Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00862174
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5550nts
Open reading frame: 1 to 5550
ATGGCGGTCTCCAGGCTAGATCGCCTTTTTATTTTACTGGATACTGGCACTACTCCTGTT
ACAAGAAAAGCTGCTGCACAGCAACTTGGAGAAGTGGTGAAGCTTCATCCCCATGAACTA
AATAATCTCCTGTCTAAAGTGTTGATATATTTAAGGAGTGCAAATTGGGATACCCGGATT
GCAGCAGGACAAGCTGTTGAAGCTATAGTGAAAAATGTACCTGAGTGGAATCCAGTGCCG
AGAACCAGACAAGAACCTACTTCCGAAAGTTCTATGGAAGATTCACCTACTACAGAGCGA
TTGAATTTTGACAGATTTGATATATGTAGATTGTTACAACATGGTGCATCACTCCTGGGA
TCTGCTGGTGCCGAATTTGAAGTCCAAGATGAAAAATCAGGTGAAGTGGATCCTAAAGAG
AGGATAGCACGCCAACGAAAATTATTACAGAAGAAACTTGGCCTTAATATGGGAGAAGCA
ATTGGAATGAGTACTGAAGAACTTTTCAATGATGAGGATTTGGATTATACCCCAACTTCA
GCATCCTTTGTTAACAAACAACCTACTCTTCAGGCAGCTGAATTGATTGACTCAGAGTTT
CGAGCAGGAATGAGCAATAGACAAAAGAACAAAGCTAAAAGAATGGCCAAGTTATTTGCA
AAACAGAGATCCAGGGATGCAGTGGAAACTAATGAGAAGAGCAATGATAGCACTGATGGG
GAGCCAGAAGAAAAGAGACGGAAAATAGCAAATGTTGTTATTAATCAGTCTGCAAATGAT
TCCAAAGTCTTGATTGATAATATTCCAGACAGCTCTTCCTTAATTGAAGAGACAAATGAA
TGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAG
GTTCGACATGGTGCAGGCACTGGACTTAGGGAAATTCTTAAAGCTCATGGGAAAAGTGGT
GGTAAAATGGGTGACAGCACTTTAGAAGAGATGATTCAGCAGCATCAAGAGTGGTTGGAA
GACTTGGTTATTAGACTCCTTTGTGTTTTTGCATTAGACAGATTTGGAGACTTTGTTTCT
GATGAAGTTGTGGCACCAGTTCGTGAAACTTGTGCTCAAACATTAGGTGTGGTTTTAAAA
CACATGAACGAAACAGGAGTTCATAAGACTGTGGATGTGCTGCTAAAATTACTTACACAA
GAACAATGGGAAGTTAGACATGGTGGTCTGCTGGGAATAAAATATGCTTTGGCAGTCCGT
CAGGATGTAATTAATACTTTATTGCCTAAAGTTTTAACTAGAATAATTGAAGGACTCCAG
GATCTTGATGATGATGTCAGAGCTGTTGCTGCAGCATCATTAGTGCCTGTAGTAGAAAGC
CTTGTCTATCTTCAGACACAAAAGGTGCCCTTCATTATAAATACATTGTGGGATGCTCTT
CTGGAATTAGATGATCTAACAGCTTCAACAAATAGTATTATGACTCTCCTTTCATCCTTG
TTAACTTACCCTCAGGTCCAACAATGCAGTATTCAGCAGTCACTGACAGTTTTAGTTCCA
CGTGTCTGGCCTTTTTTGCATCACACTATATCATCAGTTCGAAGAGCAGCATTGGAAACT
CTGTTTACGTTATTATCAACACAGGACCAGAACTCTTCATCTTGGCTTATACCTATACTG
CCTGATATGCTCCGACACATTTTTCAGTTCTGTGTTTTGGAAAGCAGCCAGGAAATTCTG
GACCTTATTCACAAGGTTTGGATGGAACTGTTGAGTAAGGCTTCAGTTCAGTATGTGGTA
GCAGCTGCTTGCCCATGGATGGGTGCTTGGCTTTGCTTGATGATGCAGCCTTCTCATTTA
CCTATCGATTTAAATATGTTGCTAGAAGTAAAAGCTAGAGCCAAGGAAAAAACAGGTGGT
AAGGTGCGCCAAGGCCAAAGCCAGAATAAAGAAGTACTTCAGGAGTATATTGCAGGTGCC
GACACCATCATGGAAGACCCAGCCACCAGGGATTTTGTAGTTATGCGGGCCAGAATGATG
GCAGCCAAGTTGTTAGGAGCACTTTGTTGCTGTATTTGTGATCCAGGTGTAAATGTGGTA
ACTCAAGAAATTAAACCAGCTGAATCCCTGGGCCAGTTACTACTCTTCCATTTGAACTCC
AAGTCTGCTTTACAGAGGATTAGTGTAGCTTTGGTAATCTGTGAATGGGCTGCTTTACAA
AAGGAGTGTAAAGCTGTTACCTTAGCTGTGCAGCCGCGTTTACTTGATATCCTTTCAGAA
CATTTATATTATGACGAAATTGCCGTTCCATTCACACGAATGCAGAATGAATGTAAACAA
CTTATATCATCATTAGCTGATGTACATATTGAAGTTGGTAATCGAGTAAACAACAATGTT
TTAACAATAGATCAAGCTAGTGACTTGGTCACTACTGTTTTTAATGAAGCCACATCATCT
TTCGATTTAAATCCTCAAGTGTTACAACAGTTAGATAGTAAACGACAGCAGGTCCAAATG
ACAGTTACAGAGACCAACCAGGAGTGGCAAGTGTTGCAGTTGAGAGTGCATACTTTTGCT
GCCTGTGCAGTTGTGAGCTTGCAGCAGCTTCCGGAGAAATTAAATCCTATCATAAAACCA
TTAATGGAGACAATTAAAAAAGAAGAGAATACACTAGTGCAAAACTATGCAGCTCAGTGC
ATAGCTAAACTCCTTCAGCAGTGCACAACAAGGACGCCCTGTCCCAATTCAAAAATTATT
AAAAACCTCTGTAGCTCACTTTGTGTGGACCCATATCTAACTCCTTGTGTCACATGTCCA
GTACCAACACAAAGTGGCCAGGAAAATTCTAAAGGATCCACCTCAGAAAAAGATGGAATG
CACCATACTGTCACCAAGCACAGAGGTATAATTACACTCTACAGGCACCAGAAAGCTGCC
TTTGCTATCACAAGTAGGCGAGGTCCTACCCCCAAAGCAGTAAAAGCTCAAATAGCAGAT
CTTCCTGCAGGAAGTAGTGGAAATATTCTTGTTGAACTTGATGAGGCCCAGAAGCCTTAC
CTGGTACAACGGAGAGGAGCTGAATTTGCTTTGACAACTATAGTAAAGCATTTTGGTGGT
GAAATGGCAGTGAAGTTGCCACATCTCTGGGATGCTATGGTTGGCCCATTGAGGAATACA
ATCGACATAAATAATTTTGATGGAAAATCCCTCCTGGATAAGGGAGATAGCCCTGCTCAA
GAATTGGTGAATTCTCTGCAGGTTTTTGAAACAGCAGCAGCTTCAATGGATTCTGAGCTT
CATCCCTTGTTGGTCCAGCATTTGCCACATCTTTATATGTGCCTTCAATACCCCAGTACT
GCAGTGAGGCACATGGCTGCTCGTTGTGTAGGTGTCATGAGCAAAATAGCTACCATGGAA
ACAATGAATATTTTTTTGGAGAAGGTTCTTCCGTGGCTGGGAGCAATTGATGACAGTGTC
AAACAAGAGGGTGCAATTGAAGCACTTGCCTGTGTAATGGAACAACTAGATGTTGGTATT
GTTCCATATATTGTCCTTTTAGTTGTTCCTGTATTAGGAAGAATGAGTGATCAGACAGAC
AGTGTGAGATTCATGGCCACGCAGTGCTTTGCAACGCTAATTAGACTCATGCCACTTGAG
GCAGGCATTCCAGACCCTCCAAACATGTCAGCAGAATTAATCCAATTGAAAGCCAAGGAG
CGACACTTTTTGGAGCAATTGTTAGATGGGAAAAAATTGGAAAATTATAAAATTCCAGTA
CCAATCAATGCTGAACTCAGAAAATATCAGCAGGATGGTGTGAACTGGTTAGCATTTCTT
AATAAGTATAAACTTCATGGAATTCTGTGTGATGACATGGGTTTAGGAAAAACTTTACAG
TCCATCTGCATTCTAGCAGGAGATCATTGTCATAGGGCCCAGGAATATGCAAGATCAAAA
TTAGCAGAATGTATGCCACTTCCTTCCTTAGTGGTTTGTCCGCCAACATTAACAGGCCAT
TGGGTGGATGAAGTAGGTAAATTTTGCTCTAGAGAATATCTCAACCCGTTGCATTACACT
GGACCTCCCACTGAAAGAATAAGGTTACAGCACCAAGTAAAAAGGCACAATCTAATAGTG
GCTTCATATGATGTTGTGAGGAATGACATAGATTTCTTTAGAAATATTAAATTTAACTAC
TGCATTCTTGATGAAGGCCATGTCATCAAAAATGGAAAAACAAAGTTGTCAAAAGCAGTA
AAACAACTGACTGCTAATTATAGGATTATTCTTTCTGGAACACCAATCCAGAACAACGTT
TTGGAGCTGTGGTCATTATTTGATTTCCTCATGCCAGGATTTTTGGGTACTGAACGCCAG
TTTGCTGCTCGATATGGTAAACCTATATTAGCAAGTAGGGATGCTCGAAGCTCCAGTCGA
GAGCAAGAAGCAGGTGTTCTTGCTATGGATGCGCTGCACCGCCAAGTACTACCGTTTCTT
TTGAGAAGAATGAAAGAAGATGTTTTGCAGGATCTTCCACCTAAAATTATTCAAGACTAT
TATTGTACTCTTAGTCCTCTCCAGGTTCAGCTCTATGAAGATTTTGCTAAGTCTCGTGCC
AAGTGTGATGTTGATGAAACAGTTTCTTCAGCTACACTTTCTGAAGAAACTGAAAAACCA
AAGCTTAAAGCTACAGGCCACGTATTCCAGGCATTACAGTACTTACGTAAACTGTGCAAC
CATCCAGCATTAGTCTTAACACCTCAACATCCAGAATTCAAGACCACTGCCGAAAAACTG
GCAGTTCAGAATTCTTCTCTACATGATATTCAACATGCCCCTAAGCTCTCAGCTTTGAAA
CAATTGCTGTTGGACTGCGGTTTGGGAAATGGCAGCACTTCCGAGAGTGGCACAGAGTCT
GTTGTGGCCCAGCACAGGATACTGATATTCTGTCAGCTGAAAAGCATGCTTGATATAGTA
GAGCATGATCTCCTCAAACCTCACTTGCCCTCTGTCACTTATTTGAGATTAGATGGCAGC
ATACCTCCTGGTCAGAGGCATTCCATTGTTTCCCGGTTTAATAATGATCCATCTATAGAC
GTTCTGTTACTTACCACTCACGTTGGTGGCCTGGGACTTAATTTGACAGGCGCTGACACA
GTAGTATTTGTGGAGCATGACTGGAATCCTATGCGAGATCTACAAGCCATGGACCGGGCC
CATCGCATTGGGCAGAAACGTGTGGTTAACGTATACCGATTGATAACCAGAGGAACATTG
GAAGAAAAAATAATGGGGTTGCAGAAATTCAAGATGAACATAGCGAATACTGTTATTAGC
CAAGAGAATTCTAGTTTGCAGAGCATGGGGACTGATCAGCTTCTTGATCTGTTTACTCTT
GATAAGGATGGCAAAGCAGAAAAAGCTGACACCTCTACTTCTGGGAAAGCAAGTATGAAA
TCAATTCTTGAAAACCTGAGTGATCTTTGGGATCAAGAGCAGTATGATTCAGAGTACAGC
CTGGAAAATTTTATGCATTCTCTCAAGTTG
Protein sequence predicted based on nucleotide sequence
MAVSRLDRLFILLDTGTTPVTRKAAAQQLGEVVKLHPHELNNLLSKVLIYLRSANWDTRI AAGQAVEAIVKNVPEWNPVPRTRQEPTSESSMEDSPTTERLNFDRFDICRLLQHGASLLG SAGAEFEVQDEKSGEVDPKERIARQRKLLQKKLGLNMGEAIGMSTEELFNDEDLDYTPTS ASFVNKQPTLQAAELIDSEFRAGMSNRQKNKAKRMAKLFAKQRSRDAVETNEKSNDSTDG EPEEKRRKIANVVINQSANDSKVLIDNIPDSSSLIEETNEWPLESFCEELCNDLFNPSWE VRHGAGTGLREILKAHGKSGGKMGDSTLEEMIQQHQEWLEDLVIRLLCVFALDRFGDFVS DEVVAPVRETCAQTLGVVLKHMNETGVHKTVDVLLKLLTQEQWEVRHGGLLGIKYALAVR QDVINTLLPKVLTRIIEGLQDLDDDVRAVAAASLVPVVESLVYLQTQKVPFIINTLWDAL LELDDLTASTNSIMTLLSSLLTYPQVQQCSIQQSLTVLVPRVWPFLHHTISSVRRAALET LFTLLSTQDQNSSSWLIPILPDMLRHIFQFCVLESSQEILDLIHKVWMELLSKASVQYVV AAACPWMGAWLCLMMQPSHLPIDLNMLLEVKARAKEKTGGKVRQGQSQNKEVLQEYIAGA DTIMEDPATRDFVVMRARMMAAKLLGALCCCICDPGVNVVTQEIKPAESLGQLLLFHLNS KSALQRISVALVICEWAALQKECKAVTLAVQPRLLDILSEHLYYDEIAVPFTRMQNECKQ LISSLADVHIEVGNRVNNNVLTIDQASDLVTTVFNEATSSFDLNPQVLQQLDSKRQQVQM TVTETNQEWQVLQLRVHTFAACAVVSLQQLPEKLNPIIKPLMETIKKEENTLVQNYAAQC IAKLLQQCTTRTPCPNSKIIKNLCSSLCVDPYLTPCVTCPVPTQSGQENSKGSTSEKDGM HHTVTKHRGIITLYRHQKAAFAITSRRGPTPKAVKAQIADLPAGSSGNILVELDEAQKPY LVQRRGAEFALTTIVKHFGGEMAVKLPHLWDAMVGPLRNTIDINNFDGKSLLDKGDSPAQ ELVNSLQVFETAAASMDSELHPLLVQHLPHLYMCLQYPSTAVRHMAARCVGVMSKIATME TMNIFLEKVLPWLGAIDDSVKQEGAIEALACVMEQLDVGIVPYIVLLVVPVLGRMSDQTD SVRFMATQCFATLIRLMPLEAGIPDPPNMSAELIQLKAKERHFLEQLLDGKKLENYKIPV PINAELRKYQQDGVNWLAFLNKYKLHGILCDDMGLGKTLQSICILAGDHCHRAQEYARSK LAECMPLPSLVVCPPTLTGHWVDEVGKFCSREYLNPLHYTGPPTERIRLQHQVKRHNLIV ASYDVVRNDIDFFRNIKFNYCILDEGHVIKNGKTKLSKAVKQLTANYRIILSGTPIQNNV LELWSLFDFLMPGFLGTERQFAARYGKPILASRDARSSSREQEAGVLAMDALHRQVLPFL LRRMKEDVLQDLPPKIIQDYYCTLSPLQVQLYEDFAKSRAKCDVDETVSSATLSEETEKP KLKATGHVFQALQYLRKLCNHPALVLTPQHPEFKTTAEKLAVQNSSLHDIQHAPKLSALK QLLLDCGLGNGSTSESGTESVVAQHRILIFCQLKSMLDIVEHDLLKPHLPSVTYLRLDGS IPPGQRHSIVSRFNNDPSIDVLLLTTHVGGLGLNLTGADTVVFVEHDWNPMRDLQAMDRA HRIGQKRVVNVYRLITRGTLEEKIMGLQKFKMNIANTVISQENSSLQSMGTDQLLDLFTL DKDGKAEKADTSTSGKASMKSILENLSDLWDQEQYDSEYSLENFMHSLKL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
