Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00864101
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4821nts
Open reading frame: 1 to 4821
ATGTTTCCAAAGAGCAACCTAACAGTCACTTGCTGGGTATGGAGGAGCATGAGGAAGCTC
TTTCTATTGCTTTCTCTCTTGCTGTCCCATGCAGCTCATTTGGAAGGCAAAAAGGATAAT
CAGTTCATCTGGAAACCAGGTCCGTGGGGAAGGTGTACAGGAGACTGTGGTCCCGGAGGA
GTCCAGAGTCGGGCAGTGTGGTGTTTTCATGTTGACGGGTGGACAAGTCACCTGTCTAAC
TGTGGTGAGAGCAACAGGCCTCCAAAGGAAAGAAGTTGTTTCCGAGTTTGTGACTGGCAC
AGTGACCTCTTTCAGTGGGAGGTTTCTGACTGGCACCACTGTGTGCTTGTTCCTTACGCT
CGCGGTGAAGTCAAGCCTCGGACTGCAGAGTGTGTGACGGCTCAGCATGGACTGCAGCAC
CGGATGGTGCGCTGCATTCAGAAGCTGAACCGAACTGTGGTTGCAAATGAAATATGCGAA
CACTTTGCCCTTCAGCCTCCTACAGAACAGGCTTGCCTCATTCCTTGTCCCCGGGATTGT
GTAGTATCTGAGTTCTTACCATGGTCCAACTGTAGCAAGGGATGTGGGAAGAAATTGCAG
CATAGAACTCGCGCGGTCATAGCTCCCCCTCTCTTTGGTGGTTTGCAATGTCCAAATCTG
ACTGAGTCAAGAGCCTGTGATGCTCCCATTTCCTGTCCTCTTGGGGAAGAGGAATATACA
TTTAGCCTTAAGGTTGGACCATGGAGTAAATGCAGACTGCCTCATCTTAAAGAAATTAAT
CCAAGCGGAAGAACTGTTCTGGATTTTAACTCTGATTCAAATGAGCGAGTCACCTTTAAA
CATCAAAGTTACAAAGCACATCATCATTCGAAGTCTTGGGCAATAGAGATAGGTTATCAA
ACCCGGCAGGTTTCGTGTACAAGAAGTGATGGACAAAATGCTATGTTAAGCCTTTGCCTT
CAAGATTCCTTCCCATTGACTGTTCAGTCCTGCATCATGCCCAAAGACTGTGAAACCTCC
CAGTGGTCCTCCTGGAGCCCCTGCTCCAAGACATGCCGTTCAGGGAGTCTCTTGCCAGGA
TTTAGGAGCAGGAGCCGGAACGTGAAGCACATGGCTATTGGAGGTGGAAAGGAGTGTCCT
GAACTTCTTGAGAAAGAGGCCTGCATTGTTGAAGGAGAACTTCTGCAGCAATGTCCCAGG
TATTCCTGGAGAACTTCTGAATGGAAAGAATGCCAAGTCTCTCTCCTCCTCGAGCAGCAG
GATCCCCACTGGCATGTGACGGGACCCGTGTGTGGCGGTGGGATCCAGACCCGGGAGGTG
TACTGTGCCCAGAGCGTACCAGCAGCTGCCGCACTGAGGGCCAAGGAAGTCTCTAGACCT
GTGGAAAAGGCATTATGTGTGGGACCCGCCCCGTTGCCCTCTCAGCTCTGCAATATCCCT
TGCTCTACGGACTGCATAGTATCTTCCTGGTCAGCCTGGGGCCTGTGCATCCATGAAAAC
TGTCATGATCCTCAGGGGAAAAAAGGATTTAGAACGAGGCAGCGCCATGTCCTCATGGAA
TCTACAGGGCCTGCAGGGCATTGCCCTCATTTGGTGGAGTCTGTTCCTTGTGAGGATCCA
ATGTGCTACCGATGGCTGGCATCAGAAGGGATCTGTTTCCCTGATCATGGAAAATGTGGC
CTGGGACATCGTATTCTGAAGGCCGTCTGCCAGAATGACCGCGGAGAAGATGTATCAGGG
AGTCTTTGCCCAGTTCCCCCTCCTCCTGAGAGGAAGTCTTGTGAAATTCCCTGCCGAATG
GACTGTGTGCTGAGCGAGTGGACGGAGTGGTCATCCTGTTCCCAGTCCTGTTCAAATAAA
AACTCAGATGGGAAACAGACCAGGTCAAGAACTATCCTGGCACTGGCTGGGGAAGGTGGA
AAGCCATGTCCCCCTAGTCAGGCTCTCCAAGAGCATCGTTTGTGTAATGACCATTCCTGT
ATGCAGCTTCACTGGGAGACATCGCCTTGGGGCCCTTGTTCTGAGGACACATTGGTAACT
GCCCTTAATGCAACCATTGGCTGGAATGGAGAAGCCACGTGTGGTGTAGGCATTCAGACT
CGGAGAGTCTTCTGTGTCAAGAGTCACGTGGGACAAGTAATGACCAAAAGATGTCCAGAT
TCTACTCGACCTGAAACTGTGCGCCCCTGTTTTCTCCCATGCAAAAAAGACTGTATTGTG
ACTGCTTTCAGTGAGTGGACACCCTGCCCAAGGATGTGCCAAGCAGGAAATGCCACAGTA
AAACAGTCTCGATACAGAATCATCATCCAAGAAGCAGCCAATGGAGGCCAGGAATGCCCA
GATACCTTATATGAGGAGAGAGAGTGTGAAGATGTTTCCTTGTGTCCTGTATATCGGTGG
AAGCCACAGAAATGGAGCCCTTGCATCTTAGTGCCAGAGTCTGTCTGGCAGGGAATAACG
GGCAGCAGTGAAGCCTGTGGAAAGGGGTTACAAACAAGAGCTGTCTCATGCATCTCTGAT
GACAACCGGTCAGCAGAAATGATGGAATGCCTCAAGCAGACAAACGGCATGCCTCTCCTT
GTGCAAGAATGCACAGTCCCATGTCGAGAAGACTGCACCTTCACTGCTTGGTCCAAGTTT
ACGCCCTGCTCCACGAACTGTGAAGCCACAAAAAGTAGGCGGCGACAGCTCACAGGGAAA
AGCAGAAAGAAGGAGAAATGCCAGGATTCTGACCTTTACCCTCTAGTGGAGACAGAACTA
TGTCCTTGTGATGAATTTATATCCCAACCTTATGGAAACTGGTCAGATTGCATTCTTCCA
GAAGGCAGAAGGGAGCCTCACCGAGGACTGCGGGTACAAGCAGACAGCAAAGAATGTGGA
GAAGGCCTGCGCTTTCGAGCAGTAGCCTGTTCTGATAAAAATGGAAGACCTGTTGACCCC
TCCTTCTGCAGCAGCTCTGGTTACATTCAAGAAAAATGTGTCATTCCCTGCCCATTTGAT
TGCAAGTTAAGCGATTGGTCTAGTTGGGGGTCTTGCAGTTCATCTTGTGGAATTGGAGTG
AGAATTCGATCCAAATGGCTAAAAGAAAAACCTTACAATGGAGGACGACCATGTCCCAAA
CTGGATCTCAAGAATCAGGTACATGAGGCAGTCCCATGTTACAGTGAGTGCAATCAGTAT
TCCTGGGTTGTAGAACACTGGTCTTCATGCAAAATCAACAATGAGCTGAGGTCCCTGCGC
TGTGGAGGAGGAACACAATCTAGGAAAATCAGATGTGTGAATACTGCGGATGGTGAAGGT
GGAGCAGTGGATAGCAACCTGTGCAACCAGGATGAAATTCCCCCAGAAACCCAGTCCTGT
TCTCTTATGTGTCCCAATGAGTGTGTCATGTCTGAGTGGGGACTTTGGAGCAAATGCCCA
CAGTCATGCGATCCCCACACAATGCAGAGAAGAACTCGCCACCTGCTAAGACCATCACTG
AACTCAAGGACTTGTGCTGAAGACTCACAGGTGCAGCCTTGCCTCCTGAATGAAAATTGC
TTCCAGTTCCAGTACAATCTAACAGAGTGGAGCACATGCCAGCTGAGTGAAAACGCACCC
TGTGGTCAAGGCGTCAGGACCCGCCTGCTAAGCTGTGTGTGCAGTGATGGCAAGCCAGTC
AGCATGGACCAATGTGAGCAGCATAATTTGGAGAAGCCCCAGAGAATGAGCATTCCCTGC
TTGGTGGAATGCGTGGTCAACTGTCAGCTCTCAGGGTGGACGGCTTGGACAGAGTGTTCA
CAGACCTGTGGCCATGGAGGTCGAATGAGCCGGACTCGATTTATCATTATGCCAACCCAA
GGAGAAGGACGGCCATGCCCCACAGAGCTTACCCAGGAGAAAACCTGCCCAGTGACCCCC
TGCTACAGCTGGGTCCTTGGCAACTGGTCTGCATGTAAATTGGAGGGTGGAGACTGTGGG
GAAGGAGTTCAGATCCGCAGCCTTTCCTGCATGGTCCACAGTGGTTCAATATCTCATGCA
GCTGGACGTGTCGAGGATGCACTGTGTGGAGAAATGCCCTTTCAGGACAGCATCCTGAAG
CAGCTGTGTTCTGTGCCTTGCCCAGGAGACTGCCATTTAACAGAATGGTCAGAGTGGAGC
ACATGTGAATTAACCTGCATTGATGGAAGAAGCTTTGAGACTGTGGGCCGCCAGTCTAGA
TCAAGGACTTTTATAATTCAGTCTTTTGAGAACCAAGACAGCTGCCCCCAACAGGTTCTA
GAAACACGCCCTTGTACAGGAGGCAAATGTTATCACTACACATGGAAAGCAAGTCTTTGG
AACAATAACGAACGAACTGTATGGTGCCAGCGTTCAGATGGCGTTAATGTCACAGGAGGC
TGCTCCCCTCAGGCCCGTCCTGCTGCCATTCGGCAGTGCATTCCAGCCTGCAGAAAACCT
TTCTCCTACTGTACACAGGGTGGAGTCTGTGGTTGTGAGAAGGGCTATACAGAGATAATG
AAATCAAATGGTTTCCTGGATTACTGCATGAAAGTACCAGGCTCAGAGGATAAAAAAGCT
GATGTGAAAAACCTTTCTGGGAAAAACAGACCTGTGAATTCAAAAATACATGATATTTTT
AAAGGATGGTCTCTTCAACCACTTGATCCAGATGGCCGAGTAAAAATTTGGGTTTATGGC
GTTTCAGGTGGCGCTTTTCTCATCATGATTTTCCTAATATTTACTTCCTACCTTGTTTGC
AAGAAGCCAAAACCACATCAAAGCACACCTCCCCAACAGAAGCCTCTGACCTTAGCCTAC
GATGGAGACTTAGACATGTTG
Protein sequence predicted based on nucleotide sequence
MFPKSNLTVTCWVWRSMRKLFLLLSLLLSHAAHLEGKKDNQFIWKPGPWGRCTGDCGPGG VQSRAVWCFHVDGWTSHLSNCGESNRPPKERSCFRVCDWHSDLFQWEVSDWHHCVLVPYA RGEVKPRTAECVTAQHGLQHRMVRCIQKLNRTVVANEICEHFALQPPTEQACLIPCPRDC VVSEFLPWSNCSKGCGKKLQHRTRAVIAPPLFGGLQCPNLTESRACDAPISCPLGEEEYT FSLKVGPWSKCRLPHLKEINPSGRTVLDFNSDSNERVTFKHQSYKAHHHSKSWAIEIGYQ TRQVSCTRSDGQNAMLSLCLQDSFPLTVQSCIMPKDCETSQWSSWSPCSKTCRSGSLLPG FRSRSRNVKHMAIGGGKECPELLEKEACIVEGELLQQCPRYSWRTSEWKECQVSLLLEQQ DPHWHVTGPVCGGGIQTREVYCAQSVPAAAALRAKEVSRPVEKALCVGPAPLPSQLCNIP CSTDCIVSSWSAWGLCIHENCHDPQGKKGFRTRQRHVLMESTGPAGHCPHLVESVPCEDP MCYRWLASEGICFPDHGKCGLGHRILKAVCQNDRGEDVSGSLCPVPPPPERKSCEIPCRM DCVLSEWTEWSSCSQSCSNKNSDGKQTRSRTILALAGEGGKPCPPSQALQEHRLCNDHSC MQLHWETSPWGPCSEDTLVTALNATIGWNGEATCGVGIQTRRVFCVKSHVGQVMTKRCPD STRPETVRPCFLPCKKDCIVTAFSEWTPCPRMCQAGNATVKQSRYRIIIQEAANGGQECP DTLYEERECEDVSLCPVYRWKPQKWSPCILVPESVWQGITGSSEACGKGLQTRAVSCISD DNRSAEMMECLKQTNGMPLLVQECTVPCREDCTFTAWSKFTPCSTNCEATKSRRRQLTGK SRKKEKCQDSDLYPLVETELCPCDEFISQPYGNWSDCILPEGRREPHRGLRVQADSKECG EGLRFRAVACSDKNGRPVDPSFCSSSGYIQEKCVIPCPFDCKLSDWSSWGSCSSSCGIGV RIRSKWLKEKPYNGGRPCPKLDLKNQVHEAVPCYSECNQYSWVVEHWSSCKINNELRSLR CGGGTQSRKIRCVNTADGEGGAVDSNLCNQDEIPPETQSCSLMCPNECVMSEWGLWSKCP QSCDPHTMQRRTRHLLRPSLNSRTCAEDSQVQPCLLNENCFQFQYNLTEWSTCQLSENAP CGQGVRTRLLSCVCSDGKPVSMDQCEQHNLEKPQRMSIPCLVECVVNCQLSGWTAWTECS QTCGHGGRMSRTRFIIMPTQGEGRPCPTELTQEKTCPVTPCYSWVLGNWSACKLEGGDCG EGVQIRSLSCMVHSGSISHAAGRVEDALCGEMPFQDSILKQLCSVPCPGDCHLTEWSEWS TCELTCIDGRSFETVGRQSRSRTFIIQSFENQDSCPQQVLETRPCTGGKCYHYTWKASLW NNNERTVWCQRSDGVNVTGGCSPQARPAAIRQCIPACRKPFSYCTQGGVCGCEKGYTEIM KSNGFLDYCMKVPGSEDKKADVKNLSGKNRPVNSKIHDIFKGWSLQPLDPDGRVKIWVYG VSGGAFLIMIFLIFTSYLVCKKPKPHQSTPPQQKPLTLAYDGDLDML
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
