Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00863174
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3282nts
Open reading frame: 1 to 3282
ATGAGTTGGTTCAACGCCTCCCAGCTCTCCAGCTTCGCTAAGCAGGCCCTGTCCCAGGCC
CAGAAGTCTATTGACAGGGTTCTGGACATCCAGGAAGAGGAGCCGAGCATCTGGGCCGAG
ACCATTCCGTATGGAGAGCCGGGAATAAGTTCCCCTGTCAGTGGAGGATGGGATACTTCA
ACCTGGGGGTTGAAATCAAACACTGAACCTCAGAGTCCACCAATAGCCTCTCCTAAAGCA
ATCACAAAGCCAGTTCGGAGGACTGTGGTCGATGAATCTGAAAATTTCTTCAGTGCCTTT
CTCTCGCCAACTGATGTCCAGACCATTCAGAAGAGTCCAGTGGTATCAAAACCTCCAGCA
AAATCACAACGACCAGAAGAAGAAGTGAAAAGCAGCTTACATGAATCCTTGCACATTGGC
CAGTCAAGAACTCCTGAAACAACTGAATCACAAGTAAAAGACTCTTCTTTGTGTGTTTCA
GGGGAAACTCTGGCAGCAGGTACTTCATCACCTAAAACTGAAGGCAAGCACGAAGAAACT
GTTAATAAAGAATCGGATATGAAGGTGCCAACTGTAAGTTTGAAAGTATCTGAAAGTGTA
ATTGATGTGAAAACAACTATGGAAAGTATATCTAATACGTCTACGCAGTCTCTCACAGCA
GAAACAAAGGACATAGCTTTGGAACCTAAGGAACAAAAACATGAAGACAGGCAGAGCAAT
ACACCTTCTCCTCCTGTTAGTACCTTTTCATCAGGTACTTCTACCACCAGTGATATTGAA
GTTTTAGATCATGAAAGTGTAATAAGTGAGAGCTCAGCGAGCTCGAGACAAGAGACTACA
GATTCAAAATCAAGTCTTCACTTGATGCAGACATCTTTTCAGCTTCTCTCTGCATCTGCT
TGTCCTGAATATAATCGTTTAGATGATTTCCAAAAACTCACTGAGAGTTGCTGTTCATCT
GATGCTTTTGAAAGAATAGACTCATTTAGTGTACAGTCATTAGATAGCCGGAGTGTAAGT
GAAATCAATTCAGATGATGAATTGTCAGGCAAGGGATATGCTTTAGTGCCTATTATAGTT
AATTCTTCAACTCCAAAGTCTAAAACAGTTGAATCTGCTGAAGGAAAATCTGAAGAAGTA
AATGAAACATTAGTTATACCCACTGAGGAAGCAGAAATGGAAGAAAGTGGACGAAGTGCA
ACTCCTGTTAACTGTGAACAGCCTGATATCTTGGTTTCTTCCACACCAATAAATGAAGGA
CAGACTGTGTTAGACAAGGTGGCTGAGCAGTGTGAACCTGCTGAAAGTCAGCCAGAAGCA
CTTTCTGAGAAGGAAGATGTTTGCAAGACAGTTGAATTTCTGAATGAAAAGCTGGAAAAA
AGGGAGGCTCAGTTATTATCTCTTAGTAAGGAAAAAGCACTTCTAGAAGAAGCTTTTGAT
AACCTGAAAGATGAAATGTTCAGAGTGAAAGAAGAAAGCAGTAGCATTTCTTCCTTGAAA
GATGAGTTTACTCAAAGAATTGCAGAAGCAGAAAAGAAAGTTCAACTAGCCTGCAAAGAG
AGAGATGCTGCTAAAAAGGAAATCAAAAACATAAAAGAAGAACTTGCCACTAGATTAAAT
AGTAGTGAAACTGCAGACCTTTTGAAAGAGAAAGATGAGCAGATCCGAGGGTTAATGGAA
GAAGGAGAAAAACTTTCAAAACAGCAGCTGCACAATTCTAACATCATCAAGAAATTAAGA
GCTAAAGACAAGGAGAATGAAAATATGGTTGCAAAGCTGAACAAAAAAGTTAAAGAGCTA
GAAGAGGAGTTGCAGCATTTGAAACAGGTCCTTGATGGCAAAGAAGAGGTTGAGAAACAA
CATAGAGAAAATATTAAAAAACTAAATTCCATGGTAGAACGCCAAGAGAAAGATCTTGGC
CGTCTTCAGGTAGACATGGATGAACTTGAAGAAAAGAACCGAAGTATTCAGGCTGCCCTG
GATAGTGCATACAAAGAACTTACTGATCTTCACAAAGCCAATGCTGCAAAGGATAGTGAG
GCACAGGAAGCTGCTCTGAGCCGTGAAATGAAAGCTAAAGAAGAACTTTCTGCAGCATTA
GAGAAGGCCCAAGAAGAAGCCCGTCAGCAGCAAGAAACATTAGCCATTCAAGTGGGGGAC
CTTAGGCTTGCATTGCAGCGTACAGAACAAGCGGCTGCCAGAAAGGAGGATTATTTACGC
CATGAGATCGGTGAACTTCAGCAGAGACTCCAGGAAGCAGAGAATCGAAACCAGGAACTG
AGTCAAAGTGTTTCATCAACAACAAGACCATTGCTTCGACAAATAGAAAATTTGCAAGCA
ACCCTGGGATCCCAGACATCGTCGTGGGAGAAATTAGAGAAGAATCTTTCTGATAGGCTT
GGTGAATCCCAGACCTTGCTGGCAGCAGCAGTTGAGAGAGAACGTGCAGCTACAGAAGAA
CTCCTTGCTAACAAAATTCAGATGTCTTCCATGGAGTCACAGAATTCTCTTTTAAGACAG
GAAAACAGTAGATTTCAAGCCCAGCTAGAATCAGAGAAAAATAGGCTGTGTAAACTGGAG
GATGAGAACAATAGGTACCAGGTTGAATTGGAAAACCTAAAAGATGAATATGTAAGAACA
CTTGAAGAGACGAGGAAAGAAAAGACATTGTTGAATAGTCAGTTAGAAATGGAAAGAATG
AAAGTTGAACAAGAAAGGAAGAAAGCCATTTTTACTCAAGAAACAATAAAAGAAAAGGAA
CGCAAGCCATTTTCTGTTTCTAGCACTCCCACCATGTCACGCTCAAGTTCAATAAGTGGT
GTTGATATGGCAGGACTACAGACATCTTTTCTGTCTCAGGATGAGTCTCATGATCACTCA
TTTGGACCAATGCCTATATCAGCAAATGGAAGCAATCTTTATGATGCTGTAAGGATGGGA
GCAGGATCAAGCATAATTGAAAACCTACAGTCTCAGCTAAAGCTAAGGGAAGGGGAAATC
ACTCATTTACAGCTAGAAATTGGCAATCTAGAAAAAACTCGATCAATAATGGCTGAAGAA
CTAGTTAAATTAACAAATCAAAATGATGAACTTGAAGAGAAGGTGAAGGAGATACCCAAA
CTTAGAACTCAGCTAAGAGATTTGGATCAAAGGTACAACACTATTCTGCAGATGTATGGA
GAAAAAGCAGAAGAGGCAGAAGAACTTCGATTAGATCTCGAAGATGTAAAAAATATGTAC
AAAACTCAAATAGATGAACTTTTAAGACAAAGTCTCAGTTTG
Protein sequence predicted based on nucleotide sequence
MSWFNASQLSSFAKQALSQAQKSIDRVLDIQEEEPSIWAETIPYGEPGISSPVSGGWDTS TWGLKSNTEPQSPPIASPKAITKPVRRTVVDESENFFSAFLSPTDVQTIQKSPVVSKPPA KSQRPEEEVKSSLHESLHIGQSRTPETTESQVKDSSLCVSGETLAAGTSSPKTEGKHEET VNKESDMKVPTVSLKVSESVIDVKTTMESISNTSTQSLTAETKDIALEPKEQKHEDRQSN TPSPPVSTFSSGTSTTSDIEVLDHESVISESSASSRQETTDSKSSLHLMQTSFQLLSASA CPEYNRLDDFQKLTESCCSSDAFERIDSFSVQSLDSRSVSEINSDDELSGKGYALVPIIV NSSTPKSKTVESAEGKSEEVNETLVIPTEEAEMEESGRSATPVNCEQPDILVSSTPINEG QTVLDKVAEQCEPAESQPEALSEKEDVCKTVEFLNEKLEKREAQLLSLSKEKALLEEAFD NLKDEMFRVKEESSSISSLKDEFTQRIAEAEKKVQLACKERDAAKKEIKNIKEELATRLN SSETADLLKEKDEQIRGLMEEGEKLSKQQLHNSNIIKKLRAKDKENENMVAKLNKKVKEL EEELQHLKQVLDGKEEVEKQHRENIKKLNSMVERQEKDLGRLQVDMDELEEKNRSIQAAL DSAYKELTDLHKANAAKDSEAQEAALSREMKAKEELSAALEKAQEEARQQQETLAIQVGD LRLALQRTEQAAARKEDYLRHEIGELQQRLQEAENRNQELSQSVSSTTRPLLRQIENLQA TLGSQTSSWEKLEKNLSDRLGESQTLLAAAVERERAATEELLANKIQMSSMESQNSLLRQ ENSRFQAQLESEKNRLCKLEDENNRYQVELENLKDEYVRTLEETRKEKTLLNSQLEMERM KVEQERKKAIFTQETIKEKERKPFSVSSTPTMSRSSSISGVDMAGLQTSFLSQDESHDHS FGPMPISANGSNLYDAVRMGAGSSIIENLQSQLKLREGEITHLQLEIGNLEKTRSIMAEE LVKLTNQNDELEEKVKEIPKLRTQLRDLDQRYNTILQMYGEKAEEAEELRLDLEDVKNMY KTQIDELLRQSLSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
