Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00863186
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3300nts
Open reading frame: 1 to 3300
ATGGATCACACAGGGGCGATAGACACCGAGGATGAAGTGGGACCTTTAGCCCATCTTGCT
CCAAGTCCTCAGAGTGAAGCTGTGGCTCATGAATTCCAGGAACTCTCCTTGCAGTCCAGT
CAAAACTTACCCCCTCTGAACGAAAGGAAAAATGTGCTCCAGCTGAGGCTGCAACAAAGG
AGGACGAGAGAACAACTAGTGGACCAGGGCATCATGCCACCTTTGAAGAGCCCAGCGGCA
TTCCATGAACAGATAAAAAGCTTGGAACGAGCCAGAACTGAAAACTTTTTGAAACACAAG
ATTCGGAGTCGACCAGATCGTTCTGAACTTGTCAGGATGCACATTTTAGAAGAAACATTT
GCAGAGCCATCCCTGCAGGCTACTCAGATGAAGTTGAAAAGAGCTCGACTAGCAGATGAT
CTGAATGAAAAGATTGCTCAAAGACCTGGTCCTATGGAGCTGGTAGAGAAAAACATCCTT
CCTGTGGACTCCAGTGTTAAAGAAGCAATTATAGGCGTTGGGAAGGAGGACTATCCCCAC
ACTCAGGGCGATTTCTCATTTGATGAAGACAGCAGTGACGCTTTGTCTCCGGACCAGCCT
GCGAGTCAGGAGTCACAGGGGTCAGCCGCGTCCCCAAGTGAGCCAAAAGTTAGTGAATCG
CCATCTCCTGTGACTACAAACACTCCAGCGCAGTTTGCTTCAGTGTCCCCAACAGTTCCT
GAATTCTTGAAAACTCCTCCAACTGCAGATCAGCCTCCCCCACGGCCTGCAGCTCCTGTC
CTCCCCACAAACACTGTGTCCTCAGCAAAGCCTGGCCCAGCACTGGTGAAGCAAAGCCAT
CCCAAGAATCCAAATGACAAACACCGTAGCAAAAAGTGCAAAGATCCCAAACCACGGGTA
AAGAAGTTAAAGTACCACCAATACATTCCACCAGATCAGAAGGGTGAGAAGAATGAGCCG
CAGATGGACTCTAACTACGCCCGCCTGCTCCAGCAGCAGCAGCTGTTCCTGCAACTGCAG
ATCCTGAGTCAGCAGAAGCAGCACTACAACTACCAGACCATCCTGCCTGCACCATTCAAG
CCACTCAATGACAAAAATAGTAACAGTGGGAATTCAGCTTTGAACAATGCCACACCTAAC
ACACCAAGACAGAATACATCTACTCCTGTGAGAAAGCCAGGACCTCTGCCTTCTAGCCTG
GATGACTTAAAGGTATCAGAACTGAAGACAGAACTGAAGTTAAGGGGTCTGCCAGTGTCA
GGCACCAAACCGGACCTCATTGAGCGCCTAAAACCCTACCAGGAAGTGAACAGCAGCGGC
CTTGCTGCTGGGGGCATCGTGGCAGTGTCATCATCAGCCATTGTCACCAGTAACCCAGAA
GTCACTGTGGCCTTGCCGGTTACAACACTACACAACACTGTGACTAGCTCAGTCTCTACT
CTCAAGGCAGAATTGCCACCTACAGGAACCAGCAACGCAACCCGTGTGGAAAATGTTCAT
TCCCCTCTGCCCATTTCACCATCTCCCTCCGAACAGTCCAGTCTCAGTACTGATGACACA
AACATGGCAGACACTTTCACCGAGATTATGACCATGATGTCGCCTTCACAGTTCTTGAGT
TCATCTCCTTTGAGAATGACAAATAATGAAGACAGTCTGAGTCCCACCAGCAGCACTCTG
TCAAACCTGGAACTGGATGCAGCCGAAAAGGATCGCAAGCTTCAGGAGAAAGAGAAGCAA
ATCGAAGAGCTGAAGAGGAAACTGGAACAAGAGCAGAAGCTCGTGGAAGTGCTGAAAATG
CAACTTGAGGTTGAAAAACGAGGGCAGCAGCAGCGGCCCCTGGAAGCCCAGCCCAGTGCC
CCAGGTCATTCTGTCAAGTCAGATCAGAAGCACGGCAGCCTTGGCTCCTCCATCAAAGAT
GAGGCCTCACTCCCTGACTGCTCCAGCTCCAGGCAGCCCATCCCAGTAGCCAGCCACGCT
GTAGGCCAGCCCGTCTCTACAGGTGGCCAGACCCTTGTTGCCAAAAAGGCTGTAGTTATC
AAGCAAGAGGTCCCTGTGGGCCAGGCAGAGCAGCAGAGTGTCGTCTCGCAGTTTTATGTG
AGTTCCCAGGGACAGCCACCGCCTGCTGTTGTTGCTCAGCCCCAGGCTTTACTGACCACG
CAGACTGCTCAGCTGCTGCTCCCAGTGTCCATCCAGGGCTCGAGTGTCACCTCAGTGCAA
CTCCCTGTAGGCAGCCTCAAACTCCAGACTTCACCACAAGCAGGAATGCAGACTCAGCCT
CAGATAGCAACTGCTGCACAAATACCAACTGCTGCCTTGGCCTCAGGCTTGGCCCCAACT
GTACCTCAGACACAAGACACGTTCCCGCAGCATGTGCTCAGTCAGCCTCAACAAGTCAGA
AAGGTTTTCACAAACTCAGCATCATCAAATACAGTTCTTCCATATCAGAGACATCCTGCC
CCAGCTGTCCAGCAGCCCTTTATCAATAAGGCCTCCAACAGTGTTCTTCAATCCAGAAAT
GCTCCGCTTCCATCCCTGCAAAATGGACCTAACACACCCAACAAGCCTAGTTCACCCCCG
CCACCCCAGCAATTTGTCGTCCAGCACTCTCTATTTGGGAGTCCAGTCGCCAAGACAAAA
GATCCCCCCCGCTATGAGGAGGCCATCAAGCAGACACGCAGCACACAGGCCCCTCTGCCA
GAGATTTCCAACGCTCACAGTCAGCAGATGGATGACCTCTTTGATATCCTCATTAAGAGT
GGAGAGATCTCCCTCCCCATAAAAGAAGAACCTTCTCCTATTTCCAAAATGAGACCAGTG
ACAGCCAGCATCACCACAATGCCAGTGAATACAGTGGTGTCCCGGCCACCACCCCAAGTC
CAAATGGCACCACCTGTATCTTTAGAACCTATGGGCAGTTTATCTGCCAGCTTAGAGAAC
CAACTAGAAGCTTTCTTGGATGGAACTTTACCCTCAGCCAATGAAATTCCTCCACTACAA
AGCAGCAGTGAAGACAGAGAGCCCTTCTCTCTGATCGAGGACCTCCAGAATGATCTGCTG
AGTCACTCAGGTATGCTGGACCATTCACACTCACCCATGGAGACTTCCGAGACCCAGTTT
GCTGCAGGTACTCCCTGTCTGTCTCTCGACCTGTCAGACTCAAACTTGGACAACATGGAG
TGGTTGGACATTACCATGCCCAACTCCTCTTCAGGACTCACTCCTCTCAGCACCACCGCG
CCGAGCATGTTCTCTGCTGACTTTCTAGACCCACAGGACCTACCGCTGCCATGGGACTTG
Protein sequence predicted based on nucleotide sequence
MDHTGAIDTEDEVGPLAHLAPSPQSEAVAHEFQELSLQSSQNLPPLNERKNVLQLRLQQR RTREQLVDQGIMPPLKSPAAFHEQIKSLERARTENFLKHKIRSRPDRSELVRMHILEETF AEPSLQATQMKLKRARLADDLNEKIAQRPGPMELVEKNILPVDSSVKEAIIGVGKEDYPH TQGDFSFDEDSSDALSPDQPASQESQGSAASPSEPKVSESPSPVTTNTPAQFASVSPTVP EFLKTPPTADQPPPRPAAPVLPTNTVSSAKPGPALVKQSHPKNPNDKHRSKKCKDPKPRV KKLKYHQYIPPDQKGEKNEPQMDSNYARLLQQQQLFLQLQILSQQKQHYNYQTILPAPFK PLNDKNSNSGNSALNNATPNTPRQNTSTPVRKPGPLPSSLDDLKVSELKTELKLRGLPVS GTKPDLIERLKPYQEVNSSGLAAGGIVAVSSSAIVTSNPEVTVALPVTTLHNTVTSSVST LKAELPPTGTSNATRVENVHSPLPISPSPSEQSSLSTDDTNMADTFTEIMTMMSPSQFLS SSPLRMTNNEDSLSPTSSTLSNLELDAAEKDRKLQEKEKQIEELKRKLEQEQKLVEVLKM QLEVEKRGQQQRPLEAQPSAPGHSVKSDQKHGSLGSSIKDEASLPDCSSSRQPIPVASHA VGQPVSTGGQTLVAKKAVVIKQEVPVGQAEQQSVVSQFYVSSQGQPPPAVVAQPQALLTT QTAQLLLPVSIQGSSVTSVQLPVGSLKLQTSPQAGMQTQPQIATAAQIPTAALASGLAPT VPQTQDTFPQHVLSQPQQVRKVFTNSASSNTVLPYQRHPAPAVQQPFINKASNSVLQSRN APLPSLQNGPNTPNKPSSPPPPQQFVVQHSLFGSPVAKTKDPPRYEEAIKQTRSTQAPLP EISNAHSQQMDDLFDILIKSGEISLPIKEEPSPISKMRPVTASITTMPVNTVVSRPPPQV QMAPPVSLEPMGSLSASLENQLEAFLDGTLPSANEIPPLQSSSEDREPFSLIEDLQNDLL SHSGMLDHSHSPMETSETQFAAGTPCLSLDLSDSNLDNMEWLDITMPNSSSGLTPLSTTA PSMFSADFLDPQDLPLPWDL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
