Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00865406
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5190nts
Open reading frame: 1 to 5190
ATGAAAGTGTCTACTCTCAGGGAAAGCTCAGCCATGGCTTCCCCACTGCCCCGGGAGATG
GAGGAGGAGCTGGTGCCTACTGGCTCTGAGCCAGGTGACACTCGGGCCAAACCCCCTGTC
AAGCCCAAACCCCGGGCCCTGCCTGCCAAGCCAGCCCTGCCTGCCAAACCCAGCCTGCTG
GTGCCTGTTGGGCCTCGGCCTCCCCGGGGTCCCCTGGCTGAGTTGCCTTCTGCCAGGAAG
ATGAACATGCTGGCAGGACCCCAGCCCTATGGTGGCAGCAAGCGCCCCCTTCCCTTTGCA
CCAAGGCCTGCGGTTGAGGCCTCCACTGGAGGAGAAGCCACCCAAGAGACTGGGAAAGAG
GAGGCTGGGAAAGAGGAGCCACCCCCTTTGACACCCCCAGCTCGATGTGCAGCCCCAGGG
GGTGTACGGAAGGCCCCTGCCCCTTTCCGCCCAGCCTCAGAGCGCTTCGCGGCCACCACG
GTGGAAGAGATCCTGGCCAAGATGGAGCAGCCTCGGAAGGAGGTCCTTGCCAGCCCCGAC
CGCCTGTGGGGTTCCCGCCTCACCTTTAACCACGATGGCAGCTCGCGATATGGCCCCAGG
ACCTATGGCACGACCACTGCTCCCAGGGATGAGGATGGCAGCACCCTCTTCAGGGGATGG
TCCCAGGAGGGGCCAGTAAAGTCTCCAGCAGAGTGCCGGGAAGAGCACAGCAAGACCCCT
GAGGAGAGGAGCCTTCCTTCCGACCTGGCCTTCAACGGGGACCTGGCTAAGGCAGCCAGC
TCGGAGCTACCTGCTGATATTTCCAAGCCCTGGATTCCCTCAAGTCCAGCCCCCTCCTCA
GAGAATGGAGGCCCTGCCAGCCCAGGCCTCCCCGCAGAAGCCTCAGGCTCAGGCCCTGGC
TCTCCCCATCTTCACCCGCCTGATAAGAGTTCTCCCTGCCACTCACAGCTTCTGGAAGCC
CAGACTCCTGAAGCTTCCCAGGCTTCTCCCTGCCCCGCTGTGACTCCATCAGCTCCAAGT
GCAGCCCTGCCTGACGAGGGCTCCCGCCACACCCCCAGCCCGGGGCTCCCTGCCGAGGGG
GCTCCAGAGGCCCCCAGACCCAGCAGCCCACCCCCTGAGGTCTTGGAGCCCCATAGCCTG
GATCAGCCCCCTGCCACCTCACCCCGGCCCCTGATCGAGGTGGGTGAGTTGCTGGATCTC
ACTCGGACGTTTCCATCTGGCGGGGAGGAGGAGGCCAAGGGTGACGCACACCTCCGCCCC
ACCAGCCTGGTTCAGCGCCGATTCTCTGAAGGTGTGCTCCAGTCACCCAGTCAGGACCAG
GAGAAGCTGGGGGGCTCGCTGGCTGCCCTGCCCCAAGGCCAGGGGAGCCAGTTGGCCCTG
GATCGTCCCTTTGGGGCAGAGTCCAACTGGAGCTTATCACAGTCCTTCGAATGGACCTTC
CCCACGAGGCCCTCGGGTCTGGGCGTGTGGCGGCTGGACTCCCCGCCTCCCTCCCCCATC
ACTGAAGCCAGTGAGGCCGCCGAGGCTGCTGAGGCTGGCAACTTGGCCGTTTCCAGCAGG
GAAGAAGGAGTGTCTCAGCAGGGGCAAGGGGCTGGGTCAGCTCCAAGTGGGTCAGGAAGT
TCCTGGGTGCAGGGGGATGATCCAAGCATGTCCCTCACCCAGAAGGGCGATGGGGAGAGT
CAACCTCAATTCCCAGCTGTTCCCCTTGAGCCCCTGCCTACAACTGAGGGCACACCTGGA
TTACCTTTGCAGCAGGCAGAGGAGAGATACGAGTCGCAGGAGCCCTTGGCTGGACAGGAG
TCCCCTCTCCCCCTGGCTACCAGGGAGGCAGCCTTGCCCATCCTGGAGCCAGTCCTGGGG
CAGGAGCAGCCAGCAGCCCCTGACCAGCCCTGTGTTCTCTTTGCTGATGCCCCTGAGCCT
GGACAGGCACTGCCTGTTGAGGAGGAGGCCGTGACCCTAGCCCGGGCTGAGACCACCCAA
GCCAGGACAGAGGCTCAAGACTTGTGTAGGGCATCCCCCGAGCCTCCAGGCCCTGAAAGC
AGCTCCCGCTGGCTGGACGACCTCCTGGCTTCACCACCACCCAGTGGTGGCGGTGCAAGG
CGGGGAGCTGGAGCTGAGCTGAAGGACACACAGTCCCCAAGTACCTGCTCTGAGGGACTC
CTTGGCTGGTCCCAGAAAGATCTGCAGAGTGAATTTGGGATCACAGGAGACCCACAGCCC
AGCAGTTTCAGTCCTTCCAGCTGGTGTCAAGGTGCTTCTCAGGACTATGGCCTTGGGGGT
GCAAGCCCTAGAGGAGACCCAGGTCTCGGAGAGAGGGACTGGACCAGCAAGTATGGGCAA
GGAGCAGGGGAAGGGAGCACCAGGGAGTGGGCCAGCAGGTGTGGCATCGGCCAGGAGGAG
ATGGAGGCCAGCAGCAGCCAAGACCAGAGTAAAGTGTCTGCCCCAGGGGTGCTCACAGCC
CAGGACCGGGTAGTTGGAAAGCCAGCCCAGCTTGGCACTCAGCGGAGCCAGGAGGCAGAT
GTTCAGGACTGGGAGTTCAGAAAGAGGGATTCCCAGGGCACTTACTCCAGCCGGGATGCA
GAACTCCAGGACCAGGAATTCGGAAAGAGAGATTCACTGGGTACCTACAGTAGTCGAGAT
GTAAGCCTTGGGGACTGGGAATTTGGGAAGAGAGATTCTCTGGGTGCTTATGCCAGCCAA
GATGCCAACGAGCAGGGCCAAGATTTGGGGAAGAGGGACCACCATGGTAGGTACAGCAGC
CAGGATGCCGATGAGCAGGACTGGGAGTTTCAGAAGAGAGATGTGTCACTCGGCACCTAT
GGCAGCCGGGCTGCGGAGCCACAGGAACAGGAGTTTGGGAAGAGCGCTTGGATAAGGGAC
TACAGCAGTGGTGGCAGCTCCAGGACCCTTGACGCCCAGGACAGAAGCTTTGGAACGAGA
CCCCTGAGCTCTGGGTTCAGCCCCGAGGAAGCCCAGCAACAGGATGAGGAATTTGAGAAG
AAGATTCCAAGTGTGGAAGACAGCCTTGGAGAGGGCAGCAGGGATGCTGGCCGGCCAGGA
GAGAGAGGATCCGGGGGCTTGTTCAGTCCTAGCACTGCCCACGTGCCGGATGGGGCACTC
GGGCAGAGAGACCAGAGCAGCTGGCAAAACAGTGATGCTAGCCAGGAGGTGGGAGGGCAT
CAGGAGAGACAGCAGGCAGGGGCTCAGGGCCCTGGCAGTGCTGACCTGGAAGATGGGGAG
ATGGGAAAGCGAGGCTGGGTCGGTGAGTTTAGCCTCAGTGTTGGCCCCCAGCGAGAGGCA
GCATTTAGCCCAGGGCAGCAGGACTGGAGCCGGGACTTCTGCATCGAGGCCAGTGAGAGG
AGCTATCAGTTTGGCATCATTGGCAACGACAGAGTGAGTGGTGCTGGCTTTAGCCCTTCT
AGCAAGATGGAAGGTGGTCACTTTGTGCCTCCTGGGAAGACCACAGCTGGCTCGGTGGAC
TGGACTGACCAGCTGGGTCTCAGGAACTTGGAAGTGTCCAGCTGTGTGGGTTCTGGGGGC
TCGAGCGAGGCCAGGGAGAGTGCCGTGGGACAGATGGGCTGGTCAGGTGGCCTGAGCTTG
AGAGACATGAACCTGACCGGCTGTTTGGAAAGTGGAGGGTCTGAAGAGCCGGGGGGAATC
GGAGTTGGGGAGAAGGACTGGACTTCTGATGTTAATGTGAAGAGCAAAGATTTGGCTGAG
GTCGGGGAGGGAGGAGGCCACAGCCAGGCCAGAGAGAGTGGCGTGGGGCAGACTGACTGG
TCAGGTGTGGAGGCCGGAGAGTTCCTTAAATCAAGGGAGCGTGGAGTTGGACAGGCAGAC
TGGACACCTGACCTTGGGCTGAGAAACATGGCCCCAGGGGCAGTCTGCAGTCCTGGAGAG
TCCAAAGAGCTTGGGGTGGGCCAGATGGACTGGGGTAACAATCTGGGCCTGAGGGATTTG
GAGGTGACCTGTGACCCAGACTCTGGAGGTTCTCAGGGGCTACGGGGATGTGGAGTGGGG
CAGATGGACTGGACCCAGGACTTGGCGCCCCAGAATGTGGAGCTCTTTGGGGCTCCAAGT
GAAGCCAGGGAGCATGGGGTGGGCGGGGTGAGCCAGTGCCCAGAGCCCGGCCTGAGGCAC
AATGGCAGCTTGTCTCCTGGCCTGGAGGCCAGAGACCCCTTGGAGGCCAGGGAGCTGGGG
GTTGGTGAGACAAGTGGGCCAGAGACCCAGGGTGAAGATTACTCCTCGTCTTCCTTGGAG
CCACACCCTGCAGACCCTGGAATGGAGACAGGAGAAGCCCTCAGCTTCGGAGCAAGCCCT
GGCAGGTGCCCGGCCCGCCCCCCACCCTCCGGCTCCCAGGGCCTGCTGGAGGAGATGCTG
GCAGCCAGCAGCTCCAAGGCGGTGGCTCGGAGGGAGTCAGCGGCCTCGGGCCTTGGGGGC
CTGTTGGAGGAGGAAGGAGCCGGGGCAGGTGCTGCCCAAGAGGAGGTGCTGGAGCCTGGC
AGGGACTCTCCACCCTCCTGGAGGCCGCAGCCTGATGGTGAGGCCAGCCAGACAGAAGAC
GTGGATGGCACCTGGGGCTCTTCAGCAGCCAGGTGGAGCGATCAGGGGCCAGCACAGACT
TCTCGGCGACCCTCCCAAGGCCCTCCTGCCAGATCCCCCAGTCAGGACTTCTCCTTCATT
GAGGACACCGAGATCCTCGACAGTGCCATGTATCGGAGCCGTGCCAACTTGGGGCGCAAG
CGTGGGCACCGGGCCCCGGTCATTCGGCCTGGGGGTACCTTGGGCCTGTCGGAGGCAGCA
GACTCGGATGCACACCTGTTCCAGGACTCTACAGAGCCACGGGCATCTCGGGTGCCATCT
TCAGATGAAGAGGTAGTGGAGGAACCTCAGAGCCGCCGGACACGGATGTCGTTGGGCACC
AAGGGGCTGAAAGTCAACCTCTTTCCTGGCCTGAGCCCCTCAGCCCTGAAGGCCAAGCTG
CGCCCCCGGAATCGCTCAGCTGAGGAGGGAGAGCTGGCTGAGAGCAAGTCGAGCCAGAAG
GAGTCCGCGGTCCAGCGTTCGAAATCCTGCAAGGTCCCAGGACTGGGAAAGCCCCTCACG
TTACCTCCCAAGCCAGAGAAATCCTCAGGGTCAGAAGGATCGTCGCCCAACTGGCTTCAA
GCCCTGAAACTGAAGAAGAAGAAGGTCTTG
Protein sequence predicted based on nucleotide sequence
MKVSTLRESSAMASPLPREMEEELVPTGSEPGDTRAKPPVKPKPRALPAKPALPAKPSLL VPVGPRPPRGPLAELPSARKMNMLAGPQPYGGSKRPLPFAPRPAVEASTGGEATQETGKE EAGKEEPPPLTPPARCAAPGGVRKAPAPFRPASERFAATTVEEILAKMEQPRKEVLASPD RLWGSRLTFNHDGSSRYGPRTYGTTTAPRDEDGSTLFRGWSQEGPVKSPAECREEHSKTP EERSLPSDLAFNGDLAKAASSELPADISKPWIPSSPAPSSENGGPASPGLPAEASGSGPG SPHLHPPDKSSPCHSQLLEAQTPEASQASPCPAVTPSAPSAALPDEGSRHTPSPGLPAEG APEAPRPSSPPPEVLEPHSLDQPPATSPRPLIEVGELLDLTRTFPSGGEEEAKGDAHLRP TSLVQRRFSEGVLQSPSQDQEKLGGSLAALPQGQGSQLALDRPFGAESNWSLSQSFEWTF PTRPSGLGVWRLDSPPPSPITEASEAAEAAEAGNLAVSSREEGVSQQGQGAGSAPSGSGS SWVQGDDPSMSLTQKGDGESQPQFPAVPLEPLPTTEGTPGLPLQQAEERYESQEPLAGQE SPLPLATREAALPILEPVLGQEQPAAPDQPCVLFADAPEPGQALPVEEEAVTLARAETTQ ARTEAQDLCRASPEPPGPESSSRWLDDLLASPPPSGGGARRGAGAELKDTQSPSTCSEGL LGWSQKDLQSEFGITGDPQPSSFSPSSWCQGASQDYGLGGASPRGDPGLGERDWTSKYGQ GAGEGSTREWASRCGIGQEEMEASSSQDQSKVSAPGVLTAQDRVVGKPAQLGTQRSQEAD VQDWEFRKRDSQGTYSSRDAELQDQEFGKRDSLGTYSSRDVSLGDWEFGKRDSLGAYASQ DANEQGQDLGKRDHHGRYSSQDADEQDWEFQKRDVSLGTYGSRAAEPQEQEFGKSAWIRD YSSGGSSRTLDAQDRSFGTRPLSSGFSPEEAQQQDEEFEKKIPSVEDSLGEGSRDAGRPG ERGSGGLFSPSTAHVPDGALGQRDQSSWQNSDASQEVGGHQERQQAGAQGPGSADLEDGE MGKRGWVGEFSLSVGPQREAAFSPGQQDWSRDFCIEASERSYQFGIIGNDRVSGAGFSPS SKMEGGHFVPPGKTTAGSVDWTDQLGLRNLEVSSCVGSGGSSEARESAVGQMGWSGGLSL RDMNLTGCLESGGSEEPGGIGVGEKDWTSDVNVKSKDLAEVGEGGGHSQARESGVGQTDW SGVEAGEFLKSRERGVGQADWTPDLGLRNMAPGAVCSPGESKELGVGQMDWGNNLGLRDL EVTCDPDSGGSQGLRGCGVGQMDWTQDLAPQNVELFGAPSEAREHGVGGVSQCPEPGLRH NGSLSPGLEARDPLEARELGVGETSGPETQGEDYSSSSLEPHPADPGMETGEALSFGASP GRCPARPPPSGSQGLLEEMLAASSSKAVARRESAASGLGGLLEEEGAGAGAAQEEVLEPG RDSPPSWRPQPDGEASQTEDVDGTWGSSAARWSDQGPAQTSRRPSQGPPARSPSQDFSFI EDTEILDSAMYRSRANLGRKRGHRAPVIRPGGTLGLSEAADSDAHLFQDSTEPRASRVPS SDEEVVEEPQSRRTRMSLGTKGLKVNLFPGLSPSALKAKLRPRNRSAEEGELAESKSSQK ESAVQRSKSCKVPGLGKPLTLPPKPEKSSGSEGSSPNWLQALKLKKKKVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
