Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00865363
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5583nts
Open reading frame: 1 to 5583
ATGGGAGCGGGATCGGCGCGGGGGGCCCGAGGCACAGCGGCGGCGGCGGCGGCGCGCGGG
GGGGGGTTTCTCTTCTCCTGGATCTTAGTCTCGTTTGCCTGTCACCTGGCCTCCACCCAA
GGAGCTCCTGAAGATGTGGACATCCTCCAGCGGCTGGGCCTCAGCTGGACGAAGGCCGGG
AGCCCTGCACCCCCGGGAGTCATTCCTTTCCAGTCGGGCTTCATCTTTACGCAGCGGGCC
CGGCTCCAGGCTCCCACGGGCACCGTCATTCCTGCCGCCTTGGGCACAGAGCTGGCACTG
GTGCTGAGCCTCTGCTCCCACCGGGTGAACCATGCCTTCCTCTTCGCTGTCCGCAGCCAG
AAACGCAAGCTGCAGCTGGGCCTGCAGTTCCTCCCCGGCAAGACGGTCGTCCACCTCGGG
TCCCGGCGCTCAGTGGCCTTCGACCTCGACATGCACGACGGGCGCTGGCACCACCTGGCC
CTCGAGCTCCGAGGCCGCACAGTCACTCTGGTGACTGCCTGCGGGCAGCGCCGGGTGCCT
GTCCTGCTGCCTTTCCACAGGGACCCTGCACTCGACCCTGGGGGCTCCTTCCTCTTTGGG
AAGATGAACCCGCATGCAGTCCAGTTTGAAGGTGCTCTCTGCCAGTTCAGTATCTACCCT
GTGACGCAGGTCGCTCACAATTACTGTACCCACCTGAGGAAGCAGTGTGGACAGGCTGAC
ACGTACCAGTCCCCACTGGGACCTCTCTTCTCCCAAGACTCTGGCAGACCTTTTACCTTC
CAGTCCGACCTCGCCCTGCTAGGCCTGGAGAACTTGACCACTGCCACACCAGCCCTGGGG
TCACTGCCAGCAGGCAGGGGACCCAGGGGGACTGTGGCACCCGCCACGCCCACCAAGCCC
CAAAGGACTAGCCCCACAAACCCTCACCAGCATATGGCGGTGGGAGGCCCAGCCCAAACC
CCGCTGCTACCTGCCAAGCTGTCAGCCAGTAACGCACTTGATCCCATGCTCCCAGCCTCT
GTTGGCGGCTCTACCAGAACGCCTCGCCCTGCGGCCGCTCAACCATCACAGAAGATCACA
GCCACCAAAATCCCCAAAAGCCTCCCTACCAAGCCTTCGGCCCCTTCTACTTCAATTGTG
CCCATCAAAAGCCCCCATCCTACCCAGAAAACAGCTCCATCTTCATTTACAAAGTCAGCC
CTACCCACTCAGAAGCAAGTGCCACCTACTTCCCGTCCAGTTCCTGCCAGAGTCTCCCGT
CCCGCAGAGAAGCCCATCCAGAGGAACCCGGGAATGCCCAGGCCCCCACCGCCCAGCACC
CGGCCCCTACCTCCTACCACCAGCTCCTCTAAAAAACCCATTCCCACACTAGCTCGGACT
GAGGCCAAGATAACCAGCCATGCCAGTAAGCCGGCCTCTGCCCGCACCAGCACCCACAAA
CCTCCCCCATTTACTGCTTTATCCTCATCTCCTGCCCCTACTCCTGGTTCTACCAGGAGT
ACTCGGCCACCAGCCACGATGGTACCTCCAACTTCGGGCACCAGCACTCCCAGAACAGCA
CCTGCCGTCCCCACTCCTGGCTCAGCTCCCACTGGAAGCAAGAAGCCCATTGGATCGGAA
GCCTCAAAGAAAGCCGGACCCAAGAGCAGCCCCCGGAAGCCTGTCCCCCTCAGACCTGGG
AAGGCAGCCAGGGATGTCCCCTTGAGCGATCTGACAACCAGGCCTAGCCCCAGACAGCCC
CAGCCCAGTCAGCAGACCACCCCGGCCCTGGTATTGGCCCCGGCGCAATTCCTGTCCTCC
AGCCCCCGGCCCACGAGCAGTGGCTATTCGATCTTCCACCTGGCAGGATCTACGCCTTTC
CCTCTGCTGATGGGGCCTCCGGGACCCAAGGGAGACTGTGGCTTGCCGGGTCCCCCTGGG
CTACCTGGGCTACCTGGAATCCCTGGTGCACGTGGGCCTCGGGGTCCTCCTGGGCCTTAT
GGAAATCCAGGTCTCCCCGGCCCTCCTGGAGCCAAAGGACAGAAAGGGGACCCAGGGCTC
TCACCAGGAAAGGCCCACGATGGGGCAAAGGGTGACATGGGCTTGCCTGGGCTCTCCGGG
AATCCAGGACCTCCGGGACGAAAGGGACACAAGGGCTATCCTGGACCGGCAGGGCACCCC
GGAGAACAGGGGCAGCCAGGACCTGAGGGCAGCCCAGGGGCCAAAGGTTACCCTGGCAGG
CAGGGGTTACCTGGACCGGTAGGAGATCCCGGCCCCAAAGGCAGCAGGGGCTACATTGGG
CTCCCAGGGCTCTTCGGCCTGCCAGGGTCTGATGGAGAACGAGGCCTGCCTGGCGTTCCT
GGCAAGAGGGGCAAGATGGGTATGCCGGGGTTTCCTGGAGTCTTTGGGGAAAGAGGCCCT
CCTGGACTGGATGGAAATCCTGGAGAACTGGGCCTGCCAGGCCCCCCTGGAGTCCCCGGC
CTCATTGGTGACTTAGGAGTGTTGGGTCCGATTGGCTACCCGGGACCCAAGGGCATGAAG
GGACTGATGGGCAGCGTGGGGGAGCCCGGACTGAAAGGTGATAAGGGTGAACAAGGGGTT
CCAGGTGTGTCAGGAGATCCCGGATTCCAAGGAGACAAGGGGAGCCAGGGGTTGCCAGGG
TTCCCCGGTGCACGGGGGAAGCCAGGGCCTCTGGGCAAAGTCGGAGACAAAGGATCCATT
GGGTTTCCCGGGCCCCCTGGACCCGAGGGATTCCCAGGAGACATCGGCCCCCCTGGCGAC
AATGGCCCAGAAGGCATGAAGGGTAAGCCTGGAGCCCGAGGCCTGCCGGGACCCCGTGGG
CAGCTGGGGCCCGAGGGAGATGAGGGACCCATGGGGCCGCCAGGGGCCCCTGGCTTGGAG
GGTCAGCCTGGCAGGAAGGGGTTTCCTGGGAGGCCCGGCCTGGATGGCGTGAAGGGGGAA
CCAGGGGATCCTGGTCGGCCGGGGCCTGTGGGAGAGCAGGGATTTATGGGATTCATTGGT
CTGGTCGGGGAGCCAGGAATCGTGGGAGAAAAGGGTGATCGTGGCATGATGGGACCCCCA
GGCGTGCCTGGACCCAAGGGGTCGATGGGTCATCCTGGAATGCCAGGTGGTATGGGGACC
CCTGGAGAGCCTGGACCCCAGGGTCCTCCAGGATCTCGAGGCCCACCAGGCATGAGGGGA
GCAAAGGGACGTCGGGGCCCCCGAGGACCGGACGGACCAGCTGGGGAGCAAGGGTCCAGG
GGCCTGAAGGGCCCTCCAGGACCCCAGGGCAGACCGGGCCGGCCTGGACAGCAGGGTGTG
GCTGGTGAGCGAGGCCACTTGGGCTCGAGAGGCTTTCCTGGCATCCCGGGTCCCTCAGGC
CCCCCAGGCACCAAGGGCCTCCCAGGAGAACCGGGCCCTCAGGGACCCCAGGGGCCAATT
GGGCCTCCAGGAGAGATGGGACCCAAGGGGCCGCCTGGTGCAGTGGGAGAACCGGGCCTT
CCTGGGGAAGCCGGGATGAAGGGTGACCTTGGACCCCTGGGCACTCCTGGGGAGCAGGGC
CTCATTGGGCAACGGGGAGAGCCAGGCCTTGAGGGTGACAGTGGCCCCATGGGACCTGAT
GGGCTGAAGGGGGACAGGGGAGACCCAGGGCCTGATGGAGAACATGGCGAGAAAGGCCAG
GAAGGGCTGATGGGTGAGGACGGGCCCCCCGGCCCCCCTGGCGTCACTGGTGTCCGGGGT
CCTGAAGGAAAATCAGGGAAGCAAGGCGAGAAGGGCCGCACTGGAGCCAAGGGTGCCAAG
GGCTATCAAGGACAGCTGGGTGAGATGGGCGTCCCTGGAGACCCTGGACCCCCTGGCACT
CCAGGCCCTAAAGGGTCCCGGGGCAGCCTGGGACCAACGGGTGCTCCGGGACGCATGGGG
GCCCAAGGAGAACCGGGACTGGCTGGTTATGATGGACACAAAGGCATTGTGGGACCCCTT
GGACCTCCTGGACCAAAAGGCGAAAAGGGGGAGCAGGGCGAGGACGGCAAGGCTGAGGGG
CCCCCTGGGCCACCTGGAGATCGGGGCCCTGTGGGTGATCGAGGAGACCGCGGGGAACCG
GGAGACCCTGGGTACCCTGGACAGGAGGGTGTGCAAGGCCTCCGTGGAAAGCCAGGCCAG
CAGGGCCAACCCGGGCATCCGGGACCCCGGGGGTGGCCGGGACCCAAAGGATCGAAAGGC
GCAGAGGGACCAAAGGGAAAGCAAGGCAAGGCAGGGGCCCCAGGCCGGAGGGGGGTCCAG
GGCCTGCAGGGGCTGCCAGGGCCCCGGGGCGTGGTGGGGAGACAGGGCCTCGAGGGCATC
GCTGGACCAGATGGGCTTCCTGGCAGGGACGGGCAAGCAGGACAGCAGGGGGAGCAGGGA
GACGATGGGGACCCTGGCCCCATGGGCCCTGCTGGGAAGAGAGGAAATCCAGGTGTGGCC
GGCTTACCTGGAGCACAGGGACCCCCAGGATTCAAGGGTGAGAGTGGGTTACCCGGACAG
CTGGGTCCCCCTGGCAAGCGAGGAACAGAGGGCAGAACGGGGCTCCCTGGAAACCAGGGG
GAGCCTGGGTCCAAAGGCCAGCCGGGCGACTCTGGCGAGATGGGCTTCCCAGGAATGGCA
GGTCTCTTCGGACCCAAGGGCCCGCCTGGAGACATTGGCTTCAAAGGCATCCAGGGCCCT
CGGGGGCCACCTGGCTTGATGGGAAAGGAAGGCATCGTCGGGCCCCTCGGAATCCTGGGA
CCTTCGGGACTCCCGGGTCCGAAGGGTGACAAAGGCAGCCGTGGGGACTGGGGATTGCAA
GGTCCGAGGGGTCCTCCCGGCCCCAGAGGGCGGCCCGGCCCCCCGGGTCCTCCAGGGGGT
CCTATCCAATTGCAACAAGATGATCTTGGGGCAGCTTTCCAGACGTGGATGGACACCAGT
GGAGCACTCAGGCCAGAGAGTTACAGCTATCCAGACCGGCTGGTGCTGGACCAGGGAGGA
GAGATCTTTAAAACCTTACACTACCTCAGCAACCTCATCCAGAGCATTAAGACGCCCCTG
GGCACCAAAGAGAACCCCGCCCGGGTCTGCAGGGACCTCATGGACTGTGAGCAGAAGATG
GTGGATGGTACCTACTGGGTGGATCCAAACCTTGGCTGCTCCTCTGACACCATCGAGGTC
TCCTGCAACTTCACTCATGGTGGACAGACGTGTCTCAAGCCCATCACGGCCTCCAAGGTC
GAGTTTGCCATCAGCCGGGTCCAGATGAATTTCCTGCACCTGCTAAGCTCCGAGGTGACC
CAGCACATCACCATCCACTGCCTTAACATGACCGTGTGGCAGGAGGGCACTGGGCAGACC
CCAGCCAAGCAGGCCGTACGCTTCCGGGCCTGGAATGGACAGATTTTTGAAGCTGGGGGT
CAGTTCCGGCCCGAGGTGTCCATGGATGGCTGCAAGGTCCAAGATGGCCGCTGGCATCAG
ACACTCTTCACCTTCCGGACCCAAGACCCCCAACAGCTGCCCATCATCAGTGTGGACAAC
CTCCCTCCTGCCTCATCAGGGAAGCAGTACCGCCTGGAAGTTGGACCTGCGTGCTTCCTC
TTG
Protein sequence predicted based on nucleotide sequence
MGAGSARGARGTAAAAAARGGGFLFSWILVSFACHLASTQGAPEDVDILQRLGLSWTKAG SPAPPGVIPFQSGFIFTQRARLQAPTGTVIPAALGTELALVLSLCSHRVNHAFLFAVRSQ KRKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVP VLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQAD TYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPALGSLPAGRGPRGTVAPATPTKP QRTSPTNPHQHMAVGGPAQTPLLPAKLSASNALDPMLPASVGGSTRTPRPAAAQPSQKIT ATKIPKSLPTKPSAPSTSIVPIKSPHPTQKTAPSSFTKSALPTQKQVPPTSRPVPARVSR PAEKPIQRNPGMPRPPPPSTRPLPPTTSSSKKPIPTLARTEAKITSHASKPASARTSTHK PPPFTALSSSPAPTPGSTRSTRPPATMVPPTSGTSTPRTAPAVPTPGSAPTGSKKPIGSE ASKKAGPKSSPRKPVPLRPGKAARDVPLSDLTTRPSPRQPQPSQQTTPALVLAPAQFLSS SPRPTSSGYSIFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPY GNPGLPGPPGAKGQKGDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHP GEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVP GKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMK GLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSI GFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLE GQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPP GVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSR GLKGPPGPQGRPGRPGQQGVAGERGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPI GPPGEMGPKGPPGAVGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPD GLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAK GYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPL GPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQ QGQPGHPGPRGWPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGI AGPDGLPGRDGQAGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQ LGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGP RGPPGLMGKEGIVGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGG PIQLQQDDLGAAFQTWMDTSGALRPESYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPL GTKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKV EFAISRVQMNFLHLLSSEVTQHITIHCLNMTVWQEGTGQTPAKQAVRFRAWNGQIFEAGG QFRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIISVDNLPPASSGKQYRLEVGPACFL L
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
