Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00863754
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5817nts
Open reading frame: 1 to 5817
ATGAGCTCTGACGCAGAAATGGCCATTTTTGGAGAAGCAGCTCCCTACCTCCGGAAACCA
GAGAAGGAGAGAATCGAGGCTCAAAATCGTCCATTCGATTCCAAGAAAGCCTGCTTTGTA
GCGGATAATAAGGAAATGTATGTGAAAGGCATGATCCAGACTAGGGAAAATGACAAAGTC
ATAGTCAAGACCCTCGATGACCGGATGCTCACTCTGAACAATGACCAGGTCTTCCCCATG
AACCCTCCCAAATTTGACAAGATCGAGGACATGGCCATGATGACTCACCTGCATGAACCT
GCTGTTCTGTACAACCTCAAAGAGCGCTATGCAGCCTGGATGATCTACACCTACTCAGGC
CTCTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAAGCCCGAGGTGGTG
GCTGCCTACAGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGAC
AATGCCTATCAGTTCATGCTGACTGATCGAGACAACCAGTCTATCCTCATCACCGGAGAA
TCCGGGGCTGGGAAGACTGTGAACACCAAGCGTGTCATCCAGTATTTTGCAACAATTGCA
GTTACCGGGGACAAGAAGAAGGAGACACAGCCAGGCAAAATGCAGGGAACCCTAGAGGAT
CAGATCATCCAGGCCAACCCACTGCTGGAGGCCTTTGGAAATGCCAAGACTGTGAGGAAT
GACAACTCCTCAAGATTTGGGAAGTTCATTCGGATTCATTTTGGAGCCACAGGAAAGCTG
GCATCGGCAGACATCGAAACTTATCTGTTAGAAAAATCCAGAGTGACGTTTCAATTATCC
AGTGAGAGAAGCTATCATATTTTCTACCAAATTATGTCAAACAAGAAGCCAGAACTAATT
GACCTGCTTCTGATCTCCACCAACCCCTTCGACTTCCCCTTCGTGAGCCAAGGAGAGGTC
ACGGTAGCCAGTATCGATGACAGTGAAGAACTGCTGGCGACAGATAATGCCATTGACATC
CTGGGCTTCAGCTCAGAGGAGAAAGTCGGGATCTACAAACTGACGGGAGCCGTGATGCAT
TATGGGAACATGAAGTTCAAGCAGAAGCAGCGTGAGGAGCAGGCGGAGCCAGACGGCACC
GAAGTGGCTGACAAAGCCGGATACCTGATGGGACTGAATTCTGCAGAAATGCTGAAGGGC
CTGTGCTGTCCAAGGGTGAAGGTTGGCAATGAATATGTCACTAAAGGGCAAAATGTCCAG
CAGGTGACCAATTCGGTGGGTGCTCTGGCCAAAGCCGTCTACGAGAAGATGTTCCTGTGG
ATGGTCACCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATCGGG
GTCTTGGACATTGCTGGCTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATC
AACTTCACCAATGAGAAACTGCAACAGTTTTTCAACCACCACATGTTCGTGCTGGAGCAG
GAAGAGTACAAGAAGGAAGGCATCGAGTGGGAGTTCATTGACTTCGGAATGGACCTGGCT
GCCTGCATCGAGCTCATCGAGAAGCCTATGGGCATCTTCTCCATCCTGGAAGAGGAGTGC
ATGTTCCCCAAGGCAACAGACACCTCCTTCAAGAACAAGCTGTATGACCAGCATCTTGGA
AAATCCAACAACTTCCAGAAGCCCAAGCCTGCCAAAGGCAAGGCTGAGGCTCACTTCTCG
CTGGTGCACTATGCCGGCACCGTGGACTACAACATCGCCGGCTGGCTGGACAAAAACAAG
GACCCCCTGAACGAGACTGTGGTGGGGCTGTACCAGAAGTCTTCGCTGAAGCTTCTCTCC
TTCCTTTTTTCCAACTATGCTGGTGCAGAGACAGGCGACTCCGGAGGAAGCAAGAAGGGC
GGGAAGAAGAAGGGCTCCTCTTTCCAGACCGTGTCGGCCGTGTTCAGGGAAAATTTAAAC
AAATTGATGACTAACTTAAGGAGCACCCACCCTCACTTTGTACGATGTCTGATTCCCAAT
GAGACCAAGACTCCTGGTGTGATGGACCACTACTTGGTCATGCACCAGCTGCGCTGTAAC
GGGGTCCTCGAGGGCATCCGGATTTGCAGGAAGGGATTCCCCAGCCGGATCCTCTATGCT
GACTTCAAGCAGCGGTACCGGATCCTCAATGCCAGTGCTATCCCTGAAGGGCAGTTCATT
GACAGCAAAAATGCCTCAGAGAAGCTCCTCAACTCCATCGATGTGGACCGGGAGCAGTTC
AGGTTCGGCAACACCAAGGTGTTTTTCAAAGCTGGGCTCCTGGGACTTTTGGAGGAGATG
AGAGATGAGAAGCTGGTGACGCTGATGACAAGCACGCAGGCGGTGTGCAGGGGGTACCTG
ATGCGGGTGGAGTTCAAGAAGATGATGGAGAGGAGGGACTCCATCTTCTGCATCCAGTAC
AACATCCGCTCTTTTATGAACGTCAAGCACTGGCCCTGGATGAACCTGTTCTTCAAAATC
AAGCCCCTGCTGAAGAGTGCAGAGGCCGAGAAGGAGATGGCCACCATGAAGGAAGACTTT
GAGAGGACCAAGGAAGAACTGGCCCGATCTGAGGCTCGCCGGAAGGAGCTGGAGGAGAAA
ATGGTCTCCCTCCTGCAGGAGAAGAATGACCTCCAATTGCAGGTCCAGTCTGAAACAGAA
AATCTGATGGACGCTGAGGAACGGTGTGAAGGACTCATCAAAAGCAAGATCCTACTGGAA
GCAAAAGTCAAGGAGCTGACGGAGAGATTGGAAGAGGAAGAGGAGATGAATTCTGAATTG
GTTGCCAAGAAGAGGAATCTGGAAGATAAATGCTCCTCTCTCAAGAGAGACATTGATGAC
CTGGAGCTGACCTTGACGAAAGTTGAAAAGGAGAAGCATGCCACAGAGAACAAGGTAAAG
AATCTTTCCGAAGAAATGACAGCACTTGAAGAAAACATTTCCAAATTGACCAAAGAAAAG
AAATCTCTACAGGAGGCCCATCAGCAAACACTGGATGATCTTCAGGTGGAAGAAGATAAA
GTCAATGGTCTAATCAAAATAAATGCCAAGCTTGAACAGCAAACAGATGATCTTGAGGGT
TCCTTAGAGCAGGAGAAGAAACTGCGGGCGGACTTGGAAAGGGCGAAGAGGAAGCTGGAA
GGAGATCTGAAAATGTCCCAGGAATCCATTATGGATCTAGAAAATGACAAGCAGCAAATA
GAAGAGAAATTGAAAAAGAAGGAGTTTGAACTCAGTCAGTTACAAGCCAAAATAGATGAC
GAACAAGTCCACAGTTTGCAGTTTCAAAAGAAGATTAAAGAACTGCAAGCCCGCATAGAA
GAGCTGGAGGAGGAAATTGAAGCGGAACACACGCTCAGAGCCAAGATTGAGAAGCAGCGC
TCAGATCTGGCCAGGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCAGTGGGGCC
ACTTCAGCCCAGATTGAGATGAACAAGAAGAGGGAGGCTGAGTTCCAGAAAATGCGCAGG
GACCTGGAGGAGGCCACCCTGCAGCACGAAGCCACAGCAGCCACCCTGAGGAAGAAGCAA
GCAGATAGTGTGGCCGAGCTTGGGGAGCAGATTGACAACCTGCAGCGGGTGAAGCAGAAG
CTGGAGAAGGAGAAGAGCGAGCTGAAGATGGAGATTGACGACATGGCCAGCAACATCGAG
GCTCTCTCCAAGTCAAAGAGTAACATAGAAAGAACGTGCCGGACGGTAGAAGATCAATTT
AGTGAAATCAAAGCCAAGGACGAGCAACAGACACAGTTGATCCATGATCTGAACATGCAG
AAAGCAAGACTGCAGACCCAAAATGGGGAGCTGAGCCACCGAGTGGAAGAGAAGGAGTCT
CTGATTTCACAGCTGACCAAAAGCAAGCAGGCCCTCACCCAGCAGCTGGAGGAGCTTAAG
AGGCAAATGGAAGAAGAAACCAAGGCCAAGAACGCCATGGCGCACGCCCTGCAGTCCTCC
CGCCACGACTGTGACCTGCTGCGGGAACAGTATGAGGAGGAGCAGGAAGCCAAGGCCGAG
CTGCAGAGGGCGCTGTCCAAGGCCAACAGTGAGGTTGCCCAGTGGAGGACCAAATACGAG
ACGGACGCCATTCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAAAAACTGGCCCAGAGG
CTCCAGGAAGCAGAGGAGAACACGGAGACGGCGAACTCCAAGTGCGCATCGTTGGAGAAA
ACCAAGCAGAGGCTGCAGGGAGAGGTGGAGGATCTGATGCGGGATCTGGAGCGCTCCCAC
ACCGCCTGTGCCACACTGGACAAGAAGCAGAGGAACTTCGACAAGGTCCTTGCAGAGTGG
AAGCAAAAGCTGGACGAAAGCCAGGCTGAGTTGGAAGCTGCTCAGAAGGAGTCCAGGTCA
CTCAGCACTGAACTCTTCAAGATGAGGAATGCCTATGAGGAGGTGGTGGACCAGTTAGAG
ACACTGAGGCGAGAGAACAAAAATCTGCAAGAAGAGATTTCCGACTTAACTGAGCAGATT
GCAGAAACTGGCAAGAATCTTCAGGAAGCGGAAAAGACCAAGAAGCTAGTGGAGCAGGAA
AAGTCAGATCTGCAGGTCGCCTTAGAAGAAGTGGAGGGTTCCTTGGAACACGAGGAGAGC
AAGATCTTGCGCGTGCAGCTAGAGCTGAGCCAGGTGAAATCCGAGCTAGACCGCAAGGTC
ATTGAGAAGGATGAAGAAATCGAGCAGCTAAAAAGAAACAGCCAGCGGGCAGCAGAGGCC
CTGCAGAGCGTGCTGGATGCTGAAATCCGCAGCCGGAACGACGCCCTGAGGCTAAAGAAG
AAGATGGAGGGAGACCTTAATGAGATGGAGATTCAGCTGGGCCACTCCAACCGCCAGATG
GCAGAGACCCAGAAGCATCTGCGCACGGTCCAGGGCCAGCTCAAGGACTCCCAGCTGCAT
CTCGATGACGCCCTGAGGAGCAATGAGGACCTCAAGGAGCAGCTGGCCATCGTGGAGCGC
AGGAATGGCCTCCTGCTGGAGGAGCTGGAGGAAATGAAGGTGGCCCTGGAACAGACGGAG
CGGACCCGCAGGCTGTCAGAGCAGGAGCTGCTGGACGCCAGCGACCGCGTGCAGCTCCTG
CACTCCCAGAACACAAGCCTGATAAATACCAAGAAAAAACTGGAGGCTGACATAGCTCAG
TGCCAGGCAGAGGTGGAGAACTCGATCCAGGAGTCCAGGAACGCAGAGGAGAAGGCCAAG
AAGGCCATCACGGATGCTGCCATGATGGCTGAGGAGCTAAAGAAGGAACAGGACACCAGC
GCCCACCTGGAGCGGATGAAGAAGAACCTGGAGCAGACGGTGAAGGACCTGCAGCACCGT
CTAGATGAGGCTGAACAACTGGCGCTGAAGGGCGGGAAGAAGCAGATCCAGAAACTGGAG
AACCGGGTGCGGGAGCTGGAAAATGAGCTTGATGTGGAACAGAAGAGGGGAGCTGAAGCC
CTGAAGGGAGCCCACAAGTACGAACGCAAAGTCAAGGAGATGACTTACCAGGCTGAGGAG
GACCACAAGAATATCCTTAGGCTCCAGGACCTGGTGGACAAGCTGCAGGCCAAAGTGAAG
TCTTACAAGAGGCAGGCTGAGGAGGCGGAGGAGCAGGCCAACACGCAGCTGTCCAGATGC
CGGAGAGTCCAGCATGAGCTAGAGGAGGCCGCGGAGAGGGCGGACATCGCTGAGTCCCAG
GTCAACAAGCTGAGGGCCAAGAGCCGAGACGTGGGCAGCCAGAAGATGGAAGAATTG
Protein sequence predicted based on nucleotide sequence
MSSDAEMAIFGEAAPYLRKPEKERIEAQNRPFDSKKACFVADNKEMYVKGMIQTRENDKV IVKTLDDRMLTLNNDQVFPMNPPKFDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSG LFCVTVNPYKWLPVYKPEVVAAYRGKKRQEAPPHIFSISDNAYQFMLTDRDNQSILITGE SGAGKTVNTKRVIQYFATIAVTGDKKKETQPGKMQGTLEDQIIQANPLLEAFGNAKTVRN DNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLSSERSYHIFYQIMSNKKPELI DLLLISTNPFDFPFVSQGEVTVASIDDSEELLATDNAIDILGFSSEEKVGIYKLTGAVMH YGNMKFKQKQREEQAEPDGTEVADKAGYLMGLNSAEMLKGLCCPRVKVGNEYVTKGQNVQ QVTNSVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCI NFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEEC MFPKATDTSFKNKLYDQHLGKSNNFQKPKPAKGKAEAHFSLVHYAGTVDYNIAGWLDKNK DPLNETVVGLYQKSSLKLLSFLFSNYAGAETGDSGGSKKGGKKKGSSFQTVSAVFRENLN KLMTNLRSTHPHFVRCLIPNETKTPGVMDHYLVMHQLRCNGVLEGIRICRKGFPSRILYA DFKQRYRILNASAIPEGQFIDSKNASEKLLNSIDVDREQFRFGNTKVFFKAGLLGLLEEM RDEKLVTLMTSTQAVCRGYLMRVEFKKMMERRDSIFCIQYNIRSFMNVKHWPWMNLFFKI KPLLKSAEAEKEMATMKEDFERTKEELARSEARRKELEEKMVSLLQEKNDLQLQVQSETE NLMDAEERCEGLIKSKILLEAKVKELTERLEEEEEMNSELVAKKRNLEDKCSSLKRDIDD LELTLTKVEKEKHATENKVKNLSEEMTALEENISKLTKEKKSLQEAHQQTLDDLQVEEDK VNGLIKINAKLEQQTDDLEGSLEQEKKLRADLERAKRKLEGDLKMSQESIMDLENDKQQI EEKLKKKEFELSQLQAKIDDEQVHSLQFQKKIKELQARIEELEEEIEAEHTLRAKIEKQR SDLARELEEISERLEEASGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKQ ADSVAELGEQIDNLQRVKQKLEKEKSELKMEIDDMASNIEALSKSKSNIERTCRTVEDQF SEIKAKDEQQTQLIHDLNMQKARLQTQNGELSHRVEEKESLISQLTKSKQALTQQLEELK RQMEEETKAKNAMAHALQSSRHDCDLLREQYEEEQEAKAELQRALSKANSEVAQWRTKYE TDAIQRTEELEEAKKKLAQRLQEAEENTETANSKCASLEKTKQRLQGEVEDLMRDLERSH TACATLDKKQRNFDKVLAEWKQKLDESQAELEAAQKESRSLSTELFKMRNAYEEVVDQLE TLRRENKNLQEEISDLTEQIAETGKNLQEAEKTKKLVEQEKSDLQVALEEVEGSLEHEES KILRVQLELSQVKSELDRKVIEKDEEIEQLKRNSQRAAEALQSVLDAEIRSRNDALRLKK KMEGDLNEMEIQLGHSNRQMAETQKHLRTVQGQLKDSQLHLDDALRSNEDLKEQLAIVER RNGLLLEELEEMKVALEQTERTRRLSEQELLDASDRVQLLHSQNTSLINTKKKLEADIAQ CQAEVENSIQESRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHR LDEAEQLALKGGKKQIQKLENRVRELENELDVEQKRGAEALKGAHKYERKVKEMTYQAEE DHKNILRLQDLVDKLQAKVKSYKRQAEEAEEQANTQLSRCRRVQHELEEAAERADIAESQ VNKLRAKSRDVGSQKMEEL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
