Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00865116
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4734nts
Open reading frame: 1 to 4734
ATGGAGGCTGGCTCAGTGGTTCGAGCCATTTTTGACTTCTGCCCTAGCGTATCAGAAGAA
CTGCCGCTCTTTGTGGGAGATATTATTGAGGTGCTGGCAGTGGTGGATGAATTTTGGCTT
CTAGGAAAGAAGGAGGATGTTACAGGACAATTCCCCAGCAGTTTTGTGGAAATTGTGACC
ATTCCCAGTCTAAAAGAGGGAGAGAGGCTGTTTGTGTGCATTTGTGAATTCACATCCCAA
GAGTTGGATAATCTTCCCCTCCATCGAGGTGACCTGGTGATTCTCGATGGCATTCCCACT
GCAGGCTGGCTGCAGGGCCGAAGCTGCTGGGGCGCACGGGGCTTCTTCCCATCTTCATGT
GTCCGCGAGCTCTGCCTCTCCTCACAGAGCCGGCAGTGGCACTCCCAGAGCGCCCTGTTT
CAGATTCCGGAATATTCCATGGGACAAGCCCGGGCCCTAATGGGGCTTTCTGCTCAGCTG
GATGAAGAGCTGGACTTCAGGGAAGGGGATGTGATCACCATTATTGGAGTTCCTGAACCA
GGCTGGTTTGAAGGGGAGTTAGAGGGCCGAAGAGGCATTTTTCCAGAAGGTTTTGTAGAG
CTGTTGGGGCCCCTGAGGACTGTGGATGAGTCAGTAAGTTCTGGAAATCAAGATGACTGC
ATTGTTAATGGTGAAGTAGATACCCCTGTAGGAGAAGAAGAGATAGGGCCGGATGAGGAT
GAGGAGGAGCCAGGGACCTATGGGGTCGCCCTGTACAGATTCCAAGCCCTGGAGCCAAAT
GAGCTGGATTTCGAGGTCGGGGATAAAATCCGAATTCTGGCGACCTTGGAAGATGGCTGG
CTGGAAGGATCCCTGAAGGGCAGGACAGGCATCTTTCCTTACCGGTTTGTGAAATTATGT
CCTGACACACGGGTGGAGGAAACCATGGCTCTGCCCCAGGAAGGCAGCCTTGCCAGGATC
CCGGAAACTTCTTTGGATTGTTTGGAGAACACCTTAGGAGTAGAGGAACAAAGACATGAA
ACCAGTGACCATGAGGCCGAGGAGCCTGACTGCATTATTTCTGAAGCACCCACTTCTCCC
CTCGGTCATCTGACTTCAGAGTATGACACAGACAGAAACTCTTATCAGGACGAGGACACC
GCAGGAGGGCCCCCGAGAAGCCCAGGCGTGGAGTGGGAAATGCCTCTTGCCACAGACTCT
CCCACATCTGACCCTACAGAAGTAGTCAATGGTATTTCCTCCCAACCTCAGGTCCCTTTT
CATCCCAACTTGCAGAAAAGCCAGTATTATTCTACAGTGGGAGGGAGCCACCCGCACTCA
GAACAGTACCCCGACCTTCTTCCCCTAGAAGCAAGGACTAGAGACTATGCCAGCCTACCT
CCCAAAAGAATGTATTCCCAGCTAAAAACTCTTCAGAAGCCAGTGCTCCCTCTTTACAGG
GGCTCTTCTGTTTCAGCTTCAAGGGTAGTCAAACCCAGACAATCAAGTCCTCAGCTCCAC
AACCTAGCAAGTTATACTAAAAAGCACCACACGTCCAGTGTTTACTCCATCTCAGAGAGA
TTGGAGATGAAGCCTGGTCCGCAAGCCCAAGGGCTTGTTATGGAAGCAGCAACACATTCA
CAGGGAGACGGCAGCACTGACCTGGACTCGAAGCTGACACAACAGCTGATCGAGTTTGAG
AAGAGCTTGGCAGGGCCCGGCACAGAGCCAGATAAAATTTTACGCCACTTTTCAATCATG
GACTTTAACTCTGAGAAGGATATTGTCCGAGGTTCCTCAAAGTTAATCACCGAGCAGGAG
CTGCCGGAAAGGAGAAAGGCCCTAAGGCCACCGCCACCTCGTCCCTGTACTCCGGTATCC
ACTTCTCCCCATTTGCTGGTTGACCAGAACCTAAAACCTGCACCACCCTTGGTGGTGCGA
CCCTCTCGCCCAGCTCCCCTGCCTCCCTCAGCACAGCAGAGAACGAATGCGGTATCCCCC
AAGCTCCTATCTCGACACCGTCCTACCTGTGAGACCTTAGAAAAGGAGGGCCCTGGTCAT
ATGGGAAGGAGTCTGGACCAGACCTCCCCATGCCCCTTAGTGCTGGTGAGGATTGAGGAA
ATGGAGCGGGACTTGGATATGTACAGTAGAGCTCAAGAAGAGCTAAACCTCATGCTGGAG
GAGAAGCAGGATGAATCATCAAGAGCAGAGACCCTCGAGGATCTCAAGTTCTGTGAAAGT
AACATTGAAAGTTTGAATATGGAACTCCAGCAACTAAGAGAAATGACGCTCCTCTCCTCC
CAGTCTTCATCACTGGTGGCCCCTTCTGGGTCTGTGTCTGCCGAAAATCCAGAGCAGAGG
ATGCTGGAGAAGAGAGCCAAGGTCATAGAAGAACTTCTTCAGACAGAAAGAGACTACATT
CGGGATCTGGAAATGTGTATTGAGCGGATCATGGTACCCATGCAGCAGGCACAGGTACCA
AACATTGATTTTGAGGGACTTTTTGGAAATATGCAGATGGTGATTAAGGTCTCGAAGCAA
TTATTGGCTGCTCTGGAAATCAGCGATGCTGTAGGACCTGTGTTTCTTGGTCACCGGGAT
GAGCTTGAGGGAACATACAAGATTTACTGCCAGAATCATGATGAGGCCATTGCGCTGCTT
GAAATCTACGAGAAGGATGAGAAGATCCAGAAGCATCTTCAGGACTCCTTGGCAGATCTG
AAGAGCCTATACAACGAATGGGGATGCACAAATTATATTAACCTGGGCTCCTTCCTCATC
AAACCAGTACAGAGAGTAATGCGTTACCCGCTGTTGCTAATGGAGTTGCTGAATTCCACC
CCAGAATCCCACCCAGATAAAGTGCCTTTAACCAATGCAGTCCTTGCGGTCAAGGAAATC
AACGTTAACATTAATGAATATAAACGGCGAAAGGACCTGGTCCTCAAGTACCGTAAGGGT
GATGAAGATAGCCTTATGGAGAAAATTTCCAAACTGAACATCCACTCCATCATCAAGAAA
TCCAACCGAGTTAGCAGTCACCTGAAGCATCTCACTGGCTTTGCTCCTCAGATAAAAGAT
GAAGTATTTGAAGAAACAGAAAAAAACTTCCGAATGCAAGAAAGATTGATTAAGTCTTTT
ATCCGAGACCTGTCTCTCTACCTCCAGCACATCCGGGAGTCCGCATGTGTGAAAGTGGTG
GCTGCTGTGAGCATGTGGGATGTGTGCATGGAGAGAGGACACCGGGACCTGGAGCAGTTT
GAGAGGGTGCATCGCTACATCAGTGACCAGCTCTTCACAAACTTTAAGGAGAGGACAGAG
CGGCTTGTCATCTCCCCCTTAAATCAGTTACTGAGCATGTTTACAGGGCCCCATAAGCTG
GTACAGAAACGCTTTGACAAGCTCCTGGACTTCTATAACTGTACAGAACGGGCAGAAAAG
CTAAAGGACAAGAAGACCCTGGAGGAGCTGCAGTCGGCCCGGAACAACTATGAGGCCCTG
AATGCACAGCTGCTGGATGAGCTGCCCAAGTTCCACCAGTACGCCCAGGGCCTCTTCACC
AACTGTGTCCACGGCTATGCTGAAGCCCACTGTGACTTTGTGCACCAGGCTCTGGAGCAA
TTAAAGCCACTGCTTTCGTTACTCAAAGTGGCTGGCAGAGAGGGAAACCTTATTGCCATC
TTCCACGAAGAGCACAGCAGAGTTCTGCAGCAACTCCAGGTTTTTACCTTCTTCCCGGAG
TCTCTTCCAGCTACCAAGAAGCCATTTGAGAGGAAAACCATTGACCGCCAGTCTGCTCGA
AAGCCACTCCTGGGCCTGCCAAGTTACATGCTACAGTCAGAAGAACTCCGGGCCTCCCTC
CTGGCCAGGTATCCCCCTGAAAAACTCTTCCAGGCAGAACGGAACTTCAATGCTGCTCAA
GACTTGGATGTCTCACTTTTGGAAGGTGACCTGGTGGGTGTGATTAAGAAAAAAGACCCC
ATGGGCAGCCAGAACCGCTGGCTGATTGACAATGGAGTCACCAAAGGCTTCGTGTACAGC
TCTTTCCTAAAGCCCTACAATCCTCGCCGCAGCCACTCCGATGCCTCCGTGGGTAGCCAC
TCCTCCACAGAGTCTGAGCACGGCAGCTCCTCCCCCAGGTTCCCACGCCAGAACAGCGGC
AGCACCCTGACCTTCAACCCCAGCAGCATGGCTGTATCCTTTACCTCGGGGTCTTGCCAG
AAGCAGCCTCAAGATGCATCTCCTCCGCCAAAAGAATGTGACCAAGGAACTCTCAGTGCA
TCCCTAAATCCGAGTAATTCAGAGAGTAGTCCTTCCAGATGCCCTTCAGACCCAGACTCC
ACCTCCCAGCCAAGGTCAGGGGACTCTGCAGATGTAGCTAGAGATGTAAAGCAACCCACT
GCCACGCCGAGGAGCTACCGGAACTTCAGGCATCCAGAAATAGTTGGCTACTCCGTACCA
GGACGAAATGGGCAAAGTCAAGACCTCGTCAAAGGATGTGCAAGAACAGCCCAGGCTCCG
GAAGACAGAAGTACAGAGCCAGATGGCAGTGAGGCAGAAGGCAACCAGGTCTATTTTGCT
GTCTACACCTTCAAGGCACGAAACCCAAATGAGCTGAGCGTGTCAGCCAATCAGAAACTC
AAGATCCTCGAGTTTAAAGATGTTACAGGAAATACAGAGTGGTGGTTAGCTGAGGTTAAC
GGGAAGAAGGGCTACGTTCCCTCCAATTATATCCGCAAAACCGAGTACACCTTG
Protein sequence predicted based on nucleotide sequence
MEAGSVVRAIFDFCPSVSEELPLFVGDIIEVLAVVDEFWLLGKKEDVTGQFPSSFVEIVT IPSLKEGERLFVCICEFTSQELDNLPLHRGDLVILDGIPTAGWLQGRSCWGARGFFPSSC VRELCLSSQSRQWHSQSALFQIPEYSMGQARALMGLSAQLDEELDFREGDVITIIGVPEP GWFEGELEGRRGIFPEGFVELLGPLRTVDESVSSGNQDDCIVNGEVDTPVGEEEIGPDED EEEPGTYGVALYRFQALEPNELDFEVGDKIRILATLEDGWLEGSLKGRTGIFPYRFVKLC PDTRVEETMALPQEGSLARIPETSLDCLENTLGVEEQRHETSDHEAEEPDCIISEAPTSP LGHLTSEYDTDRNSYQDEDTAGGPPRSPGVEWEMPLATDSPTSDPTEVVNGISSQPQVPF HPNLQKSQYYSTVGGSHPHSEQYPDLLPLEARTRDYASLPPKRMYSQLKTLQKPVLPLYR GSSVSASRVVKPRQSSPQLHNLASYTKKHHTSSVYSISERLEMKPGPQAQGLVMEAATHS QGDGSTDLDSKLTQQLIEFEKSLAGPGTEPDKILRHFSIMDFNSEKDIVRGSSKLITEQE LPERRKALRPPPPRPCTPVSTSPHLLVDQNLKPAPPLVVRPSRPAPLPPSAQQRTNAVSP KLLSRHRPTCETLEKEGPGHMGRSLDQTSPCPLVLVRIEEMERDLDMYSRAQEELNLMLE EKQDESSRAETLEDLKFCESNIESLNMELQQLREMTLLSSQSSSLVAPSGSVSAENPEQR MLEKRAKVIEELLQTERDYIRDLEMCIERIMVPMQQAQVPNIDFEGLFGNMQMVIKVSKQ LLAALEISDAVGPVFLGHRDELEGTYKIYCQNHDEAIALLEIYEKDEKIQKHLQDSLADL KSLYNEWGCTNYINLGSFLIKPVQRVMRYPLLLMELLNSTPESHPDKVPLTNAVLAVKEI NVNINEYKRRKDLVLKYRKGDEDSLMEKISKLNIHSIIKKSNRVSSHLKHLTGFAPQIKD EVFEETEKNFRMQERLIKSFIRDLSLYLQHIRESACVKVVAAVSMWDVCMERGHRDLEQF ERVHRYISDQLFTNFKERTERLVISPLNQLLSMFTGPHKLVQKRFDKLLDFYNCTERAEK LKDKKTLEELQSARNNYEALNAQLLDELPKFHQYAQGLFTNCVHGYAEAHCDFVHQALEQ LKPLLSLLKVAGREGNLIAIFHEEHSRVLQQLQVFTFFPESLPATKKPFERKTIDRQSAR KPLLGLPSYMLQSEELRASLLARYPPEKLFQAERNFNAAQDLDVSLLEGDLVGVIKKKDP MGSQNRWLIDNGVTKGFVYSSFLKPYNPRRSHSDASVGSHSSTESEHGSSSPRFPRQNSG STLTFNPSSMAVSFTSGSCQKQPQDASPPPKECDQGTLSASLNPSNSESSPSRCPSDPDS TSQPRSGDSADVARDVKQPTATPRSYRNFRHPEIVGYSVPGRNGQSQDLVKGCARTAQAP EDRSTEPDGSEAEGNQVYFAVYTFKARNPNELSVSANQKLKILEFKDVTGNTEWWLAEVN GKKGYVPSNYIRKTEYTL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
