAAACGCACTCACTCTACCTCATAACCGCACAAACCTGACCAGCAGCGCA
GAGGAGCCGTGGAAAAACCAACTATCTAACTCCACTCAGGGGCTTCACAAAGGTCAGAGT
TCACATTCGGCAGGTCCTAATGGTGAACGACCTCTCTCTTCCACTGGGCCTTCCCAGCAT
CTCCAGGCAGCTGGCTCTGGTATTCAGAATCAGAACGGACATCCCACCCTGCCTAGCAAT
TCAGTAACACAGGGGGCTGCTCTCAATCACCTCTCCTCTCACACTGCTACCTCAGGTGGA
CAACAAGGCATTACCTTAACCAAAGAGAGCAAGCCTTCAGGAAACATATTGACGGTGCCT
GAAACAAGCAGGCACACTGGAGAGACACCTAACAGCACTGCCAGTGTCGAGGGACTTCCT
AATCATGTCCATCAGATGACGGCAGATGCTGTTTGCAGTCCTAGCCATGGAGATTCTAAG
TCACCAGGTTTACTAAGTTCAGACAATCCTCAGCTCTCTGCCTTGTTGATGGGAAAAGCC
AATAACAATGTGGGTACTGGAACCTGTGACAAAGTCAATAACATCCACCCAGCTGTTCAT
ACAAAGACTGATAACTCTGTTGCCTCTTCACCATCTTCAGCCATTTCAACAGCAACACCT
TCTCCAAAATCCACTGAGCAGACAACCACAAACAGTGTTACCAGCCTTAACAGCCCTCAC
AGTGGGCTACACACAATTAATGGAGAAGGGATGGAAGAATCTCAGAGCCCCATGAAAACA
GATCTGCTTCTGGTTAACCACAAACCTAGTCCACAGATCATACCATCAATGTCTGTGTCC
ATATACCCCAGCTCAGCAGAAGTTCTGAAGGCATGCAGGAATCTAGGTAAAAATGGCTTA
TCTAACAGTAGCATTTTGTTGGATAAATGTCCACCTCCAAGACCACCATCTTCACCATAC
CCTCCCTTGCCAAAGGACAAGTTGAATCCACCTACACCTAGTATTTACTTGGAAAATAAA
CGTGATGCTTTCTTTCCTCCATTACATCAATTTTGTACAAATCCGAACAACCCTGTTACA
GTAATACGTGGCCTTGCTGGAGCTCTTAAGTTAGACCTGGGACTTTTCTCTACTAAAACT
TTGGTGGAAGCTAACAATGAACATATGGTAGAAGTGAGGACACAGTTGTTGCAGCCAGCA
GATGAAAACTGGGATCCCACTGGAACAAAGAAAATCTGGCATTGTGAAAGTAATAGATCT
CATACTACAATTGCTAAATATGCACAGTACCAGGCCTCCTCATTCCAGGAATCATTGAGA
GAAGAAAATGAAAAAAGAAGTCATCATAAAGACCACTCAGATAGTGAATCTACATCGTCA
GATAATTCTGGGAGGAGGAGGAAAGGACCCTTTAAAACCATAAAGTTTGGGACCAATATT
GACCTATCTGATGACAAAAAGTGGAAGTTGCAGCTACATGAGCTGACTAAACTTCCTGCT
TTTGTGCGTGTCGTATCAGCAGGAAATCTTCTAAGCCATGTTGGTCATACCATATTGGGC
ATGAACACAGTTCAACTATACATGAAAGTTCCAGGGAGCAGAACACCAGGTCATCAGGAA
AATAACAACTTCTGTTCAGTTAACATAAATATTGGCCCAGGTGACTGTGAATGGTTTGTT
GTTCCTGAAGGTTACTGGGGTGTTCTGAATGACTTCTGTGAAAAAAATAATTTGAATTTC
CTAATGGGTTCTTGGTGGCCCAATCTTGAAGATCTTTATGAAGCAAATGTTCCAGTGTAT
AGGTTTATTCAGCGACCTGGAGATTTGGTCTGGATAAATGCAGGCACTGTTCATTGGGTT
CAGGCTATTGGCTGGTGCAACAACATTGCTTGGAATGTTGGTCCACTTACAGCCTGCCAG
TATAAATTGGCAGTGGAACGGTACGAATGGAACAAATTGCAAAGTGTGAAGTCAATAGTA
CCCATGGTTCATCTTTCCTGGAATATGGCACGAAATATCAAGGTCTCAGATCCAAAGCTT
TTTGAAATGATTAAGTATTGTCTTCTAAGAACTCTGAAGCAATGTCAGACATTGAGGGAA
GCTCTCATTGCTGCAGGAAAAGAGATTATATGGCATGGGCGGACAAAAGAAGAACCAGCT
CATTACTGTAGCATTTGTGAAGTGGAGGTTTTTGATCTGCTTTTTGTCACTAATGAGAGT
AATTCACGAAAGACCTACATAGTACATTGCCAAGATTGTGCACGAAAAACAAGCGGAAAC
TTGGAAAACTTTGTGGTGCTAGAACAGTACAAAATGGAGGACCTGATGCAAGTCTATGAC
CAATTTACATTAGCTCCTCCATTACCATCCGCCTCATCTTTG
Protein sequence predicted based on nucleotide sequence
MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFV RFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYY SLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVN TDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQ VKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKV QDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGT LYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIE EAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDD LSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLN PAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALT RVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVP YLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQH LQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVP ETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKA NNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPH SGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGL SNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVT VIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRS HTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNI DLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQE NNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVY RFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIV PMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPA HYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYD QFTLAPPLPSASSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
