Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00861271
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5691nts
Open reading frame: 1 to 5691
ATGCCGCTGCCACCGCGGAGCCTGCAGGTGCTCCTGCTGCTGCTGCTGTTGCTGCTGCTG
CTGCCGGGCATGTGGGCTGAGGCAGGCTTGCCCAGGGCAGGCGGGGGTTCACAGCCCCCC
TTCCGCACCTTCTCGGCCAGCGACTGGGGCCTCACCCACCTAGTGGTGCATGAGCAGACA
GGCGAGGTGTATGTGGGCGCAGTGAACCGCATCTATAAGCTGTCGGGGAACCTGACACTG
CTGCGGGCCCACGTCACGGGCCCTGTGGAGGACAACGAGAAGTGCTACCCGCCGCCCAGC
GTGCAGTCCTGCCCCCACGGCCTGGGCAGTACTGACAACGTCAACAAGCTGCTGCTGCTG
GACTATGCCGCTAACCGCCTGCTGGCCTGTGGCAGCGCCTCCCAGGGCATCTGCCAGTTC
CTGCGTCTGGACGATCTCTTCAAACTGGGTGAGCCACACCACCGTAAGGAGCACTACCTG
TCCAGCGTGCAGGAGGCAGGCAGCATGGCGGGCGTGCTCATTGCCGGGCCACCGGGCCAG
GGCCAGGCCAAGCTCTTCGTGGGCACACCCATCGATGGCAAGTCCGAGTACTTCCCCACA
CTGTCCAGCCGTCGGCTCATGGCCAACGAGGAGGATGCCGACATGTTCGGCTTCGTGTAC
CAGGATGAGTTTGTGTCATCACAGCTCAAGATCCCTTCGGACACGCTGTCCAAGTTCCCG
GCCTTTGACATCTACTATGTGTACAGCTTCCGCAGCGAGCAGTTTGTCTACTACCTCACG
CTGCAGCTAGACACACAGCTGACCTCGCCTGATGCCGCCGGCGAGCACTTCTTCACGTCC
AAGATCGTGCGGCTCTGTGTGGACGACCCCAAATTCTACTCGTACGTTGAGTTCCCCATT
GGCTGCGAGCAGGCGGGTGTGGAGTACCGCCTGGTGCAGGATGCCTACCTGAGCCGGCCC
GGCCGTGCCCTGGCCCACCAGCTGGGCCTGGCTGAGGACGAGGACGTGCTGTTCACTGTG
TTCGCCCAGGGCCAGAAGAACCGCGTGAAGCCACCAAAGGAGTCAGCACTGTGCCTGTTC
ACGCTCAGGGCCATCAAGGAGAAGATTAAGGAGCGCATCCAGTCCTGCTACCGTGGTGAG
GGCAAGCTCTCCCTGCCGTGGCTGCTCAACAAGGAGCTGGGCTGCATCAACTCGCCCCTG
CAGATCGATGACGACTTCTGCGGGCAGGACTTCAACCAGCCCCTGGGGGGCACAGTCACC
ATTGAGGGGACGCCCCTGTTCGTGGACAAGGATGATGGCCTGACCGCCGTGGCTGCCTAT
GACTATCGGGGCCGCACTGTGGTATTCGCCGGCACGCGAAGTGGCCGCATCCGCAAGATC
CTGGTGGACCTCTCAAACCCCGGTGGCCGGCCTGCCCTGGCCTACGAGAGCGTCGTGGCC
CAGGAGGGCAGCCCCATCCTGCGAGACCTCGTCCTCAGCCCCAACCACCAGTACCTCTAC
GCCATGACCGAGAAGCAGGTGACGCGGGTGCCTGTGGAGAGCTGTGTGCAGTACACGTCC
TGTGAGCTGTGTCTGGGGTCACGGGACCCCCACTGTGGCTGGTGTGTCCTGCACAGCATC
TGCTCGCGGCGGGACGCCTGTGAGCGAGCAGACGAGCCCCAGCGCTTTGCTGCGGACCTG
CTGCAGTGTGTGCAGCTGACTGTGCAGCCCCGCAATGTGTCTGTCACCATGTCCCAGGTC
CCACTTGTGCTGCAGGCCTGGAACGTGCCTGACCTCTCAGCTGGCGTCAACTGCTCCTTC
GAGGACTTCACGGAATCTGAGAGCGTCCTGGAGGATGGCCGGATCCACTGCCGCTCACCC
TCCGCCCGGGAGGTGGCGCCCATCACGCGGGGCCAGGGAGACCAGCGGGTGGTGAAACTC
TACCTAAAGTCCAAGGAGACAGGGAAGAAGTTTGCGTCTGTGGACTTCGTCTTCTACAAC
TGCAGCGTCCACCAGTCCTGCCTGTCCTGTGTCAACGGCTCCTTTCCCTGCCACTGGTGC
AAATACCGCCACGTGTGCACACACAACGTGGCTGACTGCGCCTTCCTGGAGGGCCGTGTC
AACGTGTCTGAGGACTGCCCACAGATCCTGCCCTCCACGCAGATCTACGTGCCAGTGGGA
GTGGTAAAACCCATCACCCTGGCCGCACGGAACCTGCCACAGCCACAGTCAGGCCAGCGT
GGATATGAGTGCCTCTTCCACATCCCGGGCAGCCCGGCCCGTGTCACCGCCCTGCGCTTC
AACAGCTCCAGCCTGCAGTGCCAGAATTCCTCGTACTCCTACGAGGGGAACGATGTCAGC
GACCTGCCAGTGAACCTGTCAGTCGTGTGGAACGGCAACTTTGTCATTGACAACCCACAG
AACATCCAGGCGCACCTCTACAAGTGCCCGGCCCTGCGCGAGAGCTGCGGCCTCTGCCTC
AAGGCCGACCCGCGCTTCGAGTGCGGATGGTGCGTGGCCGAGCGCCGCTGCTCCCTGCGA
CACCACTGCGCTGCCGACACACCTGCATCGTGGATGCACGCGCGTCACGGCAGCAGTCGC
TGCACCGACCCCAAGATCCTCAAGCTGTCCCCCGAGACGGGCCCGAGGCAGGGCGGCACG
CGGCTCACTATCACAGGCGAGAACCTGGGCCTGCGATTCGAAGACGTGCGTCTGGGCGTG
CGCGTGGGCAAGGTGCTGTGCAGCCCTGTGGAGAGCGAGTACATCAGTGCGGAGCAGATC
GTCTGTGAGATCGGGGACGCCAGCTCCGTGCGTGCCCATGACGCCCTGGTGGAGGTGTGT
GTGCGGGACTGCTCACCACACTACCGCGCCCTGTCACCCAAGCGCTTCACCTTCGTGACA
CCAACCTTCTACCGTGTGAGCCCCTCCCGTGGGCCTCTGTCAGGGGGCACCTGGATTGGC
ATCGAGGGAAGCCACCTGAACGCAGGCAGTGATGTGGCTGTGTCGGTCGGTGGCCGGCCC
TGCTCCTTCTCCTGGAGGAACTCCCGTGAGATCCGGTGCCTGACACCCCCCGGGCAGAGC
CCTGGCAGCGCTCCCATCATCATCAACATCAACCGCGCCCAGCTCACCAACCCTGAGGTG
AAGTACAACTACACCGAGGACCCCACCATCCTGAGGATCGACCCCGAGTGGAGCATCAAC
AGCGGTGGGACCCTCCTGACGGTCACAGGCACCAACCTGGCCACTGTCCGTGAACCCCGA
ATCCGGGCCAAGTATGGAGGCATTGAGAGGGAGAACGGCTGCCTGGTGTACAATGACACC
ACCATGGTATGCCGCGCCCCGTCTGTGGCCAACCCTGTGCGCAGCCCACCAGAGCTGGGG
GAGCGGCCGGATGAGCTGGGCTTCGTCATGGACAACGTGCGCTCCCTGCTTGTGCTCAAC
TCCACCTCCTTCCTCTACTACCCTGACCCCGTACTGGAGCCACTCAGCCCCACTGGCCTG
CTGGAGCTGAAGCCCAGCTCCCCACTCATCCTCAAGGGCCGGAACCTCTTGCCACCTGCA
CCCGGCAACTCCCGACTCAACTACACGGTGCTCATCGGCTCCACACCCTGTACCCTCACC
GTGTCGGAGACGCAACTGCTGTGCGAGGCGCCCAACCTCACTGGGCAGCACAAGGTCACG
GTGCGGGCAGGTGGCTTCGAGTTCTCGCCAGGGACACTGCAGGTGTACTCGGACAGCCTG
CTGACGCTGCCTGCCATTGTGGGCATTGGCGGAGGCGGGGGTCTCCTGCTGCTGGTCATC
GTGGCTGTGCTCATCGCCTACAAGCGCAAGTCACGAGATGCTGACCGCACACTCAAGCGG
CTGCAGCTCCAGATGGACAACCTGGAGTCCCGCGTGGCCCTCGAATGCAAGGAAGCCTTT
GCAGAGCTGCAGACAGACATCCACGAGCTGACCAATGACCTGGACGGTGCCGGCATCCCC
TTCCTTGACTACCGGACATATGCCATGCGGGTGCTCTTTCCTGGGATCGAGGACCACCCT
GTGCTCAAGGAGATGGAGGTGCAGGCCAATGTGGAGAAGTCGCTGACACTGTTCGGGCAG
CTGCTGACCAAGAAGCACTTCCTGCTGACCTTCATCCGCACGCTGGAGGCACAGCGCAGC
TTCTCCATGCGCGACCGCGGGAATGTGGCCTCGCTCATCATGACGGCCCTGCAGGGCGAG
ATGGAATACGCCACAGGCGTGCTCAAGCAGCTGCTTTCCGACCTCATCGAGAAGAACCTG
GAGAGCAAGAACCACCCCAAGCTGCTACTGCGCCGGACTGAGTCGGTGGCAGAGAAGATG
CTAACTAACTGGTTCACCTTCCTCTTGTATAAGTTCCTCAAGGAGTGCGCTGGGGAGCCG
CTGTTCATGCTGTACTGCGCCATCAAGCAGCAGATGGAGAAGGGCCCCATTGACGCCATC
ACGGGTGAGGCACGCTACTCCCTGAGTGAGGACAAGCTCATCCGGCAGCAGATTGACTAC
AAGACACTGACCCTGAACTGTGTGAACCCTGAGAATGAGAATGCACCTGAGGTGCCGGTG
AAGGGGCTGGACTGTGACACGGTCACCCAGGCCAAGGAGAAGCTGCTGGACGCTGCCTAC
AAGGGCGTGCCCTACTCCCAGCGGCCCAAGGCCGCGGACATGGACCTGGAGTGGCGCCAG
GGCCGCATGGCGCGCATCATCCTGCAGGACGAGGACGTCACCACCAAGATTGACAACGAT
TGGAAGAGGCTGAACACACTGGCTCACTACCAGGTGACAGACGGGTCCTCGGTGGCACTG
GTGCCCAAGCAGACGTCCGCCTACAACATCTCCAACTCCTCCACCTTCACCAAGTCCCTC
AGCAGATACGAGAGCATGCTGCGCACGGCCAGCAGCCCCGACAGCCTGCGCTCGCGCACG
CCCATGATCACGCCCGACCTGGAGAGCGGCACCAAGCTGTGGCACCTGGTGAAGAACCAC
GACCACCTGGACCAGCGTGAGGGTGACCGCGGCAGCAAGATGGTCTCGGAGATCTACTTG
ACACGGCTACTGGCCACCAAGGGCACACTGCAGAAGTTTGTGGACGACCTGTTTGAGACC
ATCTTCAGCACGGCACACCGGGGCTCAGCCCTGCCGCTGGCCATCAAGTACATGTTCGAC
TTCCTGGATGAGCAGGCCGACAAGCACCAGATCCACGATGCTGACGTGCGCCACACCTGG
AAGAGCAACTGCCTGCCCCTGCGCTTCTGGGTGAACGTGATCAAGAACCCACAGTTTGTG
TTCGACATTCACAAGAACAGCATCACGGACGCCTGCTTGTCGGTGGTGGCCCAGACCTTC
ATGGACTCCTGCTCCACCTCTGAGCACAAGCTGGGCAAGGACTCACCCTCCAACAAGCTG
CTCTACGCCAAGGACATCCCCAACTACAAGAGCTGGGTGGAGAGGTACTATGCAGACATC
GCCAAGATGCCAGCCATCAGCGACCAGGACATGAGTGCGTATCTGGCTGAGCAGTCCCGC
CTGCACCTGAGCCAGTTCAACAGCATGAGCGCCTTGCACGAGATCTACTCCTACATCACC
AAGTACAAGGATGAGATCCTGGCAGCCCTGGAGAAGGATGAGCAGGCGCGGCGGCAGCGG
CTGCGGAGCAAGCTGGAGCAGGTGGTGGACACGATGGCCCTGAGCAGCTTG
Protein sequence predicted based on nucleotide sequence
MPLPPRSLQVLLLLLLLLLLLPGMWAEAGLPRAGGGSQPPFRTFSASDWGLTHLVVHEQT GEVYVGAVNRIYKLSGNLTLLRAHVTGPVEDNEKCYPPPSVQSCPHGLGSTDNVNKLLLL DYAANRLLACGSASQGICQFLRLDDLFKLGEPHHRKEHYLSSVQEAGSMAGVLIAGPPGQ GQAKLFVGTPIDGKSEYFPTLSSRRLMANEEDADMFGFVYQDEFVSSQLKIPSDTLSKFP AFDIYYVYSFRSEQFVYYLTLQLDTQLTSPDAAGEHFFTSKIVRLCVDDPKFYSYVEFPI GCEQAGVEYRLVQDAYLSRPGRALAHQLGLAEDEDVLFTVFAQGQKNRVKPPKESALCLF TLRAIKEKIKERIQSCYRGEGKLSLPWLLNKELGCINSPLQIDDDFCGQDFNQPLGGTVT IEGTPLFVDKDDGLTAVAAYDYRGRTVVFAGTRSGRIRKILVDLSNPGGRPALAYESVVA QEGSPILRDLVLSPNHQYLYAMTEKQVTRVPVESCVQYTSCELCLGSRDPHCGWCVLHSI CSRRDACERADEPQRFAADLLQCVQLTVQPRNVSVTMSQVPLVLQAWNVPDLSAGVNCSF EDFTESESVLEDGRIHCRSPSAREVAPITRGQGDQRVVKLYLKSKETGKKFASVDFVFYN CSVHQSCLSCVNGSFPCHWCKYRHVCTHNVADCAFLEGRVNVSEDCPQILPSTQIYVPVG VVKPITLAARNLPQPQSGQRGYECLFHIPGSPARVTALRFNSSSLQCQNSSYSYEGNDVS DLPVNLSVVWNGNFVIDNPQNIQAHLYKCPALRESCGLCLKADPRFECGWCVAERRCSLR HHCAADTPASWMHARHGSSRCTDPKILKLSPETGPRQGGTRLTITGENLGLRFEDVRLGV RVGKVLCSPVESEYISAEQIVCEIGDASSVRAHDALVEVCVRDCSPHYRALSPKRFTFVT PTFYRVSPSRGPLSGGTWIGIEGSHLNAGSDVAVSVGGRPCSFSWRNSREIRCLTPPGQS PGSAPIIININRAQLTNPEVKYNYTEDPTILRIDPEWSINSGGTLLTVTGTNLATVREPR IRAKYGGIERENGCLVYNDTTMVCRAPSVANPVRSPPELGERPDELGFVMDNVRSLLVLN STSFLYYPDPVLEPLSPTGLLELKPSSPLILKGRNLLPPAPGNSRLNYTVLIGSTPCTLT VSETQLLCEAPNLTGQHKVTVRAGGFEFSPGTLQVYSDSLLTLPAIVGIGGGGGLLLLVI VAVLIAYKRKSRDADRTLKRLQLQMDNLESRVALECKEAFAELQTDIHELTNDLDGAGIP FLDYRTYAMRVLFPGIEDHPVLKEMEVQANVEKSLTLFGQLLTKKHFLLTFIRTLEAQRS FSMRDRGNVASLIMTALQGEMEYATGVLKQLLSDLIEKNLESKNHPKLLLRRTESVAEKM LTNWFTFLLYKFLKECAGEPLFMLYCAIKQQMEKGPIDAITGEARYSLSEDKLIRQQIDY KTLTLNCVNPENENAPEVPVKGLDCDTVTQAKEKLLDAAYKGVPYSQRPKAADMDLEWRQ GRMARIILQDEDVTTKIDNDWKRLNTLAHYQVTDGSSVALVPKQTSAYNISNSSTFTKSL SRYESMLRTASSPDSLRSRTPMITPDLESGTKLWHLVKNHDHLDQREGDRGSKMVSEIYL TRLLATKGTLQKFVDDLFETIFSTAHRGSALPLAIKYMFDFLDEQADKHQIHDADVRHTW KSNCLPLRFWVNVIKNPQFVFDIHKNSITDACLSVVAQTFMDSCSTSEHKLGKDSPSNKL LYAKDIPNYKSWVERYYADIAKMPAISDQDMSAYLAEQSRLHLSQFNSMSALHEIYSYIT KYKDEILAALEKDEQARRQRLRSKLEQVVDTMALSSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
