Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00862738
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5289nts
Open reading frame: 1 to 5289
ATGGAATGCTGCCGTCGGGCAACTCCTGGCACACTGCTCCTCTTTCTGGCTTTCCTGCTC
CTGAGTTCCAGGACCGCACGCTCCGAGGAGGACCGGGACGGCCTATGGGATGCCTGGGGC
CCATGGAGTGAATGCTCACGCACCTGCGGGGGTGGGGCCTCCTACTCTCTGAGGCGCTGC
CTGAGCAGCAAGAGCTGTGAAGGAAGAAATATCCGATACAGAACATGCAGTAATGTGGAC
TGCCCACCAGAAGCAGGTGATTTCCGAGCTCAGCAATGCTCAGCTCATAATGATGTCAAG
CACCATGGCCAGTTTTATGAATGGCTTCCTGTGTCTAATGACCCTGACAACCCATGTTCA
CTCAAGTGCCAAGCCAAAGGAACAACCCTGGTTGTTGAACTAGCACCTAAGGTCTTAGAT
GGTACGCGTTGCTATACAGAATCTTTGGATATGTGCATCAGTGGTTTATGCCAAATTGTT
GGCTGCGATCACCAGCTGGGAAGCACCGTCAAGGAAGATAACTGTGGGGTCTGCAACGGA
GATGGGTCCACCTGCCGGCTGGTCCGAGGGCAGTATAAATCCCAGCTCTCCGCAACCAAA
TCGGATGATACTGTGGTTGCAATTCCCTATGGAAGTAGACATATTCGCCTTGTCTTAAAA
GGTCCTGATCACTTATATCTGGAAACCAAAACCCTCCAGGGGACTAAAGGTGAAAACAGT
CTCAGCTCCACAGGAACTTTCCTTGTGGACAATTCTAGTGTGGACTTCCAGAAATTTCCA
GACAAAGAGATACTGAGAATGGCTGGACCACTCACAGCAGATTTCATTGTCAAGATTCGT
AACTCGGGCTCCGCTGACAGTACAGTCCAGTTCATCTTCTATCAACCCATCATCCACCGA
TGGAGGGAGACGGATTTCTTTCCTTGCTCAGCAACCTGTGGAGGAGGTTATCAGCTGACA
TCGGCTGAGTGCTACGATCTGAGGAGCAACCGTGTGGTTGCTGACCAATACTGTCACTAT
TACCCAGAGAACATCAAACCCAAACCCAAGCTTCAGGAGTGCAACTTGGATCCTTGTCCA
GCCAGTGACGGATACAAGCAGATCATGCCTTATGACCTCTACCATCCCCTTCCTCGGTGG
GAGGCCACCCCATGGACCGCGTGCTCCTCCTCGTGTGGGGGGGGCATCCAGAGCCGGGCA
GTTTCCTGTGTGGAGGAGGACATCCAGGGGCATGTCACTTCAGTGGAAGAGTGGAAATGC
ATGTACACCCCTAAGATGCCCATCGCGCAGCCCTGCAACATTTTTGACTGCCCTAAATGG
CTGGCACAGGAGTGGTCTCCGTGCACAGTGACATGTGGCCAGGGCCTCAGATACCGTGTG
GTCCTCTGCATCGACCATCGAGGAATGCACACAGGAGGCTGTAGCCCAAAAACAAAGCCC
CACATAAAAGAGGAATGCATCGTACCCACTCCCTGCTATAAACCCAAAGAGAAACTTCCA
GTCGAGGCCAAGTTGCCATGGTTCAAACAAGCTCAAGAGCTAGAAGAAGGAGCTGCTGTG
TCAGAGGAGCCCTCGTTCATCCCAGAGGCCTGGTCGGCCTGCACAGTCACCTGTGGTGTG
GGGACCCAGGTGCGAATAGTCAGGTGCCAGGTGCTCCTGTCTTTCTCTCAGTCCGTGGCT
GACCTGCCTATTGACGAGTGTGAAGGGCCCAAGCCAGCATCCCAGCGTGCCTGTTATGCA
GGCCCATGCAGCGGGGAAATTCCTGAGTTCAACCCAGACGAGACAGATGGGCTCTTTGGT
GGCCTGCAGGATTTCGACGAGCTGTATGACTGGGAGTATGAGGGGTTCACCAAGTGCTCC
GAGTCCTGTGGAGGAGGTGTCCAGGAGGCTGTGGTGAGCTGCTTGAACAAACAGACTCGG
GAGCCTGCTGAGGAGAACCTGTGCGTGACCAGCCGCCGGCCCCCACAGCTCCTGAAGTCC
TGCAATTTGGATCCCTGCCCAGCAAGGTGGGAAATTGGCAAGTGGAGTCCATGTAGTCTC
ACATGTGGGGTCGGCCTACAGACCAGAGACGTCTTCTGCAGCCACCTGCTTTCCAGAGAG
ATGAATGAAACAGTCATCCTGGCTGATGAGCTGTGTCGCCAGCCCAAGCCCAGCACGGTG
CAAGCTTGTAACCGCTTTAATTGCCCCCCAGCCTGGTACCCTGCACAGTGGCAGCCGTGT
TCCAGAACGTGTGGCGGGGGTGTTCAGAAACGTGAGGTTCTTTGCAAGCAGCGCATGGCT
GATGGCAGCTTCCTGGAGCTTCCTGAGACCTTCTGTTCAGCTTCAAAACCTGCCTGCCAG
CAAGCATGCAAGAAAGATGACTGTCCCAGCGAGTGGCTTCTCTCAGACTGGACAGAGTGT
TCCACAAGCTGCGGGGAAGGCACCCAGACTCGAAGCGCCATTTGCCGAAAGATGCTGAAA
ACCGGCCTCTCAACGGTTGTCAATTCCACCCTGTGCCCGCCCCTGCCTTTCTCTTCCTCC
ATCAGGCCCTGTATGCTGGCAACCTGTGCAAGGCCCGGGCGGCCATCCACGAAGCACAGC
CCGCACATCGCGGCCGCCAGGAAGGTCTACATACAGACTCGCAGGCAGAGGAAGCTGCAC
TTCGTGGTGGGGGGCTTCGCCTACCTGCTCCCCAAGACGGCGGTGGTGCTGCGCTGCCCG
GCGCGCAGGGTCCGCAAGCCCCTCATCACCTGGGAGAAGGACGGCCAGCACCTCATCAGC
TCGACGCACGTCACGGTGGCCCCCTTCGGCTATCTCAAGATCCACCGCCTCAAGCCCTCG
GATGCAGGCGTCTACACCTGCTCAGCGGGCCCGGCCCGGGAGCACTTTGTGATTAAGCTC
ATCGGAGGCAACCGCAAGCTCGTGGCCCGGCCCTTGAGCCCGAGAAGTGAGGAAGAGGTG
CTTGCGGGGAGGAAGGGCGGCCCGAAGGAGGCCCTGCAGACCCACAAACACCAGAACGGG
ATCTTCTCCAACGGCAGCAAGGCGGAGAAGCGGGGCCTGGCCGCCAACCCGGGGAGCCGC
TACGACGACCTCGTCTCCCGGCTGCTGGAGCAGGGCGGCTGGCCCGGAGAGCTGCTGGCC
TCGTGGGAGGCGCAGGACTCTGCGGAAAGGAACACGACCTCGGAGGAGGACCCGGGTGCA
GAGCAAGTGCTCCTGCACCTGCCCTTCACCATGGTGACCGAGCAGCGGCGCCTGGACGAC
ATCCTGGGGAACCTCTCCCAGCAGCCCGAGGAGCTGCGCGACCTCTACAGCAAGCACCTG
GTGGCCCAGCTGGCCCAGGAGATCTTCCGCAGCCACCTGGAGCACCAGGACACGCTCCTG
AAGCCCTCGGAGCGCAGGACTTCCCCAGTGACTCTCTCGCCTCATAAACACGTGTCTGGC
TTCAGCAGCTCCCTGCGGACCTCCTCCACCGGGGACGCCGGGGGAGGCTCTCGAAGGCCA
CACCGCAAGCCCACCATCCTGCGCAAGATCTCAGCGGCCCAGCAGCTCTCAGCCTCGGAG
GTGGTCACCCACCTGGGGCAGACGGTGGCCCTGGCCAGCGGGACACTGAGTGTTCTTCTG
CACTGTGAGGCCATCGGCCACCCAAGGCCTACCATCAGCTGGGCCAGGAATGGAGAAGAA
GTTCAGTTCAGTGACAGGATTCTTCTACAGCCAGATGATTCCTTACAGATCTTGGCACCA
GTGGAAGCAGATGTGGGTTTCTACACTTGCAATGCCACCAATGCCTTGGGATACGACTCT
GTCTCCATTGCCGTCACATTAGCAGGAAAGCCACTAGTGAAAACGTCACGAATGACAGTG
ATCAACACGGAGAAGCCTGCAGTCACAGTCGATATAGGAAGCACCATCAAAACAGTGCAG
GGAGTGAATGTGACAATCAACTGCCAGGTTGCAGGAGTGCCTGAAGCTGAAGTCACTTGG
TTCAGGAATAAAAGCAAACTGGGCTCCCCGCACCATCTGCACGAAGGCTCCTTGCTGCTC
ACAAACGTGTCCTCCTCGGATCAGGGCCTGTACTCCTGCAGGGCGGCCAATCTTCATGGA
GAGCTGACTGAGAGCACCCAGCTGCTGATCCTAGATCCCCCCCAAGTCCCCACACAGTTG
GAAGACATCAGGGCCTTGCTCGCTGCCACTGGACCGAACCTTCCTTCAGTGCTGACGTCT
CCTCTGGGAACACAGCTGGTCCTGGATCCTGGGAATTCTGCTCTCCTTGGCTGCCCCATC
AAAGGTCACCCTGTCCCTAATATCACCTGGTTTCATGGTGGTCAGCCAATTGTCACTGCC
ACAGGACTGACGCATCACATCTTGGCAGCTGGACAGATCCTTCAAGTTGCAAACCTTAGC
GGTGGGTCTCAAGGGGAATTCAGCTGCCTTGCTCAGAATGAGGCAGGGGTGCTCATGCAG
AAGGCATCTTTAGTGATCCAAGATTACTGGTGGTCTGTGGACAGACTGGCAACCTGCTCA
GCCTCCTGTGGTAACCGGGGGGTTCAGCAGCCCCGCTTGAGGTGCCTGCTGAACAGCACG
GAGGTCAACCCTGCCCACTGCGCAGGGAAGGTTCGCCCTGCGGTGCAGCCCATCGCGTGC
AACCGGAGAGACTGCCCTTCTCGGTGGATGGTGACCTCCTGGTCTGCCTGTACCCGGAGC
TGTGGGGGAGGTGTCCAGACCCGCAGGGTGACCTGTCAAAAGCTGAAAGCCTCTGGGATC
TCCACCCCTGTGTCCAATGACATGTGCACCCAGGTCGCCAAGCGGCCTGTGGACACCCAG
GCCTGTAACCAGCAGCTGTGTGTGGAGTGGGCCTTCTCCAGCTGGGGCCAGTGCAATGGG
CCTTGCATCGGGCCTCACCTAGCTGTGCAACACAGACAAGTCTTCTGCCAGACACGGGAT
GGCATCACCTTACCATCAGAGCAGTGCAGTGCTCTTCCGAGGCCTGTGAGCACCCAGAAC
TGCTGGTCAGAGGCCTGCAGTGTACACTGGAGAGTCAGCCTGTGGACCCTGTGCACAGCT
ACCTGTGGCAACTACGGCTTCCAGTCCCGGCGTGTGGAGTGTGTGCATGCCCGCACCAAC
AAGGCAGTGCCTGAGCACCTGTGCTCCTGGGGGCCCCGGCCTGCCAACTGGCAGCGCTGC
AACATCACCCCATGTGAAAACATGGAGTGCAGAGACACCACCAGGTACTGCGAGAAGGTG
AAACAGCTGAAACTCTGCCAACTCAGCCAGTTTAAATCTCGCTGCTGTGGAACTTGTGGC
AAAGCGTTG
Protein sequence predicted based on nucleotide sequence
MECCRRATPGTLLLFLAFLLLSSRTARSEEDRDGLWDAWGPWSECSRTCGGGASYSLRRC LSSKSCEGRNIRYRTCSNVDCPPEAGDFRAQQCSAHNDVKHHGQFYEWLPVSNDPDNPCS LKCQAKGTTLVVELAPKVLDGTRCYTESLDMCISGLCQIVGCDHQLGSTVKEDNCGVCNG DGSTCRLVRGQYKSQLSATKSDDTVVAIPYGSRHIRLVLKGPDHLYLETKTLQGTKGENS LSSTGTFLVDNSSVDFQKFPDKEILRMAGPLTADFIVKIRNSGSADSTVQFIFYQPIIHR WRETDFFPCSATCGGGYQLTSAECYDLRSNRVVADQYCHYYPENIKPKPKLQECNLDPCP ASDGYKQIMPYDLYHPLPRWEATPWTACSSSCGGGIQSRAVSCVEEDIQGHVTSVEEWKC MYTPKMPIAQPCNIFDCPKWLAQEWSPCTVTCGQGLRYRVVLCIDHRGMHTGGCSPKTKP HIKEECIVPTPCYKPKEKLPVEAKLPWFKQAQELEEGAAVSEEPSFIPEAWSACTVTCGV GTQVRIVRCQVLLSFSQSVADLPIDECEGPKPASQRACYAGPCSGEIPEFNPDETDGLFG GLQDFDELYDWEYEGFTKCSESCGGGVQEAVVSCLNKQTREPAEENLCVTSRRPPQLLKS CNLDPCPARWEIGKWSPCSLTCGVGLQTRDVFCSHLLSREMNETVILADELCRQPKPSTV QACNRFNCPPAWYPAQWQPCSRTCGGGVQKREVLCKQRMADGSFLELPETFCSASKPACQ QACKKDDCPSEWLLSDWTECSTSCGEGTQTRSAICRKMLKTGLSTVVNSTLCPPLPFSSS IRPCMLATCARPGRPSTKHSPHIAAARKVYIQTRRQRKLHFVVGGFAYLLPKTAVVLRCP ARRVRKPLITWEKDGQHLISSTHVTVAPFGYLKIHRLKPSDAGVYTCSAGPAREHFVIKL IGGNRKLVARPLSPRSEEEVLAGRKGGPKEALQTHKHQNGIFSNGSKAEKRGLAANPGSR YDDLVSRLLEQGGWPGELLASWEAQDSAERNTTSEEDPGAEQVLLHLPFTMVTEQRRLDD ILGNLSQQPEELRDLYSKHLVAQLAQEIFRSHLEHQDTLLKPSERRTSPVTLSPHKHVSG FSSSLRTSSTGDAGGGSRRPHRKPTILRKISAAQQLSASEVVTHLGQTVALASGTLSVLL HCEAIGHPRPTISWARNGEEVQFSDRILLQPDDSLQILAPVEADVGFYTCNATNALGYDS VSIAVTLAGKPLVKTSRMTVINTEKPAVTVDIGSTIKTVQGVNVTINCQVAGVPEAEVTW FRNKSKLGSPHHLHEGSLLLTNVSSSDQGLYSCRAANLHGELTESTQLLILDPPQVPTQL EDIRALLAATGPNLPSVLTSPLGTQLVLDPGNSALLGCPIKGHPVPNITWFHGGQPIVTA TGLTHHILAAGQILQVANLSGGSQGEFSCLAQNEAGVLMQKASLVIQDYWWSVDRLATCS ASCGNRGVQQPRLRCLLNSTEVNPAHCAGKVRPAVQPIACNRRDCPSRWMVTSWSACTRS CGGGVQTRRVTCQKLKASGISTPVSNDMCTQVAKRPVDTQACNQQLCVEWAFSSWGQCNG PCIGPHLAVQHRQVFCQTRDGITLPSEQCSALPRPVSTQNCWSEACSVHWRVSLWTLCTA TCGNYGFQSRRVECVHARTNKAVPEHLCSWGPRPANWQRCNITPCENMECRDTTRYCEKV KQLKLCQLSQFKSRCCGTCGKAL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
