Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00865418
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 6486nts
Open reading frame: 1 to 6486
ATGACCCACAGCCCCGCGACAAGCGAGGACGAGGAACGCCACAGTGCCAGCGAGTGTCCC
GAGGGGGGCTCAGAGTCCGACAGCTCCCCAGACGGGCCAGGTCGAGGCCCCCGGGGGACC
CGGGGCCAGGGCAGTGGGGCACCTGGTAGCCTGGCCTCTGTTAGAGGCCTCCAGGGCCGC
TCAATGTCCGTCCCAGACGACGCCCACTTCAGCATGATGGTCTTCAGGATTGGCATCCCG
GACCTGCACCAGACAAAATGCCTTCGCTTCAACCCCGATGCCACCATCTGGACGGCCAAG
CAGCAGGTGCTCTGTGCCCTGAGCGAGAGCCTGCAGGATGTGCTCAACTATGGCCTGTTC
CAACCGGCCACCTCCGGCCGCGATGCCAACTTCCTGGAGGAGGAGAGGCTGCTGCGGGAG
TACCCCCAGTCCTTTGAGAAGGGGGTCCCCTACCTGGAGTTCCGATACAAGACCCGAGTT
TACAAACAGACCAACCTGGATGAGAAGCAGCTGGCCAAGTTGCACACGAAGACGGGGTTG
AAGAAGTTCCTGGAGTATGTGCAGCTCGGGACATCTGACAAGGTGGCGCGGCTGCTGGAC
AAGGGGCTGGACCCCAATTACCATGACTCGGATTCGGGAGAGACCCCCTTGACACTGGCG
GCCCAGACCGAAGGCTCTGTAGAGGTGATTCGAACCCTGTGCCTGGGCGGGGCCCACATT
GACTTCCGGGCCCGGGATGGCATGACCGCACTGCATAAGGCCGCATGCGCCCGACACTGC
CTGGCACTCACGGCGCTCCTGGACCTTGGGGGTTCCCCCAACTACAAGGACCGTCGGGGG
CTGACCCCTCTGTTCCACACGGCCATGGTGGGTGGTGACCCCCGATGCTGCGAGCTGCTC
CTGTTCAACAGGGCCCAGCTGGGCATAGCTGATGAGAACGGCTGGCAGGAAATCCACCAG
GCCTGCCAGCGGGGTCACTCTCAGCACCTGGAGCATCTGCTTTTCTACGGGGCTGAGCCT
GGAGCCCAGAACGCCTCGGGGAACACGGCTCTGCACATCTGCGCCCTCTACAACAAGGAG
ACCTGTGCCAGGATCCTCCTGTATCGAGGTGCCGACAAGGATGTGAAGAACAACAACGGA
CAGACCCCCTTCCAGGTGGCAGTGATTGCTGGGAATTTTGAGCTGGGGGAGCTGATCCGA
AACCACCGAGAACAGGATGTGGTGCCCTTCCAGGAGTCCCCCAAGTACGCGGCCCGGCGA
CGGGGGCCCCCAGGCACAGGGCTGACGGTGCCCCCGGCGCTGCTGCGGGCCAACAGTGAC
ACCAGCATGGCGCTGCCCGACTGGATGGTGTTCTCCGCCCCGGGGGCCGCGTCCTCTGGG
GCCCCTGGCCCTACCTCAGGGTCCCAGGGCCAGTCGCAGCCCTCGGCCCCCACCACCAAG
CTCAGCAGCGGGACCCTCCGAAGTGCCAGCAGCCCCCGGGGTGCCAGGGCCCGCTCTCCA
TCCCGAGGGAGGCACCCTGAGGACGCCAAGAGGCAGCCCCGAGGCCGGCCCAGCTCCAGC
GGGACACCCCGGGAAGGGCCAGCCGGGGGCACGGGGGGCTCAGGGGGCCCCGGGGGCTCC
CTGGGCAGCCGCGGGAGGCGGAGGAAGCTCTACTCAGCGGTACCCGGACGCTCCTTCATG
GCTGTGAAGTCCTACCAGGCCCAAGCCGAGGGGGAGATCTCCCTGAGCAAGGGCGAGAAG
ATCAAAGTACTTAGCATCGGGGAAGGAGGCTTCTGGGAAGGCCAGGTCAAAGGTCGTGTT
GGCTGGTTCCCCTCTGACTGCCTGGAAGAAGTGGCGAATCGCTCTCAGGAGAGCAAGCAA
GAAAGCCGCAGTGACAAGGCAAAGAGACTCTTCCGGCATTATACCGTGGGCTCCTACGAC
AGCTTTGATGCCCCAAGCTTAATGGATGGGATTGGCCCAGGGAGCGATTACATCATTAAG
GAGAAGACAGTCTTGCTGCAGAAGAAGGACAGTGAGGGGTTTGGGTTCGTGCTCCGGGGG
GCCAAGGCGCAGACCCCCATCGAGGAGTTCACCCCCACCCCGGCCTTCCCGGCGCTGCAG
TACCTGGAGTCGGTGGACGAGGGTGGCGTGGCATGGCGAGCTGGACTGCGAATGGGAGAC
TTCCTCATCGAGGTGAACGGGCAGAATGTGGTGAAGGTCGGCCACCGACAGGTGGTGAAC
ATGATCCGCCAAGGGGGCAACACGCTGATGGTGAAGGTGGTGATGGTCACCAGGCACCCG
GACATGGATGAGGCAGTGCACAAGAAAGCACCCCAGCAGGCCAAGCGGCTGCCGCCCCCA
ACCATCTCCCTGCGTTCCAAATCTATGACCTCAGAGCTGGAGGAGATGGAGTACGAGCAG
CAGCCGGCGCCGGTGCCCAGCATGGAGAAAAAGCGGACCGTGTATCAGATGGCTCTCAAC
AAACTGGACGAAATCCTGGCCGCAGCTCAACAGACCATCAGTGCAAGCGAAAGCCCTGGT
CCCGGTGGCCTCGCGTCCCTGGGCAAACACCGACCCAAAGGTTTCTTTGCCACTGAGTCG
AGCTTCGATCCCCACCACCGTGCCCAGCCAAGTTACGAGCGTCCTTCTTTCCTGCCTCCA
GGACCTGGGTTGATGCTCCGGCAAAAATCTATCGGTGCGGCAGAAGATGACAGACCTTAC
CTAGCACCCCCAGCCATGAAATTCAGCCGCAGCCTGTCTGTGCCTGGTTCGGAGGACATT
CCCCCGCCACCCACCACGTCCCCACCGGAGCCTCCCTACAGCACACCTCCAGTCCCCTCC
TCCTCAGGGCGCCTCACCCCCTCCCCTCGGGGAGGGCCCTTCAACCCTGGCTCTGGTGGC
CCCCTCCCCGCCTCCTCCCCTGCATCCTTTGACGGGCCCTCCCCTCCCGACACTCGCGTG
GGGAGCCGCGAGAAGAGCCTGTACCACAGTGGGCCCCTGCCCCCGGCCCACCACCACCCG
CCCCACCACCACCACCACCACGCCCCGCCCCCTCAGCCCCACCACCACCACGCCCACCCC
CCTCATCCTCCCGAGATGGAGACAGGCGGCTCTCCCGACGACCCTCCACCCCGCCTGGCT
CTGGGGCCCCAGCCCAGCCTGCGAGGCTGGAGGGGCGGCGGGCCCAGCCCGACCCCGGGG
GCCCCGTCCCCATCGCACCACGGCAGCGCGGGCGGGGGCGGCGGCTCCTCCCAGGGCCCG
GCTCTACGCTATTTCCAGCTGCCCCCGCGGGCGGCCAGCGCAGCCATGTACGTGCCCGCC
CGCTCGGGCCGCGGCCGCAAGGGCCCGCTGGTCAAGCAGACCAAGGTGGAAGGCGAGCCC
CAGAAGGGCGGCGGCCTCCCGCCCGCGCCGTCGCCCACGTCCCCGGCCTCCCCGCAGCCG
CCGCCCGCCGTGGCCGCGCCCTCGGAGAAGAACTCCATCCCCATCCCCACCATCATCATC
AAGGCCCCGTCCACCAGTAGCAGCGGCCGCAGCAGCCAGGGCAGCAGCACCGAGGCGGAG
CCCCCCACCCAGCCGGAGCCCACGGGAGGCGGCGGCGGCGGCGGCTCCTCGCCCAGCCCC
GCCCCGGCCATGTCACCCGTGCCCCCGTCCCCCTCGCCCGTGCCCACCCCCGCCTCGCCC
AGCGGCCCGGCCACGCTGGACTTCACGAGCCAGTTCGGGGCCGCCCTGGTGGGGGCGGCC
CGGAGGGAGGGGGGCTGGCAGAATGAGGCGCGCCGGCGCTCCACGCTGTTCCTGTCCACC
GACGCGGGGGACGAGGACGGCGGGGACGGCGGGCTGGGCACAGGGGCGGCCCCGGGCCCG
CGGCTGCGCCACTCCAAATCCATCGACGAGGGCATGTTCTCCGCCGAGCCCTACCTCCGA
CTGGAGTCTGCGGGCAGCGGCGCGGGCTACGGCGGCTACGGGGCCGGTAGCCGAGCCTAC
GGGGGTGGCGGGGGCAGCAGCGCCTTCACCAGCTTCCTGCCCCCGCGACCCCTGGTGCAC
CCGCTGACCGGCAAGGCCCTGGATCCCGCCTCCCCGCTGGGGCTGGCCCTGGCCGCCCGC
GAGCGAGCGCTGAAGGAGTCCTCGGAGGGCGGCGGGGCCCCCCAGCCGCCTCCCAGGCCC
CCATCGCCCCGCTACGAGGCCCCGCCGCCCACCCCGCACCACCACTCGCCCCACGCCCAC
CACGAGCCAGTGCTGCGTCTCTGGGGGGCCTCCCCGCCGGACCCTGCGCGCCGGGAGCTG
GGGTACAGGGCCGGGCTGGGCAGCCAGGAGAAGTCCCTTCCCGCCAGCCCGCCCGCCGCC
CGGCGTTCCCTGCTACACCGCCTGCCGCCCACCGCTCCCGGGGTGGGGCCCCTCCTGCTG
CAGCTGGGGACGGAGCCCCCGGCCCCGCACCCCGGAGTAAGCAAGCCCTGGAGGTCCGCA
GCCCCCGAAGAACCCGAGCGGCTGCCGCTGCACGTGCGGTTCCTTGAAAACTGCCAGCCC
CGGGCCCCTGTGACGAGCGGAAGGGGTCCCCCCTCGGAGGACGGGCCGGGGGTCCCGCCG
CCCAGCCCACGCCGGTCCGTGCCCCCCTCCCCGACCTCCCCGAGGGCCAGCGAAGAGAAC
GGGCTGCCCCTGCTGGTCCTGCCGCCTCCCGCCCCCTCGGTGGATGTGGAAGATGGCGAA
TTCCTTTTCGTGGAACCGCTGCCTCCGCCTCTGGAATTCTCCAACAGCTTCGAAAAGCCA
GAGTCGCCCCTCACGCCTGGGCCTCCCCACCCGCTGCCCGACACACCTGCCCCTGCCACC
CCGTTACCCCCTGTGCCACCCCCGGCTGTGGCCGCAGCCCCTCCCACCCTGGACTCCACC
GCATCCAGCCTGACATCCTATGACAGCGAGGTGGCCACCCTGACCCAGGGGGCCTCCGCC
GCTCCTGGGGACCCCCATCCACCAGGCCCGCCTGCCCCAGCAGCACCGGCTCCCGCTGCC
CCACAGCCTGGCCCGGACCCTCCGCCTGGCACGGATTCTGGCATCGAGGAGGTGGACAGT
CGGAGCAGCAGTGACCACCCACTGGAGACCATCAGCAGCGCCTCCACGCTGAGCAGCCTA
TCTGCCGAAGGTGGTGGCAGCGCAGGGGGTGGGGGCGGGGCTGGGGCCGGTGTGGCCAGT
GGGCCGGAGCTTCTGGACACCTATGTGGCCTACCTGGACGGCCAGGCCTTTGGGGGCAGC
AGTACTCCCGGCCCGCCATACCCTCCTCAGCTCATGACTCCCTCTAAGCTCCGGGGCCGG
GCGCTAGGAGCCAGCGGAGGCCTGCGGCCTGGCCCCAGCGGGGGACTCCGAGACCCTGTT
ACCCCCACCAGCCCCACCGTCTCGGTGACAGGGGCTGGAACCGATGGGCTGCTGGCCCTG
CGTGCTTGTTCAGGACCCCCCACGGCAGGCGTGGCGGGGGGTCCGGTGGCTGTAGAGCCA
GAAGTCCCACCGGTGCCCTTGCCGACGGCCTCCTCTCTGCCCCGGAAGCTGCTGCCCTGG
GAGGAGGGCCCGGGCCCACCGCCACCACCTCTGCCCGGGCCCTTGGCCCAGCCTCAGGCC
TCAGCCTTGGCCACAGTAAAAGCCAGCATCATCAGTGAACTCAGCTCCAAGCTTCAGCAG
TTTGGGGGCTCCTCGGCAGCTGGCGGCGCTCTGCCCTGGGCCCGAGGAGGCAGTGGGGGA
GGCGGAGACAGCCACCACGGGGGAGCCAGCTATGTCCCCGAGAGGACCTCCTCCCTGCAG
CGGCAGAGACTCTCCGACGACTCCCAGTCCTCACTCCTCTCCAAGCCTGTCAGCAGCCTG
TTTCAGAACTGGCCCAAACCACCTCTGCCGCCACTCCCCACCGGAACAGGGGTCTCCCCT
ACAGCCGCTGCGGCCCCAGGGGCCACCTCACCCTCAGCCTCCTCCTCCTCCACGTCCACC
CGCCACCTCCAGGGCGTGGAGTTCGAGATGCGGCCCCCTCTGCTCCGCCGGGCCCCCAGC
CCCTCGCTGCTGCCCGCCTCGGAGCACAAGGTCAGCCCTGCGCCCAGGCCCTCGTCCCTG
CCCATCCTGCCTTCCGGACCCCTCTACCCAGGCCTCTTTGACATCCGTGGCTCCCCAACT
GGAGGGGCAGGAGGCTCGGCTGACCCCTTCGCCCCAGTCTTTGTGCCGCCACACCCGGGG
ATATCCGGGGGGCTCGGGGGAGCCTTGTCAGGGGCCTCGCGCTCCCTCTCACCGACCCGC
CTGCTCTCGCTGCCCCCGGACAAGCCGTTTGGCGCTAAACCTCTGGGGTTCTGGACCAAG
TTCGACGTGGCTGATTGGCTGGAGTGGCTGGGTTTGGCGGAGCACCGAGCCCAGTTCCTG
GACCACGAGATCGATGGCTCCCACCTGCCCGCCTTGACCAAGGAGGACTACGTCGATCTA
GGTGTGACCAGGGTGGGCCACCGCATGAACATCGACCGGGCTCTCAAATTCTTCCTGGAG
AGGTTG
Protein sequence predicted based on nucleotide sequence
MTHSPATSEDEERHSASECPEGGSESDSSPDGPGRGPRGTRGQGSGAPGSLASVRGLQGR SMSVPDDAHFSMMVFRIGIPDLHQTKCLRFNPDATIWTAKQQVLCALSESLQDVLNYGLF QPATSGRDANFLEEERLLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKTGL KKFLEYVQLGTSDKVARLLDKGLDPNYHDSDSGETPLTLAAQTEGSVEVIRTLCLGGAHI DFRARDGMTALHKAACARHCLALTALLDLGGSPNYKDRRGLTPLFHTAMVGGDPRCCELL LFNRAQLGIADENGWQEIHQACQRGHSQHLEHLLFYGAEPGAQNASGNTALHICALYNKE TCARILLYRGADKDVKNNNGQTPFQVAVIAGNFELGELIRNHREQDVVPFQESPKYAARR RGPPGTGLTVPPALLRANSDTSMALPDWMVFSAPGAASSGAPGPTSGSQGQSQPSAPTTK LSSGTLRSASSPRGARARSPSRGRHPEDAKRQPRGRPSSSGTPREGPAGGTGGSGGPGGS LGSRGRRRKLYSAVPGRSFMAVKSYQAQAEGEISLSKGEKIKVLSIGEGGFWEGQVKGRV GWFPSDCLEEVANRSQESKQESRSDKAKRLFRHYTVGSYDSFDAPSLMDGIGPGSDYIIK EKTVLLQKKDSEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGD FLIEVNGQNVVKVGHRQVVNMIRQGGNTLMVKVVMVTRHPDMDEAVHKKAPQQAKRLPPP TISLRSKSMTSELEEMEYEQQPAPVPSMEKKRTVYQMALNKLDEILAAAQQTISASESPG PGGLASLGKHRPKGFFATESSFDPHHRAQPSYERPSFLPPGPGLMLRQKSIGAAEDDRPY LAPPAMKFSRSLSVPGSEDIPPPPTTSPPEPPYSTPPVPSSSGRLTPSPRGGPFNPGSGG PLPASSPASFDGPSPPDTRVGSREKSLYHSGPLPPAHHHPPHHHHHHAPPPQPHHHHAHP PHPPEMETGGSPDDPPPRLALGPQPSLRGWRGGGPSPTPGAPSPSHHGSAGGGGGSSQGP ALRYFQLPPRAASAAMYVPARSGRGRKGPLVKQTKVEGEPQKGGGLPPAPSPTSPASPQP PPAVAAPSEKNSIPIPTIIIKAPSTSSSGRSSQGSSTEAEPPTQPEPTGGGGGGGSSPSP APAMSPVPPSPSPVPTPASPSGPATLDFTSQFGAALVGAARREGGWQNEARRRSTLFLST DAGDEDGGDGGLGTGAAPGPRLRHSKSIDEGMFSAEPYLRLESAGSGAGYGGYGAGSRAY GGGGGSSAFTSFLPPRPLVHPLTGKALDPASPLGLALAARERALKESSEGGGAPQPPPRP PSPRYEAPPPTPHHHSPHAHHEPVLRLWGASPPDPARRELGYRAGLGSQEKSLPASPPAA RRSLLHRLPPTAPGVGPLLLQLGTEPPAPHPGVSKPWRSAAPEEPERLPLHVRFLENCQP RAPVTSGRGPPSEDGPGVPPPSPRRSVPPSPTSPRASEENGLPLLVLPPPAPSVDVEDGE FLFVEPLPPPLEFSNSFEKPESPLTPGPPHPLPDTPAPATPLPPVPPPAVAAAPPTLDST ASSLTSYDSEVATLTQGASAAPGDPHPPGPPAPAAPAPAAPQPGPDPPPGTDSGIEEVDS RSSSDHPLETISSASTLSSLSAEGGGSAGGGGGAGAGVASGPELLDTYVAYLDGQAFGGS STPGPPYPPQLMTPSKLRGRALGASGGLRPGPSGGLRDPVTPTSPTVSVTGAGTDGLLAL RACSGPPTAGVAGGPVAVEPEVPPVPLPTASSLPRKLLPWEEGPGPPPPPLPGPLAQPQA SALATVKASIISELSSKLQQFGGSSAAGGALPWARGGSGGGGDSHHGGASYVPERTSSLQ RQRLSDDSQSSLLSKPVSSLFQNWPKPPLPPLPTGTGVSPTAAAAPGATSPSASSSSTST RHLQGVEFEMRPPLLRRAPSPSLLPASEHKVSPAPRPSSLPILPSGPLYPGLFDIRGSPT GGAGGSADPFAPVFVPPHPGISGGLGGALSGASRSLSPTRLLSLPPDKPFGAKPLGFWTK FDVADWLEWLGLAEHRAQFLDHEIDGSHLPALTKEDYVDLGVTRVGHRMNIDRALKFFLE RL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
