Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00861499
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6738nts
Open reading frame: 1 to 6738
ATGCCAGAAATGACAGAGAATGAGACGCCTACAAAAAAGCAGCACAGAAAGAAAAACCGG
GAGACACACAATGCAGTGGAGAGGCATAGAAAGAAGAAAATTAATGCTGGGATAAACAGA
ATAGGAGAGCTGATCCCATGTTCTCCTGCCCTGAAGCAGAGCAAGAATATGATCCTGGAC
CAAGCCTTTAAATATATAACAGAATTGAAAAGGCAAAATGATGAACTCCTGCTTAATGGA
GGAAACAATGAACAAGCTGAAGAAATAAAAAAGCTACGGAAACAACTGGAAGAAATCCAA
AAAGAAAATGGCCGATATATTGAATTACTGAAAGCTAATGACATATGCTTATATGATGAC
CCGACAATTCACTGGAAAGGAAATCTTAAAAACTCAAAGGTCTCTGTTGTTATTCCTAGT
GACCAGGTTCAAAAAAAAATTATTGTTTATTCCAACGGGAATCAGCCTGGTGGAAACAGC
CAGGGAACAGCTGTTCAGGGGATAACTTTTAATGTTAGTCATAATTTACAAAAGCAGACC
GCCAATGTGGTGCCAGTACAGAGGACTTGCAATCTTGTGACTCCTGTATCTATTTCTGGA
GTTTACCCTTCTGAAAACAAGCCATGGCATCAGACCACAGTTCCTGCATTGGCTACCAAC
CAGCCTGTTCCTCTTTGTCTTCCTGCTGCCATTTCTGCTCAGAGTATTCTCGAGCTTCCC
ACCTCTGAAAGCGAATCAAATGTGCTTGGTGCCACTAGTGGCTCACTGATTGCTGTTTCA
ATTGAATCTGAGCCTCACCAACATCATTCTTTGCACACATGCCTAAATGATCAAAATTCT
TCTGAAAATAAGAATGGACAAGAGAACCCCAAAGTATTGAAGAAAATGACCCCTTGTGTT
ACAAACATCCCCCACAGCTCCTCAGCAACTGCCACTAAAGTGCACCATGGAAACAAGTCC
TGCCTGAGCATACAGGACTTCAGAGGTGATTTTCAAAACACTTTTGTTGTTTCAGTTACC
ACCACAGTCTGCTCGCAGCCTCCCAGAACTGCAGGTGATTCTTCTCCAATGAGCATTAGT
AAGAGTGCAGACTTGACAAGTACAGCTACAGTGGTGGCATCATCTGCCCCTGGAGTAGGG
AAGGCCACCATTCCTATAAGCACTCTTTCGGGAAACCCTTTGGACAATGGTTGGACTCTT
TCTTGTTCTTTGCCTTCTTCAAGTGTTAGTACTTCAGATTTGAAAAACATTAATAGCCTT
ACACGAATCTCTTCAGCTGGAAACACACAGACAACGTGGACTACTTTGCAACTGGCAGGA
AACACTATTCAGCCCTTAAGCCAGACACCATCTTCTGCTGTGACTCCAGTATTAAATGAG
TCTGGTACTAGCCCCACCACAAGCAACCACAGTAGATATGTGGCTACAGACATCAACTTG
AATAATTCCTTTCCAGCAGATGGGCAGCCAGTTGAGCAAGTAGTTGTAACATTGCCTTCT
TGTCCATCTTTACCTATGCAGCCACTAATTGCCCAGCCACAAGTTAAATCTCAGCCTCCC
AAAAATATCCTTCCACTGAATTCAGCAATGCAAGTGATTCAGATGGCTCAGCCAGTTGGG
TCAGCTGTTAATTCAGCTCCAACTAATCAAAATGTTATAATTCTTCAGCCACCTAGCACC
ACCCCATGCCCAACAGTGATGAGGGCAGAAGTTTCCAACCAAACAGTAGGTCAACAGATA
GTAATCATACAGGCTGCTAATCAGAATCCTTTGCCACTCCTCCCTGCTCCACCTCCTGGT
TCTGTTCGACTCCCCATCAATGGAGCCAATACTGTAATAGGGTCTAATAATTCAGTGCAA
AATGTTCCAACACCACAGACTTTTGGAGGAAAGCATCTTGTCCACATATTACCAAGACCT
TCATCTTTATCAGCGTCTAACTCAACACAAACTTTTTCTGTTACCATGTCAAACCAACAG
CCTCAAACCATTTCTTTAAATGGACAGCTCTTTGCTTTGCAGCCTGTGATGTCTTCATCA
GGAACTACAAATCAAACCCCTATGCAAATTATTCAACCCACCACCAGCGAAGATCCAAAT
ACCAATGTTGCCCTGAATACATTTGGTGCTTTGGCCAGCCTCAATCAAAGCATATCACAG
ATGGCTGGGCAAAGCTGTGTACAATTGTCTATTAGCCAGCCTGCCAATTCTCAAACTGCT
GCAAATAGTCAAACCACTACAGCTAACTGTGTTTCATTAACAACAACTGCAGCACCTCCT
GTGACAACAGATAGTTCAGCCACACTAGCTAGTACTTATAATCTAGTGAGTACTTCCTCA
ATGAACACTGTTGCTTGTTTGCCTAACATGAAATCTAAAAGGTTGAATAAGAAGCCAGGT
GGCAGGAAACACTTAGCAGCAAACAAGTCAGCGTGTCCCCTGAATTCAGTCAGAGATGTG
AGCAAGTTAGACTGCCCCAACACTGAAGGCTCAGCAGAGCCACCCTGTAATGATGGACTG
CTAGAAAGCTTCCCTGCTGTGTTACCATCTGTCTCTGTGTCTCAGGCAAATAGTGTGAGT
GTTTCTGCTTCACATTCTTTGGGTGTTCTAAGCTCTGAATCATTAATACCTGAGTCTGTA
TCGAAATCTAAGTCAGCAGAAAAGTCCAGCCCACCCTCCCAAGAATCTGTAACAAGTGAA
CATTTTGCAATGGCCGCAGCAAAATCCAAAGATTCTACCCCTAATCTACAACAAGAGACA
TCTCAGGATAAACCACCAAGTAGTTTAGCATTATCAGATGCTGCCAAACCCTGCGCTTCA
GCCAATGTATTGATTCCATCTCCAAGTGATCCTCACATTTTGGTTTCTCAGGTTCCTGGT
TTGTCATCTACAACAAGCACTACAAGTACTGACTGTGTTTCTGAGGTAGAAATCATTGCT
GAACCTTGCAGAGTTGAGCAAGATTCATCAGATACAATGCAAACCACAGGTCTCTTAAAG
GGGCAAGGTTTAACTACATTGCTATCTGATCTTGCTAAAAAAAAAAACCCTCAGAAATCA
TCTCTTTCTGATCAGATGGATCATCCTGACTTTTCTTCAGAAAATCCTAAAATAGTTGAT
TCAAGTGTGAATTTACATCCCAAACAGGAACTATTACTGATGAACAATGATGATAGAGAT
CCTCCACAGCATCATTCCTGCCTCCCTGATCAAGAGGTTATTAATGGTTCTTTGATCAAC
GGTAGACAGGCCGACTCTCCCATGTCAACCAGCTCTGGCAGTAGTCGTAGTTTCTCAGTT
GCATCCATGCTTCCTGAAACAACAAGAGAAGATGTGACCAGCAATGCAACAACTAATACA
TGTGACAGCTGTACCTTTGTAGAGCAAACTGATATAGTAGCTCTTGCAGCAAGAGCTATT
TTTGACCAGGAGAACCTTGAGAAGGGGAGAGTTGGCCTCCAGGCTGATATAAGGGAAGTT
GCTTCAAAGCCTTCTGAAGCATCATTGTTAGAGGGAGACCCACCCTTCAAATCACAGATA
CCTAAAGAGAGTGGCACAGGACAGGCAGAAGCAACACCAAATGAATTTAATTCTCAGGGT
TCAATTGAAGCAACTATGGAGAGGCCCCTTGAGAAACCAAGTTGTTCTCTAGGAATTAAA
ACATCAAATGCATCTTTACAGGATTCAACTTCTCAGCCACCAAGCATCACCAGTTTAAGT
GTGAATAATCTTATCCATCAGAGCAGCATCAGCCATCCTCTGGCCAGCTGTGCGGGTTTA
TCCCCAACTTCAGAGCAAACAACTGTGCCTGCAACGGTTAATCTGACCGTTTCATCTAGC
TCCTATGGCAGTCAACCTCCTGGACCATCTCTGATGACTGAATATTCCCAAGAACAGCTA
AATACTATGACTAGTACCATACCAAATTCACAGATTCAAGAGCCACTCTTAAAGCCAAGC
CATGAAAGCCGTAAGGATTCTGCTAAGCGTGCTGTCCAAGATGACCTTTTACTGTCTTCA
GCTAAACGTCAAAAGCACTGTCAGCCAGCCCCCCTCAGGCTTGAAAGTATGTCCCTGATG
AGCAGAACTCCAGACACCATTTCTGACCAAACTCAAATGATGGTCAGTCAGATCCCTCCT
AATTCTTCAAACTCAGTTGTGCCTGTTAGCAACCCAGCTCATGGAGATGGCCTTACACGA
TTATTTCCACCTAGTAACAACTTTGTGACTCCTGCATTGAGGCAAACTGAAGTTCAGTGT
GGTTCTCAGCCTTCAGTTGCTGAACAGCAGCAAACCCAGGCAAGTCAGCATCTACAGGCC
CTGCAGCAGCATGTTCCAGCTCAAGGTGTATCTCACCTTCATAGTAACCATCTCTACATA
AAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAACAACAACAAGCAGGG
CAGTTAAGAGAGAGGCATCACTTATATCAAATGCAGCATCATGTACCTCATGCAGAGAGC
TCTGTCCACTCTCAGCCCCATAATGTCCACCAACAGAGGACTCTGCAACAGGAAGTTCAG
ATGCAGAAAAAGAGGAATCTTGTTCAGGGCACCCAGACCTCTCAGCTTTCCTTACAACCA
AAGCACCATGGAACTGACCAATCCCGATCCAAGACTGGCCAGCCACATCCCCACCATCAG
CAGATGCAGCAACAAATGCAGCAACACTTTGGAAGCTCCCAGACAGAGAAGAGCTGTGAA
AACCCTTCAACTAGTCGGAACCATCATAACCATCCCCAGAACCATCTCAATCAAGATATT
ATGCACCAACAGCAGGATGTTGGAAGCAGACAGCAAGGTTCAGGGGTTTCATCTGAACAT
GTATCTGGCCATAATCCAATGCAGAGGCTTTTGACATCAAGAGGCTTAGAGCAGCAAATG
GTGTCCCAACCAAGTATTGTGACTAGATCTTCAGACATGACCTGTACTCCACACAGGCCA
GAGAGAAATAGAGTTTCAAGTTATTCTGCTGAGGCACTCATTGGAAAGACATCTTCTAAT
TCAGAGCAGAGAATGGGGATATCAATTCAGGGTTCCAGAGTTTCAGATCAGCTTGAAATG
AGAAGCTATCTTGATGTTCCCAGAAATAAGAGTTTGGCTATTCATAATATGCAGGGTCGT
GTGGACCATACTGTGGCCTCAGATATCCGCCTTTCTGATTGTCAGACGTTTAAACCAAGT
GGAGCTAGTCAACAGCCCCAGAGTAATTTTGAAGTACAATCTTCAAGAAACAATGAAATA
GGTAACCCTGTATCATCATTGCGGAGTATGCAGTCCCAGGCTTTTCGAATTAGTCAAAAC
ACTGGCCCCCCACCAATTGACCGTCAAAAGAGATTATCTTACCCACCAGTTCAGAGCATC
CCAACAGGAAATGGTATTCCATCAAGGGACAGTGAAAATACTTGTCACCAAAGTTTCATG
CAGAGCTTACTTGCCCCTCACCTCAGTGATCAGGTCATTGGGAGCCAGAGGTCACTCTCA
GAACATCAGAGGAATACACAGTGTGGTCCATCCTCTGCAATTGAATATAATTGTCCCCCA
ACTCATGAAAATGTCCATATTAGAAGAGAGAGTGAGAGTCAGAATAGGGAAAGTTGTGAC
ATGTCGTTAGGTGCAATTAACACCAGGAACAGCACCTTGAATATTCCTTTTTCAAGTTCC
TCTTCCTCAGGAGATATTCAAGGTCGAAACACAAGCCCCAATGTTTCTGTACAGAAATCC
AATCCCATGAGGATTACTGAGAGTCATGCCACCAAGGGCCACATGAACCCTCCAGTCACA
ACCAACATGCATGGGGTTGCAAGGCCAGCGTTGCCACATCCATCTGTGTCTCATGGAAAT
GGCGATCAAGGCCCTGCTGTACGTCAAGCTAATTCTTCAGTTCCCCAGAGATCAAGGCAT
CCCCTGCAAGACAGCAGTGGTTCCAAAATTCGTCAGCCTGAAAGGAATCGTTCTGGAAAC
CAAAGGCAAAGTACTGTCTTTGATCCAAGTCTTCCCCATCTTCCTCTCTCTACTGGTGGC
AGTATGATTCTTGGACGTCAACAACCTGCCACAGAGAAGAGAGGAAGTATTGTTCGTTTC
ATGCCTGATAGCCCACAAGTACCTAATGATAATTCAGGTCCTGACCAGCATACACTATCA
CAAAATTTTGGTTTTTCTTTTATTCCTGAGGGTGGCATGAATCCACCAATAAATGCTAAT
GCTTCTTTCATTCCACAGGTTACTCAGCCTAGTGCCACTCGAACTCCAGCCCTCATCCCG
GTAGATCCGCAAAATACTCTGCCCTCCTTCTATCCTCCATACTCTCCTGCTCATCCTACA
CTGTCCAATGATATTTCAATCCCCTATTTTCCTAATCAGATGTTCTCAAATCCTAGCACA
GAGAAGGTAAACAGTGGAAGTTTAAATAACCGATTTGGATCAATTTTATCTCCTCCCAGA
CCTGTTGGGTTTGCTCAACCAAGTTTTCCTCTTCTCCCAGATATGCCACCAATGCACATG
ACCAACTCTCACTTATCCAATTTTAATATGACATCTTTGTTTCCAGAAATAGCCACAGCG
CTTCCTGATGGCTCAGCAATGTCACCTTTGCTTACAATAGCAAATTCCTCTGCCTCTGAC
TCTTCCAAGCAGTCCTCAAACAGACCTGCCCATAACATAAGCCATATTCTAGGTCATGAT
TGCAGTTCAGCTGTTTTG
Protein sequence predicted based on nucleotide sequence
MPEMTENETPTKKQHRKKNRETHNAVERHRKKKINAGINRIGELIPCSPALKQSKNMILD QAFKYITELKRQNDELLLNGGNNEQAEEIKKLRKQLEEIQKENGRYIELLKANDICLYDD PTIHWKGNLKNSKVSVVIPSDQVQKKIIVYSNGNQPGGNSQGTAVQGITFNVSHNLQKQT ANVVPVQRTCNLVTPVSISGVYPSENKPWHQTTVPALATNQPVPLCLPAAISAQSILELP TSESESNVLGATSGSLIAVSIESEPHQHHSLHTCLNDQNSSENKNGQENPKVLKKMTPCV TNIPHSSSATATKVHHGNKSCLSIQDFRGDFQNTFVVSVTTTVCSQPPRTAGDSSPMSIS KSADLTSTATVVASSAPGVGKATIPISTLSGNPLDNGWTLSCSLPSSSVSTSDLKNINSL TRISSAGNTQTTWTTLQLAGNTIQPLSQTPSSAVTPVLNESGTSPTTSNHSRYVATDINL NNSFPADGQPVEQVVVTLPSCPSLPMQPLIAQPQVKSQPPKNILPLNSAMQVIQMAQPVG SAVNSAPTNQNVIILQPPSTTPCPTVMRAEVSNQTVGQQIVIIQAANQNPLPLLPAPPPG SVRLPINGANTVIGSNNSVQNVPTPQTFGGKHLVHILPRPSSLSASNSTQTFSVTMSNQQ PQTISLNGQLFALQPVMSSSGTTNQTPMQIIQPTTSEDPNTNVALNTFGALASLNQSISQ MAGQSCVQLSISQPANSQTAANSQTTTANCVSLTTTAAPPVTTDSSATLASTYNLVSTSS MNTVACLPNMKSKRLNKKPGGRKHLAANKSACPLNSVRDVSKLDCPNTEGSAEPPCNDGL LESFPAVLPSVSVSQANSVSVSASHSLGVLSSESLIPESVSKSKSAEKSSPPSQESVTSE HFAMAAAKSKDSTPNLQQETSQDKPPSSLALSDAAKPCASANVLIPSPSDPHILVSQVPG LSSTTSTTSTDCVSEVEIIAEPCRVEQDSSDTMQTTGLLKGQGLTTLLSDLAKKKNPQKS SLSDQMDHPDFSSENPKIVDSSVNLHPKQELLLMNNDDRDPPQHHSCLPDQEVINGSLIN GRQADSPMSTSSGSSRSFSVASMLPETTREDVTSNATTNTCDSCTFVEQTDIVALAARAI FDQENLEKGRVGLQADIREVASKPSEASLLEGDPPFKSQIPKESGTGQAEATPNEFNSQG SIEATMERPLEKPSCSLGIKTSNASLQDSTSQPPSITSLSVNNLIHQSSISHPLASCAGL SPTSEQTTVPATVNLTVSSSSYGSQPPGPSLMTEYSQEQLNTMTSTIPNSQIQEPLLKPS HESRKDSAKRAVQDDLLLSSAKRQKHCQPAPLRLESMSLMSRTPDTISDQTQMMVSQIPP NSSNSVVPVSNPAHGDGLTRLFPPSNNFVTPALRQTEVQCGSQPSVAEQQQTQASQHLQA LQQHVPAQGVSHLHSNHLYIKQQQQQQQQQQQQQQQQQAGQLRERHHLYQMQHHVPHAES SVHSQPHNVHQQRTLQQEVQMQKKRNLVQGTQTSQLSLQPKHHGTDQSRSKTGQPHPHHQ QMQQQMQQHFGSSQTEKSCENPSTSRNHHNHPQNHLNQDIMHQQQDVGSRQQGSGVSSEH VSGHNPMQRLLTSRGLEQQMVSQPSIVTRSSDMTCTPHRPERNRVSSYSAEALIGKTSSN SEQRMGISIQGSRVSDQLEMRSYLDVPRNKSLAIHNMQGRVDHTVASDIRLSDCQTFKPS GASQQPQSNFEVQSSRNNEIGNPVSSLRSMQSQAFRISQNTGPPPIDRQKRLSYPPVQSI PTGNGIPSRDSENTCHQSFMQSLLAPHLSDQVIGSQRSLSEHQRNTQCGPSSAIEYNCPP THENVHIRRESESQNRESCDMSLGAINTRNSTLNIPFSSSSSSGDIQGRNTSPNVSVQKS NPMRITESHATKGHMNPPVTTNMHGVARPALPHPSVSHGNGDQGPAVRQANSSVPQRSRH PLQDSSGSKIRQPERNRSGNQRQSTVFDPSLPHLPLSTGGSMILGRQQPATEKRGSIVRF MPDSPQVPNDNSGPDQHTLSQNFGFSFIPEGGMNPPINANASFIPQVTQPSATRTPALIP VDPQNTLPSFYPPYSPAHPTLSNDISIPYFPNQMFSNPSTEKVNSGSLNNRFGSILSPPR PVGFAQPSFPLLPDMPPMHMTNSHLSNFNMTSLFPEIATALPDGSAMSPLLTIANSSASD SSKQSSNRPAHNISHILGHDCSSAVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
