Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00864024
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3399nts
Open reading frame: 1 to 3399
ATGGAGCTCTTCCAAGCCAAGGACCACTACATCCTGCAGCAGGGCGAGCGCGCGCTGTGG
TGCAGCCGCCGCGACGGCGGCCTCCAGCTCCGACCCGCTACTGATCTACTTCTTGCCTGG
AATCCCATTTGTTTGGGGTTGGTAGAAGGTGTTATTGGGAAAATTCAACTTCATTCAGAT
CTTCCATGGTGGCTTATTCTAATTCGGCAGAAAGCATTGGTGGGCAAACTCCCAGGAGAC
CATGAGGTCTGTAAAGTTACCAAAATTGCTGTGCTCTCACTTTCTGAAATGGAACCTCAG
GATCTTGAGCTAGAGCTCTGTAAGAAGCATCATTTTGGTATTAACAAACCAGAGAAGATC
ATACCATCTCCTGATGACTCAAAGTTTCTACTGAAGACCTTTACGCATATTAAATCCAAT
GTGTCTGCTCCTAATAAAAAGAAAGTTAAGGAAAGTAAAGAGAAGGAGAAGTTGGAGAGG
AGATTACTTGAAGAGTTGCTGAAGATGTTCATGGACTCAGAATCCTTTTATTATAGCTTG
ACCTATGACCTGACCAATTCCGTGCAGAGGCAGAGCACTGGGGAGAGGGACGGTCGGCCC
CTCTGGCAGAAGGTTGATGACCGATTTTTTTGGAATAAATACATGATACAAGATCTTACT
GAGATTGGTACTCCAGATGTGGACTTTTGGATTATCCCCATGATCCAAGGTTTTGTGCAG
ATTGAAGAACTTGTGGTTAATTATACCGAATCATCTGATGATGAGAAAAGCAGCCCAGAG
ACCCCCCCTCAGGAGTCCACCTGTGTAGATGATATTCACCCACGATTTCTAGTGGCTCTC
ATTTCACGCCGAAGTAGGCACAGAGCAGGAATGCGCTATAAACGAAGAGGAGTGGATAAA
AATGGAAATGTTGCCAATTATGTGGAGACTGAGCAGTTGATTCATGTTCATAATCATACC
CTGTCATTTGTTCAAACACGAGGCTCTGTGCCTGTCTTTTGGAGCCAGGTTGGGTATCGA
TATAACCCAAGACCGCGGCTGGACAGAAGTGAAAAGGAAACTGTTGCCTATTTCTGTGCC
CATTTCGAAGAACAACTGAACATTTACAAAAAACAGGTTATTATTAACTTGGTAGACCAG
GCAGGAAGAGAGAAGATTATTGGCGATGCTTACCTGAAGCAAGTGTTGCTTTTCAACAAC
TCACACCTCACTTACGTTTCGTTTGACTTCCATGAGCACTGCCGAGGAATGAAGTTTGAG
AATGTTCAGACACTAACAGATGCCATTTATGACATTATTCTTGATATGAAGTGGTGTTGG
GTTGATGAAGCTGGGGTAATATGTAAGCAGGAAGGGATTTTTCGTGTTAATTGTATGGAC
TGCCTGGATCGCACCAACGTGGTCCAAGCTGCCATCGCGAGAGTGGTCATGGAACAGCAG
CTGAAAAAATTAGGTGTGATGCCCCCGGAACAGCCATTACCTGTGAAATGTAATCGCATC
TACCAGATAATGTGGGCCAATAATGGTGACTCCATTAGCAGACAGTATGCTGGGACAGCT
GCTCTGAAGGGTGACTTTACAAGGACAGGAGAAAGGAAGTTAGCAGGAGTTATGAAAGAT
GGAGTGAACTCAGCAAACAGATATTACCTCAACCGATTTAAGGATGCTTATAGGCAAGCT
GTTATAGATTTGATGCAAGGCATTCCAGTGACAGAAGATCTTTATTCCATATTTACCAAG
GAGAAAGAACATGAAGCTTTGCATAAGGAAAATCAGAGAAGCCACCAGGAACTAATTAGC
CAGCTCTTACAAAGTTACATGAAGTTACTACTGCCTGATGATGAGAAGTTCCATGGGGGC
TGGGCCCTCATTGACTGTGACCCTAGCCTCATTGATGCTACTCACAGAGACGTGGATGTG
CTGTTACTGCTTTCTAACTCTGCCTACTACGTGGCCTATTATGATGATGAAGTTGATAAA
GTAAACCAGTATCAACGACTAAGTCTAGAAAACCTGGAAAAAATTGAAATAGGCCCTGAA
CCCACTCTTTTTGGTAAGCCAAAGTTCTCCTGCATGCGACTGCACTACAGATACAAAGAA
GCGAGTGGCTATTTCCACACATTGCGAGCTGTAATGCGTAATCCTGAAGAGGATGGAAAA
GATACCCTTCAGTGCATTGCAGAGATGCTGCAGATCACCAAGCAAGCCATGGGATCGGAT
TTACCCATAATTGAGAAGAAACTTGAGAGGAAGAGCAGTAAACCTCACGAAGACATCATT
GGTATCAGGTCTCAAAACCAAGGTTCTTTGGCCCAGGGAAAGAATTTTTTAATGAGCAAA
TTTTCATCTCTAAATCAAAAAGTGAAGCAGACCAAATCCAATGTAAATATTGGCAACCTC
CGAAAGCTAGGAAACTTTACCAAACCTGAAATGAAAGTTAACTTTCTAAAACCAAACTTA
AAAGTAAATCTTTGGAAATCAGATAGTAGTCTTGAAACTATGGAAAACACAGGAGTGATG
GATAAGGTTCAGGCAGAGTCTGATGGGGACATGTCTTCAGATAATGACTCATACCACTCT
GATGAATTCCTTACAAATTCTAAGTCTGATGAAGACAGGCAGCTAGCTAACTCATTAGAG
AGTGTAGGGCCAATAGATTACGTTCTTCCTAGTTGTGGTATTATTGCCTCAGCGCCTCGA
TTGGGCAGTCGGTCCCAGTCTCTTAGCAGCACAGATAGTAGCGTTCATGCTCCTTCAGAG
ATTACTGTTGCTCATGGGAGTGGGCTTGGAAAAGGCCAGGAGTCTCCTTTGAAGAAAAGT
CCTTCTGCTGGCGACGTACACATATTGACTGGCTTTGCCAAGCCTATGGATATTTACTGC
CACAGATTTGTGCAAGATGCACAGAACAAAGTGACCCACCTATCAGAGACCAGATCTGTG
TCTCAGCAGGCTAGTCAGGAAAGAAATCAAATGACCAATCAAGTTTCAAATGAAACCCAA
TCAGAATCAACAGAACAGACACCTTCTCGGCCATCGCAATTAGATGTCTCTCTTTCTGCA
ACAGGCCCACAGTTTTTGTCAGTTGAGCCAGCGCATTCAGTTGCATCTCAAAAAACCCCC
ACCTCCGCTTCCAGCATGCTTGAACTTGAGACAGGGCTTCATGTAACTCCTTCTCCTTCA
GAGAGCAGTAGCAGCAGAGCAGTCTCTCCCTTTGCCAAGATTCGAAGTTCCATGGTCCAG
GTTGCTAGTATTACCCAAGCTGGATTAACCCATGGGATAAACTTTGCAGTGTCAAAAGTT
CAGAAGAGTCCTCCAGAACCTGAAATCATTAATCAAGTCCAGCAAAATGAACTTAAAAAG
ATGTTTATACAATGCCAGACACGGATAATTCAGATTTTG
Protein sequence predicted based on nucleotide sequence
MELFQAKDHYILQQGERALWCSRRDGGLQLRPATDLLLAWNPICLGLVEGVIGKIQLHSD LPWWLILIRQKALVGKLPGDHEVCKVTKIAVLSLSEMEPQDLELELCKKHHFGINKPEKI IPSPDDSKFLLKTFTHIKSNVSAPNKKKVKESKEKEKLERRLLEELLKMFMDSESFYYSL TYDLTNSVQRQSTGERDGRPLWQKVDDRFFWNKYMIQDLTEIGTPDVDFWIIPMIQGFVQ IEELVVNYTESSDDEKSSPETPPQESTCVDDIHPRFLVALISRRSRHRAGMRYKRRGVDK NGNVANYVETEQLIHVHNHTLSFVQTRGSVPVFWSQVGYRYNPRPRLDRSEKETVAYFCA HFEEQLNIYKKQVIINLVDQAGREKIIGDAYLKQVLLFNNSHLTYVSFDFHEHCRGMKFE NVQTLTDAIYDIILDMKWCWVDEAGVICKQEGIFRVNCMDCLDRTNVVQAAIARVVMEQQ LKKLGVMPPEQPLPVKCNRIYQIMWANNGDSISRQYAGTAALKGDFTRTGERKLAGVMKD GVNSANRYYLNRFKDAYRQAVIDLMQGIPVTEDLYSIFTKEKEHEALHKENQRSHQELIS QLLQSYMKLLLPDDEKFHGGWALIDCDPSLIDATHRDVDVLLLLSNSAYYVAYYDDEVDK VNQYQRLSLENLEKIEIGPEPTLFGKPKFSCMRLHYRYKEASGYFHTLRAVMRNPEEDGK DTLQCIAEMLQITKQAMGSDLPIIEKKLERKSSKPHEDIIGIRSQNQGSLAQGKNFLMSK FSSLNQKVKQTKSNVNIGNLRKLGNFTKPEMKVNFLKPNLKVNLWKSDSSLETMENTGVM DKVQAESDGDMSSDNDSYHSDEFLTNSKSDEDRQLANSLESVGPIDYVLPSCGIIASAPR LGSRSQSLSSTDSSVHAPSEITVAHGSGLGKGQESPLKKSPSAGDVHILTGFAKPMDIYC HRFVQDAQNKVTHLSETRSVSQQASQERNQMTNQVSNETQSESTEQTPSRPSQLDVSLSA TGPQFLSVEPAHSVASQKTPTSASSMLELETGLHVTPSPSESSSSRAVSPFAKIRSSMVQ VASITQAGLTHGINFAVSKVQKSPPEPEIINQVQQNELKKMFIQCQTRIIQIL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
