Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00864295
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4020nts
Open reading frame: 1 to 4020
ATGGCAAAGCGTAGGCCTCACAAACATCCTGGCCGTGAAGAGCTTGTCTTCAAGAGTAGC
TGGTGCAGAAACTCGCCCGTGGCCACCCAGGAGCCCGATGTGCAAGGCAGGTGTGAGTGC
CAGGACGGCATTCCTGGAGATGAAGGCCTGGAGCTGCGGTCTGCGGACTCGGCAGTGCCC
GTGGCCATGACCCAGGCTGCCGTGCGGCCCTGGGCACCCTGCCTGGAGAACATGACCACT
GCCCCAAACGGCCTCGGGCCAGGCCCCGCAGCCCCCTGTGCAGGCTCGGACCTGAAAGAC
GCCAAGATGGTGACCTCCCTTGCGTGTGGAAATGGAGTCTGTGGCTGCAGCCCTGGTGGC
GACACGGACACCCAGGAAGCCAAACTCAGCCCAGCCAAGCTTGTGCGCCTCTTTTCCACC
AGTCGGAAGAGGACGGGTGCCCACCCCGAGCGGCCCCACTCCATGGTCCTGGTGGGGAAC
TCCTCCACATGGAACACCCTCGCCTCCTTCCGGAAAATGGGATCCTTTAAGAAACTGAAG
TCCTCAGTCCTGAAAGGAATTCAGAGCCGAGAGGGGTCAAATGCCTGTTCAAAGGGAGAG
GCTTCGGAGCATGGCCTGGGAAAGTCCATCCCAAATGGCGCTGTCCCAGGAGCCCAGGCA
AGCAGGGGCTCCCCCTTAGCACCGGGACCAGCATGTGGTGCCCTCAGGCCAGCAGAGTGG
GGCACATTGGATGGCTCCGACCTCGAGGACACGGACGATGCCTTCCAGCGGAGCACACAC
CGCTCCCGCAGCCTCCGCAGAGCCTACGGCCTGGGCCGCATCTGCCTGCTGGATGCGCCC
CAGAACCATGCGACACCCACGATAGCCACTGGCCAGGTGCCCGCCGTGTGTGAGATTCTC
GTGAGGGACCCTGAAAACAACAGCATGGGCTACAGGAGGAGCAAGAGCACGGACAATCTT
GCCTTTCTGAAGAAGAGCTCCTTTAAGCGGAAGTCCACCTCCAATCTTGCAGACCTCAGG
ACGGCCCATGACGCACGGGTACCACAGAGGACCCTGAGCAGTTCCTCCACTGACTCCCAA
AAGCTTGGGTCAGGAAGGACCAAACGCTGGAGGAGCCCGATAAGGGCCAAGGACTTTGAC
AGAGTCTTCAAACTTGTGAGCAATGTGACTGAGGCTGCCTGGAGGAGGGAGAGTCCTAGG
AGTGGGGCCCCATCCCCTGGAGAGGCCAGCCTGAGACTTCAGGCACACAGCCGGCTGCAT
GACGACTACTCCCGCCGCGTCTCCAGGAGCACTGAGCAGGACAGCAGGCGGGGCGGGGCG
GTCATGCATGGGACCACTGCAACCTGCACCGTGGCCCCCGGTTTCGGCTCAGCCACCTCT
AAGGGGCCCCACCTAGACGCTGACACTGCCGTATTTCCTCTTGAAACCAAAAGTTCCTGG
GCGGTGGAAAGCGACAGTTCCTGCACTTGCAGCTCTTTGCCAAGCCCGATTGTCCAGGAT
GTGTTGAGCAAAGACTCCTGTGACCCAAACGCTGGCAGCCAGTTGACATTTGACCCTGAG
CAGCCTCCCACCCCTCTAAGGCCCACCACACCCAAGCCCCAGAGCCCTCAGAGCCCCCAG
AGCCCCGGGGCAGGAAGTGCCAGCTGTCACAGCAATCACAGTGCCCTGTCCGCGAATTCA
GAGGAAAGTGAAGGAAGGGCAGAAGAGCCTGCTCAGAGAGAGCCAGGGCCTGTGTCCTTG
CAGGATCCCCTGGAAGCCACACATGGTGATGAGGGCAGCAAGGACCTTCTGGTGAACATT
GGTGTGGCAGCCGGCCCAGAAGAAAAGGAGAAGGAGGAGGTCGTCCCTGATGGCCCCTGG
AGGCGAAGCTCATCACAGGATGAGGAAAGGACAGAGGCACAGAGAACCCCCAAGAGGAGA
TGGGGCTCTGGGAGACGGCCAAGGCCTCGGCCATTCTCTGACTACGGCCAGCTGGCCAGC
CGCAGTTTGTCTATTCCTGAAGACTCGGTTGCTGCAGACCCCCAGAAGGAAGACCGTGTG
GACGAGGACCCCCAGGCAAGCATGACTTCTGCCAGCCCTGAAGACCAGAATGCTCCAGTG
GGCTGCCCCAAAGGAGCCCGGAGAAGGCGCCCCATTTCCGTGATAGGTGGGGTCAGCTTG
TATGGGACCAACCAGACGGAGGAACTGGACAATCTTCTGACCCAACCGGCTTCCAGGCCG
CCCATGCCTGCTCACCAGGTGCCACCCTACAAGGCTGTGTCGGCCCGGTTCCGGCCCTTC
ACATTCTCCCAGAGCACCCCCATTGGGTTGGACCGTGTGGGACGCCGGCGGCAGATGAGA
GCATCCAACGTTTCTTCAGATGGAGGTACTGAGCCCTCTGCCTTAGTGGATGACAACGGT
AGTGAGGAGGACTTCAGCTATGAAGACCTCTGCCAGGCCAGCCCTCGGTACCTGCAGCCC
GGCGGGGAGCAGCTGGCCATCAATGAGCTGATCAGTGATGGCAACGTGGTCTGCGCAGAA
GCCCTGTGGGACCATGTGACCATGGATGACCAGGAACTGGGCTTCAAAGCCGGGGATGTC
ATCCAGGTTCTGGAAGCCTCCAACAAGGACTGGTGGTGGGGCCGCAGTGAAGATAAGGAA
GCCTGGTTCCCCGCGAGCTTCGTCAGATTGCGAGTGAATCAGGAAGAGCTGTCGGAAAAC
TCCAGCAGCACCCCCAGTGAGGAGCAGGACGAGGAGGCCAGCCAGAGCCGCCACAGACAC
TGTGAGAACAAGCAGCAGATGCGGACCAACGTCATCCGGGAGATCATGGACACCGAGCGG
GTGTACATCAAACACCTCAGGGACATCTGTGAGGGCTATATCCGACAGTGCCGCAAGCAC
ACAGGAATGTTCACCGTTGCGCAGCTAGCCACTATTTTTGGAAACATTGAAGATATTTAC
AAATTCCAAAGAAAGTTTCTGAAAGACCTTGAGAAACAGTACAACAAAGAGGAACCTCAC
TTAAGTGAAATAGGATCTTGCTTTCTTCAAAATCAAGAGGGCTTTGCCATCTATTCCGAG
TACTGCAACAACCACCCGGGCGCCTGCCTGGAGCTCGCCAACCTCATGAAGCAGGGCAAG
TACAGACATTTCTTTGAAGCCTGCCGCCTGCTGCAGCAGATGATTGACATCGCCATCGAC
GGGTTCCTGCTCACACCAGTGCAGAAGATCTGCAAATACCCGCTGCAGCTGGCCGAGCTG
CTCAAGTATACCACACAGGAACACGGTGATTACAGCAACATAAAGGCAGCATATGAGGCC
ATGAAGAATGTGGCCTGTCTGATCAACGAGCGCAAGCGCAAGCTGGAGAGCATCGACAAG
ATAGCTCGCTGGCAGGTGTCTATCGTGGGCTGGGAGGGACTGGATATCTTAGACCGAAGC
TCAGAATTGATTCATTCTGGGGAGCTGACCAAAATCACTAAGCAAGGCAAAAGCCAGCAG
CGGACGTTCTTCCTGTTTGACCACCAGCTGGTGTCCTGCAAGAAGGACCTGCTGCGCAGG
GACATGCTGTACTACAAGGGCCGGCTGGACATGGATGAGATGGAGCTTGTGGACCTGGGG
GATGGGCGCGACAAGGACTGCAACCTCAGCGTGAAAAATGCCTTCAAGCTCGTCAGTAGG
ACCACAGACGAGGTTTATTTGTTTTGTGCCAAAAAACAAGAAGACAAGGCGAGGTGGCTG
CAGGCCTGTGCAGATGAAAGGAGGCGGGTGCAAGAGGACAAGGAGATGGGAATGGAAATT
TCAGAAAACCAGAAGAAACTTGCCATGTTAAATGCTCAAAAGGCAGGACATGGAAAGTCA
AAAGGCTACAACAGGTGCCCTGTGGCCCCACCGCACCAGGGCCTGCACCCCATCCACCAG
CGCCACATCACTATGCCCACAAGCGTCCCCCAGCAGCAGGTCTTTGGCCTGGCGGAACCC
AAGAGGAAGTCCTCGCTCTTCTGGCACACCTTCAACAGGCTCACCCCCTTCCGGAAATTG
Protein sequence predicted based on nucleotide sequence
MAKRRPHKHPGREELVFKSSWCRNSPVATQEPDVQGRCECQDGIPGDEGLELRSADSAVP VAMTQAAVRPWAPCLENMTTAPNGLGPGPAAPCAGSDLKDAKMVTSLACGNGVCGCSPGG DTDTQEAKLSPAKLVRLFSTSRKRTGAHPERPHSMVLVGNSSTWNTLASFRKMGSFKKLK SSVLKGIQSREGSNACSKGEASEHGLGKSIPNGAVPGAQASRGSPLAPGPACGALRPAEW GTLDGSDLEDTDDAFQRSTHRSRSLRRAYGLGRICLLDAPQNHATPTIATGQVPAVCEIL VRDPENNSMGYRRSKSTDNLAFLKKSSFKRKSTSNLADLRTAHDARVPQRTLSSSSTDSQ KLGSGRTKRWRSPIRAKDFDRVFKLVSNVTEAAWRRESPRSGAPSPGEASLRLQAHSRLH DDYSRRVSRSTEQDSRRGGAVMHGTTATCTVAPGFGSATSKGPHLDADTAVFPLETKSSW AVESDSSCTCSSLPSPIVQDVLSKDSCDPNAGSQLTFDPEQPPTPLRPTTPKPQSPQSPQ SPGAGSASCHSNHSALSANSEESEGRAEEPAQREPGPVSLQDPLEATHGDEGSKDLLVNI GVAAGPEEKEKEEVVPDGPWRRSSSQDEERTEAQRTPKRRWGSGRRPRPRPFSDYGQLAS RSLSIPEDSVAADPQKEDRVDEDPQASMTSASPEDQNAPVGCPKGARRRRPISVIGGVSL YGTNQTEELDNLLTQPASRPPMPAHQVPPYKAVSARFRPFTFSQSTPIGLDRVGRRRQMR ASNVSSDGGTEPSALVDDNGSEEDFSYEDLCQASPRYLQPGGEQLAINELISDGNVVCAE ALWDHVTMDDQELGFKAGDVIQVLEASNKDWWWGRSEDKEAWFPASFVRLRVNQEELSEN SSSTPSEEQDEEASQSRHRHCENKQQMRTNVIREIMDTERVYIKHLRDICEGYIRQCRKH TGMFTVAQLATIFGNIEDIYKFQRKFLKDLEKQYNKEEPHLSEIGSCFLQNQEGFAIYSE YCNNHPGACLELANLMKQGKYRHFFEACRLLQQMIDIAIDGFLLTPVQKICKYPLQLAEL LKYTTQEHGDYSNIKAAYEAMKNVACLINERKRKLESIDKIARWQVSIVGWEGLDILDRS SELIHSGELTKITKQGKSQQRTFFLFDHQLVSCKKDLLRRDMLYYKGRLDMDEMELVDLG DGRDKDCNLSVKNAFKLVSRTTDEVYLFCAKKQEDKARWLQACADERRRVQEDKEMGMEI SENQKKLAMLNAQKAGHGKSKGYNRCPVAPPHQGLHPIHQRHITMPTSVPQQQVFGLAEP KRKSSLFWHTFNRLTPFRKL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
