Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860908
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5316nts
Open reading frame: 1 to 5316
ATGTCAGTTTTGATATCACAGAGCGTCATAAATTATGTAGAGGAAGAAAACATTCCTGCT
CTGAAAGCTCTTCTTGAAAAATGCAAAGATGTAGATGAGAGAAATGAGTGTGGCCAGACT
CCACTGATGATAGCTGCCGAACAAGGCAATCTGGAAATAGTGAAGGAATTAATTAAGAAT
GGAGCTAACTGCAATCTGGAAGATTTGGATAATTGGACAGCACTTATATCTGCATCGAAA
GAAGGGCATGTGCACATCGTAGAGGAACTACTGAAATGTGGGGTTAACTTGGAGCACCGT
GATATGGGAGGATGGACAGCTCTTATGTGGGCATGTTACAAAGGCCGTACTGACGTAGTA
GAGTTGCTTCTTTCTCATGGTGCCAATCCAAGTGTCACTGGTCTGTACAGTGTTTACCCA
ATCATTTGGGCAGCAGGGAGAGGCCATGCAGATATAGTTCATCTTTTACTGCAAAATGGT
GCTAAAGTCAACTGCTCTGATAAGTATGGAACCACCCCTTTAGTTTGGGCTGCACGAAAG
GGTCATTTGGAATGTGTGAAACATTTATTGGCCATGGGAGCTGATGTGGATCAAGAAGGA
GCTAATTCAATGACTGCACTTATTGTGGCAGTGAAAGGAGGTTACACACAGTCAGTAAAA
GAAATTTTGAAGAGGAATCCAAATGTAAACTTAACAGATAAAGATGGAAATACAGCTTTG
ATGATTGCATCAAAGGAGGGACATACGGAGATTGTGCAGGATCTGCTCGACGCTGGAACA
TATGTGAACATACCTGACAGGAGTGGGGATACTGTGTTGATTGGCGCTGTCAGAGGTGGT
CATGTTGAAATTGTTCGAGCGCTTCTCCAAAAATATGCTGATATAGACATTAGAGGACAG
GATAATAAAACTGCTTTGTATTGGGCTGTTGAGAAAGGAAATGCAACAATGGTGAGAGAT
ATCTTACAGTGCAATCCTGACACTGAAATATGCACAAAGGATGGTGAAACGCCACTTATA
AAGGCTACCAAGATGAGAAACATTGAAGTGGTGGAGCTGCTGCTAGATAAAGGTGCTAAA
GTGTCTGCTGTAGATAAGAAAGGAGATACTCCCTTGCATATTGCTATTCGTGGAAGGAGC
CGGAAACTGGCAGAACTGCTTTTAAGAAATCCCAAAGATGGGCGATTACTTTATAGGCCC
AACAAAGCAGGCGAGACTCCTTATAATATTGACTGTAGCCATCAGAAGAGTATTTTAACT
CAAATATTTGGAGCCAGACACTTGTCTCCTACTGAAACAGACGGTGACATGCTTGGATAT
GATTTATATAGCAGTGCCCTGGCAGATATTCTCAGTGAGCCTACCATGCAGCCACCCATT
TGTGTGGGGTTATATGCACAGTGGGGAAGTGGGAAATCTTTCTTACTCAAGAAACTAGAA
GACGAAATGAAAACCTTCGCCGGACAACAGATTGAGCCTCTCTTTCAGTTCTCATGGCTC
ATAGTGTTTCTTACCCTGCTACTTTGTGGAGGGCTTGGTTTATTGTTTGCCTTCACGGTC
CACCCAAATCTTGGAATAGCAGTGTCACTGAGCTTCTTGGCTCTCTTATATATATTCTTT
ATTGTCATTTACTTTGGTGGACGAAGAGAAGGAGAGAGTTGGAATTGGGCCTGGGTCCTC
AGCACTAGATTGGCAAGACATATTGGATATTTGGAACTCCTCCTTAAATTGATGTTTGTG
AATCCACCTGAGTTGCCAGAGCAGACTACTAAAGCTTTACCTGTGAGGTTTTTGTTTACA
GATTACAATAGACTGTCCAGTGTAGGTGGAGAAACTTCTCTGGCTGAAATGATTGCAACC
CTCTCGGATGCTTGTGAAAGAGAGTTTGGCTTTTTGGCAACCAGGCTTTTTCGAGTATTC
AAGACTGAAGATACTCAGGGTAAAAAGAAATGGAAAAAAACATGTTGTCTCCCATCTTTT
GTCATCTTCCTTTTTATCATTGGCTGCATTATATCTGGAATTACTCTTCTGGCTATATTT
AGAGTTGACCCAAAGCATCTGACTGTAAATGCTGTCCTCATATCAATCGCATCTGTAGTG
GGATTGGCCTTTGTGTTGAACTGTCGTACATGGTGGCAAGTGCTGGACTCGCTCCTGAAT
TCCCAAAGAAAACGCCTCCATAATGCAGCCTCCAAACTGCACAAATTGAAAAGTGAAGGA
TTCATGAAAGTTCTTAAATGTGAAGTGGAATTGATGGCCAGGATGGCAAAAACCATTGAC
AGCTTCACTCAGAATCAGACAAGGCTGGTGGTCATCATCGATGGATTAGATGCCTGTGAG
CAGGACAAAGTCCTTCAGATGCTGGACACTGTCCGAGTTCTGTTTTCAAAAGGCCCGTTC
ATTGCCATTTTTGCAAGTGATCCACATATTATCATAAAGGCAATTAACCAGAACCTCAAT
AGTGTGCTTCGGGATTCAAATATAAATGGCCATGACTACATGCGCAACATAGTCCACTTG
CCTGTGTTCCTTAATAGTCGTGGACTAAGCAATGCAAGAAAATTTCTCGTAACTTCAGCA
ACAAATGGAGACGTTCCATGCTCAGATACTACAGGGATACAGGAAGATGCTGACAGAAGA
GTTTCACAGAACAGCCTTGGGGAGATGACAAAACTTGGTAGCAAGACAGCCCTCAATAGA
CGGGACACTTACCGAAGAAGGCAGATGCAGAGGACCATCACTCGCCAGATGTCCTTTGAT
CTTACAAAACTGCTGGTTACCGAGGACTGGTTCAGTGACATCAGTCCCCAGACCATGAGA
AGATTACTTAATATTGTTTCTGTGACAGGACGATTACTGAGAGCCAATCAGATTAGTTTC
AACTGGGACAGGCTTGCTAGCTGGATCAACCTTACTGAGCAGTGGCCATACCGGACTTCA
TGGCTCATATTATATTTGGAAGAGACTGAAGGTATTCCAGATCAAATGACATTAAAAACC
ATCTACGAAAGAATATCAAAGAATATTCCAACAACTAAGGATGTTGAGCCACTTCTTGAA
ATTGATGGAGATATAAGAAATTTTGAAGTGTTTTTGTCTTCAAGGACCCCAGTTCTTGTG
GCTCGAGATGTAAAAGTCTTTTTGCCATGCACTGTAAACCTAGATCCCAAACTACGGGAA
ATTATTGCAGATGTTCGTGCTGCCAGAGAGCAGATCAGTATTGGAGGACTGGCGTACCCC
CCGCTCCCTCTACATGAGGGTCCTCCTAGGGCGCCATCAGGGTACAGCCAGCCCCCATCC
GTGTGCTCTTCCACGTCCTTCAATGGGCCCTTCGCAGGTGGAGTGGTGTCACCACAGCCT
CACAGCAGCTATTACAGCGGCATGACGGGCCCTCAGCATCCCTTCTACAACAGGCCATTC
TTTGCCCCATACCTTTACACGCCAAGGTATTACCCTGGCGGCTCCCAACATCTCATCTCA
CGTCCATCAGTAAAAACGAGTTTGCCCAGAGATCAGAACAATGGCCTAGAAGTTATCAAG
GAGGATGCTGCTGAGGGGCTTTCTTCACCCACAGACTCCTCGAGGGGGTCAGGCCCAGCC
CCAGGCCCAGTGGTATTACTGAATTCACTGAATGTGGATGCAGTATGTGAGAAGCTGAAA
CAAATAGAAGGGCTGGACCAGAGTATGCTGCCTCAGTATTGTACCACGATCAAAAAGGCA
AACATAAATGGCCGTGTGTTAGCTCAGTGTAACATTGATGAGCTGAAGAAAGAGATGAAT
ATGAATTTTGGAGACTGGCACCTTTTCAGAAGCACAGTACTAGAAATGAGAAACGCAGAA
AGCCACGTGGTCCCTGAAGACCCACGTTTCCTCAGTGAGAGCAGCAGTGGCCCAGCCCCG
CACGGTGAGCCTGCTCGCCGCGCTTCCCACAACGAGCTGCCTCACACCGAGCTCTCCAGC
CAGACGCCCTACACACTCAACTTCAGCTTCGAAGAGCTGAACACGCTTGGCCTGGATGAA
GGTGCCCCTCGTCACAGTAATCTAAGTTGGCAGTCACAAACTCGCAGAACCCCAAGTCTT
TCGAGTCTCAATTCCCAGGATTCCAGTATTGAAATTTCAAAGCTTACTGATAAGGTGCAG
GCCGAGTATAGAGATGCCTATAGAGAATACATTGCTCAGATGTCCCAGTTAGAAGGGGGC
CCCGGGTCTACAACCATTAGTGGCAGATCTTCTCCACATAGCACATATTACATGGGTCAG
AGTTCATCAGGGGGCTCTATTCATTCAAACCTAGAGCAAGAAAAGGGGAAGGATAGTGAA
CCAAAGCCCGATGATGGGAGGAAGTCCTTTCTAATGAAGAGGGGAGATGTTATCGATTAT
TCATCATCAGGGGTTTCCACCAACGATGCTTCCCCCCTGGATCCTATCACTGAAGAAGAT
GAAAAATCAGATCAGTCAGGCAGTAAGCTTCTCCCAGGCAAGAAATCTTCCGAAAGGTCA
AGCCTCTTCCAGACAGATTTGAAGCTTAAGGGAAGTGGGCTGCGCTATCAAAAACTCCCA
AGTGACGAGGATGAATCTGGCACAGAAGAATCAGATAACACTCCACTGCTCAAAGATGAC
AAAGACAGAAAAGCCGAAGGGAAAGTAGAGAGAGTGCCGAAGTCTCCAGAACACAGTGCT
GAGCCGATCAGAACCTTCATTAAAGCCAAAGAGTATTTATCGGATGCGCTCCTTGACAAA
AAGGATTCATCGGATTCAGGAGTGAGATCCAGTGAAAGTTCTCCCAATCACTCTCTGCAC
AATGAAGTGGCGGATGACTCCCAGCTTGAAAAGGCAAATCTCATAGAGCTGGAAGATGAC
AGTCACAGCGGAAAGCGGGGAATCCCACATAGCCTGAGTGGCCTGCAAGATCCAATTATA
GCTCGGATGTCCATTTGTTCAGAAGACAAGAAAAGCCCTTCCGAATGCAGCTTGATAGCC
AGCAGCCCTGAAGAAAACTGGCCTGCATGCCAGAAAGCCTACAACCTGAACCGAACTCCC
AGCACCGTGACTCTGAACAACAATAGTGCTCCAGCCAACAGAGCCAATCAAAATTTCGAT
GAGATGGAGGGAATTAGGGAGACTTCTCAAGTCATTTTGAGGCCTAGTTCCAGTCCCAAC
CCAACCACTATTCAGAATGAGAATCTAAAAAGCATGACACATAAGCGAAGCCAACGTTCA
AGTTACACAAGGCTCTCCAAAGATCCTCCGGAGCTCCATGCAGCAGCCTCTTCTGAGAGC
ACAGGCTTTGGAGAAGAAAGAGAAAGCATTCTTTTG
Protein sequence predicted based on nucleotide sequence
MSVLISQSVINYVEEENIPALKALLEKCKDVDERNECGQTPLMIAAEQGNLEIVKELIKN GANCNLEDLDNWTALISASKEGHVHIVEELLKCGVNLEHRDMGGWTALMWACYKGRTDVV ELLLSHGANPSVTGLYSVYPIIWAAGRGHADIVHLLLQNGAKVNCSDKYGTTPLVWAARK GHLECVKHLLAMGADVDQEGANSMTALIVAVKGGYTQSVKEILKRNPNVNLTDKDGNTAL MIASKEGHTEIVQDLLDAGTYVNIPDRSGDTVLIGAVRGGHVEIVRALLQKYADIDIRGQ DNKTALYWAVEKGNATMVRDILQCNPDTEICTKDGETPLIKATKMRNIEVVELLLDKGAK VSAVDKKGDTPLHIAIRGRSRKLAELLLRNPKDGRLLYRPNKAGETPYNIDCSHQKSILT QIFGARHLSPTETDGDMLGYDLYSSALADILSEPTMQPPICVGLYAQWGSGKSFLLKKLE DEMKTFAGQQIEPLFQFSWLIVFLTLLLCGGLGLLFAFTVHPNLGIAVSLSFLALLYIFF IVIYFGGRREGESWNWAWVLSTRLARHIGYLELLLKLMFVNPPELPEQTTKALPVRFLFT DYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFKTEDTQGKKKWKKTCCLPSF VIFLFIIGCIISGITLLAIFRVDPKHLTVNAVLISIASVVGLAFVLNCRTWWQVLDSLLN SQRKRLHNAASKLHKLKSEGFMKVLKCEVELMARMAKTIDSFTQNQTRLVVIIDGLDACE QDKVLQMLDTVRVLFSKGPFIAIFASDPHIIIKAINQNLNSVLRDSNINGHDYMRNIVHL PVFLNSRGLSNARKFLVTSATNGDVPCSDTTGIQEDADRRVSQNSLGEMTKLGSKTALNR RDTYRRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISF NWDRLASWINLTEQWPYRTSWLILYLEETEGIPDQMTLKTIYERISKNIPTTKDVEPLLE IDGDIRNFEVFLSSRTPVLVARDVKVFLPCTVNLDPKLREIIADVRAAREQISIGGLAYP PLPLHEGPPRAPSGYSQPPSVCSSTSFNGPFAGGVVSPQPHSSYYSGMTGPQHPFYNRPF FAPYLYTPRYYPGGSQHLISRPSVKTSLPRDQNNGLEVIKEDAAEGLSSPTDSSRGSGPA PGPVVLLNSLNVDAVCEKLKQIEGLDQSMLPQYCTTIKKANINGRVLAQCNIDELKKEMN MNFGDWHLFRSTVLEMRNAESHVVPEDPRFLSESSSGPAPHGEPARRASHNELPHTELSS QTPYTLNFSFEELNTLGLDEGAPRHSNLSWQSQTRRTPSLSSLNSQDSSIEISKLTDKVQ AEYRDAYREYIAQMSQLEGGPGSTTISGRSSPHSTYYMGQSSSGGSIHSNLEQEKGKDSE PKPDDGRKSFLMKRGDVIDYSSSGVSTNDASPLDPITEEDEKSDQSGSKLLPGKKSSERS SLFQTDLKLKGSGLRYQKLPSDEDESGTEESDNTPLLKDDKDRKAEGKVERVPKSPEHSA EPIRTFIKAKEYLSDALLDKKDSSDSGVRSSESSPNHSLHNEVADDSQLEKANLIELEDD SHSGKRGIPHSLSGLQDPIIARMSICSEDKKSPSECSLIASSPEENWPACQKAYNLNRTP STVTLNNNSAPANRANQNFDEMEGIRETSQVILRPSSSPNPTTIQNENLKSMTHKRSQRS SYTRLSKDPPELHAAASSESTGFGEERESILL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
