Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860598
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3657nts
Open reading frame: 1 to 3657
ATGGCATTTACAGAAAGAGTCAACAGCAGTGGCAACAGTTTGTACAATGACGACAGAAAC
CTGCTTCGAATTAGAGAGAAGGAAAGACGCAACCAGGAAGCCCACCAAGAGAAAGAGGCA
TTTCCTGAAAAGATTCCCCTTTTTGGAGAGCCCTACAAGACAGCAAAAGGTGATGAGCTG
TCTAGTCGAATACAGAACATGTTGGGAAACTACGAAGAAGTGAAGGAGTTCCTTAGTACT
AAGTCTCACACTCATCGCCTGGATGCTTCTGAAAATAGGTTGGGAAAGCCGAAATATCCT
TTAATTCCTGACAAAGGGAGCAGCATTCCATCCAGCTCCTTCCACACTAGTGTCCACCAC
CAGTCCATTCACACTCCTGCGTCTGGACCACTTTCTGTTGGCAACATTAGCCACAATCCA
AAGATGGCGCAGCCAAGAACTGAACCAATGCCAAGTCTCCATGCCAAAAGCTGCGGCCCA
CCGGACAGCCAGCACCTGACCCAGGATCGCCTTGGTCAGGAGGGGTTCGGCTCTAGTCAT
CACAAGAAAGGTGACCGAAGAGCTGACGGAGACCACTGTGCTTCGGTGACAGATTCGGCT
CCAGAGAGGGAGCTTTCTCCCTTAATCTCTTTGCCTTCCCCAGTTCCCCCTTTGTCACCT
ATACATTCCAACCAGCAAACTCTTCCCCGGACGCAAGGAAGCAGCAAGGTTCATGGCAGC
AGCAATAACAGTAAAGGCTATTGCCCAGCCAAATCTCCCAAGGACCTAGCAGTGAAAGTC
CATGATAAAGAGACCCCTCAAGACAGTTTGGTGGCCCCTGCCCAGCCGCCTTCTCAGACA
TTTCCACCTCCCTCCCTCCCCTCAAAAAGTGTTGCAATGCAGCAGAAGCCCACGGCTTAT
GTCCGGCCCATGGATGGTCAAGATCAGGCCCCTAGTGAATCCCCTGAACTGAAACCACTG
CCGGAGGACTATCGACAGCAGACCTTTGAAAAAACAGACTTGAAAGTGCCTGCCAAAGCC
AAGCTCACCAAACTGAAGATGCCTTCTCAGTCAGTTGAGCAGACCTACTCCAATGAAGTC
CATTGTGTTGAAGAGATTCTGAAGGAAATGACCCATTCATGGCCGCCTCCTTTGACAGCA
ATACATACGCCTAGTACAGCTGAGCCATCCAAGTTTCCTTTCCCTACAAAGGACTCTCAG
CATGTCAGTTCTGTAACCCAAAACCAAAAACAATATGATACATCTTCAAAAACTCACTCA
AATTCTCAGCAAGGAACGTCATCCATGCTCGAAGACGACCTTCAGCTCAGTGACAGTGAG
GACAGTGACAGTGAACAAACCCCAGAGAAGCCTCCCTCCTCATCTGCACCTCCAAGTGCT
CCACAGTCCCTTCCAGAACCAGTGGCATCAGCACATTCCAGCAGTGCAGAGTCAGAAAGC
ACCAGTGACTCAGACAGTTCCTCAGACTCAGAGAGCGAGAGCAGTTCAAGTGACAGCGAA
GAAAATGAGCCCCTAGAAACCCCAGCTCCGGAGCCTGAGCCTCCAACAACAAACAAATGG
CAGCTGGACAACTGGCTGACCAAAGTCAGCCAGCCAGCTGCGCCACCAGAGGGCCCCAGG
AGCACAGAGCCCCCACGGCGGCACCCAGAGAGTAAGGGCAGCAGCGACAGTGCCACGAGT
CAGGAGCATTCTGAATCCAAAGATCCTCCCCCTAAAAGCTCCAGCAAAGCCCCCCGGGCC
CCACCCGAAGCCCCCCACCCCGGAAAGAGGAGCTGTCAGAAGTCTCCGGCACAGCAGGAG
CCCCCACAAAGGCAAACCGTTGGAACCAAACAACCCAAAAAACCTGTCAAGGCCTCTGCC
CGGGCAGGTTCACGGACCAGCCTGCAGGGGGAAAGGGAGCCAGGGCTTCTTCCCTATGGC
TCCCGAGACCAGACTTCCAAAGACAAGCCCAAGGTGAAGACGAAAGGACGGCCCCGGGCC
GCAGCAAGCAACGAACCCAAGCCAGCAGTGCCCCCCTCCAGTGAGAAGAAGAAGCACAAG
AGCTCCCTCCCTGCCCCCTCTAAGGCTCTCTCAGGCCCAGAACCCGCGAAGGACAATGTG
GAGGACAGGACCCCTGAGCACTTTGCTCTTGTTCCCCTGACTGAGAGCCAGGGCCCACCC
CACAGTGGCAGCGGCAGCAGGACTAGTGGCTGCCGCCAAGCCGTGGTGGTCCAGGAGGAC
AGCCGCAAAGACAGACTCCCATTGCCTTTGAGAGACACCAAGCTGCTCTCACCGCTCAGG
GACACTCCTCCCCCACAAAGCTTGATGGTGAAGATCACCCTAGACCTGCTCTCTCGGATA
CCCCAGCCTCCCGGGAAGGGGAGCCGCCAGAGGAAAGCAGAAGATAAACAGCCGCCCGCA
GGGAAGAAGCACAGCTCTGAGAAGAGGAGCTCAGACAGCTCAAGCAAGTTGGCCAAAAAG
AGAAAGGGTGAAGCAGAAAGAGACTGTGATAACAAGAAAATCAGACTGGAGAAGGAAATC
AAATCACAGTCATCTTCATCTTCATCCTCCCACAAAGAATCTTCTAAAACAAAGCCCTCC
AGGCCCTCCTCACAGTCCTCAAAGAAGGAAATGCTCCCCCCGCCACCCGTGTCCTCGTCC
TCCCAGAAGCCAGCCAAGCCTGCACTTAAGAGGTCAAGGCGGGAAGCAGACACCTGTGGC
CAGGACCCTCCCAAAAGTGCCAGCAGTACCAAGAGCAACCACAAAGACTCTTCCATTCCC
AAGCAGAGAAGAGTAGAGGGGAAGGGCTCCAGAAGCTCCTCGGAGCACAAGGGTTCTTCC
GGAGATACTGCAAATCCTTTTCCAGTGCCTTCTTTGCCAAATGGTAACTCTAAACCAGGG
AAGCCTCAAGTGAAGTTTGACAAACAACAAGCAGACCTTCACATGAGGGAGGCAAAAAAG
ATGAAGCAGAAAGCAGAGTTAATGACGGACAGGGTTGGAAAGGCTTTTAAGTACCTGGAA
GCCGTCTTGTCCTTCATTGAGTGCGGAATTGCCACAGAGTCTGAAAGCCAGTCATCCAAG
TCAGCTTACTCTGTCTACTCAGAAACTGTAGATCTCATTAAATTCATAATGTCATTAAAA
TCCTTCTCAGATGCCACAGCGCCAACACAAGAGAAAATATTTGCTGTTTTATGCATGCGT
TGCCAGTCCATTTTGAACATGGCGATGTTTCGTTGTAAAAAAGACATAGCAATAAAGTAT
TCTCGTACTCTTAATAAACACTTCGAGAGTTCTTCCAAAGTCGCCCAGGCACCTTCTCCA
TGCATTGCAAGAAGCACAGGCACACCATCCCCTCTTTCCCCAATGCCTTCTCCTGCCAGC
TCCGTAGGGTCCCAGTCAAGTGCTGGCAGTGTGGGGAGCAGTGGGGTGGCTGCCACTATC
AGCACCCCAGTCACCATCCAGAATATGACATCTTCCTATGTCACCATCACATCCCATGTT
CTTACCGCCTTTGACCTTTGGGAACAGGCCGAGGCCCTCACGAGGAAGAATAAAGAATTC
TTTGCTCGGCTCAGCACAAATGTGTGCACCTTGGCCCTCAACAGCAGTTTGGTGGACCTG
GTGCACTATACACGACAGGGTTTTCAGCAGCTACAAGAATTAACCAAAACACCTTTG
Protein sequence predicted based on nucleotide sequence
MAFTERVNSSGNSLYNDDRNLLRIREKERRNQEAHQEKEAFPEKIPLFGEPYKTAKGDEL SSRIQNMLGNYEEVKEFLSTKSHTHRLDASENRLGKPKYPLIPDKGSSIPSSSFHTSVHH QSIHTPASGPLSVGNISHNPKMAQPRTEPMPSLHAKSCGPPDSQHLTQDRLGQEGFGSSH HKKGDRRADGDHCASVTDSAPERELSPLISLPSPVPPLSPIHSNQQTLPRTQGSSKVHGS SNNSKGYCPAKSPKDLAVKVHDKETPQDSLVAPAQPPSQTFPPPSLPSKSVAMQQKPTAY VRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSVEQTYSNEV HCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHVSSVTQNQKQYDTSSKTHS NSQQGTSSMLEDDLQLSDSEDSDSEQTPEKPPSSSAPPSAPQSLPEPVASAHSSSAESES TSDSDSSSDSESESSSSDSEENEPLETPAPEPEPPTTNKWQLDNWLTKVSQPAAPPEGPR STEPPRRHPESKGSSDSATSQEHSESKDPPPKSSSKAPRAPPEAPHPGKRSCQKSPAQQE PPQRQTVGTKQPKKPVKASARAGSRTSLQGEREPGLLPYGSRDQTSKDKPKVKTKGRPRA AASNEPKPAVPPSSEKKKHKSSLPAPSKALSGPEPAKDNVEDRTPEHFALVPLTESQGPP HSGSGSRTSGCRQAVVVQEDSRKDRLPLPLRDTKLLSPLRDTPPPQSLMVKITLDLLSRI PQPPGKGSRQRKAEDKQPPAGKKHSSEKRSSDSSSKLAKKRKGEAERDCDNKKIRLEKEI KSQSSSSSSSHKESSKTKPSRPSSQSSKKEMLPPPPVSSSSQKPAKPALKRSRREADTCG QDPPKSASSTKSNHKDSSIPKQRRVEGKGSRSSSEHKGSSGDTANPFPVPSLPNGNSKPG KPQVKFDKQQADLHMREAKKMKQKAELMTDRVGKAFKYLEAVLSFIECGIATESESQSSK SAYSVYSETVDLIKFIMSLKSFSDATAPTQEKIFAVLCMRCQSILNMAMFRCKKDIAIKY SRTLNKHFESSSKVAQAPSPCIARSTGTPSPLSPMPSPASSVGSQSSAGSVGSSGVAATI STPVTIQNMTSSYVTITSHVLTAFDLWEQAEALTRKNKEFFARLSTNVCTLALNSSLVDL VHYTRQGFQQLQELTKTPL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
