Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00863211
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4875nts
Open reading frame: 1 to 4875
ATGAGCAAGAGACGCATGAGCGTGGGTCAGCAAACATGGGCTCTTCTCTGCAAGAACTGT
CTCAAAAAATGGAGAATGAAAAGACAGACCTTGTTGGAATGGCTCTTTTCATTTCTTCTG
GTACTGTTTCTGTACCTATTTTTCTCCAATTTACATCAAGTTCATGACACTCCTCAAATG
TCTTCAATGGATCTGGGACGTGTAGATAGTTTTAATGATACTAATTATGTTATTGCATTT
GCACCTGAATCCAAAACTACCCAAGAGATAATGAACAAAGTGGCTTCAGCCCCATTCCTA
AAAGGAAGAACAATCATGGGGTGGCCTGATGAAAAAAGCATGGATGAATTGGATTTGAAC
TATTCAATAGACGCAGTGAGAGTCATCTTTACTGATACCTTCTCCTACCATTTGAAGTTT
TCTTGGGGACATAGAATCCCCATGATGAAAGAGCACAGAGACCATTCAGCTCACTGTCAA
GCAGTGAATGAAAAAATGAAGTGTGAAGGTTCAGAGTTCTGGGAGAAAGGCTTTGTAGCT
TTTCAAGCTGCCATTAATGCTGCTATCATAGAAATCGCAACAAATCATTCAGTGATGGAA
CAGCTGATGTCAGTTACTGGTGTACATATGAAGATATTACCTTTTGTTGCCCAAGGAGGA
GTTGCAACTGATTTTTTCATTTTCTTTTGCATTATTTCTTTTTCTACATTTATATACTAT
GTATCAGTCAATGTTACACAAGAAAGACAATACATTACGTCATTGATGACAATGATGGGA
CTCCGAGAGTCAGCATTCTGGCTTTCCTGGGGTTTGATGTATGCTGGCTTCATCCTTATC
ATGGCCACTTTAATGGCTCTTATTGTAAAATCTGCACAAATTGTCGTCCTGACTGGTTTT
GTGATGGTCTTCACCCTCTTTCTCCTCTATGGCCTGTCTTTGATAACTTTAGCTTTCCTG
ATGAGTGTGTTGATAAAGAAACCTTTCCTTACGGGCTTGGTTGTGTTTCTCCTTATTGTC
TTTTGGGGGATCCTGGGATTCCCAGCATTGTATACACGTCTTCCTGCATTTTTGGAATGG
ACTTTGTGTCTTCTTAGCCCCTTTGCCTTCACTGTTGGGATGGCCCAGCTTATACATTTG
GACTATGATGTGAATTCTAATGCCCACTTGGATTCTTCACAAAATCCATACCTCATAATA
GCTACTCTTTTCATGTTGGTTTTTGACACCCTTCTGTATTTGGTATTGACATTATATTTT
GACAAAATTTTGCCCGCTGAATATGGACATCGATGTTCTCCCTTGTTTTTCCTGAAATCC
TGTTTTTGGTTTCAACACGGAAGGGCTAATCATGTGGTCCTTGAGAATGAAACAGATTCT
GATCCTACACCTAATGACTGTTTTGAACCAGTGTCTCCAGAATTCTGTGGGAAAGAAGCC
ATCAGAATCAAAAATCTTAAAAAAGAATATGCAGGGAAGTGTGAGAGAGTAGAAGCTTTG
AAAGGTGTGGTGTTTGACATATATGAAGGCCAGATCACTGCCCTCCTTGGTCACAGTGGA
GCTGGAAAAACTACCCTGTTAAACATACTTAGTGGGTTGTCAGTTCCAACATCAGGTTCA
GTCACTGTCTATAATCACACACTTTCAAGAATGGCTGATATAGAAAATATCAGCAAGTTC
ACTGGATTTTGTCCACAATCCAATGTGCAATTTGGATTTCTCACTGTGAAAGAAAACCTC
AGGCTGTTTGCTAAAATAAAAGGGATTTTGCCACATGAAGTGGAGAAAGAGGTACAACGA
GTTGTACAGGAATTAGAAATGGAAAATATTCAAGACATCCTTGCTCAAAACTTAAGTGGT
GGACAAAATAGGAAACTAACTTTTGGGATTGCCATTTTAGGAGATCCTCAAGTTTTGCTA
TTGGATGAACCGACTGCTGGATTGGATCCTCTTTCAAGGCACCGAATATGGAATCTCCTG
AAAGAGGGGAAATCAGACAGAGTAATTCTCTTCAGCACCCAGTTTATAGATGAGGCTGAC
ATTCTGGCGGACAGGAAGGTGTTCATATCCAATGGGAAGCTGAAGTGTGCAGGCTCTTCT
CTGTTCCTTAAGAAGAAATGGGGCATAGGCTACCATTTAAGTTTGCATCTGAATGAAAGG
TGTGATCCAGAGAGTATAACATCACTGGTTAAGCAGCACATCTCTGATGCCAAATTGACA
GCACAAAGTGAAGAAAAACTTGTATATATTTTGCCTTTGGAAAGGACAAACAAATTTCCA
GAACTTTACAGGGATCTTGATAGATGTTCTAACCAAGGCATTGAGGATTATGGTGTTTCC
ATAACAACTTTGAATGAGGTGTTTCTGAAATTAGAAGGAAAATCAACTATTGATGAATCA
GATATTGGAATTTGGGGACAATTACAAACTGATGGGGCAAAAGATATAGGAAGCCTTGTT
GAGCTGGAACAAGTTTTGTCTTCCTTCCACGAAACAAGGAAAACAATCAGTGGCGTGGCG
CTCTGGAGGCAGCAGGTCTGTGCAATAGCAAAAGTTCGCTTCCTAAAGTTAAAGAAAGAA
AGAAAAAGCCTGTGGACTATATTATTGCTTTTTGGTATTAGCTTTATCCCTCAACTTTTG
GAACATCTATTCTACGAGTCATATCAGAAAAGTTACCCGTGGGAACTGTCTCCAAATACA
TACTTCCTCTCACCAGGACAACAACCACAGGATCCTCTGACCCATTTACTGGTCATCAAT
AAGACAGGGTCAACCATTGATAACTTTTTACATTCACTGAGGCGACAGAACATAGCTATA
GAAGTGGATGCCTTTGGAACTAGAAATGGCACAGATGACCCATCTTACAATGGTGCTATC
ATTGTGTCAGGTGATGAAAAGGATCACAGATTTTCAATAGCATGTAATACAAAACGGCTG
AATTGCTTTCCTGTCCTCCTGGATGTCATTAGCAATGGACTACTTGGAATTTTTAATTCG
TCAGAACACATTCAGACTGACAGAAGCACATTTTTTGAAGAGCATATGGATTATGAGTAT
GGGTACCGAAGTAACACCTTCTTCTGGATACCGATGGCAGCCTCTTTCACTCCATACATT
GCAATGAGCAGCATTGGTGACTACAAAAAAAAAGCTCATTCCCAGCTACGGATTTCAGGC
CTCTACCCTTCTGCATACTGGTTTGGCCAAGCACTGGTGGATGTTTCCCTGTACTTTTTG
ATCCTCCTGCTAATGCAAATAATGGATTATATTTTTAGCCCAGAGGAGATTATATTTATA
ATTCAAAACCTGTTAATTCAAATCCTGTGTAGTATTGGCTATGTCTCATCTCTTGTTTTC
TTGACATATGTGATTTCATTCATTTTTCGCAATGGGAGAAAAAATAGTGGCATTTGGTCA
TTTTTCTTCTTAATTGTGGTCATCTTCTCGATAGTTGCTACTGATCTAAATGAATATGGA
TTTCTAGGGCTATTTTTTGGCACCATGTTAATACCTCCCTTCACATTGATTGGCTCTCTA
TTCATTTTTTCTGAGATTTCTCCTGATTCCATGGATTACTTAGGAGCTTCAGAATCTGAA
ATTGTATACCTGGCACTGCTAATACCTTACCTTCATTTTCTCATTTTTCTTTTCATTCTG
CGATGCCTAGAAATGAACTGCAGGAAGAAACTAATGAGAAAGGATCCTGTGTTCAGAATT
TCTCCAAGAAGCAACGCTATTTTTCCAAACCCAGAAGAGCCTGAAGGAGAGGAGGAAGAT
ATCCAGATGGAAAGAATGAGAACAGTGAATGCTATGGCTGTGCGAGACTTTGATGAGACA
CCCGTCATCATTGCCAGCTGTCTACGGAAGGAATATGCAGGCAAAAAGAAAAATTGCTTT
TCTAAAAGGAAGAAAAAAATTGCCACAAGAAATGTCTCTTTTTGTGTTAAAAAAGGTGAA
GTTATAGGACTGTTAGGACACAATGGAGCTGGTAAAAGTACAACTATTAAGATGATAACT
GGAGACACAAAACCAACTGCAGGACAGGTGATTTTGAAAGGGAGCGGTGGAGGGGAACCC
CTGGGCTTCCTGGGGTACTGCCCTCAGGAGAATGCGCTGTGGCCCAACCTGACAGTGAGG
CAGCACCTGGAGGTGTACGCTGCCGTGAAAGGTCTCAGGAAAGGGGACGCAATGATCGCC
ATCACACGGTTAGTGGATGCGCTCAAGCTGCAGGACCAGCTGAAGGCTCCCGTGAAGACC
TTGTCAGAGGGAATAAAGCGAAAGCTGTGCTTTGTGCTGAGCATCCTGGGGAACCCGTCA
GTGGTGCTTCTGGATGAGCCGTCGACCGGGATGGACCCCGAGGGGCAGCAGCAAATGTGG
CAGGTGATTCGGGCCACCTTTAGAAACACGGAGAGGGGCGCCCTCCTGACCACCCACTAC
ATGGCAGAGGCTGAGGCGGTGTGTGACCGAGTGGCCATCATGGTGTCAGGAAGGCTGAGA
TGTATTGGTTCCATCCAACACCTGAAAAGCAAATTTGGCAAAGACTACCTGCTGGAGATG
AAGCTGAAGAACCTGGCACAAATGGAGCCCCTCCATGCAGAGATCCTGAGGCTTTTCCCC
CAGGCTGCTCAGCAGGAAAGGTTCTCCTCCCTGATGGTCTATAAGTTGCCTGTTGAGGAT
GTGCGACCTTTATCACAGGCTTTCTTCAAATTAGAGATAGTTAAACAGAGTTTCGACCTG
GAGGAGTACAGCCTCTCACAGTCTACCCTGGAGCAGGTTTTCCTGGAGCTCTCCAAGGAG
CAGGAGCTGGGTGATCTTGAAGAGGACTTTGATCCCTCGGTGAAGTGGAAACTCCTCCTG
CAGGAAGAGCCTTTG
Protein sequence predicted based on nucleotide sequence
MSKRRMSVGQQTWALLCKNCLKKWRMKRQTLLEWLFSFLLVLFLYLFFSNLHQVHDTPQM SSMDLGRVDSFNDTNYVIAFAPESKTTQEIMNKVASAPFLKGRTIMGWPDEKSMDELDLN YSIDAVRVIFTDTFSYHLKFSWGHRIPMMKEHRDHSAHCQAVNEKMKCEGSEFWEKGFVA FQAAINAAIIEIATNHSVMEQLMSVTGVHMKILPFVAQGGVATDFFIFFCIISFSTFIYY VSVNVTQERQYITSLMTMMGLRESAFWLSWGLMYAGFILIMATLMALIVKSAQIVVLTGF VMVFTLFLLYGLSLITLAFLMSVLIKKPFLTGLVVFLLIVFWGILGFPALYTRLPAFLEW TLCLLSPFAFTVGMAQLIHLDYDVNSNAHLDSSQNPYLIIATLFMLVFDTLLYLVLTLYF DKILPAEYGHRCSPLFFLKSCFWFQHGRANHVVLENETDSDPTPNDCFEPVSPEFCGKEA IRIKNLKKEYAGKCERVEALKGVVFDIYEGQITALLGHSGAGKTTLLNILSGLSVPTSGS VTVYNHTLSRMADIENISKFTGFCPQSNVQFGFLTVKENLRLFAKIKGILPHEVEKEVQR VVQELEMENIQDILAQNLSGGQNRKLTFGIAILGDPQVLLLDEPTAGLDPLSRHRIWNLL KEGKSDRVILFSTQFIDEADILADRKVFISNGKLKCAGSSLFLKKKWGIGYHLSLHLNER CDPESITSLVKQHISDAKLTAQSEEKLVYILPLERTNKFPELYRDLDRCSNQGIEDYGVS ITTLNEVFLKLEGKSTIDESDIGIWGQLQTDGAKDIGSLVELEQVLSSFHETRKTISGVA LWRQQVCAIAKVRFLKLKKERKSLWTILLLFGISFIPQLLEHLFYESYQKSYPWELSPNT YFLSPGQQPQDPLTHLLVINKTGSTIDNFLHSLRRQNIAIEVDAFGTRNGTDDPSYNGAI IVSGDEKDHRFSIACNTKRLNCFPVLLDVISNGLLGIFNSSEHIQTDRSTFFEEHMDYEY GYRSNTFFWIPMAASFTPYIAMSSIGDYKKKAHSQLRISGLYPSAYWFGQALVDVSLYFL ILLLMQIMDYIFSPEEIIFIIQNLLIQILCSIGYVSSLVFLTYVISFIFRNGRKNSGIWS FFFLIVVIFSIVATDLNEYGFLGLFFGTMLIPPFTLIGSLFIFSEISPDSMDYLGASESE IVYLALLIPYLHFLIFLFILRCLEMNCRKKLMRKDPVFRISPRSNAIFPNPEEPEGEEED IQMERMRTVNAMAVRDFDETPVIIASCLRKEYAGKKKNCFSKRKKKIATRNVSFCVKKGE VIGLLGHNGAGKSTTIKMITGDTKPTAGQVILKGSGGGEPLGFLGYCPQENALWPNLTVR QHLEVYAAVKGLRKGDAMIAITRLVDALKLQDQLKAPVKTLSEGIKRKLCFVLSILGNPS VVLLDEPSTGMDPEGQQQMWQVIRATFRNTERGALLTTHYMAEAEAVCDRVAIMVSGRLR CIGSIQHLKSKFGKDYLLEMKLKNLAQMEPLHAEILRLFPQAAQQERFSSLMVYKLPVED VRPLSQAFFKLEIVKQSFDLEEYSLSQSTLEQVFLELSKEQELGDLEEDFDPSVKWKLLL QEEPL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
