Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00862412
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4632nts
Open reading frame: 1 to 4632
ATGTATGCAGCAGTGGAACATGGGCCTGTGCTTTGCAGCGATTCCAACATCCTGTGCCTG
TCCTGGAAGGGGCGTGTCCCCAAGAGTGAGAAGGAGAAGCCTGTGTGCAGGAGACGCTAC
TATGAGGAAGGCTGGCTGGCCACGGGCAACGGGCGAGGAGTGGTTGGGGTGACTTTCACC
TCTAGTCACTGTCGCAGGGACAGGAGTACTCCACAGAGGATAAATTTCAACCTCCGGGGC
CACAATAGCGAGGTTGTGCTGGTGAGGTGGAATGAGCCCTACCAGAAACTGGCCACGTGC
GATGCGGACGGAGGCATATTCGTGTGGATTCAGTACGAGGGCAGGTGGTCTGTGGAGCTG
GTCAACGACCGCGGGGCGCAGGTGAGTGATTTCACGTGGAGCCATGATGGAACTCAAGCA
CTTATTTCCTATCGAGATGGGTTTGTCCTGGTTGGGTCTGTCAGTGGACAAAGACACTGG
TCATCCGAAATCAACTTGGAAAGTCAAATTACGTGTGGCATATGGACTCCTGACGACCAA
CAGGTGCTGTTTGGCACGGCCGATGGGCAGGTGATTGTCATGGATTGCCACGGCAGAATG
CTGGCCCACGTCCTCTTGCACGAGTCAGACGGTGTCCTCGGCATGTCCTGGAACTACCCG
ATCTTCCTGGTGGAGGACAGCAGCGAGAGCGACACGGACTCAGATGACTACGCCCCTCCC
CAAGATGGTCCGGCAGCATATCCCATCCCAGTGCAGAACATCAAGCCTCTGCTCACCGTC
AGCTTCACCTCGGGAGACATCAGCTTAATGAACAACTACGATGACTTGTCTCCCACGGTC
ATCCGCTCAGGGCTGAAAGAGGTGGTAGCCCAGTGGTGCACACAGGGGGACTTGCTGGCA
GTCGCTGGGATGGAACGGCAGACCCAGCTTGGTGAGCTTCCCAATGGTCCCCTTCTGAAG
AGTGCCATGGTCAAGTTCTACAATGTTCGTGGGGAGCACATCTTCACACTGGACACTCTC
GTGCAGCGCCCCATCATCTCCATCTGCTGGGGTCACCGGGATTCGAGGCTGTTGATGGCA
TCAGGACCAGCCCTGTACGTGGTGCGTGTGGAGCACCGGGTGTCCAGCCTGCAGCTGCTG
TGCCAGCAGGCCATCGCCAGCACCTTGCGTGAGGACAAGGACGTCAGCAAGCTGACTCTG
CCCCCCCGCCTCTGCTCCTACCTCTCCACTGCCTTCATCCCCACCATCAAGCCCCCAATT
CCAGATCCGAACAACATGAGAGACTTTGTCAGCTACCCATCAGCCGGCAACGAGCGGCTG
CACTGCACCATGAAGCGCACAGAGGACGACCCGGAGGTGGGCGGCCCGTGCTACACGCTC
TACCTGGAGTACCTGGGCGGGCTTGTGCCCATCCTCAAAGGGCGGCGCATCAGCAAGCTG
CGGCCAGAGTTCGTCATCATGGACCCGCGGACAGATAGCAAACCAGATGAAATCTATGGG
AACAGCTTGATTTCTACTGTGATCGACAGCTGCAACTGCTCAGACTCCAGTGACATTGAG
CTGAGTGATGACTGGGCTGCCAAGAAATCTCCCAAAATCTCCAGAGCTAGCAAATCACCC
AAACTCCCAAGGATCAGCATTGAGGCCCGCAAGTCACCCAAGCTGCCCCGGGCTGCTCAG
GAGCTCTCCCGGTCCCCACGGTTGCCCCTGCGCAAGCCCTCTGTGGGCTCGCCCAGCCTG
ACTCGGAGAGAGTTTCCTTTTGAAGACATCACTCAGCACAACTATCTTGCTCAGGTCACG
TCTAATATCTGGGGAACCAAATTTAAGATTGTGGGCTTGGCTGCTTTCCTGCCAACCAAC
CTCGGTGCAGTAATCTATAAAACCAGCCTCCTGCATCTCCAGCCGCGGCAGATGACCATT
TATCTCCCAGAAGTTCGGAAAATTTCCATGGACTATATTAATTTACCTGTCTTCAACCCA
AATGTTTTCAGTGAAGATGAAGATGATTTACCAGTGACAGGAGCATCTGGTGTCCCTGAG
AACAGCCCACCTTGTACCGTGAACATCCCTATTGCACCGATCCACAGCTCGGCTCAGGCT
ATGTCCCCCACGCAGAGCATAGGGCTGGTGCAGTCCCTACTGGCCAATCAGAATGTGCAG
CTAGATGTCCTGACCAACCAGACGACAGCTGTAGGGACAGCAGAACATGCAGGTGACAGT
GCCACCCAGTACCCAGTCTCCAACCGGTACTCCAATCCTGGACAGGTGATTTTCGGAAGC
GTGGAAATGGGCCGCATCATTCAGAACCCCCCTCCACTGTCCCTGCCTCCCCCGCCGCAG
GGGCCCATGCAGCTGTCCACGGTGGGCCATGGAGACCGAGACCACGAACACCTGCAGAAG
TCAGCCAAGGCCCTGCGGCCAACACCGCAGCTGGCAGCTGAGGGGGACGCAGTGGTCTTT
AGTGCCCCCCAGGAGGTCCAGGTGACGAAGATAAACCCTCCACCCCCGTACCCAGGAACC
ATCCCCGCTGCCCCCACCACAGCAGCACCCCCGCCCCCTCTGCCGCCCCCACAGCCCCCA
GTGGATGTGTGCTTGAAGAAGGGCGACTTCTCCCTCTACCCCACGTCAGTGCACTACCAG
ACCCCCCTGGGCTATGAGAGGATCACCACCTTCGACAGCAGTGGCAACGTGGAGGAGGTG
TGCCGGCCCCGCACCCGGATGCTGTGCTCCCAGAACACGTACACCCTCCCCGGCCCGGGT
AGCTCTGCCACCTTGAGGCTCACGGCCACTGAGAAGAAGGTCCCTCAGCCCTGCAGCAGT
GCCACCCTGAACCGCCTGACCGTCCCTCGCTACTCCATCCCCACCGGGGACCCACCCCCG
TATCCTGAAATTGCCAGCCAGCTGGCCCAGGGGCGGGGGGCTGCCCAGAGGTCCGACAAT
AGCCTCATCCACGCTACCCTGCGGAGGAACAACCGTGAGGCTACGCTCAAGATGGCCCAG
CTGGCCGACAGCCCGCGGGCCCCCCTGCAGCCCCTGGCCAAGTCCAAGGGCGGGCCCGGG
GGGGTGGTGACACAGCTCCCAGCGCGGCCCCCACCTGCCCTGTACACCTGCAGTCAGTGC
AGTGGCACAGGGCCCAGCTCACAGCCCGGAGCCTCCCTGGCCCATACCGCCAGCGCCTCC
CCGTTGGCCTCCCAGTCCTCCTACAGCCTCCTGAGCCCACCCGACAGCGCCCGCGACCGC
ACCGACTACGTCAACTCGGCCTTCACGGAGGACGAGGCCCTGTCCCAGCACTGTCAGCTT
GAGAAGCCCTTGAGGCACCCTCCCCTGCCTGAAGCTGCTGTCACCCTGAAACGGCCACCC
CCTTACCAGTGGGACCCCATGCTGGGTGAGGATGTTTGGGTTCCTCAAGAAAGGACAGCA
CAGACTTCAGGGCCCAACCCCTTAAAACTGTCCTCTCTGATGCTGAGTCAGGGCCAGCAC
CTGGACGTGTCCCGACTGCCCTTCATCTCCCCCAAGTCTCCTGCCAGCCCCACTGCCACT
TTCCAAACAGGCTATGGGATGGGAGTGCCATATCCAGGAAGCTATAACAACCCCCCTTTG
CCTGGAGTGCAGGCTCCCTGCTCTCCCAAAGATGCCCTGTCCCCAACGCAGTTTGCACAA
CAGGAGCCTGCTGTGGTCCTTCAGCCGCTGTACCCACCCAGCCTCTCCTATTGCACCCTG
CCCCCCATGTACCCAGGAAGCAGCACGTGCTCTAGTTTACAGCTGCCACCTGTCGCCTTG
CATCCATGGAGTTCCTACAGCGCCTGCCCGCCCATGCAGAACCCCCAGGGCACTCTCCCC
CCAAAGCCACACTTGGTGGTGGAGAAGCCCCTTGTGTCCCCACCACCTGCCGACCTCCAA
AGCCACTTGGGCACAGAGGTGATGGTAGAGACTGCAGACAACTTCCAGGAAGTCCTCTCC
CTGACCGAAAGCCCAGTCCCCCAGCGGACAGAAAAATTTGGAAAGAAGAACCGGAAGCGC
CTGGACAGCCGAGCAGAAGAAGGCAGCGTTCAGGCCATCACTGAGGGCAAAGTGAAGAAG
GAGGCTAGGACTTTGAGTGACTTTAATTCCCTAATCTCCAGCCCACACCTGGGGAGAGAG
AAGAAGAAAGTGAAGAGTCAGAAAGACCAACTGAAGTCAAAGAAGTTGAATAAGACAAAC
GAGTTCCAGGACAGCTCCGAGAGCGAGCCTGAGCTGTTCATCAGCGGGGATGAGCTCATG
AACCAGAGCCAGGGCAGCAGAAAGGGCTGGAAAAGCAAGCGCTCCCCACGGGCCGCCGGC
GAGCTGGAGGAGGCCAAGTGCCGGCGGGCCAGTGAGAAGGAGGACGGGCGGCTGGGCAGC
CAAGGCTTCGTGTACGTGATGGCCAACAAGCAGCCGCTGTGGAACGAGGCCACCCAGGTC
TACCAGCTGGACTTCGGGGGGCGGGTGACCCAGGAGTCCGCCAAGAACTTCCAGATTGAG
TTAGAGGGGCGGCAGGTGATGCAGTTTGGACGGATTGATGGCAGTGCGTACATTCTAGAC
TTCCAGTATCCGTTCTCAGCCGTGCAGGCCTTTGCAGTTGCCCTGGCCAACGTGACTCAG
CGCCTCAAATTG
Protein sequence predicted based on nucleotide sequence
MYAAVEHGPVLCSDSNILCLSWKGRVPKSEKEKPVCRRRYYEEGWLATGNGRGVVGVTFT SSHCRRDRSTPQRINFNLRGHNSEVVLVRWNEPYQKLATCDADGGIFVWIQYEGRWSVEL VNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQITCGIWTPDDQ QVLFGTADGQVIVMDCHGRMLAHVLLHESDGVLGMSWNYPIFLVEDSSESDTDSDDYAPP QDGPAAYPIPVQNIKPLLTVSFTSGDISLMNNYDDLSPTVIRSGLKEVVAQWCTQGDLLA VAGMERQTQLGELPNGPLLKSAMVKFYNVRGEHIFTLDTLVQRPIISICWGHRDSRLLMA SGPALYVVRVEHRVSSLQLLCQQAIASTLREDKDVSKLTLPPRLCSYLSTAFIPTIKPPI PDPNNMRDFVSYPSAGNERLHCTMKRTEDDPEVGGPCYTLYLEYLGGLVPILKGRRISKL RPEFVIMDPRTDSKPDEIYGNSLISTVIDSCNCSDSSDIELSDDWAAKKSPKISRASKSP KLPRISIEARKSPKLPRAAQELSRSPRLPLRKPSVGSPSLTRREFPFEDITQHNYLAQVT SNIWGTKFKIVGLAAFLPTNLGAVIYKTSLLHLQPRQMTIYLPEVRKISMDYINLPVFNP NVFSEDEDDLPVTGASGVPENSPPCTVNIPIAPIHSSAQAMSPTQSIGLVQSLLANQNVQ LDVLTNQTTAVGTAEHAGDSATQYPVSNRYSNPGQVIFGSVEMGRIIQNPPPLSLPPPPQ GPMQLSTVGHGDRDHEHLQKSAKALRPTPQLAAEGDAVVFSAPQEVQVTKINPPPPYPGT IPAAPTTAAPPPPLPPPQPPVDVCLKKGDFSLYPTSVHYQTPLGYERITTFDSSGNVEEV CRPRTRMLCSQNTYTLPGPGSSATLRLTATEKKVPQPCSSATLNRLTVPRYSIPTGDPPP YPEIASQLAQGRGAAQRSDNSLIHATLRRNNREATLKMAQLADSPRAPLQPLAKSKGGPG GVVTQLPARPPPALYTCSQCSGTGPSSQPGASLAHTASASPLASQSSYSLLSPPDSARDR TDYVNSAFTEDEALSQHCQLEKPLRHPPLPEAAVTLKRPPPYQWDPMLGEDVWVPQERTA QTSGPNPLKLSSLMLSQGQHLDVSRLPFISPKSPASPTATFQTGYGMGVPYPGSYNNPPL PGVQAPCSPKDALSPTQFAQQEPAVVLQPLYPPSLSYCTLPPMYPGSSTCSSLQLPPVAL HPWSSYSACPPMQNPQGTLPPKPHLVVEKPLVSPPPADLQSHLGTEVMVETADNFQEVLS LTESPVPQRTEKFGKKNRKRLDSRAEEGSVQAITEGKVKKEARTLSDFNSLISSPHLGRE KKKVKSQKDQLKSKKLNKTNEFQDSSESEPELFISGDELMNQSQGSRKGWKSKRSPRAAG ELEEAKCRRASEKEDGRLGSQGFVYVMANKQPLWNEATQVYQLDFGGRVTQESAKNFQIE LEGRQVMQFGRIDGSAYILDFQYPFSAVQAFAVALANVTQRLKL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
