Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00864261
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 2499nts
Open reading frame: 1 to 2499
ATGGCCATGAGATCCGGGAGGCACCCCTCGCTGCTGCTGCTTCTAGTGCTGCTGCTGTGG
CTGCTGCAGGTCAGTATCATTGACAGTGTTCAACAGGAAACAGATGATCTTACTAAGCAA
ACAAAGGAAAAGATCTACCAGCCTCTACGGCGATCCAAGAGAAGATGGGTTATCACCACC
TTGGAGCTGGAGGAGGAAGACCCGGGACCCTTTCCCAAACTCATTGGTGAGCTGTTCAAT
AATATGTCTTATAACATGTCACTAATGTATCTAATCAGTGGACCTGGTGTGGATGAATAT
CCAGAGATTGGTTTGTTTTCTCTAGAAGATCATGAGAACGGAAGGATATATGTTCACCGC
CCTGTCGATCGAGAAATGACACCATCTTTCACGGTTTATTTTGATGTTGTGGAGCGCTCA
ACAGGAAAAATTGTGGATACATCCTTGATTTTCAACATTAGGATCAGTGATGTGAATGAT
CATGCACCCCAGTTTCCAGAGAAGGAATTTAACATCACTGTGCAAGAAAACCAATCTGCA
GGGCAACCTATTTTTCAGATGTTAGCAGTCGATTTGGATGAAGAAAACACTCCAAATTCT
CAAGTCCTTTACTTCCTCATTTCTCAAACACCATTACTGAAAGAAAGTGGTTTCCGGGTT
GATCGCCTTAGTGGAGAAATACGACTCTCTGGCTGCTTAGATTATGAGACCGCTCCTCAG
TTTACACTGCTAATCAGAGCCAGGGACTGTGGAGAACCGTCACTGTCATCCACGACCACC
GTTCACGTGGATGTGCAAGAAGGCAACAACCACAGGCCTGCATTTACCCAGGAGAACTAT
AAGGTTCAGATTCCTGAAGGCCGAGCCAGCCAGGGCGTGTTGCGTCTCCTGGTTCAAGAT
CGAGATTCTCCATTTACATCAGCTTGGAGAGCAAAATTCAACATATTGCATGGCAATGAA
GAGGGGCATTTTGACATTTCGACTGACCCTGAGACCAACGAAGGGATATTAAATGTTATC
AAGCCTTTGGATTATGAGACTCGCCCAGCGCAAAGCCTCATCATTGTCGTGGAGAATGAG
GAGAGGCTCGTCTTCTGTGAGAGAGGAAAGCTTCAGCCGCCAAGGAAGGCAGCAGCCAGC
GCCACTGTGAGTGTGCAGGTGACAGACGCCAACGACCCACCAGCCTTTCACCCCCAGAGC
TTCATTGTCAATAAAGAGGAGGGCGCCAGGCCTGGGACCCTGTTGGGAACTTTTAATGCC
ATGGATCCAGACAGCCAGATAAGATATGAACTGGTTCATGACCCAGCAAATTGGGTCAGC
GTCGACAAAAACTCCGGAGTGGTCATCACCGTGGAGCCAATTGACCGAGAATCCCCTCAT
GTAAATAACAGTTTTTATGTAATCATCATTCACGCTGTTGATGATGGCTTCCCACCGCAG
ACTGCTACAGGGACCCTAATGCTCTTCCTGTCTGACATCAATGACAACGTCCCGACTCTC
CGGCCACGTTCCCGCTACATGGAGGTCTGTGAGTCTGCTGTGCATGAGCCCCTCCACATC
GAGGCAGAGGATCCGGACCTGGAGCCGTTCTCTGACCCATTTACATTTGAATTGGACAAT
ACCTGGGGAAATGCGGAGGACACATGGAAGTTGGGGAGAAATTGGGGTCAATCAGTTGAA
CTTTTAACCTTGAGAAGCCTGCCACGTGGTAATTACTTGGTGCCACTCTTCATTGGAGAC
AAACAGGGACTTTCCCAGAAGCAAACTGTCCATGTAAGGATCTGCCCCTGTGCCAGTGGG
CTCACATGTGTGGAGCTTGCAGATGCAGAAGTGGGGCTTCATGTGGGGGCCCTGTTCCCT
GTCTGTGCAGCATTTGTGGCTCTGGCAGTGGCTCTGCTTTTTCTGTTGCGATGCTATTTT
GTGCTTGAACCTAAGAGGCATGGATGCTCTGTATCCAATGATGAAGGCCACCAAACACTG
GTCATGTATAATGCGGAGAGCAAAGGCACTTCAGCCCAGACATGGTCAGATGTTGAAGGC
CAGAGGCCGGCTCTGCTCATCTGCACAGCTGCAGCAGGACCCACGCAGGGAGTTAAGGAT
CTCGAGGAAGTGCCTCCATCTGCAGCGAGTCAGTCAGCCCAAGCACGCTGTGCTCTGGGG
AGCTGGGGTTATGGCAAGCCCTTTGAGCCAAGAAGTGTGAAAAACATACACTCTACTCCT
GCTTACCCAGATGCCACAATGCACAGACAACTCCTGGCTCCGGTGGAAGGAAGGATGGCA
GAGACATTGAATCAGAAACTCCATGTTGCCAATGTGCTGGAAGATGACCCCGGCTACCTA
CCTCACGTCTACAGCGAGGAAGGGGAGTGTGGAGGGGCCCCATCCCTCAGCTCTCTGGCC
AGCTTGGAACAGGAGTTGCAACCTGATTTGCTGGACTCTTTGGGTTCAAAAGCGACTCCG
TTTGAGGAAATATATTCAGAGTCAGGTGTTCCTTCCTTG
Protein sequence predicted based on nucleotide sequence
MAMRSGRHPSLLLLLVLLLWLLQVSIIDSVQQETDDLTKQTKEKIYQPLRRSKRRWVITT LELEEEDPGPFPKLIGELFNNMSYNMSLMYLISGPGVDEYPEIGLFSLEDHENGRIYVHR PVDREMTPSFTVYFDVVERSTGKIVDTSLIFNIRISDVNDHAPQFPEKEFNITVQENQSA GQPIFQMLAVDLDEENTPNSQVLYFLISQTPLLKESGFRVDRLSGEIRLSGCLDYETAPQ FTLLIRARDCGEPSLSSTTTVHVDVQEGNNHRPAFTQENYKVQIPEGRASQGVLRLLVQD RDSPFTSAWRAKFNILHGNEEGHFDISTDPETNEGILNVIKPLDYETRPAQSLIIVVENE ERLVFCERGKLQPPRKAAASATVSVQVTDANDPPAFHPQSFIVNKEEGARPGTLLGTFNA MDPDSQIRYELVHDPANWVSVDKNSGVVITVEPIDRESPHVNNSFYVIIIHAVDDGFPPQ TATGTLMLFLSDINDNVPTLRPRSRYMEVCESAVHEPLHIEAEDPDLEPFSDPFTFELDN TWGNAEDTWKLGRNWGQSVELLTLRSLPRGNYLVPLFIGDKQGLSQKQTVHVRICPCASG LTCVELADAEVGLHVGALFPVCAAFVALAVALLFLLRCYFVLEPKRHGCSVSNDEGHQTL VMYNAESKGTSAQTWSDVEGQRPALLICTAAAGPTQGVKDLEEVPPSAASQSAQARCALG SWGYGKPFEPRSVKNIHSTPAYPDATMHRQLLAPVEGRMAETLNQKLHVANVLEDDPGYL PHVYSEEGECGGAPSLSSLASLEQELQPDLLDSLGSKATPFEEIYSESGVPSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
