Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00862404
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3561nts
Open reading frame: 1 to 3561
ATGGAGGTGGACACCGAGGAGAAGCGGCATCGCACGCGGTCCAAAGGGGTTCGAGTTCCC
GTGGAACCAGCCATACAAGAGCTGTTCAGCTGTCCCACCCCTGGCTGTGACGGCAGTGGT
CATGTCAGTGGCAAATATGCAAGACACAGAAGTGTATATGGTTGTCCCTTGGCGAAAAAA
AGAAAAACACAAGATAAACAGCCCCAGGAACCTGCTCCTAAACGAAAGCCATTTGCCGTG
AAAGCAGACAGCTCCTCAGTGGATGAGTGTGACGACAGTGATGGGACTGAGGACATGGAT
GAGAAGGAGGAGGATGAGGGGGAGGAGTACTCCGAGGACAATGATGAGCCAGGGGATGAG
GACGAGGAGGACGAGGAGGGGGACCGGGAGGAGGAGGAGGAGATCGAGGAGGAGGATGAG
GACGATGACGAGGATGGAGAAGATGTGGAGGATGAAGAAGAGGAAGAGGAGGAGGAGGAG
GAGGAGGAAGAGGAAGAAGAAAACGAAGACCATCAAATGAATTGTCACAATACTCGAATA
ATGCAAGACACAGAAAAGGATGATAACAATAATGACGAATATGACAATTACGATGAACTG
GTGGCCAAGTCATTGTTAAACCTCGGCAAAATCGCTGAGGATGCAGCCTACCGGGCCAGG
ACTGAGTCAGAAATGAACAGCAATACCTCCAATAGTCTGGAAGACGATAGTGACAAAAAC
GAAAACCTGGGTCGGAAAAGTGAGTTGAGTTTAGACTTAGACAGTGATGTTGTTAGAGAA
ACAGTGGACTCCCTTAAACTATTAGCCCAAGGACACGGTGTTGTGCTCTCAGAAAACATG
AATGACAGAAATTATGCAGACAGCATGTCGCAGCAAGACAGTAGAAATATGAATTACGTC
ATGTTGGGGAAGCCCATGAACAACGGACTCATGGAAAAGATGGTGGAGGAGAGCGATGAG
GAGGTGTGTCTGAGCAGTCTGGAGTGTTTGAGGAATCAGTGCTTCGACCTGGCCAGGAAG
CTCAGTGAGACCAACCCGCAGGAGAGGAATCCGCAGCAGAACATGAACATCCGTCAGCAT
GTCCGGCCAGAAGAGGACTTCCCCGGAAGGACGCCGGACAGAAACTACTCGGACATGCTG
AACCTCATGCGGCTGGAGGAGCAGTTGAGCCCCCGGTCGAGAGTGTTTGCCAGCTGTGCG
AAGGAGGATGGGTGTCATGAGCGGGACGACGATACCACCTCTGTGAACTCGGACAGGTCT
GAAGAGGTGTTCGACATGACCAAGGGGAACCTGACCCTGCTGGAGAAAGCCATCGCTTTG
GAAACGGAAAGAGCAAAGGCCATGAGGGAGAAGATGGCCATGGAAGCTGGGAGGAGGGAC
AATATGAGGTCATATGAGGACCAGTCTCCGAGACAACTTCCCGGGGAGGACAGAAAGCCT
AAATCCAGTGACAGCCATGTCAAAAAGCCATACTATGGTAAAGATCCCTCAAGAACAGAA
AAGAAAGAGAGCAAGTGTCCAACCCCCGGGTGTGATGGAACCGGCCACGTAACTGGGCTG
TACCCACATCACCGCAGCCTGTCCGGATGCCCGCACAAAGATAGGGTCCCTCCAGAAATC
CTTGCCATGCATGAAAGTGTCCTCAAGTGCCCCACTCCGGGCTGCACGGGGCGCGGGCAT
GTCAACAGCAACAGGAACTCCCACCGAAGCCTCTCCGGATGCCCGATCGCTGCAGCAGAG
AAACTGGCCAAGGCACAGGAAAAGCACCAGAGCTGCGACGTGTCCAAGTCCAGCCAGGCC
TCGGACCGCGTGCTCAGGCCAATGTGCTTTGTGAAGCAGCTGGAGATTCCTCAGTATGGC
TACAGAAACAATGTCCCCACAACTACGCCGCGTTCCAACCTGGCCAAGGAGCTCGAGAAA
TATTCCAAGACCTCGTTTGAATACAACAGTTACGACAACCATACTTATGGCAAGCGAGCC
ATAGCTCCCAAGGTGCAAACCAGGGATATATCCCCCAAAGGATATGATGATGCGAAGCGG
TACTGCAAGGACCCCAGCCCCAGCAGCAGCAGCACCAGCAGCTACGCGCCCAGCAGCAGC
AGCAACCTGAGCTGCGGCGGGGGCAGCAGCGCCAGCAGCACGTGCAGCAAGAGCAGCTTC
GACTACACGCACGACATGGAGGCGGCCCACATGGCGGCCACCGCCATCCTCAACCTGTCC
ACGCGCTGCCGCGAGATGCCGCAGAACCTGAGCACCAAGCCGCAGGACCTGTGCGCCACG
CGGAACCCTGACATGGAGGTGGATGAGAACGGGACCCTGGACCTCAGCATGAACAAGCAG
AGGCCGCGGGACAGCTGCTGCCCCATCCTGACCCCTCTGGAGCCCATGTCCCCCCAGCAG
CAGGCAGTGATGAACAACCGGTGTTTCCAGCTGGGCGAGGGCGACTGCTGGGACTTGCCC
GTAGACTACACCAAAATGAAACCCCGGAGGATAGACGAGGACGAGTCCAAAGACATTACT
CCAGAAGACTTGGACCCATTCCAGGAGGCTCTAGAAGAAAGACGGTATCCCGGGGAGGTG
ACCATCCCAAGTCCCAAACCCAAGTACCCTCAGTGCAAGGAGAGCAAAAAGGACTTAATA
ACTCTGTCTGGCTGCCCCCTGGCGGACAAAAGCATTCGAAGTATGCTGGCCACCAGCTCC
CAAGAACTCAAGTGCCCCACGCCTGGCTGTGATGGTTCTGGACATATCACCGGCAATTAT
GCTTCTCATCGGAGCCTTTCAGGTTGCCCAAGAGCAAAGAAAAGTGGTATCAGGATAGCA
CAGAGCAAAGAAGATAAAGAAGATCAAGAACCCATCAGGTGTCCGGTCCCCGGGTGCGAC
GGCCAGGGCCACATCACTGGGAAGTACGCGTCCCATCGCAGCGCCTCCGGGTGCCCCTTG
GCGGCCAAGAGGCAGAAAGACGGGTACCTGAATGGCTCCCAGTTCTCCTGGAAGTCGGTC
AAGACGGAAGGCATGTCCTGCCCCACGCCAGGATGCGACGGCTCAGGCCACGTCAGCGGC
AGCTTCCTCACACACCGCAGCTTGTCAGGATGCCCGAGAGCCACGTCAGCGATGAAGAAG
GCAAAGCTTTCTGGAGAGCAGATGCTGACCATCAAACAGCGGGCCAGCAACGGTATAGAA
AATGATGAAGAAATCAAACAGTTAGATGAAGAAATCAAGGAGCTAAATGAATCCAATTCC
CAGATGGAAGCCGATATGATTAAACTCAGAACTCAGATTACCACGATGGAGAGCAACCTG
AAGACCATCGAAGAGGAGAACAAAGTGATTGAGCAGCAGAACGAGTCTCTCCTCCACGAG
CTGGCGAACCTGAGCCAGTCTCTGATCCACAGCCTGGCTAACATCCAGCTGCCGCACATG
GATCCAATCAATGAACAAAATTTTGATGCTTACGTGACTACTTTGACGGAAATGTATACA
AATCAAGATCGTTATCAGAGTCCAGAAAATAAAGCCCTACTGGAAAATATAAAGCAGGCT
GTGAGAGGAATTCAGGTCTTG
Protein sequence predicted based on nucleotide sequence
MEVDTEEKRHRTRSKGVRVPVEPAIQELFSCPTPGCDGSGHVSGKYARHRSVYGCPLAKK RKTQDKQPQEPAPKRKPFAVKADSSSVDECDDSDGTEDMDEKEEDEGEEYSEDNDEPGDE DEEDEEGDREEEEEIEEEDEDDDEDGEDVEDEEEEEEEEEEEEEEEENEDHQMNCHNTRI MQDTEKDDNNNDEYDNYDELVAKSLLNLGKIAEDAAYRARTESEMNSNTSNSLEDDSDKN ENLGRKSELSLDLDSDVVRETVDSLKLLAQGHGVVLSENMNDRNYADSMSQQDSRNMNYV MLGKPMNNGLMEKMVEESDEEVCLSSLECLRNQCFDLARKLSETNPQERNPQQNMNIRQH VRPEEDFPGRTPDRNYSDMLNLMRLEEQLSPRSRVFASCAKEDGCHERDDDTTSVNSDRS EEVFDMTKGNLTLLEKAIALETERAKAMREKMAMEAGRRDNMRSYEDQSPRQLPGEDRKP KSSDSHVKKPYYGKDPSRTEKKESKCPTPGCDGTGHVTGLYPHHRSLSGCPHKDRVPPEI LAMHESVLKCPTPGCTGRGHVNSNRNSHRSLSGCPIAAAEKLAKAQEKHQSCDVSKSSQA SDRVLRPMCFVKQLEIPQYGYRNNVPTTTPRSNLAKELEKYSKTSFEYNSYDNHTYGKRA IAPKVQTRDISPKGYDDAKRYCKDPSPSSSSTSSYAPSSSSNLSCGGGSSASSTCSKSSF DYTHDMEAAHMAATAILNLSTRCREMPQNLSTKPQDLCATRNPDMEVDENGTLDLSMNKQ RPRDSCCPILTPLEPMSPQQQAVMNNRCFQLGEGDCWDLPVDYTKMKPRRIDEDESKDIT PEDLDPFQEALEERRYPGEVTIPSPKPKYPQCKESKKDLITLSGCPLADKSIRSMLATSS QELKCPTPGCDGSGHITGNYASHRSLSGCPRAKKSGIRIAQSKEDKEDQEPIRCPVPGCD GQGHITGKYASHRSASGCPLAAKRQKDGYLNGSQFSWKSVKTEGMSCPTPGCDGSGHVSG SFLTHRSLSGCPRATSAMKKAKLSGEQMLTIKQRASNGIENDEEIKQLDEEIKELNESNS QMEADMIKLRTQITTMESNLKTIEEENKVIEQQNESLLHELANLSQSLIHSLANIQLPHM DPINEQNFDAYVTTLTEMYTNQDRYQSPENKALLENIKQAVRGIQVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
